研究者業績

磯部 一郎

isobe ichiro

基本情報

所属
藤田医科大学 医学部 医学科 法医学 教授
学位
博士(医学)(名古屋市立大学)

J-GLOBAL ID
200901028150391342
researchmap会員ID
5000029856

MISC

 6
  • 中留真人, 平田ゆかり, 濱島 誠, 磯部一郎
    DNA多型 21 270-273 2013年  
  • 中留真人, 折井みなみ, 濱島誠, 平田ゆかり, 日比淑江, 布谷美弥, 磯部一郎
    DNA多型 20 298-302 2012年  
  • Masato Nakatome, Minami Orii, Makoto Hamajima, Yukari Hirata, Misato Uemura, Sayaka Hirayama, Ichiro Isobe
    LEGAL MEDICINE 13(4) 205-209 2011年7月  
    DNA methylation in gene promoter regions influences gene expression. Circadian clock genes play an important role in the formation of a biological clock and aberrant methylation of these genes contributes to several disorders. In this study, we examined forensic autopsy specimens to determine whether DNA methylation status in the promoter regions of nine circadian clock genes (Per1, Per2, Per3, Cry1, Cry2, Bmal1, Clock, Tim, and Ck1e) is related to a change in acquired diathesis and/or causes of death. Methylation-specific PCR and direct sequencing methods revealed that the promoters of Per1, Cry2, Bmal1, Clock, and Ck1e were unmethylated in all the forensic autopsy specimens, while the promoters of Per2, Per3, Cry1, and Tim were partially methylated. Methylation status varied between individuals and between tissues in the same patient. A detailed analysis of methylation patterns in the Cry1 promoter region revealed that the patterns also varied between individuals and the Cry1 promoter had highly methylated patterns in two cases that had been exposed to methamphetamine. These results suggest that the methylation status of clock gene promoters varies between individuals. Methamphetamine use may influence methylation in the Cry1 gene promoter region and disturb circadian rhythmicity. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
  • 中留真人, 折井みなみ, 濱島誠, 平田ゆかり, 植村美里, 平山沙也香, 磯部一郎
    DNA多型 19 240-244 2011年  
  • Masato Nakatome, Takuma Yamamoto, Ichiro Isobe, Ryoji Matoba
    Legal Medicine 11(6) 298-301 2009年11月  
    Inherited mutations in the human cardiac sodium channel (SCN5A) gene cause arrhythmogenic diseases such as tachyarrhythmia and bradyarrhythmia. Moreover, mutation subsets in the coding region impair SCN5A function, potentially leading to sudden cardiac death (SCD). In the present study, we performed diplotype analysis of the regulatory region of the SCN5A gene in Japanese people who died suddenly because of an unknown cause (sudden death group n = 70) and controls (n = 112). There were no significant differences at six polymorphic loci between the groups. However, 38 diplotypes of 6-nucleotide polymorphism variants were identified. One of these diplotypes-Dip.D (CTG-TC/CCG-TC)-occurred significantly more frequently in the sudden death group than in the controls (p &lt 0.01, OR = 5.18, 95% CI: 1.38-19.45). Dip.D has two variants (T-1062C and T-847G), and while it is unclear whether these directly affect mRNA expression, a common polymorphism in this region modulates SCN5A expression in vitro. Our results thus suggest that the transcription of the SCN5A Dip.D variant may be associated with arrhythmogenic diseases that can induce sudden death. © 2009 Elsevier Ireland Ltd. All rights reserved.
  • Masato Nakatome, Akitaka Miyaji, Kaori Mochizuki, Yubo Kishi, Ichiro Isobe, Ryoji Matoba
    Legal Medicine 11(1) S468-S470 2009年4月  
    Glutathione S-transferase (GST) plays a major role in the detoxification of many compounds by conjugation with glutathione. GSTM1 and T1, which are important members of the GST multigene family, are polymorphic in humans. Complete deletion of the gene results in the null genotype and loss of function. However, it is not clear whether deletion of this gene is associated with a vulnerability to methamphetamine (MAP) abuse. To clarify the potential role and mechanisms of genetic polymorphisms of GSTM1 and T1 in susceptibility to MAP abuse in the Japanese population, we investigated GSTM1 and T1 polymorphisms in subjects with diagnosed MAP-related disorders and in control groups. The risk of MAP abuse associated with GSTM1 null genotype was significantly higher only in females than in subjects with the GSTM1 genotype. GSTM1 and GSTT1 null genotype combined conferred increased risk for MAP abuse compared with GSTM1 and GSTT1 genotype combined. In conclusion, we found that GSTM1 gene deletion may contribute to a vulnerability to MAP abuse in female subjects. Moreover, we identified an association between GSTM1 and GSTT1 null genotype combined and risk of MAP abuse in the Japanese population. © 2009 Elsevier Ireland Ltd. All rights reserved.

書籍等出版物

 1

講演・口頭発表等

 17

共同研究・競争的資金等の研究課題

 4

その他

 1

教育内容・方法の工夫(授業評価等を含む)

 1
  • 件名
    授業毎にプリントの配布・画像資料の提示を行い、基礎事項の定着を図る。
    開始年月日
    2009
    終了年月日
    2013
    概要
    指定教科書の重要な医用を中心にまとめたプリントを作成し重要な知識の離開を図るとともに、法医学に特徴的な画像情報を精選してスライドで提示し、具体的な所見のイメージを把握させた。

作成した教科書、教材、参考書

 1
  • 件名
    NEWエッセンシャル法医学第5版
    終了年月日
    2012
    概要
    損傷 外傷性ショック・ショックの病態生理、その他の損傷.高取健彦監修,長尾正崇他編.NEWエッセンシャル法医学第5版.東京:医歯薬出版;2012;p143-51. を分担執筆。

その他教育活動上特記すべき事項

 3
  • 件名
    第23、33、42回医学教育ワークショップ
    開始年月日
    2010
    終了年月日
    2012
    概要
    「CBT試験問題作成」に参加。
  • 件名
    学生指導委員
    開始年月日
    2008
    終了年月日
    2012
    概要
    M4学年担任
  • 件名
    学生指導委員長
    開始年月日
    2013