Curriculum Vitaes
Profile Information
- Affiliation
- Associate Professor, School of Veterinary Medicine, Nippon Veterinary and Life Science University
- Degree
- PhD(Mar, 1998, Hokkaido University)BSc(Mar, 1994, Rakuno Gakuen University)
- J-GLOBAL ID
- 200901085122883977
- researchmap Member ID
- 6000014205
Research Interests
11Research Areas
3Research History
4-
Apr, 1998 - Mar, 2005
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Aug, 2000 - Jul, 2002
Education
2-
Apr, 1988 - Mar, 1994
Committee Memberships
2-
Apr, 2015 - Present
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2009 - Present
Papers
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General and comparative endocrinology, 353 114520-114520, Apr 18, 2024G protein-coupled receptor 84 (GPR84) was cloned as an orphan receptor, and medium-chain fatty acids were then revealed as endogenous ligands. GPR84 is expressed in immune cells and is believed to protect liver function from lipotoxicity caused by overeating and high-fat diet intake. This study aimed to present the molecular characterization of GPR84 in domestic cats. The deduced amino acid sequence of the feline GPR84 shows high sequence homology (83-89 %) with the orthologues from other mammalians by cDNA cloning of feline GPR84. Remarkably high mRNA expression was observed in the bone marrow by Q-PCR analysis. The inhibition of intracellular cAMP concentration was observed in cells transfected with feline GPR84 and treated with medium-chain fatty acids. Immunostaining of GPR84 and free fatty acid receptor 2 (FFAR2)/GPR43 in the bone marrow, where high mRNA expression was observed, showed reactions in macrophages and myeloid cells. To clarify whether the receptor formed homo/hetero-merization, GPR84 and FFARs were analyzed using Nano-Luc binary technology and NanoLuc bioluminescence resonance energy transfer technologies, which revealed that GPR84 formed more heteromers with FFAR2 than homomers with each other. In addition, when GPR84 and FFAR2/GPR43 were cotransfected in the cell, their localization on the cell membrane was reduced compared with that when single receptors were transfected. These results indicated that GPR84 is a functional receptor protein that is expressed in cat tissues and may have a protein-protein interaction with FFAR2/GPR43 on the cell membrane.
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Journal of Food Biochemistry, Mar 21, 2022 Peer-reviewed
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Journal of pharmacological sciences, 140(1) 48-53, May, 2019 Peer-reviewed
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Journal of advanced veterinary and animal research, 6(1) 1-8, Mar, 2019 Peer-reviewed
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Journal of Pharmacological Sciences, 136(1) 26-30, Jan 1, 2018 Peer-reviewedChlorogenic acid (CGA) is a polyphenol found in coffee and medicinal herbs such as Lonicera japonica. In this study, the effect of CGA-induced relaxation on carbachol (CCh)-induced contraction of mouse urinary bladder was investigated. CGA (30–300 μg/ml) inhibited CCh- or U46619-induced contraction in a concentration-dependent manner. SQ22536 (adenylyl cyclase inhibitor) recovered CGA-induced relaxation of CCh-induced contraction however, ODQ (guanylyl cyclase inhibitor) did not have the same effect. In addition, 3-isobutyl-1-methylxanthine (IBMX) enhanced CGA-induced relaxation however, forskolin or sodium nitroprusside did not have the same effect. Moreover, Ro 20–1724, a selective phosphodiesterase (PDE) 4 inhibitor, enhanced CGA-induced relaxation, but vardenafil, a selective PDE5 inhibitor, did not have the same effect. In the presence of CCh, CGA increased cyclic adenosine monophosphate (cAMP) level, whereas SQ22536 inhibited the increase of cAMP levels. Moreover, higher cAMP levels were obtained with CGA plus IBMX treatment than the total cAMP levels obtained with separate CGA and IBMX treatments. In conclusion, these results suggest that CGA inhibited CCh-induced contraction of mouse urinary bladder by partly increasing cAMP levels via adenylyl cyclase activation.
Misc.
28-
Japanese Journal of Veterinary History, 60 9-30, Feb, 2023 Peer-reviewedLead authorCorresponding author
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Japanese Journal of Veterinary History, 57 24-41, Feb, 2020 Peer-reviewedLead authorCorresponding author
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Japanese Journal of Veterinary History, 54 60-65, Feb, 2017 Peer-reviewedInvited
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JOURNAL OF PHARMACOLOGICAL SCIENCES, 128(3) S171-S171, Jul, 2015
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ペット栄養学会誌, 15 Suppl_34-Suppl_35, 2012飢餓状態などのケトン体生成が増加する状況では肝臓での酢酸生成が増加する。特に血糖維持を糖新生に依存するネコではケトン体、酢酸生成が増加しやすいと考えられる。本研究ではネコの栄養状態および病態評価に血中酢酸濃度を利用する目的で酢酸測定法を検討し、健常ネコでの標準値を測定するとともに糖尿病ネコでの変動を調べた。健常ネコの血中酢酸濃度は 0.71 ± 0.23mM であり、雌雄間で有意な差が認められた(p<0.01)。また糖尿病 8 症例の平均は 0.91 ± 0.34mM であり、健常ネコよりも高値を示す傾向が見られた。
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OBESITY RESEARCH, 12 A125-A125, Oct, 2004
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日本生態学会誌, 51(2) 143-143, 2001 Peer-reviewed
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REVUE DE MEDECINE VETERINAIRE, 151(7) 769-769, Jul, 2000 Lead author
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最新医学, 52(6) 1093-1100, Jun, 1997
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肥満研究 : 日本肥満学会誌 = Journal of Japan Society for the Study of Obesity, 3(1) 60-62, May 31, 1997
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実験医学, 14(16) 2222-2227, Oct, 1996
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動物臨床医学, 5(2) 11-14, Sep, 1996
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診断と治療, 84(6) 987-992, Jun, 1996
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家畜生化学, 33(1) 13-18, Mar, 1996
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北海道獣医師会雑誌, 39(9) 178-180, Sep, 1995
Books and Other Publications
4Presentations
48Teaching Experience
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Apr, 2021 - Present獣医臨床栄養学(基礎) (日本獣医生命科学大学)
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2021 - Present獣医生化学実習 (日本獣医生命科学大学)
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Sep, 2020 - PresentHistory of science (Nippon Veterinary and Life Science University)
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Apr, 2020 - Present生命科学研究論 (日本獣医生命科学大学)
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Sep, 2016 - Present獣医史学 (日本獣医生命科学大学)
Professional Memberships
7Research Projects
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科学研究費助成事業, 日本学術振興会, Apr, 2024 - Mar, 2027
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Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
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科学研究費補助金(研究成果公開促進費)(研究成果公開発表(B)(ひらめき☆ときめきサイエンス~ようこそ大学の研究室へ~KAKENHI)), 文部科学省, Apr, 2019 - Mar, 2020
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Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Oct, 2016 - Mar, 2019
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研究助成事業, 山崎香辛料振興財団, Apr, 2016 - Mar, 2017