Faculty of Applied Life Science 

古田 洋樹

Furuta Hiroki

基本情報

所属
日本獣医生命科学大学 応用生命科学部 動物科学科 教授
学位
博士(農学)(九州大学)

J-GLOBAL ID
201601015390980028
researchmap会員ID
B000259469

委員歴

 6

論文

 36
  • Tatsuyuki Yoshida, Yuichi Ito, Hiroki Furuta
    The Journal of Poultry Science. 55 195-198 2018年  査読有り
  • Hiroki Furuta, Tatsuyuki Yoshida
    Journal of Animal and Veterinary Advances 16 75-77 2017年  査読有り
  • Tatsuyuki Yoshida, Yoshiyuki Ohta, Hiromi Amao, Hiroki Furuta
    Journal of Pet Animal Nutrition. 20(2) 141-144 2017年  査読有り
  • 佐伯香織, 柴野梓, 宮西玲子, 井上一歩, 津田薫, 山口ゆうき, 笹本唯衣, 吉田達行, 左向敏紀, 古田洋樹
    ペット栄養学会誌 17 1-5 2014年  査読有り
  • Hiroki Furuta, Mimaki Tanaka, Yuuna Fujiia, Tatsuyuki Yoshida
    Journal of Animal and Veterinary Advances 12(14) 1198-1201 2013年  査読有り
    It has been investigated that transgenic chicken can be produced by various methods by introducing an exogenous gene into chicken Primordial Germ Cells (PGCs). Blood containing PGCs was collected from the blood vessels of embryos at stages 12-15. An exogenous gene encoding Green Fluorescent Protein (GFP) was introduced into the PGCs by lipofection and electroporation. Electroporation was carried out under 200 V/25 μF, 200 V/200 μF and 200 V/950 μF. GFP expression was observed in PGCs. Efficiency of introduction into PGCs was low. Electroporation was superior to lipofection for the introduction of the exogenous gene into chicken PGCs. PGCs treated for GFP introduction by electroporation were injected into blood vessels of recipient embryos at stages 12-15. After incubation until stage 26, GFP bands were obtained from gonads of embryos, thereby demonstrating that treated PGCs had migrated to the gonad. © Medwell Journals, 2013.

MISC

 30

共同研究・競争的資金等の研究課題

 4