獣医学科

Yoshitsugu Ochiai

  (落合 由嗣)

Profile Information

Affiliation
Professor, Faculty of Veterinary Science, School of Veterinary Medicine, Nippon Veterinary and Life Science University
Degree
Doctor(Veterinary medicine)(Gifu University)

J-GLOBAL ID
200901022994569599
researchmap Member ID
5000017398

Research History

 1

Papers

 31
  • Yoshitsugu Ochiai, Yuko Yoshikawa, Takashi Takano, Makoto Mori, Ryo Hondo, Mariko Mochizuki, Fukiko Ueda
    The Journal of veterinary medical science, 82(6) 735-739, Apr 9, 2020  Peer-reviewedLead author
    A Japanese resident bird, Phalacrocorax carbo hanedae (Japanese name: Kawa-u), was threatened with extinction due to deterioration of its habitat in the 1970s, but the population has since recovered thanks to environmental protection measures. This study analyzed the genetic diversity of 18 Kawa-u individuals living in the basins of the Abe and Warashina rivers in Shizuoka Prefecture, Japan. We obtained seven haplotypes of mitochondrial D-loop sequences and compared them with 49 European P. carbo D-loop haplotypes. We identified four new haplotypes but no clear genetic evidence distinguishing the Kawa-u as a distinct subspecies of P. carbo. Our results suggest the need for further surveillance of the P. carbo genetic lineage, regardless of the geographical distribution.
  • Yuko Yoshikawa, Kei Sugimoto, Yoshitsugu Ochiai, Norio Ohashi
    Microbiology and immunology, 64(4) 270-279, Apr, 2020  Peer-reviewed
    Anaplasma phagocytophilum, an obligate intracellular bacterium that propagates within host granulocytes, is considered to modify the host intracellular environment for pathogenesis. However, the mechanism(s) underlying such host modifications remain unclear. Here, we aimed to investigate the relation between A. phagocytophilum and endoplasmic reticulum (ER) stress in THP-1 cells. A. phagocytophilum activated the three ER stress sensors: inositol-requiring enzyme-1 (IRE1), protein kinase RNA-like endoplasmic reticulum kinase (PERK), and activating transcription factor-6 (ATF6). IRE1 activation occurred immediately after host cell invasion by A. phagocytophilum; however, the activated IRE1-induced splicing of X-box-binding protein 1 was not promoted during A. phagocytophilum infection. This suppression was sustained even after the doxycycline-mediated elimination of intracellular A. phagocytophilum. IRE1 knockdown accelerated A. phagocytophilum-induced apoptosis and decreased intracellular A. phagocytophilum. These data suggest that A. phagocytophilum utilizes IRE1 activation to promote its own intracellular proliferation. Moreover, PERK and ATF6 partially mediated A. phagocytophilum-induced apoptosis by promoting the expression of CCAAT/enhancer-binding protein homologous protein, which induces the transcription of several proapoptotic genes. Thus, A. phagocytophilum possibly manipulates the host ER stress signals to facilitate intracellular proliferation and infection of surrounding cells before/after host cell apoptosis.
  • Yoshitsugu Ochiai, Yuko Yoshikawa, Mariko Mochizuki, Takashi Takano, Fukiko Ueda
    Food microbiology, 86 103312-103312, Apr, 2020  Peer-reviewedLead authorCorresponding author
    Some Listeria monocytogenes strains are persistent in food processing environments, where this pathogen may be subjected to various stresses. This study aimed to elucidate the response of persistent strains of L. monocytogenes to low pH and H2O2 exposure. Almost all of the persistent strains examined were highly susceptible to low pH, whereas H2O2 susceptibility was comparable to that of control strains. Two persistent strains isolated from the same sample, however, exhibited lower susceptibility to low pH. These findings suggest an acid-susceptible phenotype predominates in the habitat, indicating that environmental conditions contribute to the establishment of persistence. Representative strains exhibiting acid-susceptible and less acid-susceptible phenotypes were further investigated regarding acid response characteristics. Less acid-susceptible strains exhibited increased survival in acidified brain heart infusion (BHI) broth compared with acidified phosphate-buffered saline (PBS). These strains also exhibited increased survival in acidified PBS containing glucose and glutamate, which are involved in acid response mechanisms, compared with acidified PBS alone. However, neither acidified BHI broth nor exogenous glucose and glutamate increased survival of acid-susceptible strains. An adaptive acid tolerance response of the acid-susceptible phenotype was observed, but this was limited compared with that of the less acid-susceptible phenotype.
  • Yuko Yoshikawa, Yoshitsugu Ochiai, Mariko Mochizuki, Takashi Takano, Ryo Hondo, Fukiko Ueda
    Japanese journal of infectious diseases, 71(5) 373-377, Sep 21, 2018  Peer-reviewed
    The level of Listeria monocytogenes contamination of domestic retail meat in Tokyo, Japan, was assessed by comparison of isolates from 2004 to 2007 with those isolated before 2003. The overall prevalence of L. monocytogenes among these samples significantly diminished over time (1998-2003, 28.0%; 2004-2007, 17.6%) reflecting a significant decrease in the frequency of contamination of beef. Serotype 1/2a was isolated most frequently, reflecting a change in the predominant serotype in pork from 1/2c to 1/2a. We performed a simple genetic subtyping method based on 3 genes, iap, sigB, and actA, as well as traditional multilocus sequence typing to classify the allele types (ATs). No extensive variation among sequence types was detected. However, increased genetic diversity among the ATs of the 3 genes in the 2004-2007 isolates was evident. We identified AT 26 of the iap gene, which was not previously reported in Japanese isolates, and 6 ATs of the sigB gene, including 4 with nonsense mutations not currently registered in L. monocytogenes DNA databases. sigB is an evolutionally conserved gene that plays a role in the stress response. Our results indicate that the sigB gene may be relatively unstable among L. monocytogenes strains circulating in Japan.
  • Yoshitsugu Ochiai, Fumiya Yamada, Yuko Yoshikawa, Mariko Mochizuki, Takashi Takano, Ryo Hondo, Fukiko Ueda
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 259 52-58, Oct, 2017  Peer-reviewedLead authorCorresponding author
    The food-borne pathogen Listeria monocytogenes is present persistently in food processing environments, where this bacterium is exposed to various stress factors, including oxidative stress. This study aimed to elucidate the temperature-dependent response of L. monocytogenes to H2O2 exposure and the phenotypic changes in colony formation by H2O2-treated bacteria. Survival curves indicated an increase in the resistance to H2O2 in L. monocytogenes as the temperature decreased during the stress exposure procedure. Transcriptional induction of genes with key roles in response to H2O2, including sigB and kat, was observed at 37 degrees C, but not at 20 degrees C, whereas other stress response genes were induced at both temperatures. Following H2O2 exposure, L. monocytogenes produced small colony phenotypes and the colony size decreased in a stress exposure duration-dependent manner. Resuscitated cells with no ability to form colonies in the absence of sodium pyruvate were also found. Our findings show the possibility that a sequential transition in the injury phenotype from small colony phenotype to resuscitated cells occurred during the course of exposure to H2O2. The higher H2O2 resistance at 20 degrees C than 37 degrees C suggests further investigation of the response to H2O2 exposure under the lower temperatures, including refrigeration temperature, which may contribute to elucidation of bacterial survival over extended time periods in food-processing environments.

Misc.

 4
  • Mariko Mochizuki, Mayumi Shiozawa, Makoto Mori, Hiroshi Kajigaya, Shin ichi Hayama, Yoshitsugu Ochiai, Ryo Hondo, Fukiko Ueda
    Energy, Environment and Economics Research Compendium, 197-205, Feb 1, 2013  
    © 2013 by Nova Science Publishers, Inc. All rights reserved. The cadmium (Cd) content in the kidneys and livers of wild birds was compared after classification based on the type of migration and the breeding area. A high Cd content was detected in the organs of Pacific loons breeding in North America, but these birds were thought to have been affected by oil contamination. The Cd content of organs from spotbill ducks, which are birds that are resident in Japan, tended to be higher than those of other birds that breed outside Japan. The rank order of the mean Cd level with respect to location was "only in Eurasia" < "both Eurasia and North America" < "only in North America". The type of migration did not affect the Cd content of the organs. We also investigated the relationship between the Cd content of the organs and the Japanese environmental concentration of Cd. The Cd contents of organs from birds collected on the shore of the Japan Sea were generally higher than those of the birds collected near the Pacific Ocean. These results seem to be related to the concentration of Cd in the soil. From these results, we showed that the differences in the Cd content in organs from Japanese wild birds were related to their breeding area but not to the type of migration. Further, the area from which the wild birds were collected was strongly related to the Cd content of the organs. This demonstrated the relationship between the environmental and soil conditions in Japan and the level of pollution of the birds studied.
  • Mariko Mochizuki, Mayumi Shiozawa, Makoto Mori, Hiroshi Kajigaya, Shin Ichi Hayama, Yoshitsugu Ochiai, Ryo Hondo, Fukiko Ueda
    Advances in Environmental Research. Volume 4, 269-278, Jan 1, 2010  
    © 2010 by Nova Science Publishers, Inc. All rights reserved. The cadmium (Cd) content in the kidneys and livers of wild birds was compared after classification based on the type of migration and the breeding area. A high Cd content was detected in the organs of Pacific loons breeding in North America, but these birds were thought to have been affected by oil contamination. The Cd content of organs from spotbill ducks, which are birds that are resident in Japan, tended to be higher than those of other birds that breed outside Japan. The rank order of the mean Cd level with respect to location was "only in Eurasia" < "both Eurasia and North America" < "only in North America". The type of migration did not affect the Cd content of the organs. We also investigated the relationship between the Cd content of the organs and the Japanese environmental concentration of Cd. The Cd contents of organs from birds collected on the shore of the Japan Sea were generally higher than those of the birds collected near the Pacific Ocean. These results seem to be related to the concentration of Cd in the soil. From these results, we showed that the differences in the Cd content in organs from Japanese wild birds were related to their breeding area but not to the type of migration. Further, the area from which the wild birds were collected was strongly related to the Cd content of the organs. This demonstrated the relationship between the environmental and soil conditions in Japan and the level of pollution of the birds studied.
  • 保坂 三継, 落合 由嗣, 矢野 一好
    用水と廃水, Vol. 44 pp. 295-303(4) 295-303, 2002  
  • 保坂 三継, 落合 由嗣, 矢野 一好
    東京都立衛生研究所研究年報, Vol. 52 pp. 254-259(52) 254-259, 2001  

Presentations

 2

Teaching Experience

 7

Research Projects

 5