Faculty of Applied Life Science 

小林 優多郎

Yutaro KOBAYASHI

基本情報

所属
日本獣医生命科学大学 応用生命科学部 食品科学科 講師
学位
博士(農学)(九州大学)

J-GLOBAL ID
201701016920401931
researchmap会員ID
B000276559

経歴

 3

論文

 20
  • International Congress of Meat Science and Technology 209-210 2024年9月  査読有り
  • International Congress of Meat Science and Technology 181-182 2024年9月  査読有り
  • Yutaro Kobayashi, Masanori Matsuishi
    Japanese Journal of Meat Science and Technology 63 213-213 2022年9月  査読有り
  • Yutaro Kobayashi, Hiroyuki Inagawa, Chie Kohchi, Kimiko Kazumura, Hiroshi Tsuchiya, Toshiyuki Miwa, Katsuichiro Okazaki, Gen-Ichiro Soma
    PLoS ONE 13(6) 2018年6月1日  査読有り
    The pathogenesis of Alzheimer’s disease (AD) remains unclear, but an imbalance between the production and clearance of amyloid-β (Aβ) peptides is known to play a critical role in AD progression. A promising preventative approach is to enhance the normal Aβ clearance activity of brain phagocytes such as microglia. In mice, the intraperitoneal injection of Toll-like receptor 4 agonist was shown to enhance Aβ clearance and exhibit a preventative effect on AD-related pathology. Our previous clinical study demonstrated that orally administered Pantoea agglomerans-derived lipopolysaccharide (LPSp) exhibited an LDL (low-density lipoprotein)-lowering effect in human volunteers with hyperlipidemia, a known risk factor for AD. In vitro studies have shown that LPSp treatment increases Aβ phagocytosis by microglial cells however it is still unclear whether orally administered LPSp exhibits a preventive effect on AD progression. We show here that in senescence-accelerated prone 8 (SAMP8) mice fed a high-fat diet, oral administration of LPSp at 0.3 or 1 mg/kg body weightday for 18 weeks significantly improved glucose metabolism and lipid profiles. The LPSp treatment also reduced pro-inflammatory cytokine expression and oxidative-burst activity in the peripheral blood. Moreover, LPSp significantly reduced brain Aβ burden and memory impairment as seen in the water maze test, although we could not confirm a significant enhancement of Aβ phagocytosis in microglia isolated from the brains after treatment. Taken together, our results show that LPSp holds promise as a preventative therapy for AD or AD-related diseases induced by impairment of metabolic functions.
  • Yutaro Kobayashi, Hiroyuki Inagawa, Chie Kohchi, Kimiko Kazumura, Hiroshi Tsuchiya, Toshiyuki Miwa, Katsuichiro Okazaki, Gen-Ichiro Soma
    PLoS ONE 13(3) e0195008 2018年3月1日  査読有り
    Pantoea agglomerans (P. agglomerans) is a Gram-negative bacterium that grows symbiotically with various edible plants, and the oral or sublingual administration of lipopolysaccharide derived from P. agglomerans (LPSp) have been suggested to contribute to prevention of immune-related diseases. Our previous study indicated that orally administered LPSp was shown to exhibit an LDL-lowering effect in hyperlipidemic volunteers however, a preventive effect of LPSp on atherosclerosis is unclear. The present study attempted to evaluate the anti-atherosclerotic effect by LPSp in a mouse model of high-fat diet (HFD)-induced atherosclerosis. For 16 weeks, apoE-deficient mice were fed an HFD and received drinking water containing LPSp (0.3 or 1 mg/kg body weight/day). The results showed that the orally administered LPSp decreased body weight. A significant reduction in atherosclerotic plaque deposition was observed even with the lower dose of LPSp. The biochemical analyses showed that LPSp markedly improved glucose tolerance and reduced plasma LDL and oxidized LDL levels. In addition, LPSp significantly reduced the production of pro-inflammatory mediators including MCP-1 (in the plasma), TNF-aα and IL-6 (in the colon), and decreased the oxidative burst activities in the peripheral blood sample. Taken together, these results suggest the possibility that oral administration of LPSp can effectively ameliorate HFDinduced hyperlipidemia and inflammatory/oxidative responses to prevent atherosclerosis and related metabolic.

MISC

 2

講演・口頭発表等

 16

担当経験のある科目(授業)

 6

共同研究・競争的資金等の研究課題

 5