Faculty of Veterinary Science
基本情報
- 所属
- 日本獣医生命科学大学 獣医学部獣医学科 教授
- 学位
- 博士(医学)(大阪大学)
- 研究者番号
- 50291159
- J-GLOBAL ID
- 201601010063032190
- researchmap会員ID
- B000250554
- 外部リンク
研究キーワード
30経歴
1-
2017年4月 - 現在
委員歴
11-
2023年9月 - 現在
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2022年6月 - 現在
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2022年4月 - 現在
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2022年4月 - 現在
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2022年4月 - 現在
論文
28-
The Journal of veterinary medical science 2023年2月17日 査読有りThe geneLEAD VIII is a fully-automated nucleic acid extraction/quantitative PCR equipment developed by Precision System Science Co. Ltd, (PSS). To take advantage of its capability, we developed a quantitative assay system to measure growth of animal viruses. The system was used to assay one of the Chinese herbal extracts whose anti-malarial activities were previously reported and demonstrated its dose-dependent anti-viral activity against feline infectious peritonitis virus (FIPV), a feline coronavirus causing the fatal diseases in cats, and relatively low cell toxicity. The assay developed in this study is useful to screen antiviral drugs and the anti-FIPV activity of the herbal extract identified have a potential to lead to development of new drugs against FIPV and other coronaviruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
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Veterinary sciences 9(12) 2022年12月17日 査読有り最終著者Due to the high incidence of mammary tumors in dogs, it is important to elucidate the pathogenesis of these tumors in veterinary medicine. Radiation therapy is often used to treat mammary tumors that target DNA lesions. RAD51 is a key molecule that repairs DNA damage via homologous recombination. We examined the relationship between RAD51 expression and radiosensitivity in mammary tumor cell lines. CHMp and CHMm from the same individual were selected based on the differences in RAD51 expression. The radiosensitivity of both cell lines was examined using MTT and scratch assays; CHMm, which has high RAD51 expression, showed higher sensitivity to radiation than CHMp. However, the nuclear focus of RAD51 during DNA repair was formed normally in CHMp, but not in most of CHMm. Since irradiation resulted in the suppression of cell cycle progression in CHMp, the expression of p21, a cell cycle regulatory factor, was detected in CHMp after 15 Gy irradiation but not in CHMm. These results indicate that functional expression is more important than the quantitative expression of RAD51 in canine mammary tumor cells in response to DNA damage.
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Avian Conservation and Ecology 17(2) 40 2022年11月 査読有り
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Viruses 14(8) 1734-1734 2022年8月6日 査読有り筆頭著者責任著者Feline coronaviruses (FCoVs) infect cats worldwide and cause severe systemic diseases, such as feline infectious peritonitis (FIP). FIP has a high mortality rate, and drugs approved by the Food and Drug Administration have been ineffective for the treatment of FIP. Investigating host factors and the functions required for FCoV replication is necessary to develop effective drugs for the treatment of FIP. FCoV utilizes an endosomal trafficking system for cellular entry after binding between the viral spike (S) protein and its receptor. The cellular enzymes that cleave the S protein of FCoV to release the viral genome into the cytosol require an acidic pH optimized in the endosomes by regulating cellular ion concentrations. Ionophore antibiotics are compounds that form complexes with alkali ions to alter the endosomal pH conditions. This study shows that ionophore antibiotics, including valinomycin, salinomycin, and nigericin, inhibit FCoV proliferation in vitro in a dose-dependent manner. These results suggest that ionophore antibiotics should be investigated further as potential broad-spectrum anti-FCoV agents.
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Oncology reports 47(4) 2022年4月 査読有りHemangiosarcoma (HSA) is a malignant neoplasm that occurs in humans and canines with a poor prognosis owing to metastatic spread, despite effective treatment. The frequency of spontaneous HSA development is higher in canines than in humans. Therefore, canine HSA is a useful model of intractable human disease, which requires early detection and an effective therapeutic strategy. A high frequency of the p110α phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit alpha (PIK3CA) mutations is detected in a comprehensive genome‑wide analysis of canine cases of HSA. The present cloned the full‑length cDNA of canine PIK3CA and identified a mutation in codon 1047 from canine cases of HSA and cell lines that were established from these. The enforced expression of the 1047th histidine residue (H1047)R or L mutants of canine PIK3CA in HeLa cells enhanced epidermal growth factor receptor (EGFR) signaling via Akt phosphorylation. PIK3CA mutant canine HSA cell lines exhibited the hyperphosphorylation of Akt upon EGF stimulation as well. Alpelisib, a molecular targeted drug against PIK3CA activating mutations, exerted a significant antitumor effect in canine PIK3CA‑mutated HSA cell lines. By contrast, it had no significant effect on canine mammary gland tumor cell lines harboring PIK3CA mutations. On the whole, the findings of the present study suggest that alpelisib may be highly effective against PIK3CA mutations that occur frequently in canine HSA.
MISC
22-
広島県獣医学会雑誌 (34) 55-58 2019年7月猫伝染性腹膜炎は有効な薬物治療やワクチンが存在しない致死的な疾患である.今回,胸腹水または末梢血を用いた定量RT-PCR(RT-qPCR)法あるいは剖検材料を用いた免疫染色にてFIPと診断した猫13頭に対して,既存のプレドニゾロン及びインターフェロンによる治療(Pre)と,シクロスポリン(CsA)による治療を行った.CsA療法を行ったウェットタイプでは貯留液の消失が67%で得られたが,平均生存期間の延長は明らかではなかった.ドライタイプでは1例が完全寛解も得られ222日経過した現在も生存中である.またCsA群で誤嚥性肺炎,ウイルス感染性肺炎での死亡例があり,予防策が必要と考えられた.1例のみだが完全寛解が得られたため,CsA療法はドライタイプで治療反応が良い可能性がある.もしそうなら,早期診断早期治療で予後を改善できる可能性があり,まずは治療データの蓄積が必要と考えられる.(著者抄録)
書籍等出版物
1講演・口頭発表等
2担当経験のある科目(授業)
12共同研究・競争的資金等の研究課題
11-
日本学術振興会 科学研究費助成事業 基盤研究(B) 2021年4月 - 2024年3月
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日本学術振興会 科学研究費助成事業 基盤研究(C) 2020年4月 - 2023年3月
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文部科学省 科学研究費補助金(基盤研究(C)) 2014年 - 2016年
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文部科学省 科学研究費補助金(基盤研究(C)) 2010年 - 2012年
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文部科学省 科学研究費補助金(基盤研究(C)) 2007年 - 2008年