Curriculum Vitaes
Profile Information
- Affiliation
- Nippon Veterinary and Life Science University
- Degree
- 博士(獣医学)
- J-GLOBAL ID
- 201301091946144408
- researchmap Member ID
- B000234436
Research Areas
1Papers
50-
Journal of Veterinary Medical Science, 2024
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Open Veterinary Journal, 13(12) 1708-1708, 2023Background: We previously reported that myocardial fibrosis may be one of the causes of left ventricular hypertrophy and cardiac dysfunction in dogs with hyperglucocorticism (HGC). The detailed mechanism by which myocardial fibrosis of the left ventricle occurs in dogs with hyperglucocorticism (HGC) remains unclear. Aim: This study investigated the mechanism by which HGC causes fibrosis of the left ventricle.Methods: The impacts of HGC on the heart by comparing samples obtained from high-dose glucocorticoid-treated (P) and untreated (C) dogs. The P group included healthy Beagle dogs (n=6) treated with prednisolone (2 mg/kg, bid, po) for 84 days, and the C group included healthy Beagle dogs (n=6) euthanized for unrelated reasons. In three of the P group dogs, serum was collected before the start of administration (Day 0) and on Day 84 to measure angiotensin II concentrations and oxidative stress markers (8-hydroxy-2’-deoxyguanosine (8OHdG), NADPH oxidase, and superoxide levels). Samples of the left ventricular free wall (LVFW), right ventricular free wall (RVFW), interventricular septum (IVS), and aortic root were harvested from both groups (n = 6 for each group). Using these tissue samples, angiotensin II type 1 receptor (AT1R), 8OHdG, and transforming growth factor β1 (TGFβ1) immunohistochemical stains were performed.Results: The blood NADPH oxidase concentration was significantly higher (P=0.027) in the P group 84 days after initiation of the medication compared to that before prednisolone treatment. By contrast, there was no significant difference in serum angiotensin II (P=0.450), 8OHdG (P=0.068), and superoxide (P=0.057) concentrations. The positive staining rates of AT1R, 8OHdG, and TGFβ1 in the heart (LVFW, RVFW, IVS, and aortic root) were significantly higher in the P group than those in the C group.Conclusion: Angiotensin II and oxidative stress in HGC may cause left ventricular fibrosis in dogs.
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Japanese Journal of Veterinary Anesthesia & Surgery, 54(2) 45-52, 2023
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Open Veterinary Journal, 13(2) 150-150, 2023Background: In recent years, left ventricular hypertrophy and cardiac dysfunction have been reported in human and canine patients with hypercortisolism (HAC) and in dogs treated experimentally with high-dose prednisolone. However, to our knowledge, there have been no reports on the effects of hyperglucocorticism (HGC) on the mitral valve (MV). Aim: This study aimed to compare the MV in dogs treated with high-dose prednisolone with that in healthy dogs to investigate the effects of HGC on the MV.Methods: We investigated the effects of HGC on the MV by comparing samples obtained from high-dose glucocorticoid-treated (P) and healthy (C) dogs. The P group included healthy Beagle dogs (n = 6) treated with prednisolone (2 mg/kg, bid, po) for 84 days and the C group included healthy Beagle dogs (n = 6) euthanized for unrelated reasons. The anterior and posterior mitral leaflets (AML and PML, respectively) from both groups were harvested and stained with hematoxylin–eosin, Alcian blue, and Masson trichome. Additionally, adiponectin (ADN) and glucocorticoid receptor immunohistochemistry were performed. Histological evaluation was performed in the atrialis, spongiosa, fibrosa, and all layers of the proximal, middle, and distal regions of the AML and PML. Results: The proportion of the spongiosa layer thickness to the total thickness was higher in the P than in the C group (proximal and middle AML). However, the proportion of the fibrosa layer thickness to the total thickness was lower in the P than in the C group (middle PML). Areas of acidic sulfated mucosubstance deposition were smaller in the fibrosa layer and all layers (middle AML), while those of collagen deposition were smaller in the spongiosa and total layers (proximal and middle AML), in the P than in the C group. Additionally, ADN expression in the spongiosa layer was higher in the P than in the C group (middle AML). Conclusion: These findings suggest that long-term administration of synthetic glucocorticoids induces histological changes in the MV. These changes may lead to MV dysfunction in dogs with HGC.
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Open Veterinary Journal, 13(2) 150-150, 2023Background: In recent years, left ventricular hypertrophy and cardiac dysfunction have been reported in human and canine patients with hypercortisolism (HAC) and in dogs treated experimentally with high-dose prednisolone. However, to our knowledge, there have been no reports on the effects of hyperglucocorticism (HGC) on the mitral valve (MV). Aim: This study aimed to compare the MV in dogs treated with high-dose prednisolone with that in healthy dogs to investigate the effects of HGC on the MV.Methods: We investigated the effects of HGC on the MV by comparing samples obtained from high-dose glucocorticoid-treated (P) and healthy (C) dogs. The P group included healthy Beagle dogs (n = 6) treated with prednisolone (2 mg/kg, bid, po) for 84 days and the C group included healthy Beagle dogs (n = 6) euthanized for unrelated reasons. The anterior and posterior mitral leaflets (AML and PML, respectively) from both groups were harvested and stained with hematoxylin–eosin, Alcian blue, and Masson trichome. Additionally, adiponectin (ADN) and glucocorticoid receptor immunohistochemistry were performed. Histological evaluation was performed in the atrialis, spongiosa, fibrosa, and all layers of the proximal, middle, and distal regions of the AML and PML. Results: The proportion of the spongiosa layer thickness to the total thickness was higher in the P than in the C group (proximal and middle AML). However, the proportion of the fibrosa layer thickness to the total thickness was lower in the P than in the C group (middle PML). Areas of acidic sulfated mucosubstance deposition were smaller in the fibrosa layer and all layers (middle AML), while those of collagen deposition were smaller in the spongiosa and total layers (proximal and middle AML), in the P than in the C group. Additionally, ADN expression in the spongiosa layer was higher in the P than in the C group (middle AML). Conclusion: These findings suggest that long-term administration of synthetic glucocorticoids induces histological changes in the MV. These changes may lead to MV dysfunction in dogs with HGC.
Misc.
118-
動物臨床医学会年次大会プロシーディング, 32nd(2), 2011
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J Anim Clin Med, 19(1) 1-7, Mar 20, 2010In this study, five cats with systolic heart failure were administered pimobendan, and the effectiveness of the drug was investigated by observing the changes in heart function and clinical symptoms. Two weeks after the drug was given to each dog orally at the dosage of 0.18-0.38 mg/kg twice a day, its effectiveness was evaluated, and compared to the condition of the cats before administration. The left ventricle-fractional shortening, stroke volume, cardiac output, and systolic blood pressure were significantly increased, and the ratio of the left atrium to the aortic root, and the left ventricular diameter at the end of systole were significantly decreased. Pulmonary edema, pleural effusion, and ascites, which had been present before the medication, were resolved, and the condition of the cats was clearly improved according to the New York Heart Association Classification. The mean survival time was prolonged from 52 days to 406 (with median of 203 days). From these results, pimobenndan was judged to have a positive effect on systolic function, reduction of preload, and improvement of quality of life of cats with systolic heart failure.
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動物臨床医学 = Journal of Animal Clinical Research Foundation, 19(1) 1-7, Mar 20, 2010
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Journal of Animal Clinical Medicine, 18(4) 115-119, Dec 25, 2009Patent ductus arteriosus (PDA) was successfully occluded using a coil, and the process was perioperatively observed by transesophageal echocardiography (TEE) in an eight-month papillon which had been diagnosed as suffering from PDA. We were able to confirm the disappearance of the remaining blood flow during the procedure by TEE in addition to auscultation and cardiac angiography. Conventionally confirmation has been done by auscultation during the operation and by cardiac angiography only after coil occlusion, although auscultation is not very reliable. There were no complications due to placing a probe in the esophagus or stomach. The advantages of using TEE for coil occlusion of PDA are real-time monitoring of the condition of the ductus arteriosus, quicker response and higher sensitivity of TEE images than those of cardiac angiography, and continuous monitoring including heart mobility after coil occlusion.
Books and Other Publications
1Teaching Experience
8-
Apr, 2017 - PresentVeterinary general practice III (Nippon Veterinary and Life Science University)
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Apr, 2017 - PresentVeterinary general practice II (Nippon Veterinary and Life Science University)
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Apr, 2017 - PresentVeterinary surgery practiceI-2 (Nippon Veterinary and Life Science University)
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Apr, 2017 - PresentVeterinary surgery practiceI-1 (Nippon Veterinary and Life Science University)
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Apr, 2017 - PresentVeterinary Gastroenterology II (Nippon Veterinary and Life Science University)