獣医保健看護学科

袴田 陽二

Yoji Hakamata

基本情報

所属
日本獣医生命科学大学 獣医学部 獣医保健看護学科
学位
獣医学博士(1989年3月 日本獣医畜産大学)

通称等の別名
袴田陽二
J-GLOBAL ID
200901087420386440
researchmap会員ID
1000063736

論文

 67
  • Kazuaki Hoshi, Anh Thi Tram Tu, Miwako Shobo, Karin Kettisen, Lei Ye, Leif Bülow, Yoji Hakamata, Tetsuya Furuya, Ryutaro Asano, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode, Tomohiko Yamazaki
    Nanomaterials 14(17) 1440-1440 2024年9月3日  査読有り
    Hemozoin (Hz) is a heme crystal produced during malaria infection that stimulates immune cells, leading to the production of cytokines and chemokines. The immunostimulatory action of Hz has previously been applied in the development of alternative adjuvants. Crystallization of hemin is a chemical approach for producing Hz. Here, we focused on an enzymatic production method for Hz using the heme detoxification protein (HDP), which catalyzes heme dimer formation from hemin in Plasmodium. We examined the immunostimulatory effects of an enzymatically synthesized analog of Hz (esHz) produced by recombinant Plasmodium falciparum HDP. Enzymatically synthesized Hz stimulates a macrophage cell line and human peripheral mononuclear cells, leading to the production of interleukin (IL)-6 and IL-12p40. In mice, subcutaneous administration of esHz together with an antigen, ovalbumin (OVA), increased the OVA-specific immunoglobulin (Ig) G2c isotype level in the serum, whereas OVA-specific IgG1 was not induced. Our findings suggest that esHz is a useful Th-1 cell adjuvant.
  • Eri Katsumata, Abhishek Kumar Ranjan, Yoshihiko Tashima, Takayuki Takahata, Toshiyuki Sato, Motoaki Kobayashi, Masami Ishii, Toyomi Takahashi, Asahi Oda, Momoko Hirano, Yoji Hakamata, Kazuhisa Sugai, Eiji Kobayashi
    Acta Cirúrgica Brasileira 39 2024年  査読有り
  • Eiji Kobayashi, Yoji Hakamata, Shin Enosawa, Kuang-Ming Shang, Hirotake Komatsu
    Cell transplantation 33 9636897231224174-9636897231224174 2024年  査読有り
    Fireflies produce light through luciferase-catalyzed reactions involving luciferin, oxygen, and adenosine triphosphate, distinct from other luminescent organisms. This unique feature has revolutionized molecular biology and physiology, serving as a valuable tool for cellular research. Luciferase-based bioluminescent imaging enabled the creation of transgenic animals, such as Firefly Rats. Firefly Rats, created in 2006, ubiquitously express luciferase and have become a critical asset in scientific investigations. These rats have significantly contributed to transplantation and tissue engineering studies. Their low immunogenicity reduces graft rejection risk, making them ideal for long-term tracking of organ/tissue/cellular engraftments. Importantly, in the islet transplantation setting, the ubiquitous luciferase expression in these rats does not alter islet morphology or function, ensuring accurate assessments of engrafted islets. Firefly Rats have illuminated the path of transplantation research worldwide for over a decade and continue accelerating scientific advancements in many fields.
  • Kazuhisa Sugai, Momoko Hirano, Asahi Oda, Masahiko Fujisawa, Saori Shono, Katsumi Ishioka, Tomoyoshi Tamura, Yoshinori Katsumata, Motoaki Sano, Eiji Kobayashi, Yoji Hakamata
    Acta cirurgica brasileira 39 e391324 2024年  査読有り責任著者
    PURPOSE: To develop a new 4/6 infarct nephrectomy (INx) model rat mimicking moderate chronic kidney disease (CKD) and to evaluate its application. METHODS: We modified the conventional 5/6 INx rat model to create the 4/6 INx model by ligating the renal artery branch to induce infarction of one-third of the left kidney after right kidney removal and compared biochemically and histologically both models. To demonstrate the application of the 4/6 INx model, the effects of a supplementary compound containing calcium carbonate, chitosan, palm shell activated charcoal etc., that is effective for both CKD and its complications, were compared between both models. RESULTS: Impairment of renal function in the 4/6 INx group was significantly more moderate than in the 5/6 INx group (P < 0.05). The 4/6 INx group showed less histological damage in kidney than in the 5/6 INx group. The supplementary compound did not improve CKD in the 5/6 INx group, but ameliorated elevation of blood urea nitrogen in the 4/6 INx group. CONCLUSIONS: We developed the 4/6 INx model, which is more moderate than the conventional 5/6 INx model. This model could potentially demonstrate the effectiveness of drugs and supplements intended to prevent CKD and its progression.
  • Asahi Oda, Yoji Hakamata, Eiji Kobayashi
    Metabolites 2023年10月28日  査読有り

MISC

 145

講演・口頭発表等

 16

共同研究・競争的資金等の研究課題

 10