Curriculum Vitaes

shinzato masanori

  (新里 昌功)

Profile Information

Affiliation
School of Health Sciences Faculty of Medical Management and Information Science, Fujita Health University
Degree
博士(医学)

Other name(s) (e.g. nickname)
新里 雅範
J-GLOBAL ID
200901003737244803
researchmap Member ID
1000102772

Papers

 4
  • shinzato masanori
    Clinical Pharmacology and Therapy, 26(2) 45-54, May, 2016  Peer-reviewedInvited
  • Ushio Iwamoto, Hideo Hori, Yoshihiro Takami, Yasuo Tokushima, Masanori Shinzato, Mikitomo Yasutake, Nobuya Kitaguchi
    JOURNAL OF ARTIFICIAL ORGANS, 18(4) 315-321, Dec, 2015  Peer-reviewed
    The efficacy of skin regeneration devices consisting of nonwoven filters and peripheral blood cells was investigated for wound healing. We previously found that human peripheral blood cells enhanced their production of growth factors, such as transforming growth factor beta 1 (TGF-beta 1) and vascular endothelial growth factor, when they were captured on nonwoven filters. Cells on biodegradable filters were expected to serve as a local supply of growth factors and cell sources when they were placed in wounded skin. Nonwoven filters made of biodegradable polylactic acid (PLA) were cut out as 13-mm disks and placed into cell-capturing devices. Mouse peripheral blood was filtered, resulting in PLA filters with mouse peripheral blood cells (m-PBCs) at capture rates of 65.8 +/- A 5.2 %. Then, the filters were attached to full-thickness surgical wounds in a diabetic db/db mouse skin for 14 days as a model of severe chronic wounds. The wound area treated with PLA nonwoven filters with m-PBCs (PLA/B+) was reduced to 8.5 +/- A 12.2 % when compared with day 0, although the non-treated control wounds showed reduction only to 60.6 +/- A 27.8 %. However, the PLA filters without m-PBCs increased the wound area to 162.9 +/- A 118.7 %. By histopathological study, the PLA/B+ groups more effectively accelerated formation of epithelium. The m-PBCs captured on the PLA filters enhanced keratinocyte growth factor (FGF-7) and TGF-beta 1 productions in vitro, which may be related to wound healing. This device is useful for regeneration of wounded skin and may be adaptable for another application.
  • Hideo Hori, Ushio Iwamoto, Gen Niimi, Masanori Shinzato, Yoshiyuki Hiki, Yasuo Tokushima, Kazunori Kawaguchi, Atsushi Ohashi, Shigeru Nakai, Mikitomo Yasutake, Nobuya Kitaguchi
    JOURNAL OF ARTIFICIAL ORGANS, 18(1) 55-63, Mar, 2015  Peer-reviewed
    Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2-22 mu m fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2-1.8 mu m captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor beta 1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.
  • Kazuyoshi Sakai, Ryuji Nomura, Yoshimi Hasegawa, Masanori Sinzato, Kazuhiro Nishii, Yoshimitsu Katoh, Kouji Yamada
    Okajimas Folia Anatomica Japonica, 92(2) 43-47, 2015  Peer-reviewed
    In order to elucidate the function of anti-aromatic acid decarboxylase (AADC)-only-positive cells in the alimentary canal, 5-hydroxy-L-tryptophan (5-HTP) or L-3,4-dihydroxyphenylalanine (L-DOPA) was intraperitoneally injected into the laboratory shrew, Suncus murinus, and immunohistochemical studies were conducted on continuous sections of the alimentary canal using specific antisera against tyrosine hydroxylase (TH), AADC, dopamine (DA), and serotonin (5-HT). AADC-only-positive cells localized to the epithelial layer of the alimentary canal from the stomach to the large intestine. These AADC-only-positive cells became DA- and AADC-positive cells after L-DOPA injection, and 5-HT- and AADC-positive cells after 5-HTP injection. These results strongly indicate that the AADC-only-positive cells in the alimentary canal of Suncus murinus are capable of synthesizing DA and 5-HT simultaneously upon administration of L-DOPA and 5-HTP.

Misc.

 70
  • Masanori Shinzato, Hidehiko Beppu, Kenmei Mizutani, Shigeru Sonoda, Toshihiko Katafuchi, Masataka Ifuku, Mami Hanada, Naoki Takayanagi, Kumiko Yamaguchi, Hiroyuki Nakagawa, Youzi Watanabe, Hisahide Takahashi
    Structure and Function, 12(1) 10-19, Aug, 2013  Peer-reviewed
  • Hidehiko Beppu, Kenmei Mizutani, Naoki Takayanagi, Masanori Shinzato, Shigeru Sonoda, Hisahide Takahashi
    Structure and Function, 11(2) 92-101, Mar, 2013  Peer-reviewed
  • Kazuyoshi Sakai, Hideki Imada, Masanori Shinzato, Kazuhiro Nishii, Ei-Ichi Miyachi, Yoshimitsu Katoh, Kouji Yamada
    Okajimas Folia Anatomica Japonica, 89(4) 105-112, Feb, 2013  Peer-reviewed
    After finding tonsil-like structures near the entrance of vagina of a laboratory shrew (Suncus murinus), which we subsequently designated as vaginal tonsils, we performed detailed immunohistochemical and developmental studies. The location of T and B cells in the vaginal tonsils differed from that in the palatine tonsils or that in the lymphoid nodes of other animals. The boundary between the germinal center region and the region encompassing follicular interfollicular tissue was not clear. B cells were widely distributed and very dense in the parenchyma, but they were scattered in the epithelial area (B cells were present in around 90% of the vaginal tonsil tissue). In contrast, T cells were scattered in the parenchyma and in the epithelial area (T cells were present in around 10% of the vaginal tonsil tissue). B cells were more prominent than T cells throughout the development of these structures and the epithelium was invaded by many immigrating cells. The size of the vaginal tonsils changed during postnatal development. Vaginal tonsils are structurally similar to other tonsils, and they may function to protect the vagina from infection.
  • Takaaki Kaneko, Kan Shimpo, Takeshi Chihara, Hidehiko Beppu, Akiko Tomatsu, Masanori Shinzato, Takamasa Yanagida, Tsutomu Ieike, Shigeru Sonoda, Akihiko Futamura, Akihiro Ito, Takashi Higashiguchi
    ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 13(5) 1983-1988, 2012  Peer-reviewed
    High temperature- and pressure-treated garlic (HTPG) has been shown to have enhanced antioxidative activity and polyphenol contents. Previously, we reported that HTPG inhibited 1,2-dimethylhydrazine-induced mucin depleted foci (premalignant lesions) and O-6-methylguanine DNA adduct formation in the rat colorectum. In the present study, we investigated the modifying effects of HTPG on N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)-induced pyloric stomach and small intestinal carcinogenesis in mice. Male C57BL/6 mice were given ENNG (100 mg/l) in drinking water for the first 4 weeks, then a basal diet or diet containing 2% or 5% HTPG for 30 weeks. The incidence and multiplicity of pyloric stomach and small intestinal (duodenal and jejunal) tumors in the 2% HTPG group (but not in the 5% HTPG group) were significantly lower than those in the control group. Cell proliferation of normal-appearing duodenal mucosa was assessed by MIB-5 immunohistochemistry and shown to be significantly lower with 2% HTPG (but again not 5% HTPG) than in controls. These results in dicate that HTPG, at 2% in the diet, inhibited ENNG-induced pyloric stomach and small intestinal (especially duodenal) tumorigenesis in mice, associated with suppression of cell proliferation.
  • Kazuyoshi Sakai, Genzoh Isomura, Masanori Shinzato, Hideki Imada, Keiki Yamada
    MICROSCOPY RESEARCH AND TECHNIQUE, 74(9) 819-824, Sep, 2011  Peer-reviewed
    We investigated the distribution of T lymphocytes, B lymphocytes, and S-100 protein-immunoreactive dendritic-like in the anal tonsil of the laboratory shrew, Suncus murinus. In adult animals, T lymphocytes were located mainly at the periphery of the anal tonsil, especially around small blood vessels. B lymphocytes were located in the central and subepithelial region of the anal tonsil, which includes primary lymphoid follicles, and in which there are small numbers of scattered T lymphocytes. B and T lymphocytes were distributed over 72.7 and 27.3% of the tonsillar area, respectively. However, their areas of distribution were not clearly distinguished. The areas containing B lymphocytes were enriched in S-100 protein antibody-immunoreactive cells, which exhibited a dendritic shape. These S-100-positive cells appeared to be identical to the follicular dendritic cells (FDC) seen in the follicles of lymphoid organs. These results suggest that the anal tonsils constitute one of the gut-associated lymphoid tissues (GALT), and that a function of the anal tonsil includes the capture of intruding antigens that would generate protective antibody responses. Microsc. Res. Tech. 74: 819-824, 2011. (C) 2010 Wiley-Liss, Inc.
  • 新保 寛, 金児孝晃, 千原 猛, 別府秀彦, 新里昌功, 若松一雅, 園田 茂
    医学と生物学, 153(6) 218-224, Jun, 2009  Peer-reviewed
  • Yoshimasa Maeno, Masanori Shinzato, Shigeo Nagashima, Susan R. Rittling, David T. Denhardt, Toshimitsu Uede, Koki Taniguchi
    VIRAL IMMUNOLOGY, 22(2) 139-144, Apr, 2009  Peer-reviewed
    We studied the role of osteopontin (OPN) in host responses against rotavirus (RV) infection. OPN knockout (OPN-KO) suckling mice were more susceptible to RV (strain EW) infection and showed prolonged diarrhea duration compared to wild-type (WT) suckling mice. OPN in the small intestine of WT mice was expressed after 48 h post-infection. On day 2 postinfection, mRNA levels of interleukin-1 beta, tumor necrosis factor-alpha, and interleukin-15 in OPN-KO mice were lower than in WT mice, although mRNA expression of Th-1- and Th-2-related cytokines in the small intestine were nearly the same between OPN-KO and WT mice. These results suggested that OPN is involved in innate responses against RV infection.
  • Gen Niimi, Nobuteru Usuda, Masanori Shinzato, Chiyuki Kaneko, Yoichi Nagamura, Jaime Pereda
    Italian Journal of Anatomy and Embryology, 113(1) 9-16, Jan, 2008  Peer-reviewed
    It is well known that avian yolk sac is involved in both primitive and definitive erythropoiesis during embryonic development. Definitive erythropoiesis is first detected at about 4-5 days incubation and its maximum activity is reached between day 10 and 15 of incubation, ending between days 18 and 20 of incubation. We confirmed the definitive erythropoietic foci in the chicken yolk sac throughout the 5th to 19th day of incubation by histochemical light and electron microscopy. The definitive erythropoietic foci were observed in the yolk sac endodermal layer from day 5 until day 19, just before hatching. Ultrastructurally, definitive erythropoietic foci were observed extravascularly in the yolk sac endodermal cell layer in direct contact with the vitellolysis zone. These findings provide a basis for clarifying definitive erythropoiesis in vertebrates.
  • 山本直樹, 新里昌功, 柳田隆正, 日比野 勤
    藤田学園医学会誌, 31(2) 159-161, Dec, 2007  Peer-reviewed
  • Gen Niimi, Kiyoshi Hasegawa, Nobuteru Usuda, Masanori Shinzato, Jaime Pereda, Yoichi Nagamura
    Italian Journal of Anatomy and Embryology, 112(3) 191-198, Jul, 2007  Peer-reviewed
    Light and electron microscopic examination of first-trimester and term human placental tissues were performed to identify erythrocytes containing hemoglobin in the villous trophoblast cell layer. Erythrocytes were not identified in chorionic villous epithelium at week 7 of gestation. These cells first appeared in the villous cytotrophoblast at week 8, and continued to be present in the villous cytotrophoblast until week 9, as shown by benzidine staining. At week 12 gestation, a cluster of erythrocytes was present in a villous syncytial sprout. At 40 and 41 weeks gestation, erythrocytes were located in the villous cytotrophoblast cell layer. Electron microscopic observations focused on the cytoplasm of villous cytotrophoblast at week 8, the syncytial sprout at week 12 and the cytotrophoblast cell layer at term, confirmed the presence of erythrocytes at an extravascular location, as observed by light microscopy.
  • Y Maeno, S Nakazawa, N Yamamoto, M Shinzato, S Nagashima, K Tanaka, J Sasaki, Rittling, SR, DT Denhardt, T Uede, K Taniguchi
    INFECTION AND IMMUNITY, 74(4) 2423-2427, Apr, 2006  
    Osteopontin (OPN) knockout mice (OPN-KO mice) died of Plasinodium chabaudi chabaudi infection, although wild-type (WT) mice had self-limiting infections. OPN was detected in the WT mice at 2 days postinfection. OPN-KO mice produced significantly smaller amounts of interleukin-12 and gamma interferon than WT mice produced. These results suggested that OPN is involved in Th1-mediated immunity against malaria infection.
  • Kan Shimpo, Hidehiko Beppu, Takeshi Chihara, Takaaki Kaneko, Masanori Shinzato, Shigeru Sonoda
    Asian Pacific Journal of Cancer Prevention, 7(4) 585-590, 2006  Peer-reviewed
    We examined the modifying effect of freeze-dried whole-leaf Aloe arborescens Miller var. natalensis Berger (Kidachi aloe in Japan designated as 'ALOE') on azoxymethane (AOM)-induced intestinal carcinogenesis in rats. Male F344 rats (4 weeks old) were fed basal diet or experimental diet containing 0.2% or 1% ALOE for 28 weeks. Starting two weeks later, the animals received subcutaneous injections of AOM once weekly for 10 weeks. The incidence of colorectal adenocarcinomas in the 0.2% (but not 1%) ALOE group showed a strong tendency for decrease (p = 0.056) from the control group. Further, the adenocarcinoma incidence in the entire intestine (small and large intestines) in the 0.2% ALOE group was significantly (p = 0.024) decreased compared to the control value. However, there were no significant differences in tumor multiplicities of colorectal or entire intestines among the 3 groups. In addition, we also studied the safety of long-term ingestion of ALOE as a health food or natural thickening stabilizer. Rats were fed the basal diet or 1% ALOE diet for 35 weeks without AOM treatment. Feeding with 1% ALOE did not affect most hematological and serum biochemical parameters in the rats. These results indicate that a low level of ALOE ingestion might have a mild suppressive effect on intestinal tumor growth without harmful side effects.
  • 別府秀彦, 新保 寛, 新里昌功, 千原 猛, 尾崎清香, 園田 茂
    機能性食品と薬理栄養, 2(2) 73-80, Nov, 2004  Peer-reviewed
  • K Sugiura, M Sugiura, R Hayakawa, Y Kato, N Sakamoto, A Osada, M Shinzato, M Shamoto
    INTERNATIONAL JOURNAL OF DERMATOLOGY, 43(6) 451-453, Jun, 2004  Peer-reviewed
    Common subungual tumors include fibrokeraroma, Koene's tumor, glomus tumor, epidermoid cyst, Bowen's disease and squamous cell carcinoma.(1) Solitary neurofibromas in this area are rare. Subungual neurofibromas normally grow without obvious symptoms. As nails conceal subungual tumors, only a proportion of these tumors are visible. We suggest that subungual neurofibromas might have been underreported. A rare case of solitary subungual neurofibroma not associated with von Recklinghausen's disease is reported. Only a few cases of solitary neurofibroma have previously been reported in the literature. Other cases of this tumor might go unnoticed due to lack of symptoms.
  • Takamasa Yanagida, Mikihiro Shamoto, Akiko Osada, Masanori Shinzato
    Journal of Clinical and Experimental Hematopathology, 44(1) 11-16, Apr, 2004  Peer-reviewed
    We previously observed Langerhans cells (LC) in the squamous metaplastic urinary bladder in rats fed a vitamin A-deficient diet. Here we report the finding of LC in the iliac lymph nodes, one of the abdominal lymph nodes draining to the urinary bladder. LC had previously only been observed in the superficial and hilar lymph nodes, but never in the abdominal lymph nodes. This is also the first observation of Birbeck granule (BG)-positive LC in the iliac lymph nodes. LC were not found in the transitional mucosa of the urinary bladder. In the present experiments, the LC were observed in both the squamous metaplastic mucosa and lamina propria of the urinary bladder, and also in the iliac lymph nodes, which drain into the urinary bladder. BG-positive LC may mature not only in the squamous epithelia, but also in squamous metaplastic mucosa. LC in the lamina propria of the urinary bladder might migrate to the iliac lymph nodes, which are regional lymph nodes, where they could function in antigen presentation. LC in the skin play a role in antigen-antibody interactions, and the squamous metaplastic mucosa of the urinary bladder may be a similar environment, in which LC in the mucosa can migrate to the regional lymph nodes.
  • Keiji Sugiura, Keiichi Hiramoto, Mikihiro Shamoto, Mariko Sugiura, Ritsuko Hayakawa, Yoshimi Kato, Masanori Shinzato, Akiko Osada, Nao Sakamoto, Hisahide Takahashi
    Journal of the European Academy of Dermatology & Venereology, 18(2) 148-152, Mar, 2004  Peer-reviewed
  • K Sugiura, M Sugiura, R Hayakawa, Y Kato, M Shamoto, Y Mizoguchi, N Sakamoto, M Shinzato, A Osada
    DERMATOLOGY, 208(2) 183-184, 2004  Peer-reviewed
  • K Shimpo, T Chihara, M Shinzato, H Beppu, T Kaneko, M Shamoto, H Kuzuya
    PHARMACEUTICAL BIOLOGY, 41(8) 631-636, Dec, 2003  Peer-reviewed
    We examined the modifying effects of freeze-dried whole-leaf Aloe arborescens Miller var. natalensis Berger (Japanese name, Kidachi aloe; designated as 'ALOE') on 1,2-dimethylhydrazine (DMH)-induced colorectal tumorigenesis in mice. Female ICR mice (7-weeks old) were given a basal diet or a diet containing 1, 0.5 or 0.1% ALOE for 32 weeks. One week later, all mice were injected i.p. with DMH (20 mg/kg, once weekly for 10 weeks) or vehicle (1 mM EDTA solution, pH 6.5). At 32 weeks, animals were killed by exsanguination, and the colorectums were processed for histological examination. The administration of ALOE (1, 0.5 or 0.1% in diet) did not induce diarrhea or reduction of body weight. In mice given DMH and 1% ALOE (Group 2), the incidence and multiplicity of colorectal adenomatous hyperplasias were significantly decreased as compared with mice given DMH alone (Group 1) (both p < 0.05), whereas the incidence and multiplicity of tumors (adenoma and adenocarcinoma) in Group 2 tended to be lower than those in Group 1. In addition, the incidence and multiplicity of the colorectal proliferative lesions (the total of adenomatous hyperplasias, adenomas and adenocarcinomas in mouse colorectum) in Group 2 were significantly decreased as compared with Group 1 (both p < 0.01). No colorectal proliferative lesions were found in animals that did not receive DMH. These results indicated that ALOE reduces the incidence and multiplicity of DMH-induced colorectal proliferative lesions, especially adenomatous hyperplasia, in mice.
  • M Takashi, M Matsuyama, K Furuhashi, Y Kodama, M Shinzato, M Shamoto, N Nakashima
    INTERNATIONAL JOURNAL OF UROLOGY, 10(11) 603-606, Nov, 2003  Peer-reviewed
    Approximately 30 cases of carcinoid tumor of the kidney have been reported in the English literature, including three cases found as components of teratomas. Renal composite tumors associated with somatostatinoma have not been described. A 53-year-old female presented with an incidentally found right renal cystic lesion. Computed tomography demonstrated a cystic lesion associated with a solid nodule in the right kidney and postcontrast dynamic MRI revealed enhancement of the solid nodule. The patient underwent radical nephrectomy for the kidney lesion and is now well without recurrence 21 months after the operation. From the histopathological findings we diagnosed the cystic lesion as a composite tumor composed of mucinous cystadenoma and carcinoid tumor. Immunohistochemistry demonstrated the majority of cells of in carcinoid portion to be positive for antisomatostatin staining. The present case is the first documented composite tumor of mucinous cystadenoma and somatostatinoma of the kidney.
  • G Niimi, N Usuda, M Shinzato, Y Nagamura
    ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 185(3) 201-205, Jun, 2003  Peer-reviewed
    The mouse visceral yolk sac (VYS) is widely known to play an important role as erythropoietic tissue during embryonic periods. Mouse VYS from embryonic days 9 to 12 was examined by light microscopy, electron microscopy and histochemical analysis with benzidine to detect the presence of hemoglobin with special reference to the development of VYS, the disappearance of the blood islands in VYS, and the appearance of a novel structure in the form of erythrocyte-like globules in VYS endodermal cells. The villous appearance of VYS became complicated by the development of VYS endodermal cells. The blood islands positive for the benzidine reaction were light microscopically detected on embryonic days 9, 10, and 11. They disappeared on embryonic day 12, however. Erythrocyte-like globules positive for the benzidine reaction were not observed in VYS endodermal cells on embryonic days 9, 10, and It, but then appeared on embryonic day 12, by light and electron microscopy. Erythrocyte-like globules in VYS endodermal cells, which appear after the disappearance of blood islands in VYS, may participate in erythropoiesis during embryonic development.
  • K Sugiura, M Shamoto, N Sakamoto, M Shinzato, A Osada, M Sugiura, R Hayakawa, Y Kato
    DERMATOLOGY, 206(3) 222-224, 2003  Peer-reviewed
    Background: Generally, Langerhans cells deliver antigen information from the skin to the draining lymph nodes via lymph vessels. Methods: By immunohistopathology, we investigated the delivery route of Langerhans cells in human skin using CD1a and S-100 protein antibodies. Results: We noted CD1a- and S-100-positive Langerhans cells in the lymph vessels of the dermis. These were shaped like dendritic cells and presented with some lymphocytes, melanophages, melanin granules and lymph in the same vessels. Conclusion: These observations support the concept that Langerhans cells deliver antigen peptides to regional lymph nodes via afferent lymph vessels. Copyright (C) 2003 S. Karger AG, Basel.
  • Kan Shimpo, Takeshi Chihara, Takaaki Kaneko, Masanori Shinzato, Hidehiko Beppu, Motoyuki Hoshino, Chikako Ida, Mikihiro Shamoto, Hiroshi Kuzuya
    Asian Pacific Journal of Cancer Prevention, 3 339-344, Dec, 2002  Peer-reviewed
  • G Niimi, N Usuda, M Shinzato, Y Nagamura
    ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 184(5) 425-429, Sep, 2002  Peer-reviewed
    Hematological studies have revealed the importance of the visceral yolk sac (VYS) in the primitive erythropoiesis of mouse embryos at an early stage before day 12. We examined the possibility of the occurrence of extra-embryonic erythropoiesis at a stage later than embryonic day 12 by light and electron microscopic analyses. Surprisingly, a novel structure in the form of erythrocyte-like globules was observed in the VYS endodermal cells. They were consistently present in the VYS endodermal cells from embryonic day 12 until day 18 (birth is day 19), by immunocytochemical and enzyme histochemical analyses. They were immuno-positive for mouse erythrocyte antibody and also positive for the benzidine reaction showing the presence of hemoglobin. The erythrocyte-like globules were shown to be the erythrocytes present in the cytoplasm. These results indicated that erythropoiesis in the VYS endodermal cells continues from the early embryonic stage, as primitive erythropoiesis, until the late stage.
  • CL Ye, Y Mizoguchi, M Tomita, M Shinzato, M Kuroda, M Shamoto, M Kasahara, M Matsuyama, A Matsuura
    NEPHROLOGY, 7(2) 61-65, Apr, 2002  Peer-reviewed
    A 73-year-old man who had been undergoing chronic haemodialysis (CHD) for 3 years developed haemophagocytic syndrome (HPS) that might have been triggered by Epstein-Barr virus (EBV) infection. The patient finally died of miliary tuberculosis (TB) reactivation that promoted the progression of HPS. Immunological abnormalities in patients undergoing CHD may be notable. The early diagnosis of TB reactivation may be important for reducing the mortality in cases of HPS, as a high incidence of TB is encountered in patients undergoing CHD. In contrast, the simultaneous occurrence of an EBER-positive hybridization signal with T cell-specific immunolabelling of CD45RO cells was well detected in the spleen and lymph nodes, and interferon gamma was elevated in the serum. These findings coincide with the reported preferential expansion of T cells rather than B cells in EBV infection, and support the hypothesis that systemic hypercytokinaemia caused by the proliferation of EBV-infected T cells may play a crucial role in the development of HPS.
  • Gen Niimi, Nobuteru Usuda, Masanori Shinzato, Yoichi Nagamura
    Journal of Creative Approach for Health, 1(1) 46-49, 2002  Peer-reviewed
  • Gen Niimi, Masanori Shinzato, Nobuteru Usuda, Yoichi Nagamura
    Journal of Analytical Bio-Science, 24(4) 324-326, 2001  Peer-reviewed
  • 千原 猛, 新保 寛, 別府秀彦, 井田千賀子, 金児孝晃, 新里雅範, 川井 薫, 廣野 巌, 社本幹博, 永津俊治, 葛谷博磁
    藤田学園医学会誌, 24(2) 223-226, Dec, 2000  Peer-reviewed
  • 新保 寛, 千原 猛, 新里雅範, 別府秀彦, 金児孝晃, 井田千賀子, 川井 薫, 廣野 巌, 社本幹博, 永津俊治, 葛谷博磁
    藤田学園医学会誌, 24(2) 217-221, Dec, 2000  Peer-reviewed
  • Kan Shimpo, Takeshi Chihara, Masanori Shinzato, Hidehiko Beppu, Takaaki Chiyuki, Kanekohikako Ida, Kaoru Kawai, Iwao Hirono, Mikihiro Shamoto, Toshiharu Nagatsu, Hiroshi Kuzuya
    Asian Pacific Journal of Cancer Prevention, 1 283-288, Dec, 2000  Peer-reviewed
  • M Shamoto, M Shinzato, B Qian, S Hosokawa, M Ishibashi
    PATHOLOGY INTERNATIONAL, 49(4) 305-309, Apr, 1999  Peer-reviewed
    The dermal histology and the regional draining superficial lymph node of a new mutant strain of hairless rats (ISh) were investigated. The homozygote ISh rat was characterized as having naked and wrinkled skin. The comedo-like cysts in the skin resembled human acne and vulgaris. Histopathologically, many Langerhans' cells positive for anti-protein kinase C type II antibody (PKC-II) were recognized in the epidermis. The superficial lymph nodes were significantly larger than those of the heterozygote. The paracortex of these lymph nodes was expanded and the number of Langerhans' cells mainly increased in these areas. These lymph node lesions were similar to those of human dermatopathic lymphadenopathy. The ISh rats should be a very useful animal model for studying dermatopathic lymphadenopathy in humans. Furthermore, they may be a valuable animal model for investigating the mechanisms of not only maturation, movement and migration of Langerhans' cells, but also of their function for antigen presentation.
  • 吉田麻子, 新里雅範, 長田明子, 細川 曉, 社本幹博
    日本リンパ網内系学会会誌, 37(1) 17-22, Aug, 1997  Peer-reviewed
  • SUZUKI Toshiaki, SHAMOTO Mikihiro, SHINZATO Masanori, MASAKI Sayaka
    The Journal of the Japanese Society of Clinical Cytology, 36(3) 319-323, May, 1997  
  • M Shamoto, A Osada, M Shinzato, C Kaneko, A Yoshida
    PATHOLOGY INTERNATIONAL, 46(5) 348-354, May, 1996  Peer-reviewed
    In the present study, immunohistochemical and immunoelectron microscopic techniques were used to differentiate Langerhans cells (LC) from interdigitating cells (IDC) in the lymph nodes (LN) of dermatopathic lymphadenopathy. The majority of the dendritic cells that existed in the LN of dermatopathic lymphadenopathy were positive for OKT-6 (CD 1a) antibody. It was concluded that these dendritic cells were not IDC, but LC. Electron microscopically, LC in these LN contained a few Birbeck granules (BG). In order to prove the fact that these dendritic cells were LC, the existence of BG was investigated ultrastructurally by examining serial sections, and immunoelectron microscopically for CD 1a positive cells. Most of the LC in the lymph nodes we examined were negative for the anti-proliferating nuclear antigen (PCNA) antibody. This finding may mean that LC in the LN are fully developed cells and do not divide in the LN. Langerhans cells may migrate from the skin lesions to the paracortical areas in the LN, which then may become enlarged.
  • Yoshida Asako, Shinzato Masanori, Hosokawa Satoru, Kozawa Hideo, Shamoto Mikihiro
    The journal of the Japanese Society of Lymphoreticular Tissue research, 36(1) 45-51, Mar, 1996  Peer-reviewed
    Langerhans cells (LCs) in the squamous metaplastic epithelium of the trachea and the urinary bladder of vitamin A-deficient rats were examined. Both round, immature cells which were considered to be precursor LCs, and mature LCs with prominent dendritic processes, were recognized in the present study. Both cells were positive for anti-protein kinase type II antibody (PKC-II), and although the former did not have Birbeck granules (BGs), the latter did. The precursor LCs without BGs first appeared in the early stage of squamous metaplasia. As the differentiation of squamous metaplasia progressed, the number of BG bearing LCs increased, and the precursor cells became mature LCs in the later stage of squamous metaplasia of both the trachea and the urinary bladder. In the later stage of squamous metaplasia of the urinary bladder, LCs with BGs also appeared in the lamina propria. These findings indicate that precursor cells which probably migrate from the bone marrow, disperse into the metaplastic squamous epithelium in morphologically immature forms, and mature into BG bearing LCs as a result of contact with squamous epithelium.
  • Chiyuki Kaneko, Mikihiro Shamoto, Hajime Niimi, Akiko Osada, Miyuki Shimizu, Masanori Shinzato
    Acta Cytologica, 40(3) 417-422, 1996  Peer-reviewed
    OBJECTIVE: To successively examine intranuclear inclusions and nuclear grooves in the same papillary thyroid cancer specimens using a light microscope (LM), scanning electron microscope (SEM) and transmission electron microscope (TEM). STUDY DESIGN: We stained cells by the Papanicolaou method after fixation in 1.25% glutaraldehyde for LM and then attempted to observe them successively by SEM-TEM after fixation in 2% paraformaldehyde and 2% osmium tetroxide. RESULTS: On SEM, intranuclear inclusions were observed as elevated parts, like hills, and nuclear grooves were observed as deep fissures or shallow cracks, sometimes with a few in one cell. On TEM, both intranuclear inclusions and nuclear grooves seemed formed by the nuclear membranes. Intranuclear inclusions also possessed cytoplasm and/or cytoplasmic organelles within some expanded areas in the nuclear grooves. CONCLUSION: In was evident from our three-step technique that intranuclear inclusions and nuclear grooves were essentially the same structures. © 1996 The International Academy of Cytology Acta Cytologica.
  • Mikihiro Shamoto, Masanori Shinzato
    Med. J. Kagoshima Univ, 47(Suppl. 2) 111-112, Nov, 1995  
  • M SHINZATO, M SHAMOTO, S HOSOKAWA, C KANEKO, A OSADA, M SHIMIZU, A YOSHIDA
    BIOTECHNIC & HISTOCHEMISTRY, 70(3) 114-118, May, 1995  Peer-reviewed
    The present study shows that Langerhans cells can be differentiated from interdigitating cells at the light microscopic level. Superficial lymph nodes and skin taken from necropsies and the lymph nodes of dermatopathic lymphadenopathy (DPL) were used for this experiment. Sections of lymph node and skin were embedded using the acetone, methyl benzoate and xylene (AMeX) method and dendritic cells were immunostained with anti S-100 protein antibody (S-100, and OKT-6 (CD1a) using the restaining method. Langerhans cells in the skin were positive for both CD1a and S-100. Dendritic cells positive for both CD1a and S-100, and dendritic cells positive for S-100, but not for CD1a were observed in superficial lymph nodes. In normal superficial lymph nodes, there were more interdigitating cells than Langerhans cells. The majority of the dendritic cells in the DPL were Langerhans cells. We conclude that the S-100 and CD1a positive cells are Langerhans cells, and the S-100 positive-CD1a negative cells are interdigitating cells.
  • 清水深雪, 長田明子, 新里雅範, 金子千之, 社本幹博, 岩瀬輝彦, 藤本正夫, 乾 和郎, 山雄健次, 中澤三郎
    藤田学園医学会誌, 18(2) 255-263, Feb, 1995  Peer-reviewed
  • M Shamoto, A Osada, M Shinzato, C Kaneko, M Shimizu
    DENDRITIC CELLS IN FUNDAMENTAL AND CLINICAL IMMUNOLOGY, VOL 2, 378 139-141, 1995  Peer-reviewed
  • C KANEKO, H NIIMI, M SHINZATO, M SHAMOTO
    DIAGNOSTIC CYTOPATHOLOGY, 11(4) 333-342, Dec, 1994  Peer-reviewed
    The identification of cells in body cavities of cancer patients is sometimes difficult to make. In order to make a definite cytological diagnosis, we observed the same cells by using light microscopy (LM)-scanning electron microscopy (SEM)-transmission electron microscopy (TEM). In this study we first stained cells by the Papanicolaou method after fixation in 1% glutaraldehyde for LM, and then attempted to observe them successively by SEM-TEM after fixation in 1% paraformaldehyde and 1.25% OsO4. Our method and procedures in examining successively one and the same cells in body cavity fluids by using LM, SEM, and TEM ensured accurate discrimination among adenocarcinoma cells, mesothelial cells, and macrophages. The results of this study suggest that LM-SEM-TEM may be of diagnostic value in distinguishing among mesothelial cells, macrophages, and adenocarcinoma cells. This method also succeeded in disclosing differences between the ultrastructure of the cell surfaces, and those of the cytoplasm, and of the nuclei. It is desirable that LM-SEM-TEM observation can be introduced into various aspects in order to obtain an improvement in the diagnosis by cytologic examination, the judgment of therapeutical effects, drug selection, and prognostic presumption. (C) 1994 Wiley-Liss, Inc.
  • OSADA Akiko, SHAMOTO Mikihiro, SHINZATO Masanori, KANEKO Chiyuki, SHIMIZU Miyuki, NIIMI Hajime
    The Journal of the Japanese Society of Clinical Cytology, 33(6) 1140-1144, Nov, 1994  Peer-reviewed
  • 社本幹博, 長田明子, 新里雅範, 細川 暁
    日本網内系学会会誌, 34(4.5) 33-38, Sep, 1994  Peer-reviewed
  • 長田明子, 新里雅範, 金子千之, 細川 暁, 清水深雪, 社本幹博
    日本網内系学会会誌, 34(3) 1-12, Jun, 1994  Peer-reviewed
  • NIIMI Hajime, KANEKO Chiyuki, SHINZATO Masanori, SHAMOTO Mikihiro
    The Journal of the Japanese Society of Clinical Cytology, 33(1) 43-52, Jan, 1994  Peer-reviewed
  • 新里雅範, 鳥山和彦, 細川 暁, 金子千之, 野本和孝, 長田明子, 清水深雪, 社本幹博
    藤田学園医学会誌, 16(2) 129-133, Mar, 1993  Peer-reviewed
  • 鳥山和彦, 新里雅範, 野本和孝, 清水深雪, 長田明子, 社本幹博
    藤田学園医学会誌, 16(2) 111-116, Mar, 1993  Peer-reviewed
  • S HOSOKAWA, M SHINZATO, C KANEKO, M SHAMOTO
    VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY, 63(3) 159-166, Mar, 1993  Peer-reviewed
    The migration and maturation of Langerhans cells (LCs) in rat tracheal squamous metaplasia due to vitamin A deficiency were investigated immunohistochemically and electron microscopically. In the early stage of metaplasia, i.e. basal cell hyperplasia, no LCs with Birbeck granules (BGs) could be found, but there were desmosome-free cells which had the morphological charcteristics of immature LCs. They were clearly different from inflammatory cells such as macrophages and lymphocytes, and were, therefore, considered to be precursors of LCs. In the stage of stratification, small numbers of Ia- and protein kinase C type II (PKCII)-positive cells were recognized. Ultrastructually they were immature LCs with ovoid nuclei, many free ribosomes and few dendrites. The cytoplasm was dark and a few BGs and atypical granules (AGs) could be seen in the Golgi area. In the early stage of cornification, LCs with partially intended nuclei, prominent nucleoli and well-developed Golgi complexes were found. There were many BGs and AGs and structures transitional between them in the Golgi areas. In epithelium showing mature squamous metaplasia, many Ia- and PKCII-positive dendritic cells could be seen. Most of these were typical mature LCs with lobulated nuclei, clear cytoplasm and prominent dendritic processes. The number of BGs and AGs was fewer than in the LCs found in the early stage of cornification, and these granules were distributed throughout the cytoplasm. In the final stage, where the basal cells had differentiated into a flatter epithelium, few LCs could be seen. These findings suggest that the precursors of LCs without BGs migrate into metaplastic squamous epithelium and mature into LCs forming BGs after exposure to the microenvironment of the squamous epithelium.
  • S HOSOKAWA, M SHINZATO, C KANEKO, M SHAMOTO
    DENDRITIC CELLS IN FUNDAMENTAL AND CLINICAL IMMUNOLOGY, 329 617-621, 1993  Peer-reviewed
  • M SHAMOTO, S HOSOKAWA, M SHINZATO, C KANEKO
    DENDRITIC CELLS IN FUNDAMENTAL AND CLINICAL IMMUNOLOGY, 329 311-314, 1993  Peer-reviewed
  • Ryo Teradaira, Masanori Shinzato, Hidehiko Beppu, Keisuke Fujita
    Phytotherapy Research, 7 S34-S36, 1993  

Books and Other Publications

 6

Presentations

 4

教育内容・方法の工夫(授業評価等を含む)

 4
  • 件名(英語)
    平成24年度版解剖学講義ノートの改訂
    終了年月日(英語)
    2012/03/31
    概要(英語)
    医療経営情報学科1年生用の講義ノートを学生意見を参考に改訂した。
  • 件名(英語)
    平成24年度版病理学講義ノートの改訂
    終了年月日(英語)
    2012/03/31
    概要(英語)
    医療経営情報学科2年生用の講義ノートを学生意見を参考に改訂した。
  • 件名(英語)
    平成24年度版カルテ特別講義ノート・練習問題集の改訂
    終了年月日(英語)
    2012/03/31
    概要(英語)
    医療経営情報学科3年生用の講義ノート・練習問題集に新規情報を追加し、改訂した。
  • 件名(英語)
    平成24年度解剖・病理学実習書の改訂
    終了年月日(英語)
    2012/06/17
    概要(英語)
    臨床工学科2年生用の実習書に臓器系統別の病理資料を追加し、改訂した。

教育方法・教育実践に関する発表、講演等

 1
  • 件名(英語)
    第2回医療科学部相互研修FD
    終了年月日(英語)
    2009/08/04
    概要(英語)
    「組織的FDの構築と実践−よりよい授業を目指して−」に参加し、標題「より良い授業を目指して―医療経営情報学科の解剖学・病理学―」を発表した。

その他教育活動上特記すべき事項

 8
  • 件名(英語)
    藤田保健衛生大学短期大学平成21年度FD研修会
    終了年月日(英語)
    2009/10/26
    概要(英語)
    「藤田保健衛生大学を知る」に参加した。
  • 件名(英語)
    第3回医療科学部相互研修FD
    終了年月日(英語)
    2010/08/04
    概要(英語)
    「戦略的FD活動を実りあるものとするためのPDCAサイクルの位置づけ」に参加した。
  • 件名(英語)
    大学院保健学研究科講演会
    終了年月日(英語)
    2010/10/20
    概要(英語)
    ハインリヒ・ハイネ大学ドイツ糖尿病センターBurkart博士の講演会にて司会した。
  • 件名(英語)
    第4回医療科学部相互研修FD
    終了年月日(英語)
    2011/08/02
    概要(英語)
    「より良い授業のために〜話し方から成績評価まで〜」、「地震対策について」に参加した。
  • 件名(英語)
    医療科学部勉強会
    終了年月日(英語)
    2011/12/26
    概要(英語)
    「分かりやすい教育を考える『コア・カリキュラム勉強会』」に参加した。
  • 件名(英語)
    第5回医療科学部相互研修FD
    終了年月日(英語)
    2012/08/07
    概要(英語)
    「身近なFD活動と大学でのティーチング・ポートフォリオの活用」にサブコーディネータとして発表した。
  • 件名(英語)
    藤田学園広報誌私立大学われを創りき第36巻1号p.26
    終了年月日(英語)
    2013/07/20
    概要(英語)
    「新入生オリエンテーションを終えて」で依頼原稿を執筆。
  • 件名(英語)
    第6回医療科学部相互研修FD
    終了年月日(英語)
    2013/08/06
    概要(英語)
    第一部「学習の質をどう評価するか ―医療人教育におけるパフォーマンフ評価を中心に―、第二部(分科会)「資格試験対策の現状と将来展望」に参加した。