基本情報
- 所属
- 日本獣医生命科学大学 獣医内科学研究室 講師
- 学位
- 博士(獣医学)(日本獣医生命科学大学)
- 研究者番号
- 80825216
- ORCID ID
- https://orcid.org/0000-0001-9451-5854
- J-GLOBAL ID
- 201801016742323716
- researchmap会員ID
- B000290975
- 外部リンク
研究キーワード
31経歴
11-
2024年4月 - 現在
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2022年7月 - 現在
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2022年4月 - 現在
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2022年4月 - 現在
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2020年10月 - 現在
学歴
2-
2010年4月 - 2014年3月
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2004年4月 - 2010年3月
委員歴
3-
2021年7月 - 現在
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2021年7月 - 現在
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2021年7月 - 2023年8月
受賞
10-
2021年3月
論文
56-
Frontiers in Veterinary Science 11 2024年8月8日 査読有りIntroduction The therapeutic efficacy of mesenchymal stromal cells (MSCs) in inflammatory bowel disease is not completely known and is not consistent. Priming with inflammatory cytokines has been proposed to adapt MSCs to an inflammatory environment to have them ready to counteract it, but may have undesirable effects on MSCs, such as increased immunogenicity. In this study, we hypothesized that priming MSCs with inflamed intestinal tissue would more effectively enhance their therapeutic effect on intestinal inflammation. Methods The capacity of canine adipose-derived MSCs (cADSCs) primed with colon tissue homogenates from mice with experimentally induced colitis or a combination of tumor necrosis factor-α and interferon-γ to inhibit T-cell proliferation was analyzed, along with their own apoptosis, proliferation, cell surface marker expression, and transcriptome. In addition, colitis mice were treated with the primed cADSCs to assess colitis severity and immune cell profile. Results Priming with cytokines induced apoptosis, decreased cell proliferation, and major histocompatibility complex-II gene expression in cADSCs, but these adverse effects were mild or absent with colitis-tissue priming. cADSCs primed with colitis tissue reduced the severity of colitis via the induction of M2 macrophages and T-regulatory cells and suppression of T-helper (Th)1/Th17-cell responses, and their effects were comparable to those of cytokine-primed cells. Discussion Our results emphasize the importance of the activation of MSCs by the appropriate microenvironment to maximize their therapeutic effect.
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Biology 2024年4月30日 査読有り筆頭著者責任著者
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Journal of Veterinary Internal Medicine 2024年4月25日 査読有り筆頭著者責任著者
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International Journal of Molecular Sciences 2023年12月14日 査読有り
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Journal of veterinary internal medicine 2023年8月18日 査読有り責任著者BACKGROUND: Pulmonary hypertension (PH) secondary to respiratory disease is caused by pulmonary vascular remodeling and hypoxia. Severe PH can induce various clinical signs, including syncope and right-sided heart failure. HYPOTHESIS/OBJECTIVES: To investigate the echocardiographic characteristics in dogs with PH secondary to respiratory diseases. ANIMALS: Thirty-one dogs with respiratory diseases with or without PH and 15 healthy dogs. METHODS: Prospective cross-sectional study. Dogs were classified according to respiratory disease (obstructive airway/lung disease [OALD] or restrictive lung disease [RLD]) and PH-relevant signs. The association between echocardiographic variables and PH (classified by respiratory disease and PH-relevant signs) was investigated. RESULTS: Twenty-one dogs were diagnosed with PH; of these, 11 showed PH-related signs (OALD, n = 2; RLD, n = 9), 14 had right ventricular hypertrophy, and 19 had pulmonary arterial enlargement. Right ventricular dysfunction and dilatation were observed only in dogs with PH-related signs (n = 10). Left and right ventricular stroke volumes were significantly lower in dogs with PH (median [interquartile range]: 17.2 [12.4-20.8] and 16.8 [15.3-29.5] mL/m2 , respectively). Dogs with RLD had higher echocardiography-estimated pulmonary vascular resistance than those with OALD (median [interquartile range]: 3.1 [1.9-3.3] and 1.6 [1.3-2.2], respectively). CONCLUSIONS AND CLINICAL IMPORTANCE: Pulmonary arterial enlargement was the most common echocardiographic finding in dogs with PH secondary to respiratory diseases. Right ventricular dysfunction, dilatation, and decreased left and right ventricular stroke volume were significantly associated with the PH-related signs, indicating that comprehensive echocardiography is recommended in dogs with respiratory disease. Restricted lung disease might induce more severe PH than OALD.
MISC
88書籍等出版物
20講演・口頭発表等
228担当経験のある科目(授業)
18-
2024年4月 - 現在獣医内科学各論Ⅷ(獣医循環器病学アドバンス)(獣医学科5年次) (日本獣医生命科学大学)
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2023年4月 - 現在獣医内科学各論Ⅱ(獣医循環器病学コア)(獣医学科4年次) (日本獣医生命科学大学)
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2023年4月 - 現在獣医内科学各論Ⅲ(獣医画像診断学)(獣医学科4年次) (日本獣医生命科学大学)
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2022年4月 - 現在獣医救急医療学(獣医学科5年次) (日本獣医生命科学大学)
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2019年4月 - 現在共済実習(獣医学科5年次) (日本獣医生命科学大学)
所属学協会
9-
2023年 - 現在
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2018年 - 現在
共同研究・競争的資金等の研究課題
11-
アニコム損害保険株式会社 共同研究 2022年9月 - 2026年3月
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日本学術振興会 科学研究費助成事業 基盤研究(C) 2022年4月 - 2025年3月
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日本獣医生命科学大学 令和5年度大学間連携等による共同研究 2023年9月 - 2024年3月
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日本獣医生命科学大学 2023年度特色ある研究プロジェクト 2023年8月 - 2024年3月
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令和4 年度大学間連携等による共同研究 2022年9月 - 2023年3月