古田 洋樹

It has been investigated that transgenic chicken can be produced by various methods by introducing an exogenous gene into chicken Primordial Germ Cells (PGCs). Blood containing PGCs was collected from the blood vessels of embryos at stages 12-15. An exogenous gene encoding Green Fluorescent Protein (GFP) was introduced into the PGCs by lipofection and electroporation. Electroporation was carried out under 200 V/25 μF, 200 V/200 μF and 200 V/950 μF. GFP expression was observed in PGCs. Efficiency of introduction into PGCs was low. Electroporation was superior to lipofection for the introduction of the exogenous gene into chicken PGCs. PGCs treated for GFP introduction by electroporation were injected into blood vessels of recipient embryos at stages 12-15. After incubation until stage 26, GFP bands were obtained from gonads of embryos, thereby demonstrating that treated PGCs had migrated to the gonad. © Medwell Journals, 2013.