医療科学部
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  2. shinzato masanori

shinzato masanori

  (新里 昌功)

Profile Information

Affiliation
School of Health Sciences Faculty of Medical Management and Information Science, Fujita Health University
Degree
博士(医学）
Other name(s) (e.g. nickname)
新里 雅範
J-GLOBAL ID
200901003737244803
researchmap Member ID
1000102772

Papers

 4
  • shinzato masanori
    Clinical Pharmacology and Therapy, 26(2) 45-54, May, 2016  Peer-reviewedInvited
  • Ushio Iwamoto, Hideo Hori, Yoshihiro Takami, Yasuo Tokushima, Masanori Shinzato, Mikitomo Yasutake, Nobuya Kitaguchi
    JOURNAL OF ARTIFICIAL ORGANS, 18(4) 315-321, Dec, 2015  Peer-reviewed
    The efficacy of skin regeneration devices consisting of nonwoven filters and peripheral blood cells was investigated for wound healing. We previously found that human peripheral blood cells enhanced their production of growth factors, such as transforming growth factor beta 1 (TGF-beta 1) and vascular endothelial growth factor, when they were captured on nonwoven filters. Cells on biodegradable filters were expected to serve as a local supply of growth factors and cell sources when they were placed in wounded skin. Nonwoven filters made of biodegradable polylactic acid (PLA) were cut out as 13-mm disks and placed into cell-capturing devices. Mouse peripheral blood was filtered, resulting in PLA filters with mouse peripheral blood cells (m-PBCs) at capture rates of 65.8 +/- A 5.2 %. Then, the filters were attached to full-thickness surgical wounds in a diabetic db/db mouse skin for 14 days as a model of severe chronic wounds. The wound area treated with PLA nonwoven filters with m-PBCs (PLA/B+) was reduced to 8.5 +/- A 12.2 % when compared with day 0, although the non-treated control wounds showed reduction only to 60.6 +/- A 27.8 %. However, the PLA filters without m-PBCs increased the wound area to 162.9 +/- A 118.7 %. By histopathological study, the PLA/B+ groups more effectively accelerated formation of epithelium. The m-PBCs captured on the PLA filters enhanced keratinocyte growth factor (FGF-7) and TGF-beta 1 productions in vitro, which may be related to wound healing. This device is useful for regeneration of wounded skin and may be adaptable for another application.
  • Hideo Hori, Ushio Iwamoto, Gen Niimi, Masanori Shinzato, Yoshiyuki Hiki, Yasuo Tokushima, Kazunori Kawaguchi, Atsushi Ohashi, Shigeru Nakai, Mikitomo Yasutake, Nobuya Kitaguchi
    JOURNAL OF ARTIFICIAL ORGANS, 18(1) 55-63, Mar, 2015  Peer-reviewed
    Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2-22 mu m fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2-1.8 mu m captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor beta 1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.
  • Kazuyoshi Sakai, Ryuji Nomura, Yoshimi Hasegawa, Masanori Sinzato, Kazuhiro Nishii, Yoshimitsu Katoh, Kouji Yamada
    Okajimas Folia Anatomica Japonica, 92(2) 43-47, 2015  Peer-reviewed
    In order to elucidate the function of anti-aromatic acid decarboxylase (AADC)-only-positive cells in the alimentary canal, 5-hydroxy-L-tryptophan (5-HTP) or L-3,4-dihydroxyphenylalanine (L-DOPA) was intraperitoneally injected into the laboratory shrew, Suncus murinus, and immunohistochemical studies were conducted on continuous sections of the alimentary canal using specific antisera against tyrosine hydroxylase (TH), AADC, dopamine (DA), and serotonin (5-HT). AADC-only-positive cells localized to the epithelial layer of the alimentary canal from the stomach to the large intestine. These AADC-only-positive cells became DA- and AADC-positive cells after L-DOPA injection, and 5-HT- and AADC-positive cells after 5-HTP injection. These results strongly indicate that the AADC-only-positive cells in the alimentary canal of Suncus murinus are capable of synthesizing DA and 5-HT simultaneously upon administration of L-DOPA and 5-HTP.

Misc.

 70
  • Masanori Shinzato, Hidehiko Beppu, Kenmei Mizutani, Shigeru Sonoda, Toshihiko Katafuchi, Masataka Ifuku, Mami Hanada, Naoki Takayanagi, Kumiko Yamaguchi, Hiroyuki Nakagawa, Youzi Watanabe, Hisahide Takahashi
    Structure and Function, 12(1) 10-19, Aug, 2013  Peer-reviewed
  • Hidehiko Beppu, Kenmei Mizutani, Naoki Takayanagi, Masanori Shinzato, Shigeru Sonoda, Hisahide Takahashi
    Structure and Function, 11(2) 92-101, Mar, 2013  Peer-reviewed
  • Kazuyoshi Sakai, Hideki Imada, Masanori Shinzato, Kazuhiro Nishii, Ei-Ichi Miyachi, Yoshimitsu Katoh, Kouji Yamada
    Okajimas Folia Anatomica Japonica, 89(4) 105-112, Feb, 2013  Peer-reviewed
    After finding tonsil-like structures near the entrance of vagina of a laboratory shrew (Suncus murinus), which we subsequently designated as vaginal tonsils, we performed detailed immunohistochemical and developmental studies. The location of T and B cells in the vaginal tonsils differed from that in the palatine tonsils or that in the lymphoid nodes of other animals. The boundary between the germinal center region and the region encompassing follicular interfollicular tissue was not clear. B cells were widely distributed and very dense in the parenchyma, but they were scattered in the epithelial area (B cells were present in around 90% of the vaginal tonsil tissue). In contrast, T cells were scattered in the parenchyma and in the epithelial area (T cells were present in around 10% of the vaginal tonsil tissue). B cells were more prominent than T cells throughout the development of these structures and the epithelium was invaded by many immigrating cells. The size of the vaginal tonsils changed during postnatal development. Vaginal tonsils are structurally similar to other tonsils, and they may function to protect the vagina from infection.
  • Takaaki Kaneko, Kan Shimpo, Takeshi Chihara, Hidehiko Beppu, Akiko Tomatsu, Masanori Shinzato, Takamasa Yanagida, Tsutomu Ieike, Shigeru Sonoda, Akihiko Futamura, Akihiro Ito, Takashi Higashiguchi
    ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 13(5) 1983-1988, 2012  Peer-reviewed
    High temperature- and pressure-treated garlic (HTPG) has been shown to have enhanced antioxidative activity and polyphenol contents. Previously, we reported that HTPG inhibited 1,2-dimethylhydrazine-induced mucin depleted foci (premalignant lesions) and O-6-methylguanine DNA adduct formation in the rat colorectum. In the present study, we investigated the modifying effects of HTPG on N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)-induced pyloric stomach and small intestinal carcinogenesis in mice. Male C57BL/6 mice were given ENNG (100 mg/l) in drinking water for the first 4 weeks, then a basal diet or diet containing 2% or 5% HTPG for 30 weeks. The incidence and multiplicity of pyloric stomach and small intestinal (duodenal and jejunal) tumors in the 2% HTPG group (but not in the 5% HTPG group) were significantly lower than those in the control group. Cell proliferation of normal-appearing duodenal mucosa was assessed by MIB-5 immunohistochemistry and shown to be significantly lower with 2% HTPG (but again not 5% HTPG) than in controls. These results in dicate that HTPG, at 2% in the diet, inhibited ENNG-induced pyloric stomach and small intestinal (especially duodenal) tumorigenesis in mice, associated with suppression of cell proliferation.
  • Kazuyoshi Sakai, Genzoh Isomura, Masanori Shinzato, Hideki Imada, Keiki Yamada
    MICROSCOPY RESEARCH AND TECHNIQUE, 74(9) 819-824, Sep, 2011  Peer-reviewed
    We investigated the distribution of T lymphocytes, B lymphocytes, and S-100 protein-immunoreactive dendritic-like in the anal tonsil of the laboratory shrew, Suncus murinus. In adult animals, T lymphocytes were located mainly at the periphery of the anal tonsil, especially around small blood vessels. B lymphocytes were located in the central and subepithelial region of the anal tonsil, which includes primary lymphoid follicles, and in which there are small numbers of scattered T lymphocytes. B and T lymphocytes were distributed over 72.7 and 27.3% of the tonsillar area, respectively. However, their areas of distribution were not clearly distinguished. The areas containing B lymphocytes were enriched in S-100 protein antibody-immunoreactive cells, which exhibited a dendritic shape. These S-100-positive cells appeared to be identical to the follicular dendritic cells (FDC) seen in the follicles of lymphoid organs. These results suggest that the anal tonsils constitute one of the gut-associated lymphoid tissues (GALT), and that a function of the anal tonsil includes the capture of intruding antigens that would generate protective antibody responses. Microsc. Res. Tech. 74: 819-824, 2011. (C) 2010 Wiley-Liss, Inc.
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Books and Other Publications

 6
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Presentations

 4

教育内容・方法の工夫（授業評価等を含む）

 4
  • 件名（英語）
    平成24年度版解剖学講義ノートの改訂
    終了年月日（英語）
    2012/03/31
    概要（英語）
    医療経営情報学科1年生用の講義ノートを学生意見を参考に改訂した。
  • 件名（英語）
    平成24年度版病理学講義ノートの改訂
    終了年月日（英語）
    2012/03/31
    概要（英語）
    医療経営情報学科2年生用の講義ノートを学生意見を参考に改訂した。
  • 件名（英語）
    平成24年度版カルテ特別講義ノート・練習問題集の改訂
    終了年月日（英語）
    2012/03/31
    概要（英語）
    医療経営情報学科3年生用の講義ノート・練習問題集に新規情報を追加し、改訂した。
  • 件名（英語）
    平成24年度解剖・病理学実習書の改訂
    終了年月日（英語）
    2012/06/17
    概要（英語）
    臨床工学科2年生用の実習書に臓器系統別の病理資料を追加し、改訂した。

教育方法・教育実践に関する発表、講演等

 1
  • 件名（英語）
    第２回医療科学部相互研修FD
    終了年月日（英語）
    2009/08/04
    概要（英語）
    「組織的FDの構築と実践−よりよい授業を目指して−」に参加し、標題「より良い授業を目指して―医療経営情報学科の解剖学・病理学―」を発表した。

その他教育活動上特記すべき事項

 8
  • 件名（英語）
    藤田保健衛生大学短期大学平成21年度FD研修会
    終了年月日（英語）
    2009/10/26
    概要（英語）
    「藤田保健衛生大学を知る」に参加した。
  • 件名（英語）
    第３回医療科学部相互研修FD
    終了年月日（英語）
    2010/08/04
    概要（英語）
    「戦略的FD活動を実りあるものとするためのPDCAサイクルの位置づけ」に参加した。
  • 件名（英語）
    大学院保健学研究科講演会
    終了年月日（英語）
    2010/10/20
    概要（英語）
    ハインリヒ・ハイネ大学ドイツ糖尿病センターBurkart博士の講演会にて司会した。
  • 件名（英語）
    第４回医療科学部相互研修FD
    終了年月日（英語）
    2011/08/02
    概要（英語）
    「より良い授業のために〜話し方から成績評価まで〜」、「地震対策について」に参加した。
  • 件名（英語）
    医療科学部勉強会
    終了年月日（英語）
    2011/12/26
    概要（英語）
    「分かりやすい教育を考える『コア・カリキュラム勉強会』」に参加した。
  • 件名（英語）
    第５回医療科学部相互研修FD
    終了年月日（英語）
    2012/08/07
    概要（英語）
    「身近なFD活動と大学でのティーチング・ポートフォリオの活用」にサブコーディネータとして発表した。
  • 件名（英語）
    藤田学園広報誌私立大学われを創りき第36巻1号p.26
    終了年月日（英語）
    2013/07/20
    概要（英語）
    「新入生オリエンテーションを終えて」で依頼原稿を執筆。
  • 件名（英語）
    第６回医療科学部相互研修FD
    終了年月日（英語）
    2013/08/06
    概要（英語）
    第一部「学習の質をどう評価するか　―医療人教育におけるパフォーマンフ評価を中心に―、第二部（分科会）「資格試験対策の現状と将来展望」に参加した。
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