Faculty of Applied Life Science
基本情報
研究分野
1経歴
4-
2020年4月 - 現在
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2012年4月 - 2020年3月
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2011年4月 - 2012年3月
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2009年4月 - 2011年3月
学歴
3-
2008年4月 - 2011年3月
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2006年4月 - 2008年3月
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2002年4月 - 2006年3月
委員歴
4-
2020年4月 - 現在
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2013年 - 現在
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2012年4月 - 現在
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2012年4月 - 2019年3月
受賞
5論文
37-
The Journal of Poultry Science 61 2024017 2024年6月
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The Journal of Poultry Science 60(2) 2023年7月 査読有り
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Poultry Science 102(1) 102249-102249 2022年10月 査読有り
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Animals 12(11) 1434-1434 2022年6月2日 査読有りFor the conservation of endangered avian species, developing gamete preservation technologies is essential. However, studies in oocytes have not been widely conducted. In this study, assuming that the ovaries are transported to a research facility after death, we investigated the effect of ovary storage on oocytes for the purpose of cryopreserving avian female gametes by using a chicken as a model of endangered avian species. After excision, the ovaries were stored at either a low temperature (4 °C) or room temperature for 1–3 days. Ovarian follicles stored under different conditions for each period were examined by neutral red staining, histology, and gene and protein expression analysis. In addition, the pH of the storage medium after preserving the ovaries was measured. Then, ovarian tissues were vitrified to determine the cryopreservation competence. Storing the ovarian tissues at 4 °C kept the follicles viable and morphologically normal for 3 days with slow decline. In contrast, although different storage temperature did not influence follicle viability and morphology after only 1 day of storage, ovarian tissues stored at room temperature rapidly declined in structurally normal follicles, and viable follicles were rarely seen after 3 days of storage. Gene and protein expression analysis showed that apoptosis had already started on the first day, as shown by the higher expression of CASP9 under room temperature conditions. Furthermore, high expression of SOD1 and a rapid decline of pH in the storage medium under room temperature storage suggested the influence of oxidative stress associated with low pH in this condition on the follicle survivability in hen ovarian tissues. Our cryopreservation study also showed that ovarian tissues stored at 4 °C could recover after cryopreservation even after 3 days of storage. The described storage conditions and cryopreservation methods, which preserve chicken follicle survival, will lay the foundation of ovarian tissue preservation to preserve the fertility of wild female birds.
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Metabolites 12(1) 86-86 2022年1月17日 査読有り最終著者責任著者To explore metabolic characteristics during the post-hatch developmental period, metabolomic analyses of breast muscle and plasma were performed in chickens. The most significant growth-related changes in metabolite levels were observed between seven and 28 days of age. Some of these metabolites are essential nutrients or reported as growth-promoting metabolites. In the muscle, two imidazole dipeptides—carnosine and its methylated metabolite, anserine—increased with the development. These dipeptide levels may be, in part, regulated transcriptionally because in the muscle mRNA levels of carnosine synthase and carnosine methylation enzyme increased. In contrast, taurine levels in the muscle decreased. This would be substrate availability-dependent because some upstream metabolites decreased in the muscle or plasma. In branched-chain amino acid metabolism, valine, leucine, and isoleucine decreased in the muscle, while some of their downstream metabolites decreased in the plasma. The polyamines, putrescine and spermidine, decreased in the muscle. Furthermore, mRNA levels associated with insulin/insulin-like growth factor 1 signaling, which play important roles in muscle growth, increased in the muscle. These results indicate that some metabolic pathways would be important to clarify metabolic characteristics and/or growth of breast muscle during the post-hatch developmental period in chickens.
MISC
128書籍等出版物
1-
Nova Science Publishers, Inc. ISBN: 9781626184213 2013年
講演・口頭発表等
12-
AWFCJ (Animal Welfare Food Community Japan) セミナー 2022年10月29日 招待有り
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World Poultry Congress 2022, Paris, France
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67th International Congress of Meat Science and Technology. (Hybrid Congress) KRAKOW, Poland
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The 2nd International Conference on native chicken 2019 2019年7月9日
共同研究・競争的資金等の研究課題
11-
日本学術振興会 科学研究費助成事業 2023年4月 - 2026年3月
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公益財団法人 三島海雲記念財団 第60回学術研究奨励金 2022年4月 - 2023年3月
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一般財団法人 旗影会 2022年度 研究助成 2022年4月 - 2023年3月
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日本学術振興会 科学研究費助成事業 基盤研究(C) 2020年4月 - 2023年3月
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一般財団法人 旗影会 2020年度 研究助成 2020年4月