Curriculum Vitaes
Profile Information
- Affiliation
- Nippon Veterinary and Life Science University
- J-GLOBAL ID
- 201601012964432815
- researchmap Member ID
- B000258755
Research Interests
5Research Areas
1Education
2-
Apr, 1994 - Mar, 1998
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Apr, 1988 - Mar, 1994
Papers
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Animals : an open access journal from MDPI, 12(23), Dec 2, 2022The objectives of this study were to assess the sequential dynamics of the endometrial polymorphonuclear cells (PMN) after calving by endometrial cytology, and clarify the factors that cause prolonged endometrial inflammation in lactating dairy cows. A total of 33 lactating Holstein dairy cows were used from -4 to 8 wk relative to calving (0 wk: the calving week). Endometrial samples were obtained sequentially from 2 to 8 wk. Body condition score and backfat thickness were obtained weekly from -4 to 8 wk. Blood samples collected from -4 to 8 wk were analyzed for indicators of energy status, hepatic function, systemic inflammation, and calcium. Blood amino acids were measured at 2 wk. Daily milk production was determined between 5 and 65 d postpartum. Based on the sequential cytological analysis, the endometrial inflammation threshold was set at ≥5.0% PMN, and the median wk of PMN% lower than 5.0% was 4.5 wk in this study; therefore, we classified the cows into the early group (cows with endometrial inflammation converged within 4 wk: n = 17) and the late group (cows with endometrial inflammation converged at or after 5 wk: n = 16). There were no differences in daily milk production, energy status, hepatic function, blood calcium concentration, and systemic inflammatory response. The late group had lower body condition scores and backfat thickness during the experimental period, and a higher blood concentration of 3-methyl histidine, indicating muscle breakdown, was observed in the late group at 2 wk. Our findings indicated that the lack of body fat reservation during the peripartum period and the increased muscle breakdown after calving were risk factors for prolonged endometrial inflammation.
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Japanese Journal of Veterinary Research, 66(2) 63-70, May, 2018 Peer-reviewed© 2018, Hokkaido University. All rights reserved. We investigated changes in cortisol (COR) concentration, which is well known as an index of stress in the serum of dairy cattle. The COR concentrations in serum obtained from dairy cattle were collected during practical training of first-year students on a farm attached to the Nippon Veterinary and Life Science University. Mean COR concentration in serum determined after practical training was significantly higher (P <0.001) than that in serum collected before training. Discriminant analysis was used to classify the relation between COR concentration of serum collected before and after practical training. In conclusion, the data was bipartite according to the percentage of rise (rise rate) of COR concentration. Although the percentage of the rise was more than 300% in the high-rise-rate group, there was a significant negative correlation (P < 0.05) between age and COR concertation. It was thought that the high-rise-rate group has a chance to decrease stress after more experience. In contrast, the low-rise-rate group included 3 cattle indicating high COR concentration before and after practical training. Those 3 cattle were thought to be stressed easily. It is suggested that there was individual difference to stress.
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Research in Veterinary Science, 114 163-169, Oct 1, 2017 Peer-reviewed© 2017 Anion-exchange (AEX)–high-performance liquid chromatography (HPLC) for measurement of cholesterol can be used to separate serum lipoproteins (high-density lipoprotein (HDL); low-density lipoprotein (LDL); intermediate-density lipoprotein (IDL); very-low-density lipoprotein (VLDL)) in humans. However, AEX–HPLC has not been applied in veterinary practice. We had three objectives: (i) the validation of AEX-HPLC methods including the correlation of serum cholesterol concentration in lipoprotein fraction measured by AEX–HPLC and gel permeation–HPLC (GP–HPLC) in healthy dogs and those with hypercholesterolemia was investigated; (ii) the reference intervals of lipoprotein fractions measured by AEX–HPLC from healthy dogs (n = 40) was established; (iii) lipoprotein fractions from the serum of healthy dogs (n = 12) and dogs with hypercholesterolemia (n = 23) were compared. Analytic reproducibility and precision of AEX–HPLC were acceptable. Positive correlation between serum concentrations of total cholesterol (Total-Chol), HDL cholesterol (HDL-Chol), LDL cholesterol (LDL-Chol) + IDL cholesterol (IDL-Chol), and VLDL cholesterol (VLDL-Chol) was noted for AEX–HPLC and GP–HPLC in healthy dogs and dogs with hypercholesterolemia. Reference intervals measured by AEX–HPLC for serum concentrations of Total-Chol, HDL-Chol, and LDL-Chol were determined to be 2.97–9.32, 2.79–6.57, 0.16–3.28 mmol/L (2.5–97.5% interval), respectively. Furthermore, there was significant difference in lipoprotein profiles between healthy and dogs with hypercholesterolemia. These results suggest that AEX–HPLC can be used to evaluate lipoprotein profiles in dogs and could be a new useful indicator of hyperlipidemia in dogs.
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Journal of Veterinary Medical Science, 78(8) 1277-1281, Aug, 2016 Peer-reviewed© 2016 The Japanese Society of Veterinary Science. Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as a method to obtain a feline lipoprotein profile. The lipoprotein profiles showed large and small peaks, which corresponded to high-density lipoprotein (HDL) and low-density lipoprotein (LDL), respectively, whereas very low-density lipoprotein (VLDL) and chylomicron (CM) were only marginally detected. This profile was very similar to that of dogs reported previously. Healthy cats also had a small amount of cholesterol-rich particles distinct from the normal LDL or HDL profile. There was no difference in lipoprotein profiles between the sexes, but males had a significantly larger LDL particle size (P=0.015). This study shows the feasibility of GP-HPLC for obtaining accurate lipoprotein profiles with small sample volumes and provides valuable reference data for healthy cats that should facilitate diagnoses.
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Journal of Feline Medicine and Surgery, 14(2) 147-150, Feb, 2012 Peer-reviewedA 9-year-old spayed female domestic shorthair cat presented with a skin lesion of the left tarsus. The lesion was biopsied and, based on the microscopic appearance and immunohistochemical characteristics, histiocytic sarcoma was diagnosed. Amputation was performed with improved demeanor seen postoperatively. However, between 44 and 60 days following the surgery, relapse of skin lesions appeared in multiple locations, including at the previous amputation site, and euthanasia was elected. This is the first report of a histiocytic sarcoma treated with amputation in a cat. © 2011, International Society of Feline Medicine and American Association of Feline Practitioners. All rights reserved.
Misc.
206-
ペット栄養学会誌, 14 Suppl_81-Suppl_82, 2011肥満のビーグル8頭を、&delta;-アミノレブリン酸 (以下ALA) を給与するALA給与群と、給与しない対照群の2群に分け、36日間の飼養試験を行った。結果、血清コレステロールは両群で上昇したが、ALA給与群で体重・体脂肪率の増加抑制が認められ、体内に吸収された脂肪酸がALAより産生されたATPを利用し&beta;酸化が亢進された事を示唆した。さらに、ALA給与群の糞中の飽和脂肪酸濃度が増加したため、脂肪消化率が低下する可能性が示唆され、これらによりALAが体脂肪の蓄積を抑制したものと考えられた。
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The journal of veterinary oriental medicine, 14(1) 15-19, 2006
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Journal of Pet Animal Nutrition, 9(2) 98-104, 2006This study was performed to examine the effect of the sidestream smoke exposure and the effects of the dietary antioxidant supplement for the exposition on the oxidative stress in dogs. Six healthy dogs (2 males,4 females) were exposed by sidestream smoke as secondary tobacco smoke for 30 min in a small closed chamber. Blood samples were collected for the measurement of the oxidative stress before and 0,30,60,90 and 120 min after the exposition. The oxidative stress was evaluated by d-ROMs test (reactive oxygen metabolites test). The oxidative stress was significantly higher after the exposition than before. In the meanwhile, the oxidative stress didn't elevate in dogs fed the medical food which contains rich antioxidants (vitamin C: 34mg, vitamin E: 102mg/day). Next, same exposition was performed after the administration of vitamin C: 350mg, vitamin E: 100mg, carotene: 5.77mg and coenzyme Q10: 10mg once a day for 2 weeks. The oxidative stress didn't elevate in dogs given these antioxidants. Moreover, the oxidative stress also didn't elevate in dogs after the 2-week administration of the multi vitamin-mineral food supplement prepared for dogs. The food supplement contains vitamin C: 0.132mg, vitamin E: 1.11mg, carotene: 0.225mg, coenzyme C10: 3mg and catechin: 15mg for a dog weighing 10kg per day. The results suggested that secondary tobacco smoke exposure induced an oxidative stress not only in smoking pet owners but also in pets been together, and the increase of oxidative stress was suppressed by the administration of the antioxidants. It is supposed that daily intake of antioxidants prevented the elevation of oxidative stress.
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小動物臨床, 22(2) 105-107, Mar 1, 2003
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日本獣医学会学術集会講演要旨集, 126th 199, Aug, 1998
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JOURNAL OF VETERINARY MEDICAL SCIENCE, 59(11) 1067-1069, Nov, 1997A fluorometric assay for the determination of hepatic carnitine palmitoyltransferase (CPT) activity was slightly modified for use with cattle samples. With this assay, the K-m value was 0.56 +/- 0.10 mM with respect to L-carnitine (mean +/- SD, n=4) and was 9.6 +/- 2.2 mu M (n=3) with respect to palmitoyl-CoA. The average hepatic CPT activity was 33.6 +/- 2.0 mu mol CoASH/min/g protein in 38 healthy cattle and was similar in both sexes and among breeds. Hepatic CPT activity showed no correlation with serum phospholipid, free fatty acid, triglyceride or total cholesterol concentrations.
Books and Other Publications
1Presentations
51Teaching Experience
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Apr, 2023 - PresentProductive Animal Clinical Medicine (Nippon Veterinary and Life Science University)
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2006 - Jul, 2015Veterinary Internal Medicine (Nippon Veterinary and Life Science University)
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2007 - Mar, 2011Veterinary endocrinical and metabolic diseases (Nippon Veterinary and Life Science University)