Curriculum Vitaes
Profile Information
- Affiliation
- Senior Assistant Professor, Faculty of Veterinary Science, School of Veterinary Medicine, Laboratory of Comparative Cellular Biology, Nippon Veterinary and Life Science University
- J-GLOBAL ID
- 200901097779808655
- researchmap Member ID
- 6000001096
Research Areas
2Education
2Papers
28-
Journal of Comparative Pathology, 197 19-22, Sep, 2022
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Archives of medical sciences. Atherosclerotic diseases, 7 e73-e77, 2022INTRODUCTION: Spontaneously hyperlipidemic (SHL) mice, a mouse strain derived from an inbred strain of Japanese wild (original)-type mice (KOR; Mus musculus molossinus), show high plasma cholesterol concentrations with disruption of the apolipoprotein E (Apoe) gene. However, the details of the Apoe gene of SHL mice have yet to be described. MATERIAL AND METHODS: The DNA sequence of the Apoe gene of SHL mice was compared to that of control KOR mice in genomic DNA and cDNA analyses. RESULTS: In the DNA analysis, a 4700-bp fragment was found to be inserted into exon 4 of the Apoe gene of SHL mice. The insertion contained two 365-bp repeats at each terminal and was flanked by a 6-bp target duplication at each side. The inserted fragment produced a frameshift of an early stop codon, resulting in a protein product that consisted of 87 amino acids in SHL mice compared to 311 amino acids in control KOR mice. CONCLUSIONS: These findings provide useful information about the molecular basis of SHL mice and related lipid disorders.
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Cancers, 12(10), Oct 14, 2020 Peer-reviewedFibroblast growth factor receptor 4 (FGFR4), one of four tyrosine kinase receptors for FGFs, is involved in diverse cellular processes. Activation of FGF19/FGFR4 signaling is closely associated with cancer development and progression. In this study, we examined the expression and roles of FGF19/FGFR4 signaling in human pancreatic ductal adenocarcinoma (PDAC). In human PDAC cases, FGFR4 expression positively correlated with larger primary tumors and more advanced stages. Among eight PDAC cell lines, FGFR4 was expressed at the highest levels in PK-1 cells, in which single-nucleotide polymorphism G388R in FGFR4 was detected. For inhibition of autocrine/paracrine FGF19/FGFR4 signaling, we used BLU9931, a highly selective FGFR4 inhibitor. Inhibition of signal transduction through ERK, AKT, and STAT3 pathways by BLU9931 reduced proliferation in FGF19/FGFR4 signaling-activated PDAC cells. By contrast, BLU9931 did not alter stemness features, including stemness marker expression, anticancer drug resistance, and sphere-forming ability. However, BLU9931 inhibited cell invasion, in part, by downregulating membrane-type matrix metalloproteinase-1 in FGF19/FGFR4 signaling-activated PDAC cells. Furthermore, downregulation of SIRT1 and SIRT6 by BLU9931 contributed to senescence induction, priming these cells for quercetin-induced death, a process termed senolysis. Thus, we propose that BLU9931 is a promising therapeutic agent in FGFR4-positive PDAC, especially when combined with senolysis (195/200).
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Journal of comparative pathology, 179 31-35, Aug, 2020Abdominal ultrasonographical and computed tomography examinations of a 12-year-old neutered female toy poodle revealed a protruding mass, approximately 2 cm in diameter, at the apex of the bladder. The mass was firm and haemorrhagic with a homogeneously brownish-yellow cut surface. Microscopically, it was unencapsulated and located in the muscle layer with invasion of the extra-muscular layer. It was composed of spindloid to oval neoplastic cells that formed irregular clefts and diffuse sheets that dissected bundles of collagen. Immunohistochemically, the neoplastic cells were positive for vimentin and lymphatic vessel endothelial hyaluronan receptor 1 antigens, but negative for cytokeratin AE1/AE3, factor VIII-related antigen, CD31, CD34, Prox-1, S100, desmin, α-smooth muscle actin and MyoD1. Negative immunolabelling for laminin antigen supported the absence of evidence of a basal lamina on ultrastructural examination. Based on these findings, this tumour was identified as a lymphangiosarcoma. To the best of our knowledge, this case is the first report of lymphangiosarcoma arising from the bladder in a dog.
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Scientific reports, 10(1) 8576-8576, May 22, 2020 Peer-reviewedThe Japanese murrelet (Synthliboramphus wumizusume) is an endangered small seabird species in Japan. Molecular sexing using PCR targeting of the gene encoding chromodomain helicase DNA-binding protein 1(CHD1) has been used for sex identification. Specifically, PCR using any of three commonly used primer sets (CHD1F/1R, 2550F/2718R and P2/P8) has permitted sexing in many bird species. CHD1F/1R and 2550F/2718R permitted molecular sexing in Japanese murrelet; however, P2/P8 did not permit. To generate a primer pair that permits efficient molecular sexing in this species, a new primer set, CHD1F1/1R1, was prepared to permit amplification of smaller products from degraded DNA samples. The electrophoretic patterns of PCR products amplified with the new primer set were easily classified as female or male. Additionally, the PCR product indicated the presence of a polymorphism in the fragment from chromosome W. The PCR fragments of long-type (WL) and short-type (WS) polymorphisms were observed only in females. When the distribution of the CHD1 gene on chromosome W of 61 female Japanese murrelet on Biroujima Island in Miyazaki Prefecture, WL and WS were observed in 90.2% and 9.8%. The DNA polymorphism is derived from the number of copies of a 32-bp-repeat unit, with WL and WS corresponding to two and one 32-bp-repeats, respectively.
Misc.
42Presentations
30Teaching Experience
14-
2022 - PresentPractice for Teaching Profession (Co-lecture) (Nippon Veterinary and Life Science University)
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2020 - PresentTheory of Life Science Research (Co-lecture) (Nippon Veterinary and Life Science University)
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2012 - PresentBiology Lab. (Nippon Veterinary and Life Science University)
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2008 - PresentMethods of Science Education Ⅳ (Nippon Veterinary and Life Science University)
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2008 - PresentMethods of Science Education Ⅱ (Nippon Veterinary and Life Science University)
Professional Memberships
7-
Jul, 2022 - Present
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Aug, 2009 - Present
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Feb, 2009 - Present
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Oct, 2005 - Present
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Sep, 2003 - Present
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Jan, 2003 - Present
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Sep, 2001 - Present
Research Projects
3-
Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018
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Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, 2013 - 2014
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Grants-in-Aid for Scientific Research(基盤研究(C)), Ministry of Education, Culture, Sports, Science and Technology, 2005 - 2007