研究者業績

太田 能之

オオタヨシユキ  (Yoshiyuki Ohta)

基本情報

所属
日本獣医生命科学大学 動物生産化学教室 教授
学位
博士(農学)(新潟大学)

J-GLOBAL ID
201601019663256960
researchmap会員ID
B000259449

研究キーワード

 2

委員歴

 4

論文

 22
  • Jun-ichi Shiraishi, Naoko Shimakura, Kazuki Kimura, Ai-Saiga Egusa, Yoshiyuki Ohta
    The Journal of Poultry Science 61 n/a-n/a 2024年  
  • Mayako Fujihara, Jun-ichi Shiraishi, Manabu Onuma, Yoshiyuki Ohta, Miho Inoue-Murayama
    Animals 12(11) 1434-1434 2022年6月2日  
    For the conservation of endangered avian species, developing gamete preservation technologies is essential. However, studies in oocytes have not been widely conducted. In this study, assuming that the ovaries are transported to a research facility after death, we investigated the effect of ovary storage on oocytes for the purpose of cryopreserving avian female gametes by using a chicken as a model of endangered avian species. After excision, the ovaries were stored at either a low temperature (4 °C) or room temperature for 1–3 days. Ovarian follicles stored under different conditions for each period were examined by neutral red staining, histology, and gene and protein expression analysis. In addition, the pH of the storage medium after preserving the ovaries was measured. Then, ovarian tissues were vitrified to determine the cryopreservation competence. Storing the ovarian tissues at 4 °C kept the follicles viable and morphologically normal for 3 days with slow decline. In contrast, although different storage temperature did not influence follicle viability and morphology after only 1 day of storage, ovarian tissues stored at room temperature rapidly declined in structurally normal follicles, and viable follicles were rarely seen after 3 days of storage. Gene and protein expression analysis showed that apoptosis had already started on the first day, as shown by the higher expression of CASP9 under room temperature conditions. Furthermore, high expression of SOD1 and a rapid decline of pH in the storage medium under room temperature storage suggested the influence of oxidative stress associated with low pH in this condition on the follicle survivability in hen ovarian tissues. Our cryopreservation study also showed that ovarian tissues stored at 4 °C could recover after cryopreservation even after 3 days of storage. The described storage conditions and cryopreservation methods, which preserve chicken follicle survival, will lay the foundation of ovarian tissue preservation to preserve the fertility of wild female birds.
  • Shozo Tomonaga, Takahiro Kawase, Takamitsu Tsukahara, Yoshiyuki Ohta, Jun-ichi Shiraishi
    Metabolites 12(1) 86-86 2022年1月17日  
    To explore metabolic characteristics during the post-hatch developmental period, metabolomic analyses of breast muscle and plasma were performed in chickens. The most significant growth-related changes in metabolite levels were observed between seven and 28 days of age. Some of these metabolites are essential nutrients or reported as growth-promoting metabolites. In the muscle, two imidazole dipeptides—carnosine and its methylated metabolite, anserine—increased with the development. These dipeptide levels may be, in part, regulated transcriptionally because in the muscle mRNA levels of carnosine synthase and carnosine methylation enzyme increased. In contrast, taurine levels in the muscle decreased. This would be substrate availability-dependent because some upstream metabolites decreased in the muscle or plasma. In branched-chain amino acid metabolism, valine, leucine, and isoleucine decreased in the muscle, while some of their downstream metabolites decreased in the plasma. The polyamines, putrescine and spermidine, decreased in the muscle. Furthermore, mRNA levels associated with insulin/insulin-like growth factor 1 signaling, which play important roles in muscle growth, increased in the muscle. These results indicate that some metabolic pathways would be important to clarify metabolic characteristics and/or growth of breast muscle during the post-hatch developmental period in chickens.
  • 太田能之, 宮野典夫, 野口敦子, 田村直也, 宮澤美智子, 山上達彦, 宮本公寿, 高橋幸弘, 吉村映理, 宇野なつみ, 堀口政治, 長谷川悦子
    市立大町山岳博物館研究紀要 (5) 25-30 2020年3月  査読有り招待有り筆頭著者責任著者
  • Jun-Ichi Shiraishi, Hikari Shimada, Ryuzo Matsuda, Yoshiyuki Ohta
    Journal of Poultry Science 57(3) 229-235 2020年  
    Feeding behavior and energy metabolism are precisely regulated by humoral and/or neural factors in the central nervous system. In particular, nuclei, such as the arcuate nucleus, ventromedial hypothalamic nucleus, and lateral hypothalamic area located near the third ventricle of the hypothalamus are the centers of feeding and energy metabolism in various vertebrate species, including chickens. In this study, we evaluated the effects of cannulation of the third ventricle on chick growth and feeding behavior in the neonatal stage, to develop a method for local and chronic central nervous system-mediated energy metabolism. Referring to the chick brain atlas, a guide cannula was inserted into the third ventricle of the chick under anesthesia immediately after hatching using a stereotaxic instrument. The chicks that recovered from anesthesia were bred for 11 days under normal feeding management conditions, and then feed intake amount, body weight gain, and metabolic tissue weight were measured. The effects of direct stimulation of the third ventricle with 2-deoxy-D-glucose on the expression level of the immediate-early gene, cFOS, and feed intake in 5-day-old chicks were also evaluated. There were no differences in feed intake, body weight gain, and metabolic tissue weight between 11-day-old cannulated and control chicks. The expression of cFOS mRNA in the ventromedial hypothalamic nucleus was higher than that in the amygdala after the third ventricular administration of 2-deoxy-D-glucose. Additionally, direct third ventricular injection of 2-deoxy-D-glucose attenuated thefeeding behavior of chicks for a while. Overall, wespeculatethat thetechniqueis effectivefor local and/or chronic stimulation of the nucleus near the third ventricle of the chick hypothalamus, which is important for feed and energy metabolism regulation.

MISC

 137

担当経験のある科目(授業)

 3

共同研究・競争的資金等の研究課題

 6

産業財産権

 2