医学部
基本情報
経歴
1-
2013年 - 現在
主要な論文
85-
日本小児血液・がん学会雑誌 57(1) 24-27 2020年 査読有り筆頭著者我々は稀な肝未分化肉腫を2例経験したので報告する.症例1は11歳女児.発熱,腹痛で発症し,CTで肝右葉に巨大腫瘍,PET-CTでは腫瘍辺縁に強く集積を認めた.血液検査所見ではLDHとCA125が高値を示し,生検にて肝未分化肉腫と診断した.VAC療法(ビンクリスチン,アクチノマイシンD,シクロフォスファミド)(5クール)後に腫瘍核出術を施行した.術後VAC療法(8クール)を行い治療終了後5年2ヶ月経過し再発なく生存中である.症例2は12歳男児.右上腹部膨隆と腹痛で発症し,CTで肝右葉に腫瘍,PET-CTで腫瘍辺縁に集積を認めた.血液検査所見ではLDHとCA125が高値を示し,肝未分化肉腫と術前診断し右3区域切除術を行った.摘出腫瘍は肝未分化肉腫の病理診断であった.術後VAC療法(5クール)行い治療終了後1年4ヶ月経過し無病生存中である.年長児で特異的腫瘍マーカーの上昇が伴わない肝腫瘍においては,本疾患を想起することが肝要である.(著者抄録)
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J Neonatal Perinatal Med, 11 379-385 2018年 査読有り筆頭著者
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Journal of Neonatal Surgery 7(2) 2018年 査読有り筆頭著者
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Journal of Tumor Research & Reports 3(1) 2018年 査読有り筆頭著者
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PEDIATRIC SURGERY INTERNATIONAL 33(6) 713-719 2017年6月 査読有りPurpose Neuroblastoma is a refractory pediatric malignant solid tumor. The previous studies demonstrated that Polyphyllin D, the main constituent of Paris polyphylla, a traditional Chinese medicine, exerts an anti-tumor effect on many tumors. However, its effects against neuroblastomas are unclear. Methods We examined the anti-tumor effect of polyphyllin D in human neuroblastoma using IMR-32 and LA-N-2 cells, which exhibit MYCN gene amplification, and NB-69 cells, which do not exhibit MYCN gene amplification. Results All cell lines showed reduced cell viability in response to polyphyllin D treatment. No caspase-3/-7, -8, and -9 activity was observed in IMR-32 and LA-N-2 cells treated with polyphyllin D. In contrast, activation of caspase-3/-7, and -8 activity was observed in NB-69 cells. When polyphyllin D and specific inhibitors of RIPK3 involved in necroptosis were added to IMR-32 and LA-N-2 cell lines, polyphyllin D-induced cell death was inhibited. Conclusion Together, this indicates that the underlying mechanism of polyphyllin D-induced cell death in NB-69 cells is apoptosis, whereas the cell death of IMR-32 and LA-N-2 cells occurs by necroptosis. We continue research on this topic and look forward the discovery of a new therapeutic agent for neuroblastoma.
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Journal of Pediatric Surgery Case Reports 17 42-45 2017年2月1日 査読有りWe experienced the case of an eight-month-old male with intralobar pulmonary sequestration complicated by gastric duplication. The chief complaint was an abnormal shadow on the chest radiograph the patient was diagnosed with pulmonary sequestration via chest computed tomography (CT). Intraoperative findings and postoperative histopathological examination revealed intralobar pulmonary sequestration with associated gastric duplication. Pulmonary sequestration is relatively rare although there are reports of extralobar pulmonary sequestration with gastric duplication, the present case of intralobar pulmonary sequestration with gastric duplication is extremely rare. This case is considered to be an embryologic bronchopulmonary foregut malformation, and is considered important in understanding the aetiology.
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Journal of Neonatal Surgery 6(2) 2017年 査読有り筆頭著者
主要なMISC
6-
Pediatric surgery international 35(6) 723-728 2019年6月PURPOSE: Arctigenin has been shown to have anti-tumor effects in various types of cancers. This study was conducted to verify these effects in the human-derived hepatoblastoma cell line, HUH-6 clone 5 (hereinafter, HUH-6). METHODS: Arctigenin was added to cultured HUH-6 cells, and cellular activity was evaluated by MTS assay. To determine the relationship between reduced cellular activity and apoptosis, we measured the activities of caspase 3/7, 8, and 9 and conducted flow cytometry with Annexin V/PI staining. RESULTS: The MTS assay revealed that cellular activity decreased after arctigenin treatment in a concentration-dependent manner (IC50 = 4 µM). To investigate apoptosis induction, activity assays of caspase 3/7, 8, and 9 were performed. While caspase 3/7 and 8 exhibited high activity, caspase 9 showed no activity. Thus, apoptosis induction may have involved the action of tumor necrosis factor receptor 1 (TNFR1). Flow cytometry conducted with Annexin V/PI staining revealed the occurrence of early apoptosis. CONCLUSION: We found that arctigenin has anti-tumor effects in HUH-6 cells in a concentration-dependent manner. Arctigenin may have exerted its anti-tumor effect by inducing apoptosis via TNFR1, which recruits Complex IIa to activate caspase 8 and 3/7. These results may be useful for developing therapeutic agents for hepatoblastoma.
講演・口頭発表等
9所属学協会
5共同研究・競争的資金等の研究課題
1-
日本学術振興会 科学研究費助成事業 2021年4月 - 2023年3月