Curriculum Vitaes
Profile Information
- Affiliation
- Faculty of Veterinary Science, School of Veterinary Nursing and Technology, Nippon Veterinary and Life Science University
- Degree
- Doctor of veterinary science (#223)(Mar, 1989, Nippon Veterinary and Life Science University)
- Other name(s) (e.g. nickname)
- Yoji Hakamata
- J-GLOBAL ID
- 200901087420386440
- researchmap Member ID
- 1000063736
Research Interests
4Research Areas
3Research History
3-
Apr, 2020 - Present
Papers
67-
Nanomaterials, 14(17) 1440-1440, Sep 3, 2024 Peer-reviewedHemozoin (Hz) is a heme crystal produced during malaria infection that stimulates immune cells, leading to the production of cytokines and chemokines. The immunostimulatory action of Hz has previously been applied in the development of alternative adjuvants. Crystallization of hemin is a chemical approach for producing Hz. Here, we focused on an enzymatic production method for Hz using the heme detoxification protein (HDP), which catalyzes heme dimer formation from hemin in Plasmodium. We examined the immunostimulatory effects of an enzymatically synthesized analog of Hz (esHz) produced by recombinant Plasmodium falciparum HDP. Enzymatically synthesized Hz stimulates a macrophage cell line and human peripheral mononuclear cells, leading to the production of interleukin (IL)-6 and IL-12p40. In mice, subcutaneous administration of esHz together with an antigen, ovalbumin (OVA), increased the OVA-specific immunoglobulin (Ig) G2c isotype level in the serum, whereas OVA-specific IgG1 was not induced. Our findings suggest that esHz is a useful Th-1 cell adjuvant.
-
Acta Cirúrgica Brasileira, 39, 2024 Peer-reviewed
-
Cell transplantation, 33 9636897231224174-9636897231224174, 2024 Peer-reviewedFireflies produce light through luciferase-catalyzed reactions involving luciferin, oxygen, and adenosine triphosphate, distinct from other luminescent organisms. This unique feature has revolutionized molecular biology and physiology, serving as a valuable tool for cellular research. Luciferase-based bioluminescent imaging enabled the creation of transgenic animals, such as Firefly Rats. Firefly Rats, created in 2006, ubiquitously express luciferase and have become a critical asset in scientific investigations. These rats have significantly contributed to transplantation and tissue engineering studies. Their low immunogenicity reduces graft rejection risk, making them ideal for long-term tracking of organ/tissue/cellular engraftments. Importantly, in the islet transplantation setting, the ubiquitous luciferase expression in these rats does not alter islet morphology or function, ensuring accurate assessments of engrafted islets. Firefly Rats have illuminated the path of transplantation research worldwide for over a decade and continue accelerating scientific advancements in many fields.
-
Acta cirurgica brasileira, 39 e391324, 2024 Peer-reviewedCorresponding authorPURPOSE: To develop a new 4/6 infarct nephrectomy (INx) model rat mimicking moderate chronic kidney disease (CKD) and to evaluate its application. METHODS: We modified the conventional 5/6 INx rat model to create the 4/6 INx model by ligating the renal artery branch to induce infarction of one-third of the left kidney after right kidney removal and compared biochemically and histologically both models. To demonstrate the application of the 4/6 INx model, the effects of a supplementary compound containing calcium carbonate, chitosan, palm shell activated charcoal etc., that is effective for both CKD and its complications, were compared between both models. RESULTS: Impairment of renal function in the 4/6 INx group was significantly more moderate than in the 5/6 INx group (P < 0.05). The 4/6 INx group showed less histological damage in kidney than in the 5/6 INx group. The supplementary compound did not improve CKD in the 5/6 INx group, but ameliorated elevation of blood urea nitrogen in the 4/6 INx group. CONCLUSIONS: We developed the 4/6 INx model, which is more moderate than the conventional 5/6 INx model. This model could potentially demonstrate the effectiveness of drugs and supplements intended to prevent CKD and its progression.
-
Metabolites, Oct 28, 2023 Peer-reviewed
Misc.
145Books and Other Publications
7-
Maturation Phenomenon in Cerebrak Ischemia (]G0004[)., 2001
-
Ischemic Blood Flow in the Brain., 2001
-
Maturation Phenomenon in Cerebral Ischemia (]G0003[), 1999
-
Maturation Phenomenon in Cerebral Ischemia (]G0003[), 1999
-
Maturation Phemonenon in Cerebral Ischemia (]G0003[), 1999
Presentations
17-
Experimental Animals, Oct, 2016肝臓の薬物代謝酵素シトクロムP450(CYP)の一部は性的二形性を示し、薬物の効果や副作用の性差の原因として臨床上重要な問題となっている。ラットにおいてCYPの性的二形性は下垂体成長ホルモン(GH)の分泌パターンの性差に強く依存するとされているが、詳細なメカニズムは明らかでない。我々は、以前にマウスアルブミンエンハンサー/プロモーターの制御下で、肝細胞特異的に蛍光タンパク質DsRed2遺伝子を発現するトランスジェニック(Alb-DsRed2 Tg)ラットを作製し、肝DsRed2発現が成熟雄ラットに限定される性的二形を示すことを報告した。本研究では、GH分泌パターンに依存する内因性CYP発現と性的二形性DsRed2発現の関連性を検討するために、本ラットのGH分泌パターンを雌雄で逆転させ、肝CYPおよびDsRed2発現を比較した。生後1日目に雌雄Alb-DsRed2 Tgラットの性腺を摘出し、雌には同時にテストステロンプロピオネートを投与した。従来の報告と一致して、実験的にGH分泌パターンを雌雄で逆転させるとCYPの性的二形性発現が逆転し、それと同期して本ラットのDsRed2発現の性的二形性が逆転した。肝DsRed2発現は性的二形CYP発現と強く相関し、本ラットはGH分泌パターンに依存する性的二形CYP発現の変化を簡便に評価できる有用なツールであることが明らかとなった。(著者抄録)
Works
2Research Projects
10-
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
-
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015
-
科研費 一般研究(C), 文部省, Apr, 2012 - Mar, 2014
-
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2004 - 2005
-
科学研究費助成事業 基盤研究(C), 日本学術振興会, 2002 - 2003