School of Veterinary Medicine

Shuichi Tsuchida

  (土田 修一)

Profile Information

Affiliation
Professor, Nippon Veterinary and Life Science University
Degree
(BLANK)(Jichi Medical University)
(BLANK)(Nippon Veterinary and Life Science University)

J-GLOBAL ID
200901017121997663
researchmap Member ID
1000063288

Papers

 40
  • 近江 俊徳, 宇埜 友美子, 中村 知尋, 落合 和彦, 青木 博史, 宇田川 智野, 浅野 潤三, 土田 修一
    日本獣医学会学術集会講演要旨集, 164回 [HSO-62], Sep, 2021  
  • 近江 俊徳, 宇埜 友美子, 中村 知尋, 落合 和彦, 青木 博史, 宇田川 智野, 浅野 潤三, 土田 修一
    日本獣医学会学術集会講演要旨集, 164回 [HSO-62], Sep, 2021  
  • Hitoshi Hatakeyama, Yutaka Nakamura, Takahiro Konaka, Shin Nishida, Wannapimol Kriangwanich, Kazuyoshi Uematsu, Shuichi Tsuchida
    Scientific reports, 10(1) 8576-8576, May 22, 2020  Peer-reviewed
    The Japanese murrelet (Synthliboramphus wumizusume) is an endangered small seabird species in Japan. Molecular sexing using PCR targeting of the gene encoding chromodomain helicase DNA-binding protein 1(CHD1) has been used for sex identification. Specifically, PCR using any of three commonly used primer sets (CHD1F/1R, 2550F/2718R and P2/P8) has permitted sexing in many bird species. CHD1F/1R and 2550F/2718R permitted molecular sexing in Japanese murrelet; however, P2/P8 did not permit. To generate a primer pair that permits efficient molecular sexing in this species, a new primer set, CHD1F1/1R1, was prepared to permit amplification of smaller products from degraded DNA samples. The electrophoretic patterns of PCR products amplified with the new primer set were easily classified as female or male. Additionally, the PCR product indicated the presence of a polymorphism in the fragment from chromosome W. The PCR fragments of long-type (WL) and short-type (WS) polymorphisms were observed only in females. When the distribution of the CHD1 gene on chromosome W of 61 female Japanese murrelet on Biroujima Island in Miyazaki Prefecture, WL and WS were observed in 90.2% and 9.8%. The DNA polymorphism is derived from the number of copies of a 32-bp-repeat unit, with WL and WS corresponding to two and one 32-bp-repeats, respectively.
  • Toshinori Omi, Sachie Nakiri, Setsuko Nakanishi, Naomi Ishii, Taiki Uno, Fumiharu Konno, Takeshi Inagaki, Atsushi Sakamoto, Masayuki Shito, Chihiro Udagawa, Naomi Tada, Kazuhiko Ochiai, Takuya Kato, Yoshi Kawamoto, Shuichi Tsuchida, Shin-Ichi Hayama
    BMC research notes, 13(1) 121-121, Mar 2, 2020  Peer-reviewed
    OBJECTIVES: Following the massive earthquake that struck eastern Japan on March 11, 2011, a large amount of radioactive material was released into the environment from the damaged reactor of the Fukushima Daiichi Nuclear Power Plant (FDNPP). After the FDNPP accident, radiocaesium was first detected in muscle samples from wild Japanese monkeys exposed to radioactive materials, and haematologic effects, changes in head size, and delayed body weight gain were also reported, but little is known about the distribution of 137Cs in the organs and tissues of wild Japanese monkeys. RESULTS: We detected the 137Cs in various organ and tissue samples of 10 wild Japanese monkeys inhabiting the forested areas of Fukushima City that were captured between July and August 2012. Among muscle, brain, heart, kidney, liver, lung, and spleen, muscle exhibited the highest and the brain the lowest 137Cs concentration. The concentration (mean ± SD) of 137Cs in muscle, brain, heart, kidney, liver, lung, and spleen was 77 ± 66, 26 ± 22, 41 ± 35, 49 ± 41, 41 ± 38, 53 ± 41, and 53 ± 51 Bq/kg, respectively. These results can help us understand the biological effects of long-term internal radiation exposure in non-human primates.
  • 赤坂 直比古, 畠山 仁, 土田 修一
    日本比較臨床医学会誌, 26 35-35, Dec, 2019  

Misc.

 57

Teaching Experience

 4

Research Projects

 20