研究者業績

刑部 恵介

オサカベ ケイスケ  (Keisuke Osakabe)

基本情報

所属
藤田医科大学 医療科学部 臨床検査学科 臨床生理・画像情報解析学領域 准教授
学位
博士(医学)

J-GLOBAL ID
200901091924557501
researchmap会員ID
1000205084

論文

 69
  • Keisuke Maeda, Nami Hosoda, Himari Tsuboi, Honoka Naito, Chiaki Kudo, Junichi Fukumoto, Shiho Fujita, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Shunta Yamaguchi, Naoko Ishihara
    Epilepsia open 2024年12月3日  
    OBJECTIVE: Epilepsy treatment with anti-seizure medications (ASMs) is based on careful assessment of the balance between the likelihood of further seizures and the risk of side effects of treatment. However, there is currently no established biomarker to ascertain seizure control status with ASMs. High-frequency oscillations (HFOs), transient bursts of EEG activity with frequencies beyond 80 Hz, are a new and promising noninvasive epilepsy biomarker. We compared the risk of scalp HFO appearance between pediatric patients with good and poor seizure control by treatment with ASMs. METHODS: A total of 72 epilepsy patients (aged 0-18 years, 39 males) with good and poor seizure control with ASMs participated in this study. We applied a validated automated detector to determine HFO and spike. We calculated the odds ratios (ORs) for scalp HFO and spike appearance according to seizure control status by multiple logistic regression analysis. RESULTS: Scalp HFO was seen more commonly and with a significantly higher detection rate in patients with poor seizure control as compared with patients with good seizure control for both ripple and fast ripple. These significant associations were found for both focal and generalized epilepsy. The ORs for scalp HFO appearance adjusted for confounding factors were significantly higher in patients with poor seizure control compared to those with good seizure control (ripple: OR [95% CI] = 11.91 [2.21-64.30], p = 0.004; fast ripple: 4.98 [1.03-24.09], p = 0.046). There were no significant associations between spike appearance and seizure control status. SIGNIFICANCE: We found an increased risk of scalp HFO appearance in patients with poor seizure control. The results of this study support that scalp HFO is associated with patients having frequent seizures after treatment in both ripple and fast ripple. PLAIN LANGUAGE SUMMARY: This study analyzed scalp high-frequency oscillations and spikes in pediatric patients with various types of epilepsy who were being treated using ASMs. The results showed that an increased risk of scalp HFO appearance was observed in patients with poor seizure control compared to those with good seizure control. These findings were observed in both the ripple (80-250 Hz) and fast ripple (250-500 Hz) bands. The scalp HFO is associated with patients having frequent seizures after treatment in both ripple and fast ripple.
  • Keisuke Maeda, Nami Hosoda, Junichi Fukumoto, Shun Kawai, Mizuki Hayafuji, Himari Tsuboi, Shiho Fujita, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Naoko Ishihara
    Journal of clinical neurophysiology : official publication of the American Electroencephalographic Society 2023年10月30日  
    INTRODUCTION: High-frequency oscillation (HFO) in scalp electroencephalography is a promising new noninvasive prognostic epilepsy biomarker, but further data are needed to ascertain the utility of this parameter. The present work investigated the association between epileptic activity and scalp HFO in pediatric patients with various types of epilepsy, using multivariable regression models to correct for possible confounding factors. METHODS: The authors analyzed 97 subjects who were divided into groups with active epilepsy (within 1 year of seizure), seizure-free epilepsy (>1 year without seizure), and nonepilepsy. Regarding the frequency of seizure occurrence as an indicator of epileptic activity, we categorized subjects into four groups (Daily/Weekly, Monthly, Yearly, and Rarely). RESULTS: Multiple linear regression analysis showed that the scalp HFO detection rate was significantly higher in patients with active epilepsy than in those with nonepilepsy (β [95% confidence interval] = 2.77 [1.79-4.29]; P < 0.001). The association between scalp HFO detection rate and frequency of seizure occurrence was highest in the Daily/Weekly group (β [95% confidence interval] = 3.38 [1.57-7.27]; P = 0.002), followed by Monthly and Yearly groups (β [95% confidence interval] = 2.42 [1.02-5.73]; P = 0.046 and 0.36 [0.16-0.83]; P = 0.017). In addition, HFO duration, number of peaks, and number of channels detected were significantly higher in patients with active epilepsy. CONCLUSIONS: Pediatric patients with active epilepsy and high frequency of seizure occurrence exhibited a higher scalp HFO detection rate. These results may help to establish HFO detectable by noninvasive scalp electroencephalography as a biomarker of active epilepsy in pediatric patients.
  • Yuji Hattori, Hiroya Yamada, Eiji Munetsuna, Ryosuke Fujii, Yoshitaka Ando, Mirai Yamazaki, Genki Mizuno, Yoshiki Tsuboi, Yuya Ishihara, Naohiro Ichino, Keiko Sugimoto, Keisuke Osakabe, Hiroaki Ishikawa, Koji Ohashi, Koji Suzuki
    Genetic testing and molecular biomarkers 27(8) 239-247 2023年8月  
    Background: The increasing prevalence of non-alcoholic fatty liver disease (NAFLD) has become a global health problem. NAFLD has few initial symptoms and may be difficult to detect early, so there is need for a minimally invasive early detection marker. We hypothesized that miR-122 and miR-20a levels combined, as the miR-122/miR-20a ratio might detect NAFLD more sensitively. Methods: This study involved 167 participants with low alcohol intake. Those who had an increase in echogenicity of the liver parenchyma and hepato-renal contrast on ultrasonography were classified as the NAFLD group (n = 44), which was further classified into mild (n = 26) and severe (n = 18) groups based on echogenic intensity and hepatic vessel and diaphragm visualization. Participants without fatty liver were included in the normal group, except for those with an abnormal body mass index, glycated hemoglobin, and systolic blood pressure (n = 123) values. Serum miR-122 and miR-20a expression levels in participants were measured by real-time polymerase chain reaction, and the miR-122/miR-20a was calculated. Results: In the NAFLD group, miR-122 expression was significantly higher and the miR-20a was significantly lower than in the normal group, in agreement with previous studies. miR-122/miR-20a was also significantly higher in the NAFLD group. Receiver operating characteristic curve analysis was performed with miR-122/miR-20a as an NAFLD detection marker, and the area under the curve of miR-122/miR-20a was significantly larger than that of miR-122 or miR-20a alone. Conclusions: The miR-122/miR-20a ratio, combined with miR-122 and miR-20a levels, is a useful biomarker to detect NAFLD with high sensitivity.
  • 川部 直人, 刑部 恵介, 杉山 博子, 市野 直浩, 田中 浩敬, 中岡 和徳, 大野 栄三郎, 葛谷 貞二, 橋本 千樹, 廣岡 芳樹
    超音波医学 50(Suppl.) S218-S218 2023年4月  
  • 刑部 恵介, 市野 直浩, 杉山 博子, 笹木 優賢, 安井 駿豊, 佐野 友亮, 葛谷 貞二, 川部 直人, 橋本 千樹, 廣岡 芳樹
    超音波医学 50(Suppl.) S237-S237 2023年4月  
  • 川部 直人, 刑部 恵介, 杉山 博子, 市野 直浩, 田中 浩敬, 中岡 和徳, 大野 栄三郎, 葛谷 貞二, 橋本 千樹, 廣岡 芳樹
    超音波医学 50(Suppl.) S218-S218 2023年4月  
  • 刑部 恵介, 市野 直浩, 杉山 博子, 笹木 優賢, 安井 駿豊, 佐野 友亮, 葛谷 貞二, 川部 直人, 橋本 千樹, 廣岡 芳樹
    超音波医学 50(Suppl.) S237-S237 2023年4月  
  • Genki Mizuno, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Yoshitaka Ando, Ryosuke Fujii, Yoshiki Tsuboi, Atsushi Teshigawara, Itsuki Kageyama, Keisuke Osakabe, Keiko Sugimoto, Hiroaki Ishikawa, Naohiro Ichino, Yoshiji Ohta, Koji Ohashi, Shuji Hashimoto, Koji Suzuki
    Endocrine Research 47(3-4) 130-137 2022年10月2日  
  • 鈴木 雅大, 刑部 恵介, 杉山 博子, 朝田 和佳奈, 安井 駿豊, 小林 晟奈, 葛谷 貞二, 川部 直人, 橋本 千樹, 廣岡 芳樹
    超音波医学 49(Suppl.) S246-S246 2022年4月  
  • 杉山 博子, 刑部 恵介, 鈴木 雅大, 安井 駿豊, 小林 晟奈, 光岡 大貴, 葛谷 貞二, 川部 直人, 橋本 千樹, 廣岡 芳樹
    超音波医学 49(Suppl.) S494-S494 2022年4月  
  • 津田 恵里花, 杉本 恵子, 杉本 邦彦, 前田 圭介, 刑部 恵介, 市野 直浩, 山田 晶, 鈴木 康司
    超音波医学 49(Suppl.) S698-S698 2022年4月  
  • 藤田 優, 杉本 恵子, 刑部 恵介, 高井 洋次, 前田 圭介, 近藤 百華, 津田 恵里花, 鈴木 康司, 市野 直浩
    超音波医学 49(Suppl.) S790-S790 2022年4月  
  • Yuji Hattori, Hiroya Yamada, Eiji Munetsuna, Yoshitaka Ando, Genki Mizuno, Ryosuke Fujii, Yoshiki Tsuboi, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Hiroaki Ishikawa, Koji Ohashi, Koji Suzuki
    Endocrine journal 69(8) 999-1006 2022年3月31日  
    The increasing prevalence of nonalcoholic fatty liver disease (NAFLD) is a global health problem. In recent years, the inhibitory effect of brain-derived neurotrophic factor (BDNF) on diabetes mellitus and fatty liver has been clarified. The purpose of this study was to analyze the relationship between serum BDNF and NAFLD which caused by abnormal metabolism of glucose and lipids. This cross-sectional study involved 429 participants (mean age, 63.5 years: men, 38.5%) with low alcohol intake. Of the participants, those who had an increase in echogenicity of the liver parenchyma and hepato-renal contrast on ultrasonography were classified as the NAFLD group (n = 88), and the others were classified as the normal (n = 341) group. The NAFLD group was further classified into a mild group (n = 60) and a severe group (n = 28) based on the intensity of echogenicity and visualization of the hepatic vessels and diaphragm. Median BDNF levels were higher in the NAFLD group than the normal group (35.5 vs. 42.3 ng/mL, p < 0.01). Furthermore, BDNF levels tended to be associated with the severity of NAFLD (p < 0.01). In addition to the univariate analysis, in the sex- and age-adjusted model, there was a significant association between the BDNF levels and NAFLD severity (p < 0.01). The fully adjusted regression analysis also showed a positive association between the serum BDNF level and NAFLD (p < 0.01). These results suggest that NAFLD patients have a compensatory increase in circulating BDNF levels.
  • 川部 直人, 橋本 千樹, 葛谷 貞二, 中野 卓二, 中岡 和徳, 杉山 博子, 西川 徹, 刑部 恵介, 市野 直浩, 廣岡 芳樹
    超音波医学 48(Suppl.) S260-S260 2021年4月  
  • 川部 直人, 橋本 千樹, 葛谷 貞二, 中野 卓二, 中岡 和徳, 杉山 博子, 西川 徹, 刑部 恵介, 市野 直浩, 廣岡 芳樹
    超音波医学 48(Suppl.) S260-S260 2021年4月  
  • 刑部 恵介, 市野 直浩, 杉山 博子, 朝田 和佳奈, 鈴木 雅大, 西川 徹, 近藤 百華, 川部 直人, 橋本 千樹, 廣岡 芳樹
    超音波医学 48(Suppl.) S274-S274 2021年4月  
  • 高井 洋次, 刑部 恵介, 杉本 恵子, 鈴木 康司, 市野 直浩
    超音波医学 48(Suppl.) S450-S450 2021年4月  
  • Ando Y, Yamazaki M, Yamada H, Munetsuna E, Fujii R, Mizuno G, Ichino N, Osakabe K, Sugimoto K, Ishikawa H, Ohashi K, Teradaira R, Ohta Y, Hamajima N, Hashimoto S, Suzuki K
    Scientific reports 9(1) 18856-18856 2019年12月  査読有り
  • 刑部 恵介, 市野 直浩, 杉山 博子, 朝田 和佳奈, 鈴木 雅大, 安井 駿豊, 川部 直人, 橋本 千樹, 廣岡 芳樹, 吉岡 健太郎
    Kameraden (69) 6-6 2019年10月  
  • Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Mirai Yamazaki, Yoshiki Tsuboi, Mari Kondo, Genki Mizuno, Hiroaki Ishikawa, Keiko Sugimoto, Keisuke Osakabe, Naohiro Ichino, Koji Ohashi, Nobuyuki Hamajima, Koji Suzuki
    Annals of clinical biochemistry 55(4) 437-445 2018年7月  査読有り
    Purpose It has been demonstrated that circulating microRNA profiles are affected by physiological conditions. Several studies have demonstrated that microRNAs play important roles in the regulation of adiposity. However, few have investigated the relationship between circulating microRNAs and obesity, which has become a major public health problem worldwide. This study investigated the association between circulating microRNAs and obesity in a Japanese population. Methods Obesity parameters, such as subcutaneous and visceral fat adipose tissue, body fat percentage, and body mass index were assessed in a cross-sectional sample of 526 participants who attended health examinations in Yakumo, Japan. In addition, five circulating microRNAs (miR-20a, -21, -27a, -103a, and -320), which are involved in adipocyte proliferation and differentiation, were quantified using real-time polymerase chain reaction amplification. Results We compared the circulating microRNA concentrations in a percentile greater than 75th (high) with below the value (low) of subcutaneous adipose tissue, visceral fat adipose tissue, body mass index, and per cent body fat. For visceral fat adipose tissue, significant decrease in miR-320 expression was observed in high group. Also, for body mass index, significant change of miR-20a, -27a, 103a, and 320 expression level was observed in high group. Multiple linear regression analysis demonstrated that circulating levels of some microRNA such as miR-27a were significantly associated with subcutaneous adipose tissue, visceral fat adipose tissue, and body mass index. Conclusions Our findings support the need for further studies to determine whether such changes are consistent across different populations and whether the identified microRNAs may represent novel biomarkers to predict the susceptibility and progression of obesity-related disorders.
  • Ryosuke Fujii, Jun Ueyama, Arisa Aoi, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Koji Suzuki, Nobuyuki Hamajima, Kenji Wakai, Takaaki Kondo
    Environmental Health and Preventive Medicine 23(1) 1 2018年1月2日  査読有り
    Background: The effect of the redox state of human serum albumin (HSA) on the antioxidant properties of the entire body has been a focus of recent research. The usefulness of HSA redox state as a biomarker for reducing oxidative stress has been investigated in clinical settings however, evidence for its significance as a health index in non-clinical settings is yet to be established. This study aimed to examine the associations between HSA redox state and the atherosclerotic indices of carotid intima-media thickness (IMT) and plaque formation in a rural Japanese population. Methods: We conducted a cross-sectional study as part of a health check-up program in the rural area of Hokkaido, Japan, at the end of August 2013. A total of 281 residents (124 men and 157 women) were included in the final analysis. Lifestyle-related data were obtained through a self-reported questionnaire, and ultrasound examinations were performed to measure IMT and determine plaque formation. The high-performance liquid chromatography postcolumn bromocresol green method was used to separate HSA into human nonmercaptalbumin and human mercaptalbumin (HMA). Results: We found a significant negative relationship between the fraction of HMA [f(HMA)] and IMT (standardized β = - 0.132, p = 0.03). Moreover, f(HMA) was significantly associated with plaque formation (p &lt 0.01) with an odds ratio of 0.89 (95% confidence interval, 0.81-0.97) for every 10% increment in f(HMA). Conclusions: We found that the HSA redox state, as determined by f(HMA), was associated with atherosclerotic indices in Japanese subjects. These results suggest that the HSA redox state indicates the risk of developing atherosclerosis.
  • Yuka Ochi, Senju Hashimoto, Naoto Kawabe, Michihito Murao, Takuji Nakano, Toshiki Kan, Kazunori Nakaoka, Masashi Ohki, Takamitsu Kurashita, Tomoki Takamura, Sayuri Nomura, Toru Nishikawa, Aiko Fukui, Keisuke Osakabe, Naohiro Ichino, Kentaro Yoshioka
    HEPATOLOGY RESEARCH 47(8) 755-766 2017年7月  査読有り
    AimGenome-wide association studies have revealed that single nucleotide polymorphism (SNP) of human leukocyte antigen (HLA)-DQ is associated with the clearance of hepatitis B surface antigen (HBsAg) in acute hepatitis B virus (HBV) infection. We examined the effects of SNPs on the development of hepatocellular carcinoma (HCC) and markers of HBV in chronic HBV infection. MethodsThe SNPs of HLA-DQ (rs2856718 and rs7453920) were determined in 299 patients with chronic HBV infection. ResultsIn 224 hepatitis B e antigen (HBeAg)-negative patients, those with rs2856718 genotype AG+GG had significantly lower hepatitis B core-related antigen levels (P=0.0184), less frequent treatment with nucleotide/nucleoside analogs (NAs) (P=0.0433), and less frequent HCC development (P=0.0256) than those with genotype AA. Multivariate analysis selected age (P=0.0460), platelet count (P=0.0481), -glutamyl transpeptidase (P=0.0030), and nucleotide/nucleoside analog treatment (P=0.0003) as factors independently associated with HCC development. HBeAg-negative patients with rs7453920 genotype GG had significantly lower HBsAg levels (P&lt;0.0001), a higher prevalence of HBV genotype C (P=0.0063), and a lower prevalence of the wild-type basal core promoter region (P=0.0045) than those with genotype AA + AG. Multivariate analysis selected age (P&lt;0.0001), platelet count (P=0.0021), HBV DNA levels (P=0.0314), wild type of precore region (P=0.0015), and rs7453920 (P&lt;0.0001) as factors independently associated with HBsAg levels. ConclusionThis study revealed an association between rs2856718 and HCC development and an association between rs7453920 and HBsAg levels.
  • Ochi Yuka, Naoto Kawabe, Senju Hashimoto, Michihito Murao, Takuji Nakano, Toshiki Kan, Kazunori Nakaoka, Masashi Ohki, Takamitsu Kurashita, Tomoki Takamura, Sayuri Nomura, Toru Nishikawa, Keisuke Osakabe, Naohiro Ichino, Aiko Fukui, Kentaro Yoshioka
    HEPATOLOGY 64 900A-900A 2016年10月  査読有り
  • Naoto Kawabe, Toshiki Kan, Tomoki Takamura, Sayuri Nomura, Senju Hashimoto, Michihito Murao, Takuji Nakano, Kazunori Nakaoka, Masashi Ohki, Ochi Yuka, Takamitsu Kurashita, Aiko Fukui, Toru Nishikawa, Keisuke Osakabe, Naohiro Ichino, Kentaro Yoshioka
    HEPATOLOGY 64 462A-462A 2016年10月  査読有り
  • Kentaro Yoshioka, Ochi Yuka, Senju Hashimoto, Naoto Kawabe, Michihito Murao, Takuji Nakano, Toshiki Kan, Kazunori Nakaoka, Masashi Ohki, Takamitsu Kurashita, Tomoki Takamura, Sayuri Nomura, Toru Nishikawa, Keisuke Osakabe, Naohiro Ichino
    HEPATOLOGY 64 894A-894A 2016年10月  査読有り
  • Koji Suzuki, Hiroya Yamada, Ayuri Nagura, Koji Ohashi, Hiroaki Ishikawa, Mirai Yamazaki, Yoshitaka Ando, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Nobuyuki Hamajima, Takashi Inoue
    Fujita Medical Journal 2(1) 1-5 2016年  査読有り
    Objectives: Circulating micro (mi)RNA profiles are influenced by various medical conditions, and miRNAs have been examined as potential biomarkers for cancer, cardiovascular disease, and diabetes. However, few studieshave investigated whether circulating miRNAs and cigarette smoking are correlated. Our aim was to determine theassociation between smoking status and expression of various miRNAs in a Japanese population sample.Methods: We conducted a cross-sectional analysis of 526 subjects (219 men and 307 women) aged 39 years and older who had undergone a health examination at a clinic in Yakumo, Hokkaido in August 2012. We used quantitative real-time polymerase chain reaction to determine serum miRNA expression. We then calculated the odds ratios for elevated serum miRNA levels according to smoking status using never-smokers as the reference group.Results: Expression of lethal (let)-7d, miRNA(miR)-150, miR-192, miR-197 and miR320 was significantly higher in current smokers than in never-smokers. Multivariate logistic regression analyses showed that while current smokers were significantly more likely to have elevated serum levels of miRNA let-7d, miR-21, miR-122, miR-146, miR-150, miR-192, miR-197, and miR320 than never-smokers, former smokers had significantly higher odds of having elevated miR-1, miR-146, miR-150, miR-195, and miR-320 levels in their sera.Conclusions: We found that cigarette smoking is associated with elevated expression of various serum miRNAs. Our results suggest that it is necessary to consider the confounding effect caused by smoking when evaluating expression of serum miRNAs for diagnosing pathological conditions.
  • Keisuke Osakabe, Naohiro Ichino, Toru Nishikawa, Hiroko Sugiyama, Miho Kato, Ai Shibata, Wakana Asada, Naoto Kawabe, Senju Hashimoto, Michihito Murao, Takuji Nakano, Hiroaki Shimazaki, Toshiki Kan, Kazunori Nakaoka, Yuka Takagawa, Masashi Ohki, Takamitsu Kurashita, Tomoki Takamura, Kentaro Yoshioka
    WORLD JOURNAL OF GASTROENTEROLOGY 21(35) 10215-10223 2015年9月  査読有り
    AIM: To evaluate the changes of shear-wave velocity (Vs) by acoustic radiation force impulse after treatment in chronic hepatitis C. METHODS: Eighty-seven patients with chronic hepatitis C were consecutively treated with combinations of interferon (IFN) plus ribavirin (RBV). Vs value (m/s) was measured with acoustic radiation force impulse before treatment, at end of treatment (EOT), 1 year after EOT, and 2 years after EOT. RESULTS: In patients with a sustained virological response (SVR) (n = 41), Vs significantly decreased at EOT [1.19 (1.07-1.37), P = 0.0004], 1 year after EOT [1.10 (1.00-1.22), P = 0.0001], and 2 years after EOT [1.05 (0.95-1.16), P &lt; 0.0001] compared with baseline [1.27 (1.11-1.49)]. In patients with a relapse (n = 26), Vs did not significantly decrease at EOT [1.23 (1.12-1.55)], 1 year after EOT [1.20 (1.12-1.80)], and 2 years after EOT [1.41 (1.08-2.01)] compared with baseline [1.39 (1.15-1.57)]. In patients with a nonvirological response (n = 20), Vs did not significantly decrease at EOT [1.64 (1.43-2.06)], 1 year after EOT [1.66 (1.30-1.95)], and 2 years after EOT [1.61 (1.36-2.37)] compared with baseline [1.80 (1.54-2.01)]. Among genotype 1 patients, baseline Vs was significantly lower in SVR patients [1.28 (1.04-1.40)] than in non-SVR patients [1.56 (1.20-1.83)] (P = 0.0142). CONCLUSION: Reduction of Vs values was shown in SVR patients after IFN-plus-RBV therapy by acoustic radiation force impulse.
  • Kazunori Nakaoka, Senju Hashimoto, Naoto Kawabe, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Hiroaki Shimazaki, Toshiki Kan, Yuka Takagawa, Masashi Ohki, Takamitsu Kurashita, Tomoki Takamura, Toru Nishikawa, Naohiro Ichino, Keisuke Osakabe, Kentaro Yoshioka
    SPRINGERPLUS 4 2015年2月  査読有り
    Aim: To investigate associations between patatin-like phospholipase domain-containing 3 (PNPLA3) genotypes and fibrosis and hepatocarcinogenesis in Japanese chronic hepatitis C (CHC) patients. Methods: Two hundred and thirty-one patients with CHC were examined for PNPLA3 genotypes, liver stiffness measurements (LSM), and hepatocellular carcinoma (HCC) from May 2010 to October 2012 at Fujita Health University Hospital. The rs738409 single nucleotide polymorphism (SNP) encoding for a functional PNPLA3 I148M protein variant was genotyped using a TaqMan predesigned SNP genotyping assay. LSM was determined as the velocity of a shear wave (Vs) with an acoustic radiation force impulse. Vs cut-off values for cirrhosis were set at 1.55 m/s. We excluded CHC patients with a sustained virological response or relapse after interferon treatment. Results: PNPLA3 genotypes were CC, CG, and GG for 118, 72, and 41 patients, respectively. Multivariable logistic regression analysis selected older age (OR = 1.06; 95% CI: 1.03-1.09; p &lt; 0.0001), higher body mass index (BMI) (OR= 1.12; 95% CI: 1.03-1.22; p = 0.0082), and PNPLA3 genotype GG (OR = 2.07; 95% CI: 0.97-4.42; p = 0.0599) as the factors independently associated with cirrhosis. When 137 patients without past history of interferon treatment were separately assessed, multivariable logistic regression analysis selected older age (OR = 1.05; 95% CI: 1.02-1.09; p = 0.0034), and PNPLA3 genotype GG (OR = 3.35; 95% CI: 1.13-9.91; p = 0.0291) as the factors independently associated with cirrhosis. Multivariable logistic regression analysis selected older age (OR = 1.12; 95% CI: 1.07-1.17; p &lt; 0.0001), PNPLA3 genotype GG (OR = 2.62; 95% CI: 1.15-5.96; p = 0.0218), and male gender (OR = 1.83; 95% CI: 0.90-3.71); p = 0.0936) as the factors independently associated with HCC. Conclusion: PNPLA3 genotype I148M is one of risk factors for developing HCC in Japanese CHC patients, and is one of risk factors for progress to cirrhosis in the patients without past history of interferon treatment.
  • 加藤 美穂, 西川 徹, 杉山 博子, 青山 和佳奈, 柴田 亜委, 久保 仁美, 豆谷 果奈, 杉本 邦彦, 石川 隆志, 石井 潤一, 刑部 恵介, 市野 直浩
    超音波検査技術抄録集 40 S123-S123 2015年  
  • Ichino N, Osakabe K, Sugimoto K, Suzuki K, Yamada H, Takai H, Sugiyama H, Yukitake J, Inoue T, Ohashi K, Hata T, Hamajima N, Nishikawa T, Hashimoto S, Kawabe N, Yoshioka K
    Rinsho Byori 63(1) 32-43 2015年1月  査読有り
  • Rika Watarai, Koji Suzuki, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Hiroya Yamada, Takeshi Hamajima, Nobuyuki Hamajima, Takashi Inoue
    JOURNAL OF EPIDEMIOLOGY 24(3) 250-257 2014年5月  査読有り
    Background: Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of endothelium nitric oxide synthase (NOS). ADMA binds to a substrate-binding site of NOS and then inhibits nitric oxide production from vascular endothelial cells. Elevated ADMA levels are a risk factor for cardiovascular disease. Recently, it was reported that plasma ADMA levels were negatively correlated with vegetable and fruit consumption. The purpose of this study was to examine the association between serum levels of carotenoids and serum ADMA levels in Japanese subjects. Methods: We conducted a cross-sectional study of 470 subjects (203 men and 267 women) who attended a health examination in August 2011. Serum levels of several carotenoids were separately measured by high-performance liquid chromatography. Serum ADMA levels were determined by using an enzyme-linked immunosorbent assay kit. Results: In women, the multivariate-adjusted odds ratios (ORs) of elevated serum ADMA levels were significantly decreased in the highest tertile for beta-cryptoxanthin (OR 0.47, 95% CI 0.23-0.95), alpha-carotene (OR 0.39, 95% CI 0.18-0.79), and beta-carotene (OR 0.36, 95% CI 0.17-0.73) compared to the lowest tertile. In men, significantly decreased ORs were observed in the highest tertiles of serum zeaxanthin/lutein (OR 0.23, 95% CI 0.06-0.69) and acarotene (OR 0.26, 95% CI 0.07-0.82), and in the middle and the highest tertiles of serum beta-carotene (OR 0.27, 95% CI 0.09-0.74 and OR 0.20, 95% CI 0.03-0.88, respectively) when the tertile cutoff points of women were extrapolated to men. Conclusions: Higher serum levels of carotenoids, such as alpha-carotene and beta- carotene, may help to prevent elevated serum ADMA levels in Japanese subjects.
  • Toru Nishikawa, Senju Hashimoto, Naoto Kawabe, Masao Harata, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Yuko Mizuno, Hiroaki Shimazaki, Toshiki Kan, Kazunori Nakaoka, Yuka Takagawa, Masashi Ohki, Naohiro Ichino, Keisuke Osakabe, Kentaro Yoshioka
    WORLD JOURNAL OF GASTROENTEROLOGY 20(5) 1289-1297 2014年2月  査読有り
    AIM: To investigate the factors other than fibrosis stage correlating with acoustic radiation force impulse (ARFI) elastograpy in chronic hepatitis C. METHODS: ARFI elastograpy was performed in 108 consecutive patients with chronic hepatitis C who underwent a liver biopsy. The proportion of fibrosis area in the biopsy specimens was measured by computer-assisted morphometric image analysis. RESULTS: ARFI correlated significantly with fibrosis stage (beta = 0.1865, P &lt; 0.0001) and hyaluronic acid levels (beta = 0.0008, P = 0.0039) in all patients by multiple regression analysis. Fibrosis area correlated significantly with ARFI by Spearman's rank correlation test but not by multiple regression analysis. ARFI correlated significantly with body mass index (BMI)(beta = -0.0334, P = 0.0001) in F0 or F1, with gamma-glutamyltranspeptidase levels (beta = 0.0048, P = 0.0012) in F2, and with fibrosis stage (beta = 0.2921, P = 0.0044) and hyaluronic acid levels (beta = 0.0012, P = 0.0025) in F3 or F4. The ARFI cutoff value was 1.28 m/s for F &gt;= 2, 1.44 m/s for F &gt;= 3, and 1.73 m/s for F4. CONCLUSION: ARFI correlated with fibrosis stage and hyaluronic acid but not with inflammation. ARFI was affected by BMI, gamma-glutamyltranspeptidase, and hyaluronic acid in each fibrosis stage. (C) 2014 Baishideng Publishing Group Co., Limited. All rights reserved.
  • Inoue T, Suzuki K, Hamajima T, Watarai R, Kimura A, Ichino N, Kusuhara Y, Osakabe K, Sugimoto K, Yamada H, Hamajima N
    Int J Anal Bio-Sci 2(2) 71-76 2014年  査読有り
  • Naoto Kawabe, Keisuke Osakabe, Senju Hashimoto, Michihito Murao, Yoshifumi Nitta, Takuji Nakano, Hiroaki Shimazaki, Toshiki Kan, Kazunori Nakaoka, Masashi Ohki, Takagawa Yuka, Takamitsu Kurashita, Emi Matsuo, Tomoki Takamura, Aiko Fukui, Toru Nishikawa, Naohiro Ichino, Kentaro Yoshioka
    HEPATOLOGY 60 1112A-1112A 2014年  査読有り
  • 刑部恵介, 市野直浩, 西川 徹, 杉山博子, 加藤美穂, 杉本邦彦, 原田雅生, 川部直人, 橋本千樹, 吉岡健太郎
    超音波技術 39(3) 249-255 2014年  査読有り
  • 吉岡 健太郎, 橋本 千樹, 川部 直人, 原田 雅生, 西川 徹, 市野 直浩, 刑部 恵介
    肝胆膵 67(6) 1059-1064 2013年12月  
  • Kentaro Yoshioka, Hiroaki Shimazaki, Naoto Kawabe, Masao Harata, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Yuko Arima, Toshiki Kan, Masashi Ohki, Kazunori Nakaoka, Takagawa Yuka, Toru Nishikawa, Keisuke Osakabe, Naohiro Ichino, Senju Hashimoto
    HEPATOLOGY 58 480A-480A 2013年10月  査読有り
  • Naoto Kawabe, Senju Hashimoto, Masao Harata, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Hiroaki Shimazaki, Yuko Arima, Toshiki Kan, Nakaoka Kazunori, Masashi Ohki, Takagawa Yuka, Toru Nishikawa, Keisuke Osakabe, Naohiro Ichino, Kentaro Yoshioka
    HEPATOLOGY 58 901A-901A 2013年10月  査読有り
  • Toshiki Kan, Keisuke Osakabe, Naoto Kawabe, Senju Hashimoto, Masao Harata, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Yuko Arima, Hiroaki Shimazaki, Masashi Ohki, Kazunori Nakaoka, Takagawa Yuka, Toru Nishikawa, Naohiro Ichino, Kentaro Yoshioka
    HEPATOLOGY 58 1137A-1138A 2013年10月  査読有り
  • Hiroya Yamada, Koji Suzuki, Naohiro Ichino, Yoshitaka Ando, Akira Sawada, Keisuke Osakabe, Keiko Sugimoto, Koji Ohashi, Ryouji Teradaira, Takashi Inoue, Nobuyuki Hamajima, Shuji Hashimoto
    Clinica Chimica Acta 424 99-103 2013年9月3日  査読有り
    Background: In many industrialized countries, non-alcoholic fatty liver disease (NAFLD) is recognized as an important disease that increases the risk of cardiovascular disease, type-2 diabetes, and metabolic syndrome. Most people with NAFLD are asymptomatic, and the disease is discovered incidentally during clinical laboratory tests. Present screening methods for NAFLD use ultrasound, and CT scans that are time-consuming, and a simple screening method for NAFLD is needed. In this study, we investigated whether serum miRNAs are associated with NAFLD and assessed the potential of serum miRNAs as a biomarker for NAFLD. Methods: We assessed intrahepatic fat by ultrasound scan, and the serum levels of five miRNAs (miR-21, miR-34a, miR-122, miR-145, and miR-451), which help regulate cholesterol and fatty acid homeostasis in liver tissue, by real-time PCR in a cross-sectional sample of 403 participants who attended health examinations. Results: Serum levels of miRNAs, miR-21, miR-34a, miR-122, and miR-451 were higher in participants with NAFLD. The serum level of miR-122 was correlated with the severity of liver steatosis. Conclusion: Serum levels of miRNAs, particularly miR-122, may be a useful biomarker for NAFLD. © 2013 Elsevier B.V.
  • Koji Suzuki, Hisashi Honjo, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Hiroya Yamada, Yasuhiro Kusuhara, Rika Watarai, Takeshi Hamajima, Nobuyuki Hamajima, Takashi Inoue
    Journal of Epidemiology 23(6) 451-456 2013年8月31日  査読有り
    Background: Albuminuria is a risk factor for not only nephropathy progression but also cardiovascular disease. Oxidative stress may have a role in the positive association between albuminuria and cardiovascular disease. Methods: This cross-sectional study investigated the associations of serum levels of carotenoids, which are dietary antioxidants, with albuminuria among 501 Japanese adults (198 men, mean age ± SD: 66.4 ± 10.0 years 303 women, mean age ± SD: 65.4 ± 9.8 years) who attended a health examination. Serum levels of carotenoids were determined by high-performance liquid chromatography. Logistic regression analysis was used to estimate odds ratios (ORs) with 95% CIs for albuminuria after adjustment for age, body mass index, smoking habits, drinking habits, hypertension, diabetes mellitus, and dyslipidemia. Results: Prevalence of albuminuria was 15.4% among men and 18.1% among women. Among women with albuminuria, geometric mean serum levels of canthaxanthin, lycopene, ß-carotene, total carotenes, and provitamin A were significantly lower than those of normoalbuminuric women. Adjusted ORs for albuminuria among women in the highest tertiles of serum ß-carotene (OR, 0.45 95% CI, 0.20-0.98) and provitamin A (OR, 0.45 95% CI, 0.20-0.97) were significantly lower as compared with those for women in the lowest tertile. There were no associations between serum carotenoids and albuminuria in men. Conclusions: An increased level of serum provitamin A, especially serum ß-carotene, was independently associated with lower risk of albuminuria among Japanese women. © 2013 Koji Suzuki et al.
  • 高井 洋次, 高村 マキ, 野村 昌彦, 赤堀 竜一, 伊藤 慶子, 西川 徹, 杉本 邦彦, 犬塚 斉, 刑部 恵介, 市野 直浩
    超音波検査技術抄録集 38 S186-S186 2013年  
  • 西川 徹, 杉本 邦彦, 杉山 博子, 加藤 美穂, 青山 和佳奈, 鈴木 亜委, 岸 詩織, 犬塚 斉, 高井 洋次, 赤堀 竜一, 刑部 恵介, 市野 直浩
    超音波検査技術抄録集 38 S127-S127 2013年  
  • Koji Suzuki, Junichi Ishii, Fumihiko Kitagawa, Atsuhiro Kuno, Yasuhiro Kusuhara, Junichi Ochiai, Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Hiroya Yamada, Yoshinori Ito, Nobuyuki Hamajima, Takashi Inoue
    Journal of Epidemiology 23(3) 163-168 2013年  査読有り
    Background: Several epidemiologic studies have reported an inverse association between serum levels of carotenoids and cardiovascular disease risk. However, no studies have reported an association between serum carotenoids and N-terminal pro-brain-type natriuretic peptide (NT-proBNP) in the general population. Methods: In this cross-sectional study,we investigated whether serum carotenoids were associated with serum NTproBNP in 1056 Japanese subjects (390 men, 666 women) who attended a health examination. Serum levels of carotenoids were separately determined by high-performance liquid chromatography. Serum NT-proBNP level was measured by electrochemiluminescence immunoassay. Results: Serum NT-proBNP was elevated (≥55 pg/ml) in 31.8% of men and 48.2% of women. Multivariate logistic regression analyses adjusted for confounding factors showed a significant association between the highest quartile of serum a-carotene and elevated NT-proBNP in men (odds ratio [OR]= 0.40, 95% CI = 0.19-0.82, P for trend = 0.005) and women (OR = 0.62, 95% CI = 0.39-0.99, P for trend = 0.047). In women, moreover, elevated serum NTproBNP was significantly associated with serum canthaxanthin (OR = 0.57, 95% CI = 0.36-0.90 for highest quartile, P for trend = 0.026) and ß-cryptoxanthin (OR = 0.53, 95% CI = 0.32-0.85 for highest quartile, P for trend = 0.026), after adjusting for potential confounders. Conclusions: Higher levels of serum carotenoids were associated with lower risk of elevated serum NT-proBNP levels after adjusting for possible confounders, which suggests that a diet rich in carotenoids could help prevent cardiac overload in the Japanese population. © 2013 Japan Epidemiological Association.
  • 西川 徹, 杉本 邦彦, 犬塚 斉, 高井 洋次, 刑部 恵介, 市野 直浩
    超音波検査技術抄録集 37 S142-S142 2012年  
  • 高井 洋次, 高村 マキ, 伊藤 慶子, 野村 昌彦, 赤堀 竜一, 西川 徹, 刑部 恵介, 市野 直浩, 杉本 邦彦, 犬塚 斉
    超音波検査技術抄録集 37 S228-S228 2012年  
  • Naohiro Ichino, Keisuke Osakabe, Keiko Sugimoto, Akira Yokoi, Takashi Inoue, Koji Suzuki, Yasuhiro Kusuhara, Nobuyuki Hamajima, Hiroji Takai, Tadayoshi Hata
    JOURNAL OF MEDICAL ULTRASONICS 39(1) 3-9 2012年1月  査読有り
    Assessment of plaque formation in the common carotid artery (CCA) is important for stratification of the risk of subsequent stroke. The stiffness parameter beta, which can be assessed using an ultrasonic phase-locked echo-tracking system, was developed to evaluate arterial stiffness. The purpose this study was to examine the relationship of the stiffness parameter beta to intima-media thickness (IMT) and plaque formation. The stiffness parameter beta and mean IMT were measured using an ultrasonic phase-locked echo-tracking system and an automatic IMT measurement system, respectively, and the presence of plaque was assessed by ultrasonography in 347 subjects who took part in a health check-up program for community-dwelling people in Hokkaido. Values of the stiffness parameter beta were significantly higher in subjects with plaque formation than in those without plaque (10.60 +/- A 0.45 vs. 8.88 +/- A 0.23, P &lt; 0.005) in whom IMT was not thickened; however, it was not different in subjects with increased IMT (&gt; 1.0 mm). The percentage of plaque formation was significantly correlated with the value of the stiffness parameter beta. Furthermore, the stiffness parameter beta was significantly correlated with plaque formation after adjustment for several clinical variables (odds ratio 1.113-1.178, P &lt; 0.001). The stiffness parameter beta is associated with plaque formation in the CCA, especially in subjects with a normal IMT. This could potentially be used as a predictor for plaque formation.
  • Keisuke Osakabe, Naohiro Ichino, Toru Nishikawa, Hiroko Sugiyama, Miho Kato, Shiho Kitahara, Senju Hashimoto, Naoto Kawabe, Masao Harata, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Hiroaki Shimazaki, Yuko Arima, Koji Suzuki, Kentaro Yoshioka
    JOURNAL OF GASTROENTEROLOGY 46(11) 1324-1334 2011年11月  査読有り
    Background Liver stiffness (LS) has been reported to correlate with fibrosis stage (F). The correlation between LS and fibrosis stage and the reduction of LS by antiviral therapy were examined in patients with hepatitis B infection. Methods LS was measured by FibroScan in 212 patients infected with hepatitis B virus. Liver biopsies were done in 51 patients. Changes of LS were assessed in 29 patients treated with nucleotide or nucleoside analogs and 52 patients without antiviral therapy. Results LS was significantly correlated with fibrosis stage (rho = 0.686, P &lt; 0.0001). The optimal cut-off values of LS were 7.1 kPa for F &gt;= 2, 10.7 kPa for F &gt;= 3, and 16.0 kPa for F4. LS was significantly reduced by antiviral therapy, from 12.9 (range 6.2-17.9) kPa to 6.6 (4.4-10.3) kPa measured at an interval of 512 (range 366-728) days (P &lt; 0.0001). Eleven of 19 (58%) patients with baseline fibrosis stages of F3-4 deduced from LS had 2-point or greater reductions of deduced stage at the last LS measurement. The change ratio of hyaluronic acid (P = 0.0390) was associated with a 2-point or greater reduction of deduced fibrosis stage. Without antiviral therapy, LS tended to increase, increasing from 6.1 (range 3.9-8.5) kPa to 6.3 (range 4.4-9.7) kPa at an interval of 422 (range 358-709) days (P = 0.0682). Conclusions LS was significantly correlated with fibrosis stage in patients with chronic hepatitis B. The reduction of LS by antiviral therapy was significantly correlated with the reduction of hyaluronic acid. Thus, we conclude that LS can be useful to assess the progression and regression of liver fibrosis stage noninvasively.
  • Masao Harata, Senju Hashimoto, Naoto Kawabe, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Yuko Arima, Hiroaki Shimazaki, Tetsuya Ishikawa, Akihiko Okumura, Naohiro Ichino, Keisuke Osakabe, Toru Nishikawa, Kentaro Yoshioka
    HEPATOLOGY RESEARCH 41(5) 423-429 2011年5月  査読有り
    Aim: Transient elastography is a non-invasive tool to measure liver stiffness (LS), which has been reported to correlate with stage of liver fibrosis. Extrahepatic cholestasis was reported to cause elevated LS, which is considered to be attributed to the increased hydrostatic pressure in the liver. In the present study, the correlation of LS with laboratory data was investigated in extrahepatic cholestasis. The change of LS after biliary drainage was also assessed. Methods: LS was measured in 29 patients with extrahepatic cholestasis due to carcinomas in 12 and non-neoplastic diseases of biliary tract or pancreas in 17. Results: In 15 patients, LS was 11.4 kPa or higher which suggested liver cirrhosis in chronic infection of hepatitis C virus. LS significantly correlated positively with serum bilirubin levels (r = 0.726, P &lt; 0.0001) and negatively with serum aspartate aminotransferase (AST) levels (r = -0.481, P = 0.0082) and alanine aminotransferase (ALT) levels (r = -0.631, P = 0.0002). Biliary drainage led to a reduction of bilirubin by 13.5 to 0.9 mg/dL which was significantly correlated with a reduction of LS by 14.3 to 0.5 kPa (r = 0.524, P = 0.0257). Conclusion: In extrahepatic cholestasis, the elevation of LS which is probably attributed to the increased hydrostatic pressure in the liver, correlates positively with the accumulation of bilirubin but negatively with damage of hepatocytes indicated by ALT levels. Further studies on the mechanism underlying the elevation of LS should be helpful to elucidate the pathogenesis of extrahepatic cholestasis.
  • Naohiro Ichino, Keisuke Osakabe, Toru Nishikawa, Hiroko Sugiyama, Miho Kato, Shiho Kitahara, Senju Hashimoto, Naoto Kawabe, Masao Harata, Yoshifumi Nitta, Michihito Murao, Takuji Nakano, Yuko Arima, Hiroaki Shimazaki, Koji Suzuki, Kentaro Yoshioka
    WORLD JOURNAL OF GASTROENTEROLOGY 16(38) 4809-4816 2010年10月  査読有り
    AIM: To construct and evaluate a new non-invasive fibrosis index for assessment of the stage of liver fibrosis. METHODS: A new fibrosis index (Fibro-Stiffness index) was developed in 165 of 285 patients with chronic hepatitis C, and was validated in the other 120 patients where liver biopsy was performed. Its usefulness was compared with liver stiffness (LS) measured by FibroScan, the aminotransferase-to-platelet ratio index, the Forns index and the FibroIndex. RESULTS: The Fibro-Stiffness index consists of LS, platelet count and prothrombin time. The values of the Fibro-Stiffness index differed significantly between neighboring fibrosis stages except F0-F1. The area under the receiver operating characteristics curves of the Fibro-Stiffness index for prediction of F &gt;= 2 (0.90), F &gt;= 3 (0.90) and F = 4 (0.92) in the estimation group and those for F &gt;= 3 (0.93) and F = 4 (0.97) in the validation group were the highest among the 5 methods examined. The accuracy of the Fibro-Stiffness index had highest values for F &gt;= 2, F &gt;= 3 and F = 4 in both the estimation and validation groups. The diagnostic performance for F = 4 was improved by a combination of the Fibro-Stiffness index with serum hyaluronic acid level. CONCLUSION: The Fibro-Stiffness index was constructed and validated. It showed superior diagnostic performance to other indices for F &gt;= 2, 3 and 4. (C) 2010 Baishideng. All rights reserved.

MISC

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書籍等出版物

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講演・口頭発表等

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主要な所属学協会

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共同研究・競争的資金等の研究課題

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社会貢献活動

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