研究者業績

落合 和彦

オチアイ カズヒコ  (OCHIAI KAZUHIKO)

基本情報

所属
日本獣医生命科学大学 獣医学部 獣医学科 獣医衛生学研究室 准教授 (DVM. Ph D.)
学位
博士(獣医学)(2005年3月 岐阜大学)

J-GLOBAL ID
201001023189960831
researchmap会員ID
6000021447

外部リンク

研究キーワード

 3

論文

 102
  • T Oonuma, M Morimatsu, K Ochiai, B Syuto
    JOURNAL OF VETERINARY MEDICAL SCIENCE 65(10) 1123-1126 2003年10月  査読有り
    Mammary tumors are common in cats. As mutations in human Brca2 confer an increased risk of breast cancer, the full-length cDNA of the feline homologue of Brca2 was sequenced to obtain a basis for studying the relationship between its function and susceptibility to mammary tumors. The feline Brca2 cDNA is 10 kb long, and encodes 3,371 amino acids. The amino acid sequence of feline Brca2 shares low homology with the Brca2 of other mammals, e.g., 53% homology with the murine protein. Analysis of the expression pattern of the feline Brca2 gene revealed that, as previously reported for other mammals, it is transcribed in various tissues, including the mammary gland.
  • K Ochiai, M Morimatsu, N Tomizawa, B Syuto
    JOURNAL OF VETERINARY MEDICAL SCIENCE 63(10) 1103-1108 2001年10月  査読有り筆頭著者
    Mammary tumors are the most common neoplasm in female dogs. Canis canis, and in women. Mutations in human Brca2 confer an increased risk of female breast cancer. Previous studies have shown that the Brca2 tumor suppressor protein interacts with the recombinational repair protein Rad51. We cloned the full-length cDNA of the canine homologues of Brca2 and Rad51 to obtain a basis for studying their relationship with susceptibility to mammary tumors. The canine Brca2 and Rad51 cDNAs are 11 and 1.5 kb long, encoding 3,471 and 339 amino acids, respectively. The amino acid sequence of canine Brca2 showed 68% homology with the human protein., and 58% homology with a murine protein. There were highly conserved regions in the C-terminus of all three proteins, where the Rad51 interacting domain and putative nuclear localization signals are located. Comparing with the partial genomic sequence previously reported, we found possible nuclear polymorphisms in exon 11, some of which result in amino acid substitutions. On the other hand, canine Rad51 protein had extremely high homology (99%) to the human and murine proteins. Expression of both Brca2 and Rad51 was detected in the mammary gland, suggesting that these two genes interact in the canine mammary gland.

MISC

 33

講演・口頭発表等

 81

担当経験のある科目(授業)

 6

共同研究・競争的資金等の研究課題

 13

産業財産権

 2