基本情報
- 所属
- 日本獣医生命科学大学 獣医学部 獣医学科 准教授
- 学位
- 博士(農学)(1997年6月 北海道大学)
- J-GLOBAL ID
- 200901098567219915
- researchmap会員ID
- 6000000826
研究分野
1経歴
5-
2005年 - 現在
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2001年 - 2005年
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2000年 - 2001年
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1994年4月 - 1997年3月
学歴
3-
1993年4月 - 1997年6月
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1991年4月 - 1993年3月
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1987年4月 - 1991年3月
論文
29-
Chemosphere 321 138032-138032 2023年4月 査読有り
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Biotechnology progress e3287 2022年7月11日 査読有りTo clarify the relationship between irreversible inactivation and intracellular protein denaturation of Saccharomyces pastorianus by low-pressure carbon dioxide microbubbles (CO2 MB) treatment, a storage test of S. pastorianus cells treated with CO2 MB was performed, and the effect on the intracellular protein was investigated. In the storage test, the S. pastorianus population, which decreased below the detection limit by CO2 MB treatment at a temperature of 45 and 50°C (MB45 and MB50), and thermal treatment at a temperature of 80°C (T80), remained undetectable during storage for 3 weeks at 25°C. However, 4.1 and 1.3-logs of the S. pastorianus populations, which survived after CO2 MB treatment at temperatures of 35 and 40°C (MB35 and MB40), increased gradually during storage for 3 weeks at 25°C. Insolubilization of intracellular proteins in S. pastorianus increased with increasing the temperature of CO2 MB treatment. Activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) identified as one of the insolubilized proteins increased at MB35 and MB40 than non-treatment but disappeared at MB45 and MB50, and T80. Therefore, it was revealed that S. pastorianus cells inactivated below the detection level by CO2 MB treatment did not regrow and that the denaturation of intracellular proteins of S. pastorianus was caused by CO2 MB and thermal treatments. Furthermore, it was suggested that denaturation of intracellular vital enzymes was an important factor for achieving irreversible inactivation of S. pastorianus by CO2 MB and thermal treatments.
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The Journal of Veterinary Medical Science 81(9) 1238-1248 2019年7月 査読有り
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International journal of systematic and evolutionary microbiology 68(8) 2437-2442 2018年8月 査読有りAmong non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of the largest groups, consisting of 18 species of slow-growing mycobacteria. In 2009, a case of NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on the chest, back and extremities, and an M. simiae-like organism was isolated from skin biopsy specimens obtained from one of these lesions. Based on several assessments, including multiple-gene analyses, biochemical characterization and drug susceptibility testing, we concluded that this isolate represented a novel species of NTM, and proposed the name 'Mycobacterium shigaense'. Since 2009, five more cases of NTM-associated infectious disease in which there was a suspected involvement of 'M. shigaense' have been reported. Interestingly, four of these six cases occurred in Shiga Prefecture. Here we performed multiple-gene phylogenetic analyses, physiological and biochemical characterization tests, drug susceptibility tests, and profiling of proteins, fatty acids and mycolic acids of eight clinical isolates from the six suspected 'M. shigaense' cases. The results confirmed that all of the clinical isolates were 'M. shigaense', a slow-growing, scotochromogenic species. Here M. shigaense is validly proposed as a new member of the M. simiae complex, with the type strain being UN-152T (=JCM 32072T=DSM 46748T).
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CANCER SCIENCE 109 266 2018年1月 査読有り
MISC
49講演・口頭発表等
14担当経験のある科目(授業)
5-
2020年4月 - 現在生命科学研究論(分担) (日本獣医生命科学大学)
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研究用機器論(分担) (日本獣医生命科学大学)
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化学実習 (日本獣医生命科学大学)
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化学 I (日本獣医生命科学大学)
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生体分子化学 (日本獣医生命科学大学)
共同研究・競争的資金等の研究課題
3-
日本学術振興会 科学研究費助成事業 基盤研究(C) 2020年4月 - 2023年3月
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文部科学省 科学研究費補助金(挑戦的萌芽研究) 2015年 - 2016年
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文部科学省 科学研究費補助金(萌芽研究) 2008年 - 2008年