Profile Information
- Affiliation
- Associate Professor, Nippon Veterinary and Life Science University
- Degree
- 博士(農学)(広島大学)
- Other name(s) (e.g. nickname)
- 雜賀 愛
- J-GLOBAL ID
- 201101091439860911
- researchmap Member ID
- 6000027422
Research Interests
15Awards
1-
Jun, 2017
Papers
31-
Biochemical and Biophysical Research Communications, 729 150361-150361, Oct, 2024
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33(2) 95-104, Dec, 2022 Peer-reviewed
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Bioscience, biotechnology, and biochemistry, 86(9) 1276-1285, Aug 24, 2022 Peer-reviewedCorresponding authorImidazole dipeptides (ID) are abundant in skeletal muscle and the brain and have various functions, such as antioxidant, pH-buffering, metal-ion chelation. However, the physiological significance of ID has not been fully elucidated. In this study, we orally administered ID to conventional carnosine synthase gene-deficient mice (Carns-KO mice) to investigate the pharmacokinetics. Carnosine or anserine was administered at a dose of 500 mg (∼2 mmol) per kilogram of mouse body weight, and ID contents in the tissues were measured. No ID were detected in untreated Carns-KO mice. In the ID treatment groups, the ID concentrations in the tissues increased in a time-dependent manner in the gastrocnemius muscle, soleus muscle, and cerebrum after ID administration. Our findings suggest that the Carns-KO mice are a valuable animal model for directly evaluating the effects of dietary ID and for elucidating the physiological functions of oral ID administration.
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Biochemical and biophysical research communications, 612 22-29, Jul 5, 2022 Peer-reviewedCarnosine and anserine are abundant peptides found in the skeletal muscle and nervous system in many vertebrates. Several in vitro and in vivo studies have demonstrate that exogenously administered carnosine improves exercise performance. Furthermore, carnosine is an antioxidant and antifatigue supplement. However, the physiological functions of endogenous carnosine and its related histidine-containing dipeptides in a living organism remain unclear. We aimed to clarify the physiological roles of endogenous carnosine by investigating the characteristics of carnosine synthase gene-deficient mice and the effects of carnosine on skeletal muscle protein metabolism. We discovered that carnosine and anserine were undetectable in the skeletal muscle of carnosine synthase knockout mice. We also quantified protein gene expression and enzyme levels in muscle protein metabolism. Gene and protein levels of the muscle protein synthesizer insulin-like growth factor-1 (IGF-1) and the degrading enzyme cathepsin B were markedly lower in carnosine synthase gene-deficient mice than those in wild-type mice. The amount of 3-methylhistidine (a marker for muscle proteolysis) in forced exercise and the weight of the gastrocnemius muscle were considerably lower in carnosine synthase gene-deficient mice than in wild-type mice. Consequently, we showed that carnosine deficiency affects weight maintenance and protein metabolism in skeletal muscle, suggesting that carnosine regulates skeletal muscle protein metabolism.
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The Journal of Poultry Science, 58(3) 186-191, 2021 Peer-reviewed
Misc.
16-
Agricultural Biotechnology, 4(6) 18-22, Jun, 2020 InvitedLead authorCorresponding author
Books and Other Publications
6Presentations
61-
International Congress of Meat Science and Technology 2023, Aug 21, 2023
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International Congress of Meat Science and Technology 2022, Aug 22, 2022
Teaching Experience
5-
Livestock Processing (Toyo College of Food Technology)
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Nutrition for children and the elderly (Japan Lutheran College)
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Nutritional Chemistry (Nippon Veterinary and Life Science University)
Professional Memberships
7Research Projects
6-
科学研究費助成事業, 日本学術振興会, Apr, 2024 - Mar, 2027
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Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2024
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科学研究費補助金(基盤研究(C)), 文部科学省, Apr, 2018 - Mar, 2020
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Grants-in-Aid for Scientific Research(若手研究(B)), Ministry of Education, Culture, Sports, Science and Technology, 2012 - 2013
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Grants-in-Aid for Scientific Research(若手研究(B)), Ministry of Education, Culture, Sports, Science and Technology, 2010 - 2011