研究者業績

辻 雄大

ツジ ユウダイ  (Yudai Tsuji)

基本情報

所属
藤田医科大学 医学部 生体構造学(旧 解剖学Ⅱ) 助教
学位
博士(理学)(同志社大学)

ORCID ID
 https://orcid.org/0009-0006-7462-2234
J-GLOBAL ID
202101014437516876
researchmap会員ID
R000022318

論文

 1
  • Yudai Tsuji, Shinichi Yamaguchi, Tomoyuki Nakamura, Masaya Ikegawa
    Applied Sciences 11(22) 11038-11038 2021年11月22日  査読有り筆頭著者
    Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is increasingly used in a broad range of research due to its ability to visualize the spatial distribution of metabolites in vivo. Here, we have developed a method, named thoracic Mass Spectrometry Imaging (tMSI), as a standard protocol of molecular imaging of whole-animal sectioning in various settings of mice in vivo. Further application of the strategy that involved the systemic administration of dexamethasone (DEX) in mice, enabled a dynamic shift in the energy status of multiple thoracic organs to be visualized, based on tMSI data of purine and pyrimidine metabolites. Furthermore, with the introduction of uniform manifold approximation and projection (UMAP) for tMSI data, metabolic profiles normally localized in the cortex and cortico-medullary junction (CMJ) of the thymus were drastically shifted as minor profiles into the medulla of DEX-treated thymus. As a massive apoptotic cell death in the thymic cortex was noticeable, a single molecule, which was upregulated in the cortex of the thymus, enabled us to predict ongoing immunosuppression by in vivo DEX-administration.

MISC

 10

講演・口頭発表等

 30

担当経験のある科目(授業)

 1

共同研究・競争的資金等の研究課題

 3

産業財産権

 4