Research of promotion and Support Headquarters

Mizutani Kenmei

  (水谷 謙明)

Profile Information

Affiliation
Fujita Memorial Nanakuri Institute, Fujita Health University
Degree
医学博士(藤田保健衛生大学)

J-GLOBAL ID
201001014402477768
researchmap Member ID
6000025960

External link

Research Interests

 1

Papers

 13
  • Kenmei Mizutani, Shigeru Sonoda, Hideaki Wakita, Hideto Okazaki, Yoshimitsu Katoh, Takeshi Chihara, Kan Shimpo
    NEUROREPORT, 27(9) 659-664, Jun, 2016  Peer-reviewed
    Although it has been suggested that the combination of exercise and bryostatin-1 administration may induce greater functional recovery than exercise alone, the detailed molecular mechanisms are not well known. Here, we examined the relationship between this combination treatment and monoamine dynamics in the cerebral cortex peri-infarction area to promote our understanding of these molecular mechanisms. Experimental cerebral cortex infarctions were produced by photothrombosis in rats. Voluntary exercise was initiated 2 days after surgery. Motor performance was then measured using the rotarod test. Monoamine concentrations in the perilesional cortex were analyzed by high-performance liquid chromatography. In behavioral evaluations, performance in the rotarod test was significantly increased by exercise. Moreover, performance in the rotarod test after the combination of exercise and bryostatin-1 administration was significantly greater than that after exercise alone. In the analysis of monoamines, serotonin (5-HT) concentrations were significantly higher in the groups treated with exercise and bryostatin-1. In addition, 5-HT turnover was significantly lower in the groups treated with exercise and bryostatin-1. Furthermore, the mean latency in the rotarod test showed a significant positive correlation with 5-HT levels. In immunohistochemical analysis, 5-HT immunoreactivity in the dorsal raphe nucleus was shown to be higher in the groups treated with exercise. In the present study, we detected changes in the levels of monoamines associated with the combined treatment of exercise and bryostatin-1 administration in the perilesional cortex. It has been suggested that this combination of therapies may affect 5-HT turnover and serve to increase local 5-HT concentrations in the perilesional area. Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved.
  • Daisuke Mori, Hidekazu Miyake, Kenmei Mizutani, Kan Shimpo, Shigeru Sonoda, Toshiharu Yamamoto, Shuu Fujiwara, Kin-ya Kubo
    ARCHIVES OF ORAL BIOLOGY, 65 95-101, May, 2016  Peer-reviewed
    Background and objective: Malocclusion induced by raising the bite causes chronic stress. Chronic stress leads to increased plasma corticosterone levels and impaired hippocampal function due to impaired neurogenesis or increased apoptosis in the hippocampus. The present study aimed to clarify the mechanisms underlying the impaired hippocampal function induced by the bite-raised condition in aged senescence-accelerated mouse prone 8 (SAMP8). Design: Nine-month-old aged SAMP8 mice were randomly divided into control and bite-raised groups. The vertical dimension of the bite was raised by applying resin to the molars. We evaluated newborn cell proliferation, survival, differentiation, and apoptosis in the hippocampal dentate gyrus (DG). Hippocampal brain-derived neurotrophic factor (BDNF) levels were also measured. Results: The bite-raised mice exhibited a significant decrease in proliferation, survival, and differentiation of newborn cells into neurons in the hippocampal DG compared with controls. The number of apoptotic cells in the hippocampal DG was increased at 7 and 14 days after the bite-raising procedure. Expression of BDNF protein and mRNA in the hippocampus was also decreased in the bite-raised mice. Conclusion: Bite-raised aged SAMP8 mice exhibited decreased neurogenesis, increased apoptosis in the hippocampal DG, and decreased hippocampal BDNF expression, in association with hippocampus-dependent learning and memory deficits. (C) 2016 Elsevier Ltd. All rights reserved.
  • Hiroko Kondo, Minori Kurahashi, Daisuke Mori, Mitsuo Iinuma, Yasuo Tamura, Kenmei Mizutani, Kan Shimpo, Shigeru Sonoda, Kagaku Azuma, Kin-ya Kubo
    ARCHIVES OF ORAL BIOLOGY, 61 1-7, Jan, 2016  Peer-reviewed
    Background and objective: Teeth are crucial, not only for mastication, but for overall nutrition and general health, including cognitive function. Aged mice with chronic stress due to tooth loss exhibit impaired hippocampus-dependent learning and memory. Exposure to an enriched environment restores the reduced hippocampal function. Here, we explored the effects of an enriched environment on learning deficits and hippocampal morphologic changes in aged senescence-accelerated mouse strain P8 (SAMP8) mice with tooth loss. Design: Eight-month-old male aged SAMP8 mice with molar intact or with molars removed were housed in either a standard environment or enriched environment for 3 weeks. The Morris water maze was performed for spatial memory test. The newborn cell proliferation, survival, and differentiation in the hippocampus were analyzed using 5-Bromodeoxyuridine (BrdU) immunohistochemical method. The hippocampal brain-derived neurotrophic factor (BDNF) levels were also measured. Results: Mice with upper molars removed (molarless) exhibited a significant decline in the proliferation and survival of newborn cells in the dentate gyrus (DG) as well as in hippocampal BDNF levels. In addition, neuronal differentiation of newly generated cells was suppressed and hippocampus-dependent spatial memory was impaired. Exposure of molarless mice to an enriched environment attenuated the reductions in the hippocampal BDNF levels and neuronal differentiation, and partially improved the proliferation and survival of newborn cells, as well as the spatial memory ability. Conclusion: These findings indicated that an enriched environment could ameliorate the hippocampus-dependent spatial memory impairment induced by molar tooth loss. (C) 2015 Elsevier Ltd. All rights reserved.
  • Kenmei Mizutani, Shigeru Sonoda, Hideaki Wakita, Kan Shimpo
    AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION, 94(3) 239-243, Mar, 2015  Peer-reviewed
    Recently, it has become widely known that neuronal reorganization in the perilesional cortex contributes to some improvement of hemiparesis after stroke. Here, the authors examined in vivo the effects of administration of bryostatin-1, an activator of protein kinase C, combined with voluntary exercise on functional recovery and on cortical phosphorylation of -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunit GluR1 after infarction. In behavioral evaluation, the mean latency until falling from a rotating rod in the group with exercise and administered agent at 8 days after infarction was significantly longer than that in the other groups. Although there were no significant changes in GluR1 phosphorylation between bryostatin-1 administration alone and the untreated groups, exercise induced an increase in phosphorylated-Ser845-GluR1. Moreover, combining exercise with administration led to increased phosphorylated-Ser831-GluR1. These results suggest that bryostatin-1 facilitated exercise-induced paralysis recovery, which is possibly mediated by synaptic plasticity related to an increase in synaptic transmission efficiency.
  • Hideto Okazaki, Hidehiko Beppu, Kenmei Mizutani, Sayaka Okamoto, Shigeru Sonoda
    JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, 23(6) 1703-1708, Jul, 2014  Peer-reviewed
    Predicting recovery from hemiparesis after stroke is important for rehabilitation. A few recent studies reported that the levels of some growth factors shortly after stroke were positively correlated with the clinical outcomes during the chronic phase. The aim of this study was to examine the relationships between the serum levels of growth factors (vascular endothelial growth factor [VEGF], insulin-like growth factor-I [IGF-I], and hepatocyte growth factor [HGF]) and improvement in hemiparesis in stroke patients who received rehabilitation in a postacute rehabilitation hospital. Subjects were 32 stroke patients (cerebral infarction: 21 and intracerebral hemorrhage [ICH]: 11). We measured serum levels of VEGF, IGF-I, and HGF and 5 items of the Stroke Impairment Assessment Set (SIAS) for hemiparesis on admission and at discharge. Age-matched healthy subjects (n 5 15) served as controls. Serum levels of VEGF and HGF in cerebral infarct patients on admission were higher than those in control subjects, and the serum levels of IGF-I in stroke patients were lower than those in controls. The level of HGF in ICH patients on admission was negatively correlated with gains in SIAS, and higher outliers in HGF concentration were correlated with lower gains in SIAS. Focusing on the extremely high levels of these factors may be a predictor of the low recovery from hemiparesis after stroke.

Misc.

 2

教育内容・方法の工夫(授業評価等を含む)

 13
  • 件名(英語)
    人体形態学 (骨格系・筋系・発生学・内分泌学) 講義
    開始年月日(英語)
    2014/04/22
    終了年月日(英語)
    2014/06/10
    概要(英語)
    医療科学部看護学科1年 人体形態学 (骨格系・筋系・発生学・内分泌学) 分担講義 6コマ
  • 件名(英語)
    人体形態学 (骨格系・筋系) 講義
    開始年月日(英語)
    2013/04/23
    終了年月日(英語)
    2013/05/14
    概要(英語)
    医療科学部看護学科1年 人体形態学 (骨格系・筋系) 分担講義 4コマ
  • 件名(英語)
    人体形態学 (骨格系・筋系) 講義
    開始年月日(英語)
    2012/04/24
    終了年月日(英語)
    2012/05/22
    概要(英語)
    医療科学部看護学科1年 人体形態学 (骨格系・筋系) 分担講義 4コマ
  • 件名(英語)
    組織学実習
    開始年月日(英語)
    2012/05/18
    終了年月日(英語)
    2012/06/22
    概要(英語)
    医療科学部臨床検査学科2年 組織学実習 22コマ 分担指導
  • 件名(英語)
    組織学実習
    開始年月日(英語)
    2011/05/20
    終了年月日(英語)
    2011/06/24
    概要(英語)
    医療科学部臨床検査学科2年 組織学実習 22コマ 分担指導
  • 件名(英語)
    組織学実習
    開始年月日(英語)
    2010/05/19
    終了年月日(英語)
    2010/06/25
    概要(英語)
    医療科学部臨床検査学科2年 組織学実習 22コマ 分担指導
  • 件名(英語)
    解剖学I (腎臓) 講義
    終了年月日(英語)
    2010/11/25
    概要(英語)
    医療科学部リハビリテーション学科 1年 解剖学I (腎臓) 分担講義 1コマ
  • 件名(英語)
    人体形態学
    開始年月日(英語)
    2015/04/21
    終了年月日(英語)
    2015/06/09
    概要(英語)
    看護学科1年 骨格系・筋系・内分泌・発生学 分担講義 6コマ
  • 件名(英語)
    人体構造機能学I
    開始年月日(英語)
    2015/04/13
    終了年月日(英語)
    2015/05/27
    概要(英語)
    放射線学科1年 骨格系・筋系・消化器系・循環器系 分担講義 8コマ
  • 件名(英語)
    人体形態学
    開始年月日(英語)
    2016/04/19
    終了年月日(英語)
    2016/06/07
    概要(英語)
    看護学科1年 骨格系・筋系・内分泌・発生学 分担講義 6コマ
  • 件名(英語)
    人体構造機能学I
    開始年月日(英語)
    2016/04/11
    終了年月日(英語)
    2016/05/30
    概要(英語)
    放射線学科1年 骨格系・筋系・消化器系・循環器系 分担講義 8コマ
  • 件名(英語)
    人体形態学
    開始年月日(英語)
    2017/04/25
    終了年月日(英語)
    2017/06/06
    概要(英語)
    看護学科1年 骨格系・筋系・内分泌・発生学 分担講義 6コマ
  • 件名(英語)
    人体構造機能学I
    開始年月日(英語)
    2017/04/17
    終了年月日(英語)
    2017/06/05
    概要(英語)
    放射線学科1年 骨格系・筋系・消化器系・循環器系 分担講義 8コマ

作成した教科書、教材、参考書

 2
  • 件名(英語)
    発生学・内分泌学用参考資料を作成
    終了年月日(英語)
    2014/04/22
    概要(英語)
    看護師国家試験過去100回分の発生学・内分泌学に関連した出題問題を網羅し、且つ教科書の要点を纏めた資料を作成。
  • 件名(英語)
    骨・筋学用参考資料を作成
    終了年月日(英語)
    2012/04/24
    概要(英語)
    看護師国家試験過去100回分の骨学・筋学に関連した出題問題を網羅し、且つ教科書の要点を纏めた資料を作成。