研究者業績
基本情報
経歴
1-
2013年 - 現在
論文
85-
日本小児外科学会雑誌 54(7) 1351-1356 2018年12月 査読有り小児期の十二指腸潰瘍出血は成人に比して稀であるが時に止血困難な症例に遇遭する。止血処置を要した小児期の十二指腸潰瘍出血3例を経験したので報告する。症例1:10歳男児。吸血及び貧血を認め上部消化管内視鏡検査を行い十二指腸球部にDieulafoy潰瘍を認めたためクリッピングにて止血した。症例2:9歳男児。喘息発作に対しステロイド投与を連日行っていたところ、吐下血を認め上部消化管内視鏡検査を行った。十二指腸球部に潰瘍出血を認めたが止血困難であり、interventional radiology(以下IVR)を施行し止血した。症例3:14歳男児。吐下血、出血性ショックを認め上部消化管内視鏡検査を施行した。十二指腸前壁の潰瘍から出血を認め一時止血できたが、入院5日目に再出血し再度内視鏡検査を施行するも止血困難で、IVRにて止血した。小児の十二指腸潰瘍出血に対しても内視鏡治療が第一選択と考えるが、止血困難な場合はIVRも有効な手段である。(著者抄録)
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日本小児外科学会雑誌 54(7) 1347-1350 2018年12月 査読有り症例は10ヵ月、男児。胆汁性嘔吐および経口摂取不良を主訴に来院し、熱源不明の発熱およびイレウスの診断で入院した。翌日の腹部CTにて絞扼による小腸イレウスを疑い、緊急手術を行った。トライツ靱帯から約80cmの部位に捻転した重複腸管を認め先端が炎症性に大網と癒着し、付着部を閉塞起点とした小腸イレウスを来していたため、重複腸管および付着部を含む約20cmの小腸切除を施行した。病理所見では捻転部は腸管粘膜上皮や平滑筋など腸管構造を有し、重複腸管壁は捻転による絞扼で壊死を来していた。膵組織や胃粘膜の迷入は認めなかった。術後にイレウスは順調に改善したが、腹腔内感染による筋膜離開と皮下膿瘍のためドレナージ術を施行し、退院後1ヵ月で腹壁再建術を行った。重複腸管はしばしば遭遇するが本症例は捻転形式が稀で、術前診断が困難であった。今回我々は、まれな重複腸管自体の捻転によりイレウスを発症した1例を経験したので報告する。(著者抄録)
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Pediatric transplantation 22(7) e13239 2018年6月 査読有り
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J Neonatal Perinatal Med, 11 379-385 2018年 査読有り筆頭著者
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Journal of Neonatal Surgery 7(2) 2018年 査読有り筆頭著者
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Journal of Tumor Research & Reports 3(1) 2018年 査読有り筆頭著者
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PEDIATRIC SURGERY INTERNATIONAL 33(6) 713-719 2017年6月 査読有りPurpose Neuroblastoma is a refractory pediatric malignant solid tumor. The previous studies demonstrated that Polyphyllin D, the main constituent of Paris polyphylla, a traditional Chinese medicine, exerts an anti-tumor effect on many tumors. However, its effects against neuroblastomas are unclear. Methods We examined the anti-tumor effect of polyphyllin D in human neuroblastoma using IMR-32 and LA-N-2 cells, which exhibit MYCN gene amplification, and NB-69 cells, which do not exhibit MYCN gene amplification. Results All cell lines showed reduced cell viability in response to polyphyllin D treatment. No caspase-3/-7, -8, and -9 activity was observed in IMR-32 and LA-N-2 cells treated with polyphyllin D. In contrast, activation of caspase-3/-7, and -8 activity was observed in NB-69 cells. When polyphyllin D and specific inhibitors of RIPK3 involved in necroptosis were added to IMR-32 and LA-N-2 cell lines, polyphyllin D-induced cell death was inhibited. Conclusion Together, this indicates that the underlying mechanism of polyphyllin D-induced cell death in NB-69 cells is apoptosis, whereas the cell death of IMR-32 and LA-N-2 cells occurs by necroptosis. We continue research on this topic and look forward the discovery of a new therapeutic agent for neuroblastoma.
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Journal of Pediatric Surgery Case Reports 17 42-45 2017年2月1日 査読有りWe experienced the case of an eight-month-old male with intralobar pulmonary sequestration complicated by gastric duplication. The chief complaint was an abnormal shadow on the chest radiograph the patient was diagnosed with pulmonary sequestration via chest computed tomography (CT). Intraoperative findings and postoperative histopathological examination revealed intralobar pulmonary sequestration with associated gastric duplication. Pulmonary sequestration is relatively rare although there are reports of extralobar pulmonary sequestration with gastric duplication, the present case of intralobar pulmonary sequestration with gastric duplication is extremely rare. This case is considered to be an embryologic bronchopulmonary foregut malformation, and is considered important in understanding the aetiology.
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Journal of Neonatal Surgery 6(2) 2017年 査読有り筆頭著者
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Pediatric transplantation 20(5) 707-710 2016年8月 査読有り
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Case Reports in Clinical Pathology 3(2) 22-25 2016年2月 査読有り
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Journal of Pediatric Surgery Case Reports 3(6) 239-241 2015年5月10日 査読有りGiant ovarian cysts in adolescents are very rare. Those treatment by laparotomy or laparoscopic surgery is discussed with gynecologists and pediatric surgeons because its limited working space and risk of rupture and malignancy. We present a case of minimal laparotomy management of a giant ovarian cystic teratoma in adolescent. A 13-year-old girl presented with abdominal pain and constipation. A CT scan showed a giant simple ovarian cystic tumor in her abdomen measuring 29 x 13 x 24 cm. We made a 3-cm Pfannenstiel incision and inserted an Alexis wound retractor XS. The cyst was completely aspirated without spillage in the intraperitoneal space. In total, 6L of murky brown fluid was aspirated from the cyst. There was no ovarian tissue visible on the cyst wall. The left tube and right ovary and tube were intact. The cyst wall and left ovary tube were dissected free by using a LigaSure. Postoperative recovery was uneventful. Pathological assessment revealed a mature cystic teratoma. The ovarian tissue was included in the part of the cyst wall. We were able to safely perform with minimal laparotomy. Therefore, we consider the for cases of giant ovarian tumors, minimal laparotomy surgery is useful from the safety and cosmetic perspective.
主要なMISC
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Pediatric surgery international 35(6) 723-728 2019年6月PURPOSE: Arctigenin has been shown to have anti-tumor effects in various types of cancers. This study was conducted to verify these effects in the human-derived hepatoblastoma cell line, HUH-6 clone 5 (hereinafter, HUH-6). METHODS: Arctigenin was added to cultured HUH-6 cells, and cellular activity was evaluated by MTS assay. To determine the relationship between reduced cellular activity and apoptosis, we measured the activities of caspase 3/7, 8, and 9 and conducted flow cytometry with Annexin V/PI staining. RESULTS: The MTS assay revealed that cellular activity decreased after arctigenin treatment in a concentration-dependent manner (IC50 = 4 µM). To investigate apoptosis induction, activity assays of caspase 3/7, 8, and 9 were performed. While caspase 3/7 and 8 exhibited high activity, caspase 9 showed no activity. Thus, apoptosis induction may have involved the action of tumor necrosis factor receptor 1 (TNFR1). Flow cytometry conducted with Annexin V/PI staining revealed the occurrence of early apoptosis. CONCLUSION: We found that arctigenin has anti-tumor effects in HUH-6 cells in a concentration-dependent manner. Arctigenin may have exerted its anti-tumor effect by inducing apoptosis via TNFR1, which recruits Complex IIa to activate caspase 8 and 3/7. These results may be useful for developing therapeutic agents for hepatoblastoma.
講演・口頭発表等
9所属学協会
5共同研究・競争的資金等の研究課題
1-
日本学術振興会 科学研究費助成事業 2021年4月 - 2023年3月