獣医学科

佐々木 典康

ササキ ノリヤス  (Noriyasu Sasaki)

基本情報

所属
日本獣医生命科学大学 獣医学科 獣医生化学研究室 准教授
学位
博士(獣医学)(1998年3月 北海道大学)
学士(獣医学)(1994年3月 酪農学園大学)

J-GLOBAL ID
200901085122883977
researchmap会員ID
6000014205

委員歴

 2

論文

 26
  • Ichiro Yamamoto, Masaki Michishita, Koki Fujita, Tamami Sakai, Noriyasu Sasaki, Koh Kawasumi
    General and comparative endocrinology 353 114520-114520 2024年4月18日  査読有り
    G protein-coupled receptor 84 (GPR84) was cloned as an orphan receptor, and medium-chain fatty acids were then revealed as endogenous ligands. GPR84 is expressed in immune cells and is believed to protect liver function from lipotoxicity caused by overeating and high-fat diet intake. This study aimed to present the molecular characterization of GPR84 in domestic cats. The deduced amino acid sequence of the feline GPR84 shows high sequence homology (83-89 %) with the orthologues from other mammalians by cDNA cloning of feline GPR84. Remarkably high mRNA expression was observed in the bone marrow by Q-PCR analysis. The inhibition of intracellular cAMP concentration was observed in cells transfected with feline GPR84 and treated with medium-chain fatty acids. Immunostaining of GPR84 and free fatty acid receptor 2 (FFAR2)/GPR43 in the bone marrow, where high mRNA expression was observed, showed reactions in macrophages and myeloid cells. To clarify whether the receptor formed homo/hetero-merization, GPR84 and FFARs were analyzed using Nano-Luc binary technology and NanoLuc bioluminescence resonance energy transfer technologies, which revealed that GPR84 formed more heteromers with FFAR2 than homomers with each other. In addition, when GPR84 and FFAR2/GPR43 were cotransfected in the cell, their localization on the cell membrane was reduced compared with that when single receptors were transfected. These results indicated that GPR84 is a functional receptor protein that is expressed in cat tissues and may have a protein-protein interaction with FFAR2/GPR43 on the cell membrane.
  • Hisako Kaneda, Misa Hori, Haruka Shinomiya, Ayaka Nakajima, Shingo Yamazaki, Noriyasu Sasaki, Tsuyoshi Sato, Takeharu Kaneda
    Journal of Food Biochemistry 2022年3月21日  査読有り
  • Kaneda H, Otomo R, Sasaki N, Omi T, Sato T, Kaneda T
    Journal of pharmacological sciences 140(1) 48-53 2019年5月  査読有り
  • Saga S, Sasaki N, Arai T
    Journal of advanced veterinary and animal research 6(1) 1-8 2019年3月  査読有り
  • Takeharu Kaneda, Noriyasu Sasaki, Norimoto Urakawa, Kazumasa Shimizu
    Journal of Pharmacological Sciences 136(1) 26-30 2018年1月1日  査読有り
    Chlorogenic acid (CGA) is a polyphenol found in coffee and medicinal herbs such as Lonicera japonica. In this study, the effect of CGA-induced relaxation on carbachol (CCh)-induced contraction of mouse urinary bladder was investigated. CGA (30–300 μg/ml) inhibited CCh- or U46619-induced contraction in a concentration-dependent manner. SQ22536 (adenylyl cyclase inhibitor) recovered CGA-induced relaxation of CCh-induced contraction however, ODQ (guanylyl cyclase inhibitor) did not have the same effect. In addition, 3-isobutyl-1-methylxanthine (IBMX) enhanced CGA-induced relaxation however, forskolin or sodium nitroprusside did not have the same effect. Moreover, Ro 20–1724, a selective phosphodiesterase (PDE) 4 inhibitor, enhanced CGA-induced relaxation, but vardenafil, a selective PDE5 inhibitor, did not have the same effect. In the presence of CCh, CGA increased cyclic adenosine monophosphate (cAMP) level, whereas SQ22536 inhibited the increase of cAMP levels. Moreover, higher cAMP levels were obtained with CGA plus IBMX treatment than the total cAMP levels obtained with separate CGA and IBMX treatments. In conclusion, these results suggest that CGA inhibited CCh-induced contraction of mouse urinary bladder by partly increasing cAMP levels via adenylyl cyclase activation.

MISC

 28

書籍等出版物

 4
  • 丸山マサ美, 木村利人, 足立智孝, 宮坂義浩, 吉住朋晴, 佐々木典康, 三成寿作, 瀬戸山 晃一 (担当:分担執筆, 範囲:第5章 医学・生命科学研究における動物実験の倫理)
    大学教育出版 2024年6月 (ISBN: 9784866923031)
  • 横田 博, 木村和弘, 志水泰武 (担当:分担執筆, 範囲:17 組換えDNA技術)
    朝倉書店 2016年4月 (ISBN: 9784254460353)
  • アンドリュー・ガーディナー, 多川政弘 (担当:共訳, 範囲:12章雌の生殖器,13章内分泌疾患)
    インターズー 2005年10月 (ISBN: 489995347X)
  • 斉藤 昌之, 鈴木 嘉彦, 横田 博 (担当:分担執筆, 範囲:19 組換えDNA技術)
    朝倉書店 2005年 (ISBN: 9784254460254)

講演・口頭発表等

 48

担当経験のある科目(授業)

 9

共同研究・競争的資金等の研究課題

 10

産業財産権

 1

社会貢献活動

 7

メディア報道

 2