研究者業績

山本 一郎

ヤマモト イチロウ  (Ichiro Yamamoto)

基本情報

所属
日本獣医生命科学大学 獣医学部 獣医学科 教授
学位
博士(農学)(名古屋大学)

J-GLOBAL ID
200901017318751437
researchmap会員ID
5000041338

主にネコの肥満について研究しております。専門は遺伝子工学、内分泌学と発生生物学です。

論文

 93
  • Kentaro Katayama, Junya Ito, Rei Murakami, Ayako Yamashita, Hotaka Sasajima, Satomi Narahashi, Junko Chiba, Ichiro Yamamoto, Wataru Fujii, Yuki Tochigi, Hiroetsu Suzuki
    Mammalian Genome 2024年4月24日  査読有り
  • Ichiro Yamamoto, Masaki Michishita, Koki Fujita, Tamami Sakai, Noriyasu Sasaki, Koh Kawasumi
    General and comparative endocrinology 353 114520-114520 2024年4月18日  査読有り筆頭著者責任著者
    G protein-coupled receptor 84 (GPR84) was cloned as an orphan receptor, and medium-chain fatty acids were then revealed as endogenous ligands. GPR84 is expressed in immune cells and is believed to protect liver function from lipotoxicity caused by overeating and high-fat diet intake. This study aimed to present the molecular characterization of GPR84 in domestic cats. The deduced amino acid sequence of the feline GPR84 shows high sequence homology (83-89 %) with the orthologues from other mammalians by cDNA cloning of feline GPR84. Remarkably high mRNA expression was observed in the bone marrow by Q-PCR analysis. The inhibition of intracellular cAMP concentration was observed in cells transfected with feline GPR84 and treated with medium-chain fatty acids. Immunostaining of GPR84 and free fatty acid receptor 2 (FFAR2)/GPR43 in the bone marrow, where high mRNA expression was observed, showed reactions in macrophages and myeloid cells. To clarify whether the receptor formed homo/hetero-merization, GPR84 and FFARs were analyzed using Nano-Luc binary technology and NanoLuc bioluminescence resonance energy transfer technologies, which revealed that GPR84 formed more heteromers with FFAR2 than homomers with each other. In addition, when GPR84 and FFAR2/GPR43 were cotransfected in the cell, their localization on the cell membrane was reduced compared with that when single receptors were transfected. These results indicated that GPR84 is a functional receptor protein that is expressed in cat tissues and may have a protein-protein interaction with FFAR2/GPR43 on the cell membrane.
  • Ichiro Yamamoto, Koh Kawasumi, Kozo Ohkusu‐Tsukada, Toshiro Arai
    Veterinary Medicine and Science 7(1) 77-85 2021年1月  査読有り筆頭著者責任著者
    G protein-coupled receptors 41 and 43 were identified and characterized as free fatty acid receptors (FFAR) 3 and 2, respectively. FFAR2 and FFAR3 mediate short-chain fatty acids (SCFAs) as signalling molecules. The present study aimed to give molecular characterization of FFAR2 and FFAR3 in the domestic cat. High homology with that in other mammals was revealed by cDNA cloning of cat FFAR2 FFAR3. We analyzed the tissue distribution of cat FFAR2 and FFAR3 mRNA using quantitative polymerase chain reaction. The inhibition of intracellular cAMP concentrations was observed in cells transfected with cat FFAR2 or FFAR3 and treated with SCFAs. The activation of nuclear factor of activated T cells-luciferase reporter was only observed in cat FFAR2 transfected cells but not in FFAR3. Split luciferase assay (NanoLuc Binary Technology; NanoBiT) for FFAR2 or FFAR3 and Arrestin-3/β-arrestin-2 revealed acetate-/propionate-induced recruitment to cat FFAR2 or FFAR3 in CHO-K1 cells. Our results indicate that FFAR2 and FFAR3 are functional receptor proteins that are expressed in cat tissues and show differential distribution patterns.
  • Takayuki Mizorogi, Motoo Kobayashi, Kenji Ohara, Yuki Okada, Ichiro Yamamoto, Toshiro Arai, Koh Kawasumi
    Veterinary Medicine: Research and Reports Volume 11 131-137 2020年11月  査読有り
  • Koh Kawasumi, Tae Murai, Takayuki Mizorogi, Yuki Okada, Ichiro Yamamoto, Kohei Suruga, Kazunari Kadokura, Toshiro Arai
    Frontiers in Nutrition 5 2018年9月7日  査読有り

MISC

 16

講演・口頭発表等

 78

担当経験のある科目(授業)

 10

所属学協会

 8

共同研究・競争的資金等の研究課題

 11