基本情報
- 所属
- 日本獣医生命科学大学 獣医学科 獣医生化学研究室 准教授
- 学位
- 博士(獣医学)(1998年3月 北海道大学)学士(獣医学)(1994年3月 酪農学園大学)
- J-GLOBAL ID
- 200901085122883977
- researchmap会員ID
- 6000014205
経歴
4-
2014年4月 - 現在
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2005年4月 - 2014年3月
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1998年4月 - 2005年3月
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2000年8月 - 2002年7月
学歴
2-
1994年4月 - 1998年3月
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1988年4月 - 1994年3月
委員歴
2-
2015年4月 - 現在
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2009年 - 現在
論文
26-
General and comparative endocrinology 353 114520-114520 2024年4月18日 査読有りG protein-coupled receptor 84 (GPR84) was cloned as an orphan receptor, and medium-chain fatty acids were then revealed as endogenous ligands. GPR84 is expressed in immune cells and is believed to protect liver function from lipotoxicity caused by overeating and high-fat diet intake. This study aimed to present the molecular characterization of GPR84 in domestic cats. The deduced amino acid sequence of the feline GPR84 shows high sequence homology (83-89 %) with the orthologues from other mammalians by cDNA cloning of feline GPR84. Remarkably high mRNA expression was observed in the bone marrow by Q-PCR analysis. The inhibition of intracellular cAMP concentration was observed in cells transfected with feline GPR84 and treated with medium-chain fatty acids. Immunostaining of GPR84 and free fatty acid receptor 2 (FFAR2)/GPR43 in the bone marrow, where high mRNA expression was observed, showed reactions in macrophages and myeloid cells. To clarify whether the receptor formed homo/hetero-merization, GPR84 and FFARs were analyzed using Nano-Luc binary technology and NanoLuc bioluminescence resonance energy transfer technologies, which revealed that GPR84 formed more heteromers with FFAR2 than homomers with each other. In addition, when GPR84 and FFAR2/GPR43 were cotransfected in the cell, their localization on the cell membrane was reduced compared with that when single receptors were transfected. These results indicated that GPR84 is a functional receptor protein that is expressed in cat tissues and may have a protein-protein interaction with FFAR2/GPR43 on the cell membrane.
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Journal of pharmacological sciences 140(1) 48-53 2019年5月 査読有り
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Journal of advanced veterinary and animal research 6(1) 1-8 2019年3月 査読有り
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Journal of Pharmacological Sciences 136(1) 26-30 2018年1月1日 査読有りChlorogenic acid (CGA) is a polyphenol found in coffee and medicinal herbs such as Lonicera japonica. In this study, the effect of CGA-induced relaxation on carbachol (CCh)-induced contraction of mouse urinary bladder was investigated. CGA (30–300 μg/ml) inhibited CCh- or U46619-induced contraction in a concentration-dependent manner. SQ22536 (adenylyl cyclase inhibitor) recovered CGA-induced relaxation of CCh-induced contraction however, ODQ (guanylyl cyclase inhibitor) did not have the same effect. In addition, 3-isobutyl-1-methylxanthine (IBMX) enhanced CGA-induced relaxation however, forskolin or sodium nitroprusside did not have the same effect. Moreover, Ro 20–1724, a selective phosphodiesterase (PDE) 4 inhibitor, enhanced CGA-induced relaxation, but vardenafil, a selective PDE5 inhibitor, did not have the same effect. In the presence of CCh, CGA increased cyclic adenosine monophosphate (cAMP) level, whereas SQ22536 inhibited the increase of cAMP levels. Moreover, higher cAMP levels were obtained with CGA plus IBMX treatment than the total cAMP levels obtained with separate CGA and IBMX treatments. In conclusion, these results suggest that CGA inhibited CCh-induced contraction of mouse urinary bladder by partly increasing cAMP levels via adenylyl cyclase activation.
MISC
28書籍等出版物
4講演・口頭発表等
48担当経験のある科目(授業)
9共同研究・競争的資金等の研究課題
10-
日本学術振興会 科学研究費助成事業 2024年4月 - 2027年3月
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日本学術振興会 科学研究費助成事業 基盤研究(C) 2020年4月 - 2023年3月
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文部科学省 科学研究費補助金(研究成果公開促進費)(研究成果公開発表(B)(ひらめき☆ときめきサイエンス~ようこそ大学の研究室へ~KAKENHI)) 2019年4月 - 2020年3月
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日本学術振興会 科学研究費助成事業 基盤研究(C) 2016年10月 - 2019年3月
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山崎香辛料振興財団 研究助成事業 2016年4月 - 2017年3月