医療科学部

別府 英博

Hidehiro BEPPU

基本情報

所属
藤田保健衛生大学 医療科学部 臨床検査学科 准教授
学位
医学博士(藤田保健衛生大学)

通称等の別名
別府秀彦, 別府 秀彦
研究者番号
30142582
J-GLOBAL ID
200901037497916169
researchmap会員ID
1000102755

外部リンク

受賞

 1

論文

 108
  • Takaaki Kaneko, Takeshi Chihara, Kan Shimpo, Hidehiko Beppu, Takashi Higashiguchi, Shigeru Sonoda
    Asian Pacific Journal of Cancer Prevention 16(9) 3881-3885 2015年  査読有り
    Obesity markedly increases the risk of colorectal cancer. Recently, the preventive effects of edible mushrooms on triglyceride elevation and visceral fat accumulation have been reported. Here, the effects of Pleurotus eryngii (Eringi) and Hypsizygus marmoreus (Bunashimeji) on azoxymethane (AOM)-induced aberrant crypt foci (ACF precancerous lesions) in the colorectums of mice fed a high-fat diet were examined. Eringi (ER) and Bunashimeji (BU) mushroom powder samples were used. Six-week-old male C57BL/6J mice received an intraperitoneal injection of AOM (10 mg/kg) once a week for two weeks, and were sacrificed and dissected at 6 weeks after the start of the experiment. After the initiation of the experiment, they received a normal diet (ND), high-fat diet (HFD), HFD + ER (1 or 5% of diet), or HFD + BU (1 or 5% of diet). As a result, body and fat weights were significantly lower in the 5% ER and BU groups than in the HFD group. Liver triglyceride levels were also significantly lower in the 5% ER and BU groups. Total liver cholesterol levels were significantly lower in the 5% ER group. The numbers of ACF (especially large ACF) showed strong inhibitory effects in both ER and BU groups. Measurement of the cell proliferation marker Ki-67 labeling index in the colonic mucosa demonstrated more significant suppression in both ER and BU groups than in the HFD group. These results suggest that the simultaneous intake of ER and BU may inhibit colorectal tumorigenesis in HFD-fed mice.
  • Takeshi Chihara, Kan Shimpo, Hidehiko Beppu, Naoki Yamamoto, Takaaki Kaneko, Kazumasa Wakamatsu, Shigeru Sonoda
    Asian Pacific Journal of Cancer Prevention 16(9) 3887-3891 2015年  査読有り
    Aloe-emodin (1, 8-dihydroxy-3-hydroxyl-methylanthraquinone AE) and emodin (1,3,8-trihydroxy-6-methylanthraquinone EM) are anthraquinone derivatives that have been detected in some medical plants and share similar anthraquinone structures. AE and EM have been shown to exhibit anticancer activities in various cancer cell lines however, the inhibitory effects of these derivatives on the growth of cancer cells were previously reported to be different. Gastric cancer is the second most common cause of cancer cell death worldwide. In the present study, we examined the inhibitory effects of 0.05 mM AE and 0.05 mM EM on the proliferation of the MKN45 human gastric cancer cell line. The proliferation of MKN45 cells was significantly inhibited in AE- and EM-treated groups 24 h and 48 h after treatment. Furthermore, the inhibitory effects of EM were stronger than those of AE. The cell cycle of MKN45 cells were arrested in G0/G1 phase or G0/G1 and G2/M phases by AE and EM, respectively. However, an analysis of intracellular polyamine levels and DNA fragmentation revealed that the mechanisms underlying cell death following cell arrest induced by AE and EM differed.
  • TChihara, K Shimpo, H Beppu
    Pharmaceutica Analytica Acta, 6(3) 340 2015年  査読有り
  • Takeshi Chihara, Kan Shimpo, Takaaki Kaneko, Hidehiko Beppu, Takashi Higashiguchi, Shigeru Sonoda, Miyuki Tanaka, Muneo Yamada, Fumiaki Abe
    Asian Pacific Journal of Cancer Prevention 16(2) 683-687 2015年  査読有り
    Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.
  • BEPPU Hidehiko et
    Jpn J Compr Rehabil Sci 6 64-70 2015年  査読有り

MISC

 3

書籍等出版物

 32

講演・口頭発表等

 60

担当経験のある科目(授業)

 3

共同研究・競争的資金等の研究課題

 11

産業財産権

 1

社会貢献活動

 1

メディア報道

 25

教育内容・方法の工夫(授業評価等を含む)

 5
  • 件名
    臨床栄養学(医療科学部・臨床検査学科)
    開始年月日
    2009
    終了年月日
    2010
    概要
    臨床栄養学(医療科学部・臨床検査学科)臨床検査技師に不足している栄養学を教えた。分担講義     
  • 件名
    東洋医学・生薬(医学部・医学科)   
    開始年月日
    2009
    終了年月日
    2014
    概要
    東洋医学・生薬(医学部・医学科)医学教育で漢方医学の基本として生薬学を教えた。分担講義
  • 件名
    健康食品総論(医療科学部・臨床検査学科)食品学、栄養学、保健機能食品について講義をすすめた。それにより、医療の前に健康の増進を食品かからも得られ、一方術後患者や高齢者への栄養学の導入のによる健康回復の役割を教えた。また健康食品管理士の資格認定の必須科目である。
    開始年月日
    2009
    終了年月日
    2014
    概要
    健康食品総論(医療科学部・臨床検査学科)臨床検査技師に不足している食品学を含め健康食品管理士資格取得をかねた講義を行なった。
    分担講義
  • 件名
    大学院保健学(医療科学部大学院保健学機能代謝学)
    開始年月日
    2009
    終了年月日
    2014
    概要
    大学院保健学(代謝機能学)
  • 件名
    大学院医学科(博士課程リハビリテーション医学Ⅱ)
    開始年月日
    2009
    終了年月日
    2014
    概要
    大学院医学科(博士課程リハビリテーション医学Ⅱ)大学院生に代謝機能学およびリハビリテーション運動学などの講義と研究指導を行なった。

作成した教科書、教材、参考書

 1
  • 件名
    「医学領域における臨床検査学入門(第2版)」(藤田保健衛生大学『臨床検査学入門』編集委員会・編著;KTC中央出版;2009年)及び2009-2013における国家試験問題裏回答の追補版を学内出版した。
    概要
    同書の「臨床化学」の章を分担執筆した(下記参照)。

その他教育活動上特記すべき事項

 1
  • 件名
    自校学
    概要
    私立学校における愛校の精神を伝えるために創設者の生前の様子を紹介した。