研究者業績

加藤 卓

Taku Kato

基本情報

所属
藤田医科大学 臨床検査科 准教授
学位
医学博士(岐阜大学医学部医学系研究科)

J-GLOBAL ID
201801019612715115
researchmap会員ID
B000294073

研究キーワード

 3

学歴

 1

論文

 75
  • Taku Kato, Eiji Sugihara, Yuko Hata, Kyojiro Kawakami, Yasunori Fujita, Kosuke Mizutani, Tatsuya Ando, Yasuhiro Sakai, Kouhei Sakurai, Shohei Toyota, Hidetoshi Ehara, Masafumi Ito, Hiroyasu Ito
    The Prostate 2024年9月15日  
    BACKGROUND: Androgen receptor signaling inhibitors(ARSIs) have been used to treat patients with metastatic prostate cancer (PC) and castration-resistant prostate cancer (CRPC). In this study, we aimed to identify novel serum extracellular vesicle (EV)-based biomarkers to diagnose ARSI-resistance and therapeutic targets for ARSI-resistant CRPC. METHODS: Total RNA contained in serum EVs from 5 cases of CRPC before ARSI treatment and after acquiring ARSI-resistance was subjected to RNA-sequencing. The expression changes of selected RNAs contained in EVs were confirmed in 48 cases of benign prostatic hyperplasia (BPH) and 107 PC using reverse transcription-quantitative PCR (RT-qPCR) and compared with tissue RNA expression using public datasets. RESULTS: RNA-sequencing revealed that mitochondrial oxidative phosphorylation (OXPHOS)-related genes were increased in EVs after acquiring ARSI-resistance. Among them, RT-qPCR and datasets analysis demonstrated that SDHB mRNA was upregulated after acquiring ARSI-resistance in EVs and ARSI-exposed PC tissue compared to ARSI-naïve EVs and tissue, respectively. SDHB mRNA levels both in EVs and tissue were increased in localized PC compared with BPH and decreased in advanced PC. Tissue expression of SDHB mRNA was significantly correlated with those of other OXPHOS-related genes. SDHB mRNA in EVs (EV-SDHB) was elevated among 3 out of 7 ARSI-treating patients with stable PSA levels who later progressed to ARSI-resistant CRPC. CONCLUSIONS: The levels of OXPHOS-related mRNAs in EVs correlated with those in PC tissue, among which SDHB mRNA was found to be a novel biomarker to diagnose ARSI-resistance. EV-SDHB may be useful for early diagnosis of ARSI-resistance.
  • Kouhei Sakurai, Tatsuya Ando, Yasuhiro Sakai, Yuichiro Mori, Satoru Nakamura, Taku Kato, Hiroyasu Ito
    Human cell 37(5) 1559-1566 2024年9月  
    Lung neuroendocrine neoplasms (NENs) are a diverse group of tumors characterized by neuroendocrine (NE) differentiation. Among lung NENs, lung carcinoid (LC) is a rare tumor with unique characteristics. Recent research has highlighted the importance of transcription factors (TFs) in establishing gene expression programs in lung NENs such as small cell lung carcinoma. However, the TFs that control the gene expression of LC are largely unknown. In this study, we report the expression and potential function of a TF called Prospero homeobox protein1 (PROX1) in LC. Publicly available transcriptome data suggested that PROX1 was highly expressed in LC tissues, which was confirmed by immunohistochemical analysis on a tissue microarray. Knockdown of PROX1 did not impact the cellular viability of an LC-derived cell line, NCI-H727. Meanwhile, transcriptome analysis revealed that PROX1 knockdown altered the expression of genes involved in NE differentiation. ASCL1, CHGA, CALCA, and LINC00261 were suggested as downstream genes of PROX1. These findings indicate that PROX1 may play an important role in the NE identity of LC by regulating the expression of key target genes.
  • Kouhei Sakurai, Seiji Yamada, Rika Ito, Mako Ochiai, Tatsuya Ando, Yasuhiro Sakai, Taku Kato, Hiroyasu Ito
    Non-coding RNA Research 9(1) 76-83 2024年3月  査読有り
  • TAKU KATO, KYOJIRO KAWAKAMI, KOSUKE MIZUTANI, TATSUYA ANDO, YASUHIRO SAKAI, KOUHEI SAKURAI, SHOHEI TOYOTA, HIDETOSHI EHARA, HIROYASU ITO, MASAFUMI ITO
    Cancer Genomics - Proteomics 20(5) 456-468 2023年8月28日  
  • KOUHEI SAKURAI, AKIRA NAGAI, TATSUYA ANDO, YASUHIRO SAKAI, YUKA IDETA, YUICHIRO HAYASHI, JUNICHI BABA, KENJI MITSUDO, MASAHARU AKITA, NOBUTAKE YAMAMICHI, HIDETSUGU FUJIGAKI, TAKU KATO, HIROYASU ITO
    Cancer Genomics - Proteomics 20(1) 64-74 2023年1月  査読有り

MISC

 19
  • 飯沼 光司, 前川 由佳, 堀江 憲吾, 中根 慶太, 加藤 卓, 水谷 晃輔, 棚橋 裕吉, 川田 紘資, 松尾 政之, 土屋 朋大, 古家 琢也
    移植 54(総会臨時) 278-278 2019年9月  
  • 古家 琢也, 中根 慶太, 村松 由佳, 川, 飯沼 光司, 菱田 勢始, 谷口 友規, 伊藤 祐基, 加藤 大貴, 堀江 憲吾, 加藤 卓, 水谷 晃輔, 土屋 朋大
    日本ミニマム創泌尿器内視鏡外科学会雑誌 11(1) 91-94 2019年8月  
    2018年にロボット支援膀胱全摘除術が保険収載となったことにより、今後施行症例が増加すると思われる。しかし、膀胱全摘除術は、周術期合併症および死亡率とも未だ高い術式である。そのため、導入初期はプロクターによる指導や、適応の是非について慎重に検討する必要がある。一方尿変向術は、体腔内で尿路変向を行う。いわゆるICUDはあまり行われていない。ICUD普及のために、術式の標準化が必要と思われる。(著者抄録)
  • 仲野 正博, 飯沼 光司, 加藤 卓, 亀山 紘司, 山口 尊弘, 田中 秀和, 松尾 政之, 古家 琢也
    日本泌尿器科学会総会 107回 PP2-007 2019年4月  
  • 大澤 華織, 竹内 慎一, 飯沼 光司, 前川 由佳, 堀江 憲吾, 加藤 卓, 山田 佳輝, 中根 慶太, 水谷 晃輔, 土屋 朋大, 安田 満, 仲野 正博, 酒々井 夏子
    泌尿器科紀要 64(12) 524-524 2018年12月  
  • Taku Kato, Yutaka Hashimoto, Shigekatsu Maekawa, Marisa Shiina, Mitsuho Imai-Sumida, Pritha Dasgupta, Priyanka Kulkarni, Soichiro Yamamura, Shahana Majid, Sharanjot Saini, Varahram Sharryari, Guoren Deng, Rajvir Dahiya, Yuichiro Tanaka
    JOURNAL OF UROLOGY 197(4) E164-E164 2017年4月  
  • 高木 公暁, 高井 学, 河田 啓, 堀江 憲吾, 菊地 美奈, 加藤 卓, 水谷 晃輔, 清家 健作, 土屋 朋大, 安田 満, 横井 繁明, 仲野 正博, 出口 隆, 酒々井 夏子, 宮崎 龍彦, 石原 哲, 亀井 信吾
    泌尿器科紀要 62(11) 610-610 2016年11月  
  • 加藤 大貴, 加藤 卓, 後藤 高広, 玉木 正義, 米田 尚生, 田中 卓二
    日本泌尿器科学会総会 104回 PP1-204 2016年4月  
  • 仲野 正博, 亀山 紘司, 加藤 卓, 田中 秀和, 加藤 博基, 林 真也, 宇野 裕巳, 菅原 崇, 大宝 和博, 出口 隆
    日本泌尿器科学会総会 104回 PP1-228 2016年4月  
  • Takashi Deguchi, Mitsuru Yasuda, Kyoko Hatazaki, Koji Kameyama, Kengo Horie, Taku Kato, Kohsuke Mizutani, Kensaku Seike, Tomohiro Tsuchiya, Shigeaki Yokoi, Masahiro Nakano, Mutsumasa Yoh
    Emerging Infectious Diseases 22(1) 142-144 2016年1月1日  
  • Yasunori Fujita, Toshio Kojima, Kyojiro Kawakami, Kosuke Mizutani, Taku Kato, Takashi Deguchi, Masafumi Ito
    The Prostate 75(14) 1568-78 2015年10月  査読有り
    BACKGROUND: The acquisition of drug resistance is one of the most malignant phenotypes of cancer and identification of its therapeutic target is a prerequisite for the development of novel therapy. MicroRNAs (miRNAs) have been implicated in various types of cancer and proposed as potential therapeutic targets for patients. In the present study, we aimed to identify miRNA that could serve as a therapeutic target for taxane-resistant prostate cancer. METHODS: In order to identify miRNAs related to taxane-resistance, miRNA profiling was performed using prostate cancer PC-3 cells and paclitaxel-resistant PC-3 cell lines established from PC-3 cells. Microarray analysis of mRNA expression was also conducted to search for potential target genes of miRNA. Luciferase reporter assay was performed to examine miRNA binding to the 3'-UTR of target genes. The effects of ectopic expression of miRNA on cell growth, tubulin polymerization, drug sensitivity, and apoptotic signaling pathway were investigated in a paclitaxel-resistant PC-3 cell line. RESULTS: The expression of miR-130a was down-regulated in all paclitaxel-resistant cell lines compared with parental PC-3 cells. Based on mRNA microarray analysis and luciferase reporter assay, we identified SLAIN1 as a direct target gene for miR-130a. Transfection of a miR-130a precursor into a paclitaxel-resistant cell line suppressed cell growth and increased the sensitivity to paclitaxel. Lastly, ectopic expression of miR-130a did not affect the polymerized tubulin level, but activated apoptotic signaling through activation of caspase-8. CONCLUSIONS: Our results suggested that reduced expression of miR-130a may be involved in the paclitaxel-resistance and that miR-130a could be a therapeutic target for taxane-resistant prostate cancer patients.
  • 仲野 正博, 田中 秀和, 加藤 卓, 菅原 崇, 清家 健作, 亀山 紘司, 近藤 啓美, 高井 学, 秋田 和利, 大宝 和博, 田中 修, 林 真也, 宇野 裕巳, 横井 繁明, 出口 隆
    日本癌治療学会誌 50(3) 745-745 2015年9月  
  • Kimiaki Takagi, Manabu Takai, Koji Kameyama, Kengo Horie, Mina Kikuchi, Taku Kato, Kosuke Mizutani, Kensaku Seike, Tomohiro Tsuchiya, Mitsuru Yasuda, Shigeaki Yokoi, Natsuko Suzui, Masahiro Nakano, Takashi Deguchi
    Urology case reports 3(5) 138-40 2015年9月  査読有り
    A 26-year-old woman with gross hematuria was seen in a previous hospital. Magnetic resonance imaging (MRI) showed a tumor at the dome of the urinary bladder with invasion outside of the bladder wall. The patient underwent transurethral resection of the bladder tumor (TUR-BT). From the result of the pathological examination, the tumor was suggested to be carcinosarcoma of the bladder. The patient was then referred to our hospital for treatment. We performed radical cystectomy and ileal conduit diversion. Pathological examination of the excised specimen revealed an inflammatory myofibroblastic tumor as the basis for immunostaining of anaplastic lymphoma kinase (ALK).
  • 河田 啓, 高井 学, 亀山 紘司, 堀江 憲吾, 菊地 美奈, 加藤 卓, 水谷 晃輔, 清家 健作, 土屋 朋大, 横井 繁明, 仲野 正博, 出口 隆, 片桐 恭雄, 酒々井 夏子, 宮崎 龍彦
    泌尿器科紀要 61(6) 254-254 2015年6月  
  • Mina Kikuchi, Koji Kameyama, Kengo Horie, Kosuke Mizutani, Kensaku Seike, Taku Kato, Takashi Sugawara, Tomohiro Tsuchiya, Mitsuru Yasuda, Shigeaki Yokoi, Masahiro Nakano, Takashi Deguchi, Hiroshi Kondo, Yoji Moriyama, Hidetoshi Ehara
    Hinyokika kiyo. Acta urologica Japonica 60(12) 615-20 2014年12月  査読有り
    The management of urinoma after blunt renal trauma is still controversial, ranging from percutaneous drainage or ureteral stent placement for the symptomatic urinoma and waiting for spontaneous vanishment of the asymptomatic urinoma. We present two cases of symptomatic urinoma and a case of asymptomatic urinoma after renal laceration. All patients underwent selective renal arterial embolization for vascular complications, including active bleeding, pseudoaneurysm and arteriovenous fistula. Urinomas, which had been observed in all cases gradually reduced and vanished 1-24 months later. All cases were successfully managed without catheterization or percutaneous drainage for urinoma.
  • Kosuke Mizutani, Riyako Terazawa, Koji Kameyama, Taku Kato, Kengo Horie, Tomohiro Tsuchiya, Kensaku Seike, Hidetoshi Ehara, Yasunori Fujita, Kyojiro Kawakami, Masafumi Ito, Takashi Deguchi
    Anticancer research 34(7) 3419-23 2014年7月  査読有り
    BACKGROUND/AIM: Exosomes have been demonstrated to be useful non-invasive biomarkers for several cancers including prostate cancer. Since normal cells also secrete exosomes, isolation of cancer-derived exosomes from blood is a prerequisite for their better understanding. The aim of this study is to establish the method for isolation of prostate cancer-related exosomes from blood. MATERIALS AND METHODS: Exosomes were collected from prostate cancer LNCaP and PC-3 cell lines by ultracentrifugation and by using magnetic beads conjugated with anti-CD9 antibody and anti-prostate-specific membrane antigen (PSMA) antibody. Prostate cancer-related exosomes were also isolated from the plasma of prostate cancer patients by anti-PSMA beads. Isolated exosomes were analyzed by western blotting. RESULTS: Exosomes were isolated from LNCaP cells by ultracentrifugation, contained PSMA and androgen receptor (AR). AR was also detected in exosomes isolated from LNCaP cells by anti-PSMA and anti-CD9 beads, showing that AR is present in prostate cancer-related exosomes. The amount of CD9 in isolated exosomes was much higher in advanced and chemo-resistant prostate cancer patients than in prostate cancer patients without metastasis and healthy volunteers, indicating that patients with aggressive prostate cancer exhibit higher levels of prostate cancer-related exosomes in blood. CONCLUSION: The immunoaffinity-based method we developed is capable of isolating prostate cancer-related exosomes from blood, the use of which will enhance investigation processes on exosomes in prostate cancer.
  • Taku Kato, Hidetoshi Ehara, Kimiaki Takagi, Kengo Horie, Shinichi Hattori, Keita Nakane, Kensaku Seike, Takashi Sugawara, Takahiro Goto, Naruyasu Masue, Masayoshi Tamaki, Yasuhisa Ito, Takashi Deguchi
    Hinyokika kiyo. Acta urologica Japonica 57(7) 363-6 2011年7月  査読有り
    We retrospectively reviewed the records of 35 patients with penile cancer, who had been treated at Gifu University Hospital and its affiliated hospitals between July 1994 and January 2009. The mean values of follow-up periods, ages, serum squamous cell carcinoma levels and maximum diameters of the tumor were 23.7±28.0 months, 72.3±10.5 year-old, 4.5±4.3 ng/ml, and 4.0±2.6 cm, respectively. Systemic chemotherapy and local radiotherapy were performed in six, and three cases, respectively. Ten patients died of penile cancer. By univariate analyses, maximum tumor diameter (<- 4.3 cmvs >4.3 cm), T factor (<T3 vs >- T3) and N factor (<N2 vs >- N2) were significantly associated with cancer-specific survival. The five-year survival of stage N2 cases (28.6%) were significantly lower than that of stage N0 and N1 cases (68.4%) (p=0.0003). By multivariate analyses N factor (<N2 vs >- N2) was significantly associated with cancer specific survival (p=0.020). We concluded that the development of effective systemic chemotherapy might be crucial to improve the prognosis of patients with metastatic diseases.
  • 江原 英俊, 加藤 成一, 中根 慶太, 加藤 卓, 高田 俊彦, 小島 圭太郎, 亀井 信吾, 萩原 徳康, 柚原 一哉, 高橋 義人, 藤本 佳則, 藤広 茂, 蟹本 雄右, 出口 隆
    泌尿器科紀要 55(4) 199-203 2009年4月  
    We prospectively studied the usefulness of chlormadinone acetate (CMA) as an alternative therapy for prostate cancer relapse after combined androgen blockade (CAB) therapy. Sixteen patients with relapsed prostate cancer after treatment with CAB, including surgical or medical castration and nonsteroidal antiandrogens, 80 mg bicalutamide daily or 375 mg flutamide daily, were enrolled. After discontinuing the antiandrogen for evaluating the patient for the antiandrogen withdrawal syndrome, we administered 100 mg CMA daily as alternative antiandrogen andestimatedits effect. Four patients showeda >− 50% decline in prostate-specific antigen (PSA) levels andanother 4 patients showeda <50% decline in PSA levels but residual 8 patients showed no decline in PSA levels. In 8 patients with a decline in PSA levels, the median duration of alternative CMA therapy was 11.4 months. Patients with a PSA level of <1 ng/ml at the start of CMA therapy showed the tendency of decline in PSA levels. In contrast, patients with a nadir PSA level of >− 0.2 ng/ml during pretreatment showed no effectiveness of the alternative CMA therapy. The alternative CMA therapy may be useful in a part of patients with prostate cancer relapse after CAB therapy.
  • Taku Kato, Yoshito Takahashi, Keita Nakane, Shigeaki Yokoi, Hidetoshi Ehara, Ikuo Shinoda, Takashi Deguchi
    Hinyokika kiyo. Acta urologica Japonica 53(2) 117-9 2007年2月  査読有り
    A 56-year-old Japanese man consulted a urologist because of urethral bleeding. He had been undergoing hemodialysis for the past 15 years due to polycystic kidney disease. Computed tomography revealed an irregular cyst wall in the left kidney. Since a neoplasm could not be ruled out, we removed the left kidney, by laparoscopic radical nephrectomy after obtaining the patient's consent. Histopathologic diagnosis was renal cell carcinoma. Fourteen months after the operation, urethral bleeding recurred. Further examination of the bladder and the urethra revealed no significant abnormalities. The patient insisted on right nephrectomy. Therefore, laparoscopic radical nephrectomy was performed. Histopathologic diagnosis was also renal cell carcinoma. Renal cell carcinoma in patients with end-stage renal disease is fairly common and is associated with acquired cystic kidney disease. However, renal cell carcinoma associated with polycystic kidney disease is extremely rare.
  • 中根 慶太, 加藤 卓, 横井 繁明, 江原 英俊, 高橋 義人, 石原 哲, 出口 隆
    泌尿器科紀要 51(9) 631-633 2005年9月  
    75歳男.患者は膀胱前壁を中心とした多発性乳頭状広基性腫瘍で,その内の1つは内尿道口に存在していた.膀胱癌T2aN0M0と診断し,M-VAC療法1コースが施行されたが,術後14ヵ月目に外尿道口からの血性分泌物を自覚した.放置していたものの,軟性膀胱鏡検査で尿道舟状窩に充満するように存在する多発性乳頭状腫瘍を認め,膀胱癌の尿道舟状窩への再発と診断された.陰茎部分切除術を施行後,合併症なく退院なったが,腹部CTにて膵腫瘍を指摘され,化学療法が施行された.膵尾部切除により病理組織学的所見ではtubular adenocarcinomaであり,膀胱癌の膵転移は否定された.以後,患者は膵癌の腹膜播種にて最終的に死亡となったWe report a case of bladder cancer recurrence in fossa navicularis of urethra 17 months after cystourethrectomy for bladder cancer. A 75-year-old man had undergone cystourethrectomy preserving between fossa navicularis and external meatus, and ileal conduit urinary diversion for advanced bladder cancer on June 24, 2002. Histopathological findings showed urothelial carcinoma, G2>G3, pT1N0. The patient had been followed regularly for 17 months without evidence of recurrence until he suffered the onset of hemorrhagic urethral discharge. Endoscopic examination of the residual urethra showed multiple, papillary sessile tumors which almost filled the fossa navicularis. He was admitted to our hospital on December 15, 2003. The urethral wash cytology revealed urothelial carcinoma. Since computed tomography, magnetic resonance imaging, and bone scintigraphy showed no evidence of lymph node and distant metastasis, partial penectomy was performed. Histopathological findings showed urothelial carcinoma pTa, G2>G3, which was identical to primary tumor. Tumor had not invaded the corpus cavernosum. Careful follow-up of the patients with preservation of fossa navicularis is important.

共同研究・競争的資金等の研究課題

 7

社会貢献活動

 4