研究者業績

藤田 道郎

フジタ ミチオ  (Michio Fujita)

基本情報

所属
日本獣医生命科学大学 獣医放射線学教室 教授
学位
獣医学博士(日本獣医生命科学大学)

J-GLOBAL ID
200901042480063069
researchmap会員ID
1000093613

論文

 76
  • Aki Fujiwara-Igarashi, Takafumi Ohshima, Ryusei Kojima, Michio Fujita, Yuta Nakazawa
    Veterinary medicine and science 10(3) e1456 2024年5月  
    BACKGROUND: Few epidemiological studies on respiratory medicine and the relationship between clinical signs and various respiratory diseases in cats have been reported. OBJECTIVES: This retrospective study aimed to investigate the prevalence and breed predisposition to feline respiratory diseases in Japan and determine the association between clinical signs, duration and type of respiratory diseases. METHODS: The medical records of cats with feline respiratory diseases were examined to obtain information on age, sex, breed, final diagnosis, clinical signs and duration. The odds ratios (ORs) and 95% confidence intervals were calculated to evaluate breed predispositions. Mann-Whitney U, Kruskal-Wallis and Dunn's tests were used to assess the duration of clinical signs. RESULTS: This study included 540 cats with 615 respiratory diagnoses. The American Shorthair breed was predisposed to bronchopneumonia (BP; OR: 5.0) and pulmonary tumour (PT; OR: 3.6), while the Russian Blue breed exhibited a predisposition to inflammatory lower airway diseases (OR: 3.4), BP (OR: 6.1) and interstitial lung diseases (OR: 11.1). Similarly, the Scottish Fold breed displayed predisposition to PTs (OR: 5.8). The duration of clinical signs among nasal diseases, nasopharyngeal diseases and lower tracheal/bronchial and pulmonary diseases differed significantly (p = 0.001, p = 0.012, p < 0.0001, respectively). CONCLUSIONS: The results suggest that some popular breeds in Japan are predisposed to feline respiratory diseases, especially the American Shorthair, Russian Blue and Scottish Fold breeds. The characteristics of occurrence, clinical signs and duration of each disease will aid in diagnosing, treating, preventing and elucidating the pathophysiology of feline respiratory disease.
  • Seki S, Yamaya Y, Azakami D, Fujita M
    Journal of Animal Clinical Medicine 32(4) 152-156 2023年12月  査読有り
  • Yuta Nakazawa, Takafumi Ohshima, Michio Fujita, Aki Fujiwara-Igarashi
    Veterinary medicine and science 9(2) 638-644 2022年10月17日  
    BACKGROUND: Few studies have investigated the incidence of respiratory diseases based on anatomical sites or the relationship between breed and these diseases. OBJECTIVE: The objective of this study was to investigate the prevalence of canine respiratory diseases among dogs in Japan, with relationship to the breed. METHODS: We retrospectively reviewed the medical records of dogs with respiratory symptoms and calculated the odds ratio (OR) to evaluate the relationship between breed and disease. RESULTS: A total of 1050 dogs with respiratory symptoms were included in this study. Miniature dachshunds were the most common breed affected by respiratory diseases. Among tracheobronchial diseases, there was a significant association between some small breeds and tracheobronchial collapse, miniature dachshunds (OR: 4.44, 8.43, 95% confidence interval [CI]: 3.17-6.22, 4.33-16.0) and chronic bronchitis and bronchiectasis. Among nasal diseases, miniature dachshunds (OR: 27.2, 95% CI: 16.8-44.8) and golden retrievers (OR: 21.0, 95% CI: 6.43-69.3) were the most affected by non-infectious rhinitis and nasal aspergillosis, respectively. Brachycephalic obstructive airway syndrome was the most common disease among pharyngeal and laryngeal diseases, with a relationship with breed being found in some brachycephalic breeds, and Pomeranians (OR: 2.7, 95% CI: 1.42-5.17). CONCLUSIONS: Respiratory diseases in dogs are strongly correlated with popular breeds in Japan. Miniature dachshunds, in particular, are associated with many respiratory diseases, which may differ from international reports. Thus, this result may help in the early detection, prevention, treatment, and elucidation of the pathophysiology of canine respiratory diseases.
  • Seki S, Yasuda A, Fujita M
    Journal of Animal Clinical Medicine 31(3) 106-111 2022年9月  査読有り
  • Kento Ishikawa, Tomokazu Nagashima, Yukino Machida, Akiko Yasuda, Karin Yoshida, Michio Fujita, Kazuhiko Ochiai, Daigo Azakami, Masaki Michishita
    Journal of Feline Medicine and Surgery Open Reports 8(1) 205511692210742-205511692210742 2022年1月  
    <sec><title>Case summary</title> A 4-year-old castrated male domestic shorthair cat with a continuous cough was brought to a private veterinary clinic for detailed examination. Radiography of the thoracic cavity revealed a severe radiopaque region in the caudal lobe of the right lung. At 108 days after the initial visit, CT showed a mass of 27 × 23 × 18 mm in the caudal lobe of the right lung. At that time, no abnormalities in other organs except for the lung were detected on CT and peripheral blood and blood biochemistry tests. The mass in the caudal lobe of the right lung was resected by lobectomy; it had a white surface and was firm. Histopathologically, the mass was non-encapsulated, showing an unclear boundary with surrounding tissues. The mass comprised large, round or polygonal neoplastic cells arranged in a diffuse pattern. Immunohistochemically, neoplastic cells were diffusely positive for CD20, feline leukaemia virus (FeLV) p27 and FeLV glycoprotein 70 but negative for CD3, CD204 and E-cadherin. Based on these findings, diffuse large B-cell lymphoma associated with FeLV infection was diagnosed. Although the cat showed no clinical signs of gastrointestinal or respiratory injury, a routine ultrasonography revealed thickening in the jejunum wall 196 days after lobectomy, and subsequent fine-needle aspiration examination confirmed high-grade lymphoma. </sec><sec><title>Relevance and novel information</title> This is the first report of primary pulmonary diffuse large B-cell lymphoma associated with FeLV infection in a young cat. </sec>
  • 吉田 佳倫, 久保 喜昭, 弥吉 直子, 宇佐見 彩乃, 片岡 康, 藤田 道郎
    動物臨床医学会年次大会プロシーディング 42回(2) 96-97 2021年11月  
  • Maho Nakazawa, Hirotaka Tomiyasu, Kanako Suzuki, Hajime Asada, Aki Fujiwara-Igarashi, Yuko Goto-Koshino, Aki Ohmi, Koichi Ohno, Michio Fujita, Hajime Tsujimoto
    The Journal of veterinary medical science 83(3) 456-460 2021年4月3日  
    Nasal lymphoma (NL) is the most common nasal tumor in cats, and radiotherapy, chemotherapy, or a combination of these treatments have been described as the treatment for this disease. However, the previous studies included various machines and protocols of radiotherapy. Therefore, we aimed to retrospectively compare the prognosis among cases treated with palliative hypofractionated radiotherapy, chemotherapy, and a combination of them with united machine and protocol of radiotherapy. When compared overall survival and progression free survival, there was no significant difference among these three groups. The data of this study suggested that similar efficacy could be achieved by palliative hypofractionated radiotherapy, chemotherapy, or a combination of them.
  • Masae Wada, Daisuke Hasegawa, Yuji Hamamoto, Yoshihiko Yu, Rikako Asada, Aki Fujiwara-Igarashi, Michio Fujita
    Frontiers in veterinary science 7 614026-614026 2020年  
    Meningiomas are the most common intracranial tumor in dogs and cats, and their surgical resection is often performed because they are present on the brain surface. Typical meningiomas show comparatively characteristic magnetic resonance imaging findings that lead to clinical diagnosis; however, it is necessary to capture not only macroscopic changes but also microstructural changes to devise a strategy for surgical resection and/or quality of removal. To visualize such microstructural changes, diffusion-weighted imaging (DWI) and diffusion tensor imaging (DTI) have been used in human medicine. The aim of this retrospective study was to investigate the different characteristics of the apparent diffusion coefficient (ADC) from DWI and fractional anisotropy (FA) from DTI of meningioma between dogs and cats. Statistical analyses were performed to compare ADC and FA values between the intratumoral or peritumoral regions and normal-appearing white matter (NAWM) among 13 dogs (13 lesions, but 12 each in ADC and FA analysis) and six cats (seven lesions). The NAWM of cats had a significantly lower ADC and higher FA compared to dogs. Therefore, for a comparison between dogs and cats, we used ADC and FA ratios that were calculated by dividing the subject (intra- or peritumoral) ADC and FA values by those of NAWM on the contralateral side. Regarding the intratumoral region, feline meningiomas showed a significantly lower ADC ratio and higher FA ratio than canine meningiomas. This study suggested that ADC and FA may be able to distinguish a meningioma that is solid and easy to detach, like as typical feline meningiomas.
  • 杉原 思穂, 加藤 真理子, 中村 迪香, 北原 美帆, 吉田 佳倫, 早川 典之, 小野沢 栄里, 森 昭博, 藤田 道郎
    Veterinary Nursing 24(1) 47-47 2020年1月  
  • 三浦 香奈, 関 瀬利, 安田 暁子, 吉田 佳倫, 久保 喜昭, 藤田 道郎
    日本獣医麻酔外科学雑誌 50(Suppl.2) 284-284 2019年12月  
  • Michishita M, Fujiwara-Igarashi A, Suzuki S, Hatakeyama H, Machida Y, Yoshimura H, Yamamoto M, Azakami D, Ochiai K, Ishiwata T, Fujita M
    Journal of Comparative Pathology 171 1-5 2019年8月  査読有り
  • Fujiwara-Igarashi A, Yu Y, Hamamoto Y, Hasegawa D, Fujita M
    The Journal of veterinary medical science 81(7) 1012-1016 2019年7月  査読有り
  • Ukai M, Hamamoto Y, Yu Y, Fujiwara-Igarashi A, Fujita M, Hasegawa D
    Journal of feline medicine and surgery 20(10) 962-967 2018年10月  査読有り
  • 赤松 愛, 関 瀬利, 鈴木 周二, 久保 喜昭, 澤田 治美, 長谷川 大輔, 原 康, 藤田 道郎
    日本獣医麻酔外科学雑誌 49(Suppl.1) 277-277 2018年6月  
  • Yoshihiko Yu, Daisuke Hasegawa, Yuji Hamamoto, Shunta Mizoguchi, Takayuki Kuwabara, Aki Fujiwara-Igarashi, Masaya Tsuboi, James Ken Chambers, Michio Fujita, Kazuyuki Uchida
    American journal of veterinary research 79(3) 324-332 2018年3月  査読有り
    OBJECTIVE To investigate epilepsy-related neuropathologic changes in cats of a familial spontaneous epileptic strain (ie, familial spontaneous epileptic cats [FSECs]). ANIMALS 6 FSECs, 9 age-matched unrelated healthy control cats, and 2 nonaffected (without clinical seizures)dams and 1 nonaffected sire of FSECs. PROCEDURES Immunohistochemical analyses were used to evaluate hippocampal sclerosis, amygdaloid sclerosis, mossy fiber sprouting, and granule cell pathological changes. Values were compared between FSECs and control cats. RESULTS Significantly fewer neurons without gliosis were detected in the third subregion of the cornu ammonis (CA) of the dorsal and ventral aspects of the hippocampus as well as the central nucleus of the amygdala in FSECs versus control cats. Gliosis without neuronal loss was also observed in the CA4 subregion of the ventral aspect of the hippocampus. No changes in mossy fiber sprouting and granule cell pathological changes were detected. Moreover, similar changes were observed in the dams and sire without clinical seizures, although to a lesser extent. CONCLUSIONS AND CLINICAL RELEVANCE Findings suggested that the lower numbers of neurons in the CA3 subregion of the hippocampus and the central nucleus of the amygdala were endophenotypes of familial spontaneous epilepsy in cats. In contrast to results of other veterinary medicine reports, severe epilepsy-related neuropathologic changes (eg, hippocampal sclerosis, amygdaloid sclerosis, mossy fiber sprouting, and granule cell pathological changes) were not detected in FSECs. Despite the use of a small number of cats with infrequent seizures, these findings contributed new insights on the pathophysiologic mechanisms of genetic-related epilepsy in cats.
  • Hamamoto Y, Hasegawa D, Yu Y, Asada R, Mizoguchi S, Kuwabara T, Wada M, Fujiwara-Igarashi A, Fujita M
    Frontiers in veterinary science 5 172-172 2018年  査読有り
  • Yoshihiko Yu, Daisuke Hasegawa, Aki Fujiwara-Igarashi, Yuji Hamamoto, Shunta Mizoguchi, Takayuki Kuwabara, Michio Fujita
    BMC VETERINARY RESEARCH 13(1) 389 2017年12月  査読有り
    Background: Leucine-rich glioma-inactivated (LGI) proteins play a critical role in synaptic transmission. Dysfunction of these genes and encoded proteins is associated with neurological disorders such as genetic epilepsy or autoimmune limbic encephalitis in animals and human. Familial spontaneous epileptic cats (FSECs) are the only feline strain and animal model of familial temporal lobe epilepsy. The seizure semiology of FSECs comprises recurrent limbic seizures with or without evolution into generalized epileptic seizures, while cats with antibodies against voltage-gated potassium channel complexed/ LGI1 show limbic encephalitis and recurrent limbic seizures. However, it remains unclear whether the genetics underlying FSECs are associated with LGI family genes. In the present study, we cloned and characterized the feline LGI1-4 genes and examined their association with FSECs. Conventional PCR techniques were performed for cloning and mutational analysis. Characterization was predicted using bioinformatics software. Results: The cDNAs of feline LGI1-4 contained 1674-bp, 1650-bp, 1647-bp, and 1617-bp open reading frames, respectively, and encoded proteins comprising 557, 549, 548, and 538 amino acid residues, respectively. The feline LGI1-4 putative protein sequences showed high homology with Homo sapiens, Canis familiaris, Bos taurus, Sus scrofa, and Equus caballus (92%-100%). Mutational analysis in 8 FSECs and 8 controls for LGI family genes revealed 3 non-synonymous and 14 synonymous single nucleotide polymorphisms in the coding region. Only one non-synonymous single nucleotide polymorphism in LGI4 was found in 3 out of 8 FSECs. Using three separate computational tools, this mutation was not predicted to be disease causing. No co-segregation of the disease was found with any variant. Conclusions: We cloned the cDNAs of the four feline LGI genes, analyzed the amino acid sequences, and revealed that epilepsy in FSEC is not a monogenic disorder associated with LGI genes.
  • 関 瀬利, 久保 喜昭, 弥吉 直子, 安田 暁子, 長谷川 大輔, 原 康, 藤田 道郎
    日本獣医麻酔外科学雑誌 48(Suppl.2) 208-208 2017年11月  
  • 戸田 典子, 赤羽根 僚太, 酒谷 篤, 宮川 優一, 藤田 道郎, 竹村 直行
    日本獣医麻酔外科学雑誌 48(Suppl.2) 238-238 2017年11月  
  • Masae Wada, Daisuke Hasegawa, Yuji Hamamoto, Yoshihiko Yu, Aki Fujiwara-Igarashi, Michio Fujita
    VETERINARY RADIOLOGY & ULTRASOUND 58(4) 422-432 2017年7月  査読有り
    Although MRI has become widely used in small animal practice, little is known about the validity of advanced MRI techniques such as diffusion-weighted imaging and diffusion tensor imaging. The aim of this retrospective analytical observational study was to investigate the characteristics of diffusion parameters, that is the apparent diffusion coefficient and fractional anisotropy, in dogs with a solitary intracranial meningioma or histiocytic sarcoma. Dogs were included based on the performance of diffusion MRI and histological confirmation. Statistical analyses were performed to compare apparent diffusion coefficient and fractional anisotropy for the two types of tumor in the intra- and peritumoral regions. Eleven cases with meningioma and six with histiocytic sarcoma satisfied the inclusion criteria. Significant differences in apparent diffusion coefficient value (x 10(-3) mm(2)/s) between meningioma vs. histiocytic sarcoma were recognized in intratumoral small (1.07 vs. 0.76) and large (1.04 vs. 0.77) regions of interest, in the peritumoral margin (0.93 vs. 1.08), and in the T2 high region (1.21 vs. 1.41). Significant differences in fractional anisotropy values were found in the peritumoral margin (0.29 vs. 0.24) and the T2 high region (0.24 vs. 0.17). The current study identified differences in measurements of apparent diffusion coefficient and fractional anisotropy for meningioma and histiocytic sarcoma in a small sample of dogs. In addition, we observed that all cases of intracranial histiocytic sarcoma showed leptomeningeal enhancement and/or mass formation invading into the sulci in the contrast study. Future studies are needed to determine the sensitivity of these imaging characteristics for differentiating between these tumor types.
  • Yuji Hamamoto, Daisuke Hasegawa, Shunta Mizoguchi, Yoshihiko Yu, Masae Wada, Takayuki Kuwabara, Aki Fujiwara-Igarashi, Michio Fujita
    EPILEPSY RESEARCH 133 76-82 2017年7月  査読有り
    Objective: The familial spontaneous epileptic cat (FSEC) is thought to be a good genetic model of mesial temporal lobe epilepsy. In the current study, cerebral diffusion and perfusion magnetic resonance imaging (MRI) were used to confirm the functional deficit zone in the FSEC and evaluate the effect of a single seizure on different brain regions. Methods: Six FSECs and six healthy control cats were used in this study. MRI was performed in the interictal state (resting state for control) and postictal state immediately after the vestibular stimulation-induced generalized epileptic seizure (control cats received the same stimulation as the FSECs). The apparent diffusion coefficient (ADC), fractional anisotropy and perfusion parameters (i.e., relative regional cerebral blood volume (rCBV), relative regional cerebral blood flow (rCBF), and relative regional mean transit time (rMTT)) were measured in the hippocampus, amygdala, thalamus, and gray and white matter. Results: In the interictal state, the rCBV and rMTT in the hippocampus was significantly decreased in FSECs, compared to the control. In the postictal state, FSECs had a significantly decreased ADC and an increased rCBV, rCBF, and rMTT in the hippocampus, and an increased rMTT in the amygdala, compared to the interictal state. Conclusion: This study showed that FSECs had interictal hypoperfusion in the hippocampus, and postictal hypodiffusion and hyperperfusion in the hippocampus and/or amygdala. These findings suggested that the hippocampus and/or amygdala act as the functional deficit and expanded seizure-onset zones in FSECs.
  • Shunta Mizoguchi, Daisuke Hasegawa, Yuji Hamamoto, Yoshihiko Yu, Takayuki Kuwabara, Aki Fujiwara-Igarashi, Michio Fujita
    AMERICAN JOURNAL OF VETERINARY RESEARCH 78(3) 305-310 2017年3月  査読有り
    OBJECTIVE To evaluate the usefulness of diffusion and perfusion MRI of the cerebrum in cats with familial spontaneous epilepsy (FSECs) and identify microstructural and functional deficit zones in affected cats. ANIMALS 19 FSECs and 12 healthy cats. PROCEDURES Diffusion-weighted, diffusion tensor, and perfusion-weighted MRI of the cerebrum were performed during interictal periods in FSECs. Imaging findings were compared between FSECs and control cats. Diffusion (apparent diffusion coefficient and fractional anisotropy) and perfusion (relative cerebral blood volume [rCBV], relative cerebral blood flow [rCBF], and mean transit time) variables were measured bilaterally in the hippocampus, amygdala, thalamus, parietal cortex gray matter, and subcortical white matter. Asymmetry of these variables in each region was also evaluated and compared between FSECs and control cats. RESULTS The apparent diffusion coefficient of the total amygdala of FSECs was significantly higher, compared with that of control cats. The fractional anisotropy of the right side and total hippocampus of FSECs was significantly lower, compared with that of control cats. The left and right sides and total hippocampal rCBV and rCBF were significantly lower in FSECs than in control cats. The rCBV and rCBF of the parietal cortex gray matter in FSECs were significantly lower than in control cats. CONCLUSIONS AND CLINICAL RELEVANCE In FSECs, diffusion and perfusion MRI detected microstructural changes and hypoperfusion (lowered function) in the cerebrum during interictal periods from that of healthy cats. These findings indicated that diffusion and perfusion MRI may be useful for noninvasive evaluation of epileptogenic foci in cats.
  • Aki Fujiwara-Igarashi, Koichi Shimizu, Masaki Michishita, Yoshihiko Yu, Yuji Hamamoto, Daisuke Hasegawa, Michio Fujita
    Journal of Veterinary Medical Science 79(12) 1916-1919 2017年  査読有り
    A 16-year-old castrated male mongrel cat presented with swelling under the left pinna and a 3 -month history of voice change. Laryngeal endoscopy revealed circumferential oedema around the arytenoid cartilages and hypersecretion of saliva. Histopathological examination of the mass around the left ear canal was considered the primary lesion that originated from cutaneous apocrine adenocarcinoma or parotid gland adenocarcinoma, and it metastasized to the larynx, lung and medial retropharyngeal lymph nodes. This report provides new insights into feline laryngeal diseases which could result in laryngeal metastasis with slight mucosal irregularity alone and without obvious radiographic abnormalities. Therefore, histopathological examination should be performed when a cat presents clinical signs such as stridor, dysphonia or voice change without any mass-forming laryngeal lesion.
  • Yuji Hamamoto, Daisuke Hasegawa, Shunta Mizoguchi, Yoshihiko Yu, Masae Wada, Takayuki Kuwabara, Aki Fujiwara-Igarashi, Michio Fujita
    BMC VETERINARY RESEARCH 12(1) 248 2016年11月  査読有り
    Background: Epilepsy is the most common neurological disease in veterinary practice. However, contrary to human medicine, epilepsy classification in veterinary medicine had not been clearly defined until recently. A number of reports on canine epilepsy have been published, reflecting in part updated proposals from the human epilepsy organization, the International League Against Epilepsy. In 2015, the International Veterinary Epilepsy Task Force (IVETF) published a consensus report on the classification and definition of canine epilepsy. The purpose of this retrospective study was to investigate the etiological distribution, survival time of dogs with idiopathic epilepsy (IdE) and structural epilepsy (StE), and risk factors for survival time, according to the recently published IVETF classification. We investigated canine cases with epilepsy that were referred to our teaching hospital in Japan during the past 10 years, and which encompassed a different breed population from Western countries. Results: A total of 358 dogs with epilepsy satisfied our etiological study criteria. Of these, 172 dogs (48 %) were classified as IdE and 76 dogs (21 %) as StE. Of these dogs, 100 dogs (consisting of 65 with IdE and 35 with StE) were included in our survival study. Median survival time from the initial epileptic seizure in dogs with IdE and StE was 10.4 and 4.5 years, respectively. Median lifespan of dogs with IdE and StE was 13.5 and 10.9 years, respectively. Multivariable analysis demonstrated that risk factors for survival time in IdE were high seizure frequency (&gt;= 0.3 seizures/month) and focal epileptic seizures. Conclusions: Focal epileptic seizures were identified as a risk factor for survival time in IdE. Clinicians should carefully differentiate seizure type as it is difficult to identify focal epileptic seizures. With good seizure control, dogs with IdE can survive for nearly the same lifespan as the general dog population. Our results using the IVETF classification are similar to previous studies, although some features were noted in our Japanese canine population (which was composed of mainly small-breed dogs), including a longer lifespan in dogs with epilepsy and a larger percentage of meningoencephalomyelitis of unknown origin in dogs with StE.
  • Wada M, Hasegawa D, Hamamoto Y, Asai A, Shouji A, Chambers J, Uchida K, Fujita M
    The Journal of veterinary medical science 78(1) 101-104 2016年1月  査読有り
    A 12-year-old spayed female Labrador retriever was presented with forebrain signs. Brain MRI revealed a huge cystic lesion with the thickened falx in the frontal region. The brain parenchyma surrounding the lesion showed significant signs of a mass effect and also increased intracranial pressure. However, the dog suddenly became lucid after about two weeks, and an MRI scan one month after the initial study revealed a dramatically shrunken cystic lesion. The dog survived for over a year until it was euthanized for other reasons, and the brain lesion was diagnosed as a cystic meningioma histologically. To the authors' knowledge, this is the first report that described the reduction of the cystic lesion of a cystic meningioma in dogs.
  • Fujiwara-Igarashi A, Igarashi H, Hasegawa D, Fujita M
    Journal of veterinary internal medicine / American College of Veterinary Internal Medicine 29(6) 1643-1647 2015年11月  査読有り
  • Mizoguchi S, Hasegawa D, Kuwabara T, Hamamoto Y, Ogawa F, Fujiwara A, Matsuki N, Fujita M
    Epilepsy research 108(10) 1940-1944 2014年12月  査読有り
  • Fujiwara-Igarashi A, Fujimori T, Oka M, Nishimura Y, Hamamoto Y, Kazato Y, Sawada H, Yayoshi N, Hasegawa D, Fujita M
    Veterinary journal (London, England : 1997) 202(3) 455-461 2014年12月  査読有り
  • Hasegawa D, Mizoguchi S, Kuwabara T, Hamamoto Y, Ogawa F, Matsuki N, Uchida K, Fujita M
    Epilepsy research 108(6) 1018-1025 2014年8月  査読有り
  • Tamura M, Hasegawa D, Uchida K, Kuwabara T, Mizoguchi S, Ochi N, Fujita M
    Journal of feline medicine and surgery 15(12) 1137-1140 2013年12月  査読有り
  • 澤田 治美, 安田 暁子, 弥吉 直子, 藤田 道郎
    獣医麻酔外科学雑誌 44(Suppl.1) 262-262 2013年6月  
  • Fujiwara A, Kobayashi T, Kazato Y, Yayoshi N, Fujita M
    The Journal of small animal practice 54(2) 80-86 2013年2月  査読有り
  • Hasegawa D, Tamura S, Nakamoto Y, Matsuki N, Takahashi K, Fujita M, Uchida K, Yamato O
    PloS one 8(12) e83455-e83455 2013年  査読有り
  • Hasegawa D, Uchida K, Kuwabara T, Mizoguchi S, Yayoshi N, Fujita M
    The Journal of veterinary medical science 74(11) 1517-1521 2012年11月  査読有り
  • 桑原 孝幸, 長谷川 大輔, 溝口 俊太, 武藤 陽信, 藤田 道郎
    日本獣医画像診断学会抄録 50回 91-91 2012年1月  
  • 長谷川 大輔, 桑原 孝幸, 溝口 俊太, 土持 渉, 池田 冬美, 水野 壮司, 宮崎 美穂, 内田 恵子, 神田 幹雄, 藤田 道郎
    日本獣医画像診断学会抄録 50回 92-92 2012年1月  
  • 溝口 俊太, 長谷川 大輔, 桑原 孝幸, 藤田 道郎
    日本獣医画像診断学会抄録 50回 93-93 2012年1月  
  • Daisuke Hasegawa, Osamu Yamato, Yuya Nakamoto, Tsuyoshi Ozawa, Akira Yabuki, Kazuhito Itamoto, Takayuki Kuwabara, Michio Fujita, Kimimasa Takahashi, Shunta Mizoguchi, Hiromitsu Orima
    SCIENTIFIC WORLD JOURNAL 2012年  査読有り
    GM1 gangliosidosis is a fatal neurodegenerative lysosomal storage disease caused by an autosomal recessively inherited deficiency of beta-galactosidase activity. Effective therapies need to be developed to treat the disease. In Shiba Inu dogs, one of the canine GM1 gangliosidosis models, neurological signs of the disease, including ataxia, start at approximately 5 months of age and progress until the terminal stage at 12 to 15 months of age. In the present study, serial MR images were taken of an affected dog from a model colony of GM1 gangliosidosis and 4 sporadic clinical cases demonstrating the same mutation in order to characterize the MRI features of this canine GM1 gangliosidosis. By 2 months of age at the latest and persisting until the terminal stage of the disease, the MR findings consistently displayed diffuse hyperintensity in the white matter of the entire cerebrum on T2-weighted images. In addition, brain atrophy manifested at 9 months of age and progressed thereafter. Although a definitive diagnosis depends on biochemical and genetic analyses, these MR characteristics could serve as a diagnostic marker in suspect animals with or without neurological signs. Furthermore, serial changes in MR images could be used as a biomarker to noninvasively monitor the efficacy of newly developed therapeutic strategies.
  • Hasegawa D, Yamato O, Nakamoto Y, Ozawa T, Yabuki A, Itamoto K, Kuwabara T, Fujita M, Takahashi K, Mizoguchi S, Orima H
    The Scientific World Journal 2012(250197) 250197-10 2012年  査読有り
  • 藤田 道郎
    日本獣医がん学会雑誌 3(2) 13-19 2012年  査読有り
    犬の四肢軟部組織肉腫14症例に対して術後低分割放射線治療を行い、治療成績や放射線障害について回顧的研究を行った。再発率は7.1%、放射線障害は85.7%で何らかの障害が認められたが、現在まで重篤な障害は認められず、再発に対する影響もなかった。一、二および三年生存率はそれぞれ100、92.9および66.7%あった。また一、二および三年腫瘍コントロール率はそれぞれ85.7、71.4、57.1%であった。以上のことから、犬の四肢の軟部組織肉腫に対する術後低分割放射線治療は従来の根治目的の放射線治療スケジュールと比較して治療効果に大きな差はなく、麻酔のリスクや動物への負担、オーナーへの負担を軽減する上で有用な照射方法の1つと考えられた。
  • Kuwabara T, Hasegawa D, Ogawa F, Kobayashi M, Fujita M, Suzuki H, Matsuki N, Orima H
    Epilepsy research 92(1) 85-88 2010年11月  査読有り
  • Kuwabara T, Hasegawa D, Kobayashi M, Fujita M, Orima H
    Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association 51(5) 485-490 2010年9月  査読有り
  • 藤田 道郎, 島倉 秀勝, 長谷川 大輔, 谷口 明子, 河上 栄一, 織間 博光
    日本獣医がん学会雑誌 1(1) 20-25 2010年1月  
  • Masanori Kobayashi, Akinori Nakamura, Daisuke Hasegawa, Michio Fujita, Hiromitsu Orima, Shin'ichi Takeda
    MUSCLE & NERVE 40(5) 815-826 2009年11月  査読有り
    Duchenne muscular dystrophy (DMID) is a devastating muscle disorder that is characterized by progressive muscle necrosis, fibrosis, and fatty infiltration. To examine the temporospatial pathological changes, a noninvasive evaluation method such as magnetic resonance imaging (MRI) is needed. The aim of this study was to precisely assess muscle necrosis and inflammation based on a sequence of T2-weighted imaging (T2WI), gadolinium-enhanced imaging, and selective fat suppression, chemical shift selective T2-weighted imaging (CHESS-T2WI), on a 3.0-Tesla MRI unit in 3-month-old and 7-year-old dogs with canine X-linked muscular dystrophy (CXMDJ), a suitable animal model for DMD. The results show that CHESS-T2WI was more sensitive and useful from the early to late stages of CXMDJ than T2WI or contrast enhancement imaging in the evaluation of muscle necrosis, because these latter sequences can be influenced by fatty infiltration or interstitial connective tissues. Muscle Nerve 40: 815-826, 2009
  • 長谷川 大輔, 桑原 孝幸, 小林 正典, 藤田 道郎, 鈴木 浩悦, 鈴木 勝士, 織間 博光
    てんかん研究 27(2) 323-323 2009年9月  
  • Hasegawa D, Uchida K, Kobayashi M, Kuwabara T, Ide T, Ogawa F, Fujita M, Orima H
    Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association 50(3) 298-300 2009年5月  査読有り
  • Yumi Takaishi, Yukari Yoshida, Kazuhide Nakagaki, Michio Fujita, Akiko Taniguchi, Hiromitsu Orima
    Journal of Veterinary Medical Science 70(12) 1333-1335 2008年12月  査読有り
    SART-1, a squamous cell carcinoma (SCC) antigen recognized by cytotoxic T lymphocytes, has been useful in human cancer therapy. The SART-1259 peptide is a potential candidate for vaccine. The present study examined an orthologue of the mRNA coding this peptide in canine SCCs. Specimens were obtained from seven canine patients with SCC, and the mRNA was isolated from the samples. The SART-1 and beta-actin genes were amplified by reverse-transcription polymerase chain reaction, using the isolated mRNA as a template. Canine SART-1 was amplified in six of the seven specimens, while beta-actin was detected in all the samples. In dogs, carcinomas expressing SART-1 could be a target for cytotoxic T lymphocyte mediated immunotherapy.
  • Hasegawa D, Kobayashi M, Kuwabara T, Ohmura T, Fujita M, Orima H
    Journal of feline medicine and surgery 10(4) 418-421 2008年8月  査読有り
  • Hasegawa D, Kobayashi M, Fujita M, Uchida K, Orima H
    The Journal of veterinary medical science / the Japanese Society of Veterinary Science 70(6) 615-617 2008年6月  査読有り

MISC

 96

書籍等出版物

 19

担当経験のある科目(授業)

 3

共同研究・競争的資金等の研究課題

 5