病態モデル先端医学研究センター

kugita masanori

  (釘田 雅則)

Profile Information

Affiliation
Senior Assistant Professor, Education and Research Center of Animal Models for Human Diseases, Fujita Health University
Degree
博士(理学)

J-GLOBAL ID
200901075074190365
researchmap Member ID
1000360004

Papers

 26
  • Saori Fukuda, Masanori Kugita, Kanako Kumamoto, Yuki Akari, Yuki Higashimoto, Shizuko Nagao, Takayuki Murata, Tetsushi Yoshikawa, Koki Taniguchi, Satoshi Komoto
    Viruses, 16(8), Jul 25, 2024  
    The live attenuated human rotavirus vaccine strain RIX4414 (Rotarix®) is used worldwide to prevent severe rotavirus-induced diarrhea in infants. This strain was attenuated through the cell culture passaging of its predecessor, human strain 89-12, which resulted in multiple genomic mutations. However, the specific molecular reasons underlying its attenuation have remained elusive, primarily due to the absence of a suitable reverse genetics system enabling precise genetic manipulations. Therefore, we first completed the sequencing of its genome and then developed a reverse genetics system for the authentic RIX4414 virus. Our experimental results demonstrate that the rescued recombinant RIX4414 virus exhibits biological characteristics similar to those of the parental RIX4414 virus, both in vitro and in vivo. This novel reverse genetics system provides a powerful tool for investigating the molecular basis of RIX4414 attenuation and may facilitate the rational design of safer and more effective human rotavirus vaccines.
  • 白水 貴大, 吉村 文, 坂田 美和, 熊本 海生航, 釘田 雅則, 八代 百合子, 鈴木 慶幸, 大畑 敬一, 秋江 靖樹, 山口 太美雄, 高橋 和男, 長尾 静子
    日本腎臓学会誌, 66(4) 657-657, Jun, 2024  
  • Yoshiki Kawamura, Satoshi Komoto, Saori Fukuda, Masanori Kugita, Shuang Tang, Amita Patel, Julianna R Pieknik, Shizuko Nagao, Koki Taniguchi, Philip R Krause, Tetsushi Yoshikawa
    Microbiology and immunology, 68(2) 56-64, Feb, 2024  
    Vaccine development for herpes simplex virus 2 (HSV-2) has been attempted, but no vaccines are yet available. A plasmid-based reverse genetics system for Rotavirus (RV), which can cause gastroenteritis, allows the generation of recombinant RV containing foreign genes. In this study, we sought to develop simian RV (SA11) as a vector to express HSV-2 glycoprotein D (gD2) and evaluated its immunogenicity in mice. We generated the recombinant SA11-gD2 virus (rSA11-gD2) and confirmed its ability to express gD2 in vitro. The virus was orally inoculated into suckling BALB/c mice and into 8-week-old mice. Serum IgG and IgA titers against RV and gD2 were measured by ELISA. In the 8-week-old mice inoculated with rSA11-gD2, significant increases in not only antibodies against RV but also IgG against gD2 were demonstrated. In the suckling mice, antibodies against RV were induced, but gD2 antibody was not detected. Diarrhea observed after the first inoculation of rSA11-gD2 in suckling mice was similar to that induced by the parent virus. A gD2 expressing simian RV recombinant, which was orally inoculated, induced IgG against gD2. This strategy holds possibility for genital herpes vaccine development.
  • 白水 貴大, 吉村 文, 坂田 美和, 熊本 海生航, 釘田 雅則, 高橋 和男, 長尾 静子
    日本腎臓学会誌, 65(3) 317-317, May, 2023  
  • 白水 貴大, 吉村 文, 坂田 美和, 熊本 海生航, 釘田 雅則, 高橋 和男, 長尾 静子
    日本腎臓学会誌, 65(3) 317-317, May, 2023  

Misc.

 60

Books and Other Publications

 1

Presentations

 40

Teaching Experience

 4

Professional Memberships

 4

Research Projects

 8

Social Activities

 2