渡邉俊介

watanabe shunsuke

基本情報

所属: 藤田医科大学医学部医学科小児外科学講師

学位: 博士（医学）(藤田医科大学)

J-GLOBAL ID: 201501009281932970
researchmap会員ID: 7000012895

研究キーワード

研究分野

ライフサイエンス / 外科学一般、小児外科学 / 小児外科

経歴

2013年 - 現在

藤田医科大学小児外科

主要な論文

長期生存が期待される小児肝未分化肉腫の2例

渡邉俊介, 土屋智寛, 近藤靖浩, 直江篤樹, 宇賀菜緒子, 安井稔博, 原普二夫, 鈴木達也

日本小児血液・がん学会雑誌 57(1) 24-27 2020年査読有り筆頭著者

我々は稀な肝未分化肉腫を2例経験したので報告する.症例1は11歳女児.発熱,腹痛で発症し,CTで肝右葉に巨大腫瘍,PET-CTでは腫瘍辺縁に強く集積を認めた.血液検査所見ではLDHとCA125が高値を示し,生検にて肝未分化肉腫と診断した.VAC療法(ビンクリスチン,アクチノマイシンD,シクロフォスファミド)(5クール)後に腫瘍核出術を施行した.術後VAC療法(8クール)を行い治療終了後5年2ヶ月経過し再発なく生存中である.症例2は12歳男児.右上腹部膨隆と腹痛で発症し,CTで肝右葉に腫瘍,PET-CTで腫瘍辺縁に集積を認めた.血液検査所見ではLDHとCA125が高値を示し,肝未分化肉腫と術前診断し右3区域切除術を行った.摘出腫瘍は肝未分化肉腫の病理診断であった.術後VAC療法(5クール)行い治療終了後1年4ヶ月経過し無病生存中である.年長児で特異的腫瘍マーカーの上昇が伴わない肝腫瘍においては,本疾患を想起することが肝要である.(著者抄録)
Effects of assisted reproductive technologies in neonates with indications for surgery (2007-2016)

Shunsuke Watanabe, Yasuhiro Kondo, Atuki Naoe, Toshihiro Yasui, Tatsuya Suzuki, Fujio Ha-ra, Naoko Uga, Masafumi Miyata, Hiroko Boda

J Neonatal Perinatal Med, 11 379-385 2018年査読有り筆頭著者
Efficacy of Peritoneal Drainage for Focal Intestinal Perforation.

Shunsuke Watanabe, Tatsuya Suzuki, Yasuhiro Kondo, Atuki Naoe, Naoko Uga, Toshihiro Yasui, Fujio Hara, Masafumi Miyata, Hiroko Boda, Tetsushi Yoshikawa

Journal of Neonatal Surgery 7(2) 2018年査読有り筆頭著者
A Case of Retroperitoneal Primary Neuroblastoma that Developed in Adulthood

Shunsuke Watanabe, Fujio Hara, Yasuhiro Kondo, Tatsuya Suzuki, Toshihiro Yasui, Naoko Uga, Atuki Naoe

Journal of Tumor Research & Reports 3(1) 2018年査読有り筆頭著者
Polyphyllin D, a steroidal saponin in Paris polyphylla, induces apoptosis and necroptosis cell death of neuroblastoma cells

Shunsuke Watanabe, Tatuya Suzuki, Fujio Hara, Toshihiro Yasui, Naoko Uga, Atuki Naoe

PEDIATRIC SURGERY INTERNATIONAL 33(6) 713-719 2017年6月査読有り

Purpose Neuroblastoma is a refractory pediatric malignant solid tumor. The previous studies demonstrated that Polyphyllin D, the main constituent of Paris polyphylla, a traditional Chinese medicine, exerts an anti-tumor effect on many tumors. However, its effects against neuroblastomas are unclear. Methods We examined the anti-tumor effect of polyphyllin D in human neuroblastoma using IMR-32 and LA-N-2 cells, which exhibit MYCN gene amplification, and NB-69 cells, which do not exhibit MYCN gene amplification. Results All cell lines showed reduced cell viability in response to polyphyllin D treatment. No caspase-3/-7, -8, and -9 activity was observed in IMR-32 and LA-N-2 cells treated with polyphyllin D. In contrast, activation of caspase-3/-7, and -8 activity was observed in NB-69 cells. When polyphyllin D and specific inhibitors of RIPK3 involved in necroptosis were added to IMR-32 and LA-N-2 cell lines, polyphyllin D-induced cell death was inhibited. Conclusion Together, this indicates that the underlying mechanism of polyphyllin D-induced cell death in NB-69 cells is apoptosis, whereas the cell death of IMR-32 and LA-N-2 cells occurs by necroptosis. We continue research on this topic and look forward the discovery of a new therapeutic agent for neuroblastoma.
Intralobar pulmonary sequestration complicated by gastric duplication

Shunsuke Watanabe, Tatuya Suzuki, Fujio Hara, Toshihiro Yasui, Naoko Uga, Atuki Naoe

Journal of Pediatric Surgery Case Reports 17 42-45 2017年2月1日査読有り

We experienced the case of an eight-month-old male with intralobar pulmonary sequestration complicated by gastric duplication. The chief complaint was an abnormal shadow on the chest radiograph the patient was diagnosed with pulmonary sequestration via chest computed tomography (CT). Intraoperative findings and postoperative histopathological examination revealed intralobar pulmonary sequestration with associated gastric duplication. Pulmonary sequestration is relatively rare although there are reports of extralobar pulmonary sequestration with gastric duplication, the present case of intralobar pulmonary sequestration with gastric duplication is extremely rare. This case is considered to be an embryologic bronchopulmonary foregut malformation, and is considered important in understanding the aetiology.
Omphalocele and Gastroschisis in Newborns: Over 16 Years of Experience from a Single Clinic.

Shunsuke Watanabe, Tatuya Suzuki, Fujio Hara, Toshihiro Yasui, Naoko Uga, Atuki Naoe

Journal of Neonatal Surgery 6(2) 2017年査読有り筆頭著者

もっとみる

主要なMISC

Arctigenin induces apoptosis in human hepatoblastoma cells.

Atsuki Naoe, Tomonori Tsuchiya, Yasuhiro Kondo, Naoko Uga, Shunsuke Watanabe, Toshihiro Yasui, Fujio Hara, Tatsuya Suzuki

Pediatric surgery international 35(6) 723-728 2019年6月

PURPOSE: Arctigenin has been shown to have anti-tumor effects in various types of cancers. This study was conducted to verify these effects in the human-derived hepatoblastoma cell line, HUH-6 clone 5 (hereinafter, HUH-6). METHODS: Arctigenin was added to cultured HUH-6 cells, and cellular activity was evaluated by MTS assay. To determine the relationship between reduced cellular activity and apoptosis, we measured the activities of caspase 3/7, 8, and 9 and conducted flow cytometry with Annexin V/PI staining. RESULTS: The MTS assay revealed that cellular activity decreased after arctigenin treatment in a concentration-dependent manner (IC50 = 4 µM). To investigate apoptosis induction, activity assays of caspase 3/7, 8, and 9 were performed. While caspase 3/7 and 8 exhibited high activity, caspase 9 showed no activity. Thus, apoptosis induction may have involved the action of tumor necrosis factor receptor 1 (TNFR1). Flow cytometry conducted with Annexin V/PI staining revealed the occurrence of early apoptosis. CONCLUSION: We found that arctigenin has anti-tumor effects in HUH-6 cells in a concentration-dependent manner. Arctigenin may have exerted its anti-tumor effect by inducing apoptosis via TNFR1, which recruits Complex IIa to activate caspase 8 and 3/7. These results may be useful for developing therapeutic agents for hepatoblastoma.
Evaluation of Ki-67 as prognostic factor for pediatric neuroblastoma and the possibility of molecular-targeted drugs with vascular endothelial growth factor and platelet-derived growth factor receptor.Evaluation of Ki-67 as prognostic factor for pediatric neuroblastoma and the possibility of molecular-targeted drugs with vascular endothelial growth factor and platelet-derived growth factor receptor.

Minerva Pediatrica. 2019年1月2日

もっとみる

講演・口頭発表等

小児漏斗胸に対する胸骨挙上術 ―内胸動脈温存の試み―

渡邉俊介, 土屋智寛, 近藤靖浩, 直江篤樹, 宇賀菜緒子, 安井稔博, 原普二夫, 鈴木達也

第56回日本小児外科学会, 2019 2019年5月
Eight cases of perineal canal

2018年5月
ヒト神経芽細胞種培養細胞株に対するPolyphyllinの抗腫瘍効果に関する研究

渡邉俊介, 宇賀菜緒子, 直江篤樹, 安井稔博, 原普二夫, 鈴木達也

第53回日本小児外科学会 2016年5月23日
気管無形性の1例

渡邉俊介, 宇賀菜緒子, 直江篤樹, 安井稔博, 原普二夫, 鈴木達也

第49回東海小児外科学会東海地方会 2015年12月
学童期に発症した右膝軟部明細胞肉腫の一例

渡邉俊介, 宇賀菜緒子, 直江篤樹, 安井, 稔博, 原普二夫, 鈴木達也

第52回日本小児外科学会 2015年5月29日兵庫