Naoki Takayanagi, Hidehiko Beppu, Kenmei Mizutani, Yutaka Tomita, Shizuko Nagao, Shoichi Suzuki, Abbas Orand, Hisahide Takahashi, Shigeru Sonoda
JOURNAL OF NEUROSCIENCE METHODS, 219(1) 162-168, Sep, 2013 Peer-reviewed
Background: Although different gait analysis methods such as Walking Track Analysis exist, they cannot be used to demonstrate the physical condition of mice with specific gait disorder characteristic. Therefore, we developed a new method for the gait analysis of such mice to accurately assess hind limb angle based on the pelvic axis.
New method: We established and verified a gait analysis method capable of pelvic axis-based limb angle measurement by video-recording the gait of a control mice group (C57BL/6J(B6)) and three ataxic mice (ataxic B6-wob/t, Parkinson's disease model (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treated (MPTP)), and cerebellum hypoplasia (cytosine-beta-D-arabinofuranoside treated)) from the ventral side.
Results: The assessed hind limb angles of B6-wob/t and MPTP-treated mice were significantly wider than B6 mice (p<0.01). Moreover, we could draw separating lines with slopes of minus one that could separate the data of each group in-the scatter plot of the normalized hind limb step width and angle.
Comparison with existing methods: We found no significance when we applied the already existing nose-tail method for the analysis of the hind limb angles of B6 and B6-woblt mice. In the nose-tail method, since the whole body axis of the trunk varies while the trunk of the mouse is laterally bent changing the hind limb angle, B6 and B6-wob/t mice could not be differentiated. However, the two mice groups could be differentiated by the pelvic axis-based gait analysis method.
Conclusion: The pelvic axis-based gait analysis method is promising and valid for mice with gait disorder. (C) 2013 Elsevier B.V. All rights reserved.