Curriculum Vitaes
Profile Information
- Affiliation
- Faculty of Veterinary Science, School of Veterinary Nursing and Technology, Nippon Veterinary and Life Science University
- Degree
- Doctor of veterinary science (#223)(Mar, 1989, Nippon Veterinary and Life Science University)
- Other name(s) (e.g. nickname)
- Yoji Hakamata
- J-GLOBAL ID
- 200901087420386440
- researchmap Member ID
- 1000063736
Research Interests
4Research Areas
3Research History
3-
Apr, 2020 - Present
Papers
67-
Nanomaterials, 14(17) 1440-1440, Sep 3, 2024 Peer-reviewedHemozoin (Hz) is a heme crystal produced during malaria infection that stimulates immune cells, leading to the production of cytokines and chemokines. The immunostimulatory action of Hz has previously been applied in the development of alternative adjuvants. Crystallization of hemin is a chemical approach for producing Hz. Here, we focused on an enzymatic production method for Hz using the heme detoxification protein (HDP), which catalyzes heme dimer formation from hemin in Plasmodium. We examined the immunostimulatory effects of an enzymatically synthesized analog of Hz (esHz) produced by recombinant Plasmodium falciparum HDP. Enzymatically synthesized Hz stimulates a macrophage cell line and human peripheral mononuclear cells, leading to the production of interleukin (IL)-6 and IL-12p40. In mice, subcutaneous administration of esHz together with an antigen, ovalbumin (OVA), increased the OVA-specific immunoglobulin (Ig) G2c isotype level in the serum, whereas OVA-specific IgG1 was not induced. Our findings suggest that esHz is a useful Th-1 cell adjuvant.
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Acta Cirúrgica Brasileira, 39, 2024 Peer-reviewed
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Cell transplantation, 33 9636897231224174-9636897231224174, 2024 Peer-reviewedFireflies produce light through luciferase-catalyzed reactions involving luciferin, oxygen, and adenosine triphosphate, distinct from other luminescent organisms. This unique feature has revolutionized molecular biology and physiology, serving as a valuable tool for cellular research. Luciferase-based bioluminescent imaging enabled the creation of transgenic animals, such as Firefly Rats. Firefly Rats, created in 2006, ubiquitously express luciferase and have become a critical asset in scientific investigations. These rats have significantly contributed to transplantation and tissue engineering studies. Their low immunogenicity reduces graft rejection risk, making them ideal for long-term tracking of organ/tissue/cellular engraftments. Importantly, in the islet transplantation setting, the ubiquitous luciferase expression in these rats does not alter islet morphology or function, ensuring accurate assessments of engrafted islets. Firefly Rats have illuminated the path of transplantation research worldwide for over a decade and continue accelerating scientific advancements in many fields.
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Acta cirurgica brasileira, 39 e391324, 2024 Peer-reviewedCorresponding authorPURPOSE: To develop a new 4/6 infarct nephrectomy (INx) model rat mimicking moderate chronic kidney disease (CKD) and to evaluate its application. METHODS: We modified the conventional 5/6 INx rat model to create the 4/6 INx model by ligating the renal artery branch to induce infarction of one-third of the left kidney after right kidney removal and compared biochemically and histologically both models. To demonstrate the application of the 4/6 INx model, the effects of a supplementary compound containing calcium carbonate, chitosan, palm shell activated charcoal etc., that is effective for both CKD and its complications, were compared between both models. RESULTS: Impairment of renal function in the 4/6 INx group was significantly more moderate than in the 5/6 INx group (P < 0.05). The 4/6 INx group showed less histological damage in kidney than in the 5/6 INx group. The supplementary compound did not improve CKD in the 5/6 INx group, but ameliorated elevation of blood urea nitrogen in the 4/6 INx group. CONCLUSIONS: We developed the 4/6 INx model, which is more moderate than the conventional 5/6 INx model. This model could potentially demonstrate the effectiveness of drugs and supplements intended to prevent CKD and its progression.
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Metabolites, Oct 28, 2023 Peer-reviewed
Misc.
145-
Neuropathology : official journal the Japanese Society of Neuropathology, 22 146-146, May 1, 2002
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The Journal of Reproduction and Development, 47(Suppl.) a40-a40, Dec, 2001
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今日の移植, 14(6) 809-810, Nov, 2001タクロリムス及びミゾリビンを併用する際の至適投与時刻を検討し,臨床における免疫抑制薬の時間治療の為の基礎検討を目的とし,併用における時間薬理学的検討をラット新生仔小腸移植モデルを用いて行った.ミゾリビンは,20と10mg/kgの2種で行った.タクロリムス4,ミゾリビン20mg/kg・朝10時に投与群(A群),タクロリムスを10時,ミゾリビンを22時に投与群(B群),タクロリムスを22時,ミゾリビンを10時に投与群(C群),タクロリムスとミゾリビンを22時に投与群(D群)とした.ミゾリビンを20mg投与した,A,D群は全例死亡した.C群は5例中4例,B群も5例中3例が9日以内に死亡した.10mg/kgに減量して同様に実験を行い,A',B',C',D'群とした.全例7日以上生存した.体重減少は20mg/kg投与群と比べ軽度であった.B'群が最も体重減少が軽度であり,毒性軽減の効果が期待できた
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日本消化器外科学会雑誌, 34(7) 889-889, Jul, 2001
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移植, 35(総会臨時) 209-209, Sep, 2000
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Neuropathology : official journal the Japanese Society of Neuropathology, 20 222-222, Jun 1, 2000
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脳循環代謝, 12(1) 46-47, Apr, 2000一過性虚血後脳各領域のエネルギー代謝障害に並行して梗塞は出現し,速度は領域により1日から4日迄の差が認められた.虚血後のmRNA transcriptionの障害はimmediately early geneとheat shock proteinで領域による差が認められた
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NEUROPATHOLOGY, 18(Suppl.) 241-241, May, 1998
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Japanese circulation journal, 62 567-567, Feb 28, 1998
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NEUROPATHOLOGY, 17(Suppl.) 230-230, May, 1997
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Neuropathology : official journal the Japanese Society of Neuropathology, 16 188-188, May 1, 1996
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Neuropathology : official journal the Japanese Society of Neuropathology, 15 74-74, Jun 1, 1995
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Congenital Anomalies, 31(4) 305-314, 1991Abstract Bilateral hypoplastic kidneys have been found to associate with the male hypogonadism rat (hgn/hgn). The hypoplastic kidney was persistent to the male homozygote for hgn and it was also seen in some females with unknown genotype. The affected kidney was apparently smaller in size than phenotypically normal one. When females with hypoplastic kidney were mated to proven heterozygous males for hypogonadism (hgn/+), the ratio of the affected testis to phenotypically normal one was 32: 20 in the Fl generation. This segregation ratio did not deviate siginificantly from the 1: 1 ratio expected from the hypothesis that the genotype of females with hypoplastic kidney in parent generation for hypogonadism would consist of hgn/hgn alone. The ratio expected from the other hypotheses was denied statistically. The ratio of the affected kidney to phenotypically normal one in both sexs of the Fi was 53: 50, fitting to 1: 1 hypothesis for a single autosomal recessive trait. There was neither a case showing the phenotypically normal kidney with the affected testis, nor the hypoplastic kidney with the phenotypically normal testis. The results suggest that the gene responsible for the hypoplastic kidney and the gene for hypogonadism would be identical or both genes reside in the close vicinity in a chromosome. The results also suggest that the homozygotes for hgn can be selected by the presence of hypoplastic kidney. Copyright © 1991, Wiley Blackwell. All rights reserved
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日本獣医学会学術集会講演要旨集, 105回 92-92, Apr, 1988
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TERATOLOGY, 36(3) 465-465, Dec, 1987
Books and Other Publications
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Maturation Phenomenon in Cerebrak Ischemia (]G0004[)., 2001
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Ischemic Blood Flow in the Brain., 2001
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Maturation Phenomenon in Cerebral Ischemia (]G0003[), 1999
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Maturation Phenomenon in Cerebral Ischemia (]G0003[), 1999
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Maturation Phemonenon in Cerebral Ischemia (]G0003[), 1999
Presentations
16Works
2Research Projects
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Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
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Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015
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科研費 一般研究(C), 文部省, Apr, 2012 - Mar, 2014
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Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2004 - 2005
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科学研究費助成事業 基盤研究(C), 日本学術振興会, 2002 - 2003