研究支援推進本部

尾之内 高慶

オノウチ タカノリ  (onouchi takanori)

基本情報

所属
藤田医科大学 研究推進本部 総合医科学研究部門 オープンファシリティセンター 生体画像解析室 講師
学位
博士(医学)(藤田保健衛生大学)

研究者番号
20632954
J-GLOBAL ID
201501018919214255
researchmap会員ID
7000012692

委員歴

 2

論文

 26
  • Kana Aihara, Yosuke Nakazawa, Shun Takeda, Natsuko Hatsusaka, Takanori Onouchi, Noriko Hiramatsu, Mayumi Nagata, Noriaki Nagai, Megumi Funakoshi-Tago, Naoki Yamamoto, Hiroshi Sasaki
    Medical molecular morphology 2023年7月26日  査読有り
    Regulation of ion and water microcirculation within the lens is tightly controlled through aquaporin channels and connexin junctions. However, cataracts can occur when the lens becomes cloudy. Various factors can induce cataracts, including diabetes which is a well-known cause. The most common phenotype of diabetic cataracts is a cortical and/or posterior subcapsular opacity. In addition to the three main types and two subtypes of cataracts, a vacuole formation is frequently observed; however, their origin remains unclear. In this study, we focused on the aquaporins and connexins involved in diabetes-induced cataracts and vacuoles in Nile grass type II diabetes. The results showed that the expression of aquaporin 0 and aquaporin 5 increased, and that of connexin 43 decreased in diabetic rat lenses. Additionally, aquaporin 0 and 5 were strongly localized in peripheral of vacuoles, suggesting that aquaporins are involved in vacuoles formation. Transillumination photography revealed large vacuoles at the tip of the Y-suture in the anterior capsule of the diabetic lens, and several small vacuoles were observed in the posterior capsule. Within the vacuoles, cytoplasmic degradation and aggregation of fibrous material were observed. Our findings suggest that aquaporins are potential candidate proteins for preventing vacuole formation.
  • Atsuko Sugimoto, Takahiro Watanabe, Kazuhiro Matsuoka, Yusuke Okuno, Yusuke Yanagi, Yohei Narita, Seiyo Mabuchi, Hiroyuki Nobusue, Eiji Sugihara, Masaya Hirayama, Tomihiko Ide, Takanori Onouchi, Yoshitaka Sato, Teru Kanda, Hideyuki Saya, Yasumasa Iwatani, Hiroshi Kimura, Takayuki Murata
    Microbiology Spectrum 2023年7月6日  査読有り
    EBV infections cause nucleolar enlargement via the induction of IMPDH2, which are essential for B cell growth transformation by EBV. Although the significance of IMPDH2 induction and nuclear hypertrophy in the tumorigenesis of glioblastoma has been reported, EBV infection brings about the change quickly by using its transcriptional cofactor, EBNA2, and MYC. Moreover, we present here, for the novel, basic evidence that an IMPDH2 inhibitor, namely, MPA or MMF, can be used for EBV-positive posttransplant lymphoproliferative disorder (PTLD).
  • Masaya Hirayama, Takanori Onouchi, Kazuya Shiogama, Yoshimitsu Katoh, Kazuo Takahashi, Masato Abe
    Microscopy (Oxford, England) 71(2) 87-92 2021年12月1日  査読有り
    Huntingtin-associated protein 1 (HAP1) is abundantly expressed in the neurons of the central nervous system and forms unique intracytoplasmic inclusions of unknown function called "stigmoid bodies" (STBs). Transmission electron microscopy has revealed that the STBs are aggregates of granules containing cavities with a diameter of 0.5-3 µm. Small STBs fuse to form larger STBs, the size of which is said to vary depending on the developmental growth stage and brain region. Light microscopy can only reveal that these STBs have similar circular shapes, due to its limited resolution. Therefore, light microscopy is only fit for the study of the STB distribution and quantitative changes. We, herein, suggest the adoption of correlative light and electron microscopy, that combines confocal laser scanning microscopy and scanning electron microscopy, as the method allowing us to identify the HAP1-positive STBs in formalin-fixed paraffin-embedded (FFPE) sections. This approach allows us to study the three-dimensional morphology of immunolabeled objects in histopathological specimens. The STBs in FFPE sections of murine hypothalami reflected the transmission electron microscopic images of Epon-embedded STBs, although we were not able to observe any organelle covering the STBs of the FFPE sections. Furthermore, we were able to reconstruct the three-dimensional structure of the STB and we identified it to be of spherical form, covered with mitochondria and rough endoplasmic reticulum, and bearing a cluster of cavities in the center. In the future, we might gain new insights by comparing the 3D structure of the STB between different neurons and under a variety of conditions.
  • Yu Kato, Osamu Nishida, Naohide Kuriyama, Tomoyuki Nakamura, Takahiro Kawaji, Takanori Onouchi, Daisuke Hasegawa, Yasuyo Shimomura
    International journal of molecular sciences 22(9) 2021年5月6日  査読有り
    Neutrophil extracellular trap (NET) formation, an innate immune system response, is associated with thrombogenesis and vascular endothelial injury. Circulatory disorders due to microvascular thrombogenesis are one of the principal causes of organ damage. NET formation in organs contributes to the exacerbation of sepsis, which is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection. We have previously reported that recombinant human soluble thrombomodulin (rTM) reduces lipopolysaccharide (LPS)-induced NET formation in vitro. Here, we aimed to show that thrombomodulin (TM)-mediated suppression of NET formation protects against organ damage in sepsis. Mice were injected intraperitoneally (i.p.) with 10 mg/kg LPS. rTM (6 mg/kg/day) or saline was administered i.p. 1 h after LPS injection. In the LPS-induced murine septic shock model, extracellular histones, which are components of NETs, were observed in the liver and lungs. In addition, the serum cytokine (interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemotactic protein-1 (MCP-1), and interleukin-10 (IL-10)) levels were increased. The administration of rTM in this model prevented NET formation in the organs and suppressed the increase in the levels of all cytokines except IL-1β. Furthermore, the survival rate improved. We provide a novel role of TM in treating inflammation and NETs in organs during sepsis.
  • Chenguang Li, Takanori Onouchi, Masaya Hirayama, Kazuyoshi Sakai, Shuji Matsuda, Nami O Yamada, Takao Senda
    Medical molecular morphology 54(1) 31-40 2020年6月22日  査読有り
    In the present study, we examined morphology and function of hippocampus in the APC1638T/1638T mouse. Expression levels of the APC mRNA and protein were both identical in the hippocampus of the APC+/+ and APC1638T/1638T mice. The dentate gyrus of the APC1638T/1638T hippocampus was thicker, and has more densely-populated granule cells in the APC1638T/1638T mouse hippocampus. Immunoelectron microscopy revealed co-localization of APC with alpha-amino-3- hydroxy-5-methyl- isoxazole-4-propionate receptor (AMPA-R) and with PSD-95 at post-synapse in the APC+/+ hippocampus, while APC1638T was co-localized with neither AMPA-R nor PSD-95 in the APC1638T/1638T hippocampus. By immunoprecipitation assay, full-length APC expressed in the APC+/+ mouse was co-immunoprecipitated with AMPA-R and PSD-95. In contrast, APC1638T expressed in the APC1638T/1638T mouse was not co-immunoprecipitated with AMPA-R and PSD-95. In the hippocampal CA1 region of the APC1638T/1638T mouse, c-Fos expression after electric foot shock was decreased compared with the APC+/+ mouse. The present study showed some abnormalities on morphology of the hippocampus caused by a truncated APC (APC1638T). Also, our findings suggest that failure in APC binding to AMPA-R and PSD-95 may bring about less activities of hippocampal neurons in the APC1638T/1638T mouse.

MISC

 113

書籍等出版物

 4

講演・口頭発表等

 81

担当経験のある科目(授業)

 12

共同研究・競争的資金等の研究課題

 6

その他

 2

教育内容・方法の工夫(授業評価等を含む)

 3
  • 件名
    医学部(4年):病態病理実習
    開始年月日
    2012
    終了年月日
    2013
    概要
    医学部M4病態病理実習(16時間)担当
  • 件名
    医学部(2年):病理学実習
    開始年月日
    2012
    終了年月日
    2013
    概要
    医学部M2病理学実習(16時間)担当
  • 件名
    医療科学部臨床検査学科(4年):卒業研究
    開始年月日
    2012
    終了年月日
    2013
    概要
    2012年の研究課題:免疫染色標本の伸展および乾燥条件が染色性に与える影響、2013年の研究課題:好中球細胞外トラップ(Neutrophil Extracellular Traps) -パラフィン切片を用いた組織化学-