研究者業績

原田 泰典

ハラダ ヤスノリ  (Yasunori Harada)

基本情報

所属
兵庫県立大学 大学院 工学研究科 機械系工学専攻 教授
学位
工学博士(1990年11月 広島大学)

J-GLOBAL ID
200901085355792475
researchmap会員ID
1000050633

論文

 331

MISC

 144

書籍等出版物

 14

講演・口頭発表等

 729
  • Y. Inoue, Yoshiko Inoue, Y. Takaue, Yoichi Takaue, M. Takei, Masao Takei, K. Kato, Kazunori Kato, S. Kanai, Sachiyo Kanai, Y. Harada, Yukie Harada, K. Tobisu, Kenichi Tobisu, M. Noguchi, Masanori Noguchi, T. Kakizoe, Tadanobu Kakizoe, K. Itoh, Kyogo Itoh, H. Wakasugi, Hiro Wakasugi
    Journal of Urology 2001年1月1日
    Purpose: Human prostatic acid phosphatase is a prostate specific differentiation antigen. Prostatic acid phosphatase levels increase in the serum of patients with prostate cancer and its peptide from positions 299 to 307 (PAP 299-307) is recognized by HLA-A2 restricted cytotoxic T lymphocytes. We investigated whether HLA-A2402 binding prostatic acid phosphatase derived peptides induce HLA-A2402 restricted, tumor specific cytotoxic T lymphocytes from the peripheral blood mononuclear cells of patients with prostate cancer. Materials and Methods: Peptide binding activity was measured with RMA-S-A*A2402 cell lines and flow cytometry. Cytotoxic T-lymphocyte activity of the peripheral blood mononuclear cells of patients with prostate cancer and healthy donors was measured by interferon-γ and51creatinine release assays. Prostatic acid phosphatase expression in the tumor cell lines at the messenger RNA and protein levels was investigated by reverse transcriptase-polymerase chain reaction and immunohistochemical analysis, respectively. Results: An HLA-A2402 binding, prostatic acid phosphatase derived peptide consisting of the prostatic acid phosphatase amino acid sequence from positions 213 to 221 (PAP 213-221, LYCESVHNF) showed the ability to induce HLA-A2402 restricted and tumor specific cytotoxic T lymphocytes, which are cytoxic to prostatic acid phosphatase positive tumor cells from the peripheral blood mononuclear cells of patients with prostate cancer. Conclusions: PAP 213-221 may be appropriate as a cancer vaccine for specific immunotherapy in patients with HLA-A2402 positive prostate cancer.
  • 牧 清二郎, 京増 泰範, 原田 泰典, 森 謙一郎
    塑性と加工 2000年11月25日
  • 牧 清二郎, 辻 信彦, 原田 泰典, 中村 雅勇
    塑性と加工 2000年11月25日
  • 原田 泰典, 大森 正信
    材料とプロセス : 日本鉄鋼協会講演論文集 = Current advances in materials and processes : report of the ISIJ meeting 2000年9月1日
  • 原田 泰典, 森 謙一郎, 牧 清二郎, 竹田 仁, 太田 訓郎, 瀬野尾 和久
    材料とプロセス : 日本鉄鋼協会講演論文集 = Current advances in materials and processes : report of the ISIJ meeting 2000年9月1日
  • 原田 泰典, 森 謙一郎, 福岡 義晃, 牧 清二郎
    塑性と加工 2000年3月25日
  • 牧 清二郎, 羽根田 修, 原田 泰典, 中村 雅勇
    塑性と加工 2000年2月25日
  • 日本塑性加工学会連合講演会講演論文集 2000年
  • 日本塑性加工学会連合講演会講演論文集 2000年
  • 日本塑性加工学会連合講演会講演論文集 2000年
  • 日本塑性加工学会春季講演論文集 2000年
  • 日本鉄鋼協会講演論文集, 材料とプロセス 2000年
  • 日本塑性加工学会春季講演論文集 2000年
  • 日本塑性加工学会春季講演論文集 2000年
  • 日本塑性加工学会春季講演論文集 2000年
  • 日本塑性加工学会春季講演論文集 2000年
  • Norio Nonomura, Norio Nonomura, Yutaka Ono, Masahiro Nozawa, Tatsunari Fukui, Yasunori Harada, Kazuo Nishimura, Natsuki Takaha, Shiro Takahara, Akihiko Okuyama
    European Urology 2000年1月1日
    Objectives: The aim of this study is to evaluate the efficacy and safety of intrarenal bacillus Calmette-Guerin (BCG) instillation as a treatment for transitional cell carcinoma in situ (CIS) of the upper urinary tract. Methods: Diagnostic criteria of upper urinary tract CIS were (1) positive urinary cytology, (2) negative multiple random biopsy of the bladder and prostatic urethra, (3) negative radiographic findings in the upper urinary tract and (4) two serial positive cytologies in selective ipsilateral urine sampling from the pyeloureteral system. Eleven patients diagnosed as having upper urinary tract CIS were enrolled in this study. Thus, 11 renal units were treated with BCG instillation. After placing a 6-french Double-J stent, BCG (80 mg) in 40 ml saline was instilled into the bladder weekly, 6 times in total as one course. Results: At the end of one course, 9 cases showed negative urinary cytology. Among these 9 cases, 2 showed recurrence in the upper urinary tract after 4 months and 8 months of disease-free interval, respectively. These 2 cases have received an additional course of BCG instillation, but the urinary cytology did not normalize. Mean recurrence-free time was 19.6 months. Of the other 7 cases who responded to the first course of instillation, 6 cases were alive with no evidence of the disease. The remaining patient died of rectal cancer with no evidence of transitional cell carcinoma (TCC). Of the 2 cases who showed positive urinary cytology even after the first course, 1 underwent nephroureterectomy. The other case was diagnosed as having malignant lymphoma 3 months after the end of this instillation therapy, and he died of malignant lymphoma. As side effects, 8 cases (72.7%) showed bladder irritability, and 4 presented fever higher than 38°C. However, no patient needed antitubercular treatment. Conclusion: As for the short-term response, BCG instillation for the treatment of upper urinary tract CIS is considered to be effective and safe. Longer follow-up and further experience with this treatment are required. Copyright (C) 2000 S. Karger AG, Basel.
  • Norio Nonomura, Kazuo Nishimura, Yutaka Ono, Tatsunari Fukui, Yasunori Harada, Natsuki Takaha, Shiro Takahara, Akihiko Okuyama
    Urology 2000年1月1日
    Objectives. Fas/APO-1 is an apoptosis-signaling cell-surface receptor belonging to the tumor necrosis factor receptor family. The Fas-Fas ligand system plays an important role in cytotoxic T-lymphocyte-mediated or natural killer cell-mediated cytotoxicity against tumor cells. Soluble Fas (sFas), generated by alternative splicing, has been reported to antagonize the interaction of cell-surface Fas with Fas ligand. This study examined the level of sFas in the serum of patients with renal cell carcinoma (RCC) and investigated the correlation between the sFas level and clinicopathologic parameters of RCC. Methods. Using reverse transcriptase-polymerase chain reaction, we examined the production of sFas messenger RNA (mRNA) from the cultured human RCC cell lines ACHN and OUR-10 and from surgical specimens. We also measured sFas levels in the serum of 31 patients with RCC before and after nephrectomy using an sFas-specific enzyme-linked immunosorbent assay. Results. mRNA of sFas was identified both in cultured ACHN cells and human RCC tissues, although mRNA of wild-type Fas was exclusively predominant. The level of sFas in the serum of patients with RCC was significantly higher than that of normal controls, but sFas was not detectable in the supernatant of cultured renal cancer cells. Preoperative and postoperative serum sFas levels did not clearly correlate with the patients' age or sex or with histologic stage, grade, or cell type of RCC. The serum sFas level in patients with RCC correlated with tumor size. In 24 of the 31 cases, radical nephrectomy reduced the serum sFas level within 3 months.Conclusions. Our results suggest that the elevated serum sFas level in patients with RCC might not be derived from the tumor itself but might reflect an immune response to the tumor burden. Serum sFas may be a useful indicator of tumor burden in patients with RCC. Copyright (C) 2000 Elsevier Science Inc.
  • Y. Harada, N. Nonomura, K. Nishimura, H. Tamaki, S. Takahara, T. Miki, H. Sugiyama, A. Okuyama
    Molecular Urology 1999年12月1日
    The Wilms' tumor 1 gene (WT1) is a tumor suppressor gene whose alterations are linked to the genesis of Wilms' tumor. The gene is expressed in the urogenital organs and plays a key role in their development. Recent studies have shown that the WT1 gene product acts as a growth promoter of human leukemic cells. Because WT1 has been reported to be important in testicular development, we have investigated WT1 messenger RNA (mRNA) expression in testicular germ-cell tumors. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the levels of WT1 mRNA in 34 patients with testicular germ-cell tumor, including 25 low-stage and 9 high-stage tumors. There were 23 seminomas and 11 nonseminomas. The WT1 mRNA was highly expressed in 6 of 9 high-stage lesions (67%) but only 5 of 25 low-stage cases (20%). A significant correlation was observed between the extent of WT1 mRNA expression and tumor stage (P = 0.017). There was no significant difference in WT1 mRNA expression between seminomas and nonseminomatous tumors. These results suggest that WT1 may be causal for the progression of testicular germ-cell tumors.
  • 原田 泰典, 森 謙一郎, 牧 清二郎
    塑性と加工 1999年11月
  • 牧 清二郎, 原田 泰典, 中村 雅勇
    塑性と加工 1999年11月
  • 原田 泰典, 森 謙一郎, 牧 清二郎
    塑性と加工 1999年9月
  • 原田 泰典, 森 謙一郎, 牧 清二郎, 竹田 仁, 太田 訓郎, 中野 智弘
    材料とプロセス : 日本鉄鋼協会講演論文集 = Current advances in materials and processes : report of the ISIJ meeting 1999年9月1日
  • Y. Harada, N. Nonomura, M. Kondo, K. Nishimura, S. Takahara, T. Miki, A. Okuyama
    European Urology 1999年8月23日
    Objectives: To evaluate the natural history and the efficacy of treatments for renal cell carcinoma (RCC) with brain metastasis, we reviewed 18 patients with this disease. Methods: Out of 325 cases with RCC treated at Osaka University Hospital from 1957 to 1993, 18 15.5%, male:female ratio 16:2) cases developed brain metastases. Median follow-up was 44 months after the initial treatment of the primary lesion. Twelve patients had surgical resection of brain metastases (surgical group), and 7 of them received adjuvant radiotherapy. Six patients with poor performance status were treated with supportive therapy alone (nonsurgical group). Results: Of 18 RCC patients with brain metastasis, 16 were male and 2 female. All brain metastases except for 1 case were symptomatic. Median interval between the initial treatment of the primary lesion and the diagnosis of brain metastasis was 19 months. The most frequent metastatic site prior to brain was the lung, which was detected in 7 cases (38.9%). Median survival of the entire group, measured from the onset of brain metastasis, was 9.5 months. One-year survival rate after the diagnosis of brain metastasis was 43.2% (64.8% in surgical group, 0% in nonsurgical group), 3-year 18.5% and 5-year 0%. Among 109 metastatic RCC, 14 patients were treated by lymphokine-activated killer (LAK) therapy. Out of 14 metastatic RCC patients treated by LAK therapy, 3 (21.4%) developed brain metastases. On the other hand, out of 95 metastatic RCC patients without LAK therapy, 15 (15.8%) had brain metastases. There was no significant difference in the rate of brain metastases between these two groups. Conclusion: There was a trend for prognosis of the surgical group to be better compared to that of the nonsurgical group, although it is not statistically significant. The optimum treatment for brain metastasis of RCC remains undefined, but our data suggested surgical resection in selected patients might contribute to prolonged survival of patients with brain metastasis. LAK therapy was not necessarily the risk factor of the brain metastasis.
  • N. Nonomura, T. Imazu, Y. Harada, M. Nozawa, Y. Ono, T. Fukui, K. Nishimura, A. Okuyama
    Hinyokika kiyo. Acta urologica Japonica 1999年8月1日
    Most testicular germ cell tumors have serological tumor markers such as alpha-fetoprotein (AFP) and human chorionic gonadotropin beta-subunit (beta-HCG). On the other hand, molecular staging of these tumors has not been well established compared to other urogenital malignancies like prostate cancer. Recently, melanoma antigen (MAGE) which is one of the tumor-associated antigens recognized by cytotoxic T lymphocytes (CTL) has been found to be present in a variety of malignant tumor types and normal testis. In addition, Wilms' tumor-associated gene WT1 has been found to be expressed in some testicular cancers. Thus, we examined the expression of these genes in testicular cancer tissues and peripheral blood of cancer-bearing patients using reverse transcription-polymerase chain reaction (RT-PCR). The expression of the MAGE1-3 genes was examined in 34 testicular germ cell tumors (24 seminomas and 10 nonseminomas). Of the seminomas and nonseminomas, 87.5% and 40% were positive for MAGE1, 91.7% and 60% for MAGE2, and 83.3% and 30% for MAGE3, respectively. The expression of the MAGE genes was not correlated with the tumor stage. The expression of the WT1 gene was quantified in 26 testicular germ cell tumors. WT1 was highly expressed in 5 of the 7 high stage cases, but in only 4 of the 19 low stage cases (p < 0.01). The mRNA of these genes could not be detected from the peripheral blood of patients with high stage tumors. These results suggest that MAGE genes may be useful tumor markers for molecular staging of testicular cancer, especially seminoma, and that the WT1 gene may be a tumor marker for high stage testicular germ cell tumors. However, these genes cannot yet be used for molecular staging of testicular germ cell tumors.
  • 原田 泰典, 森 謙一郎, 牧 清二郎, 中村 雅勇
    日本機械学會論文集. C編 1999年7月25日
    A method for refining grains near the surfaces of metal workpieces using hot shot peening is proposed to improve the surface properties of the workpiece. In this method, the grains are refined due to plastic deformation generated by the hit of many shots under hot working conditions. A model experiment using two shots is carried out to examine the effects of the amount of deformation, the processing temperature and the interval of the hit on the grain size. In the experiment, the workpieces are stainless steel SUS304, carbon steel S25C, mild steel and commercially pure copper. By means of p...
  • 牧 清二郎, 原田 泰典, 中村 雅勇
    材料試験技術 1999年4月
  • 日本塑性加工学会連合講演会講演論文集,第50回 1999年
  • 日本塑性加工学会連合講演会講演論文集,第50回 1999年
  • 日本塑性加工学会春季講演論文集 1999年
  • 日本塑性加工学会春季講演論文集 1999年
  • 牧 清二郎, 広岡 良顕
    材料試験技術 1999年1月
  • 原田 泰典, 森 謙一郎, 牧 清二郎, 中村 雅勇
    日本機械学会論文集C編 1999年
    A method for refining grains near the surfaces of metal workpieces using hot shot peening is proposed to improve the surface properties of the workpiece. In this method, the grains are refined due to plastic deformation generated by the hit of many shots under hot working conditions. A model experiment using two shots is carried out to examine the effects of the amount of deformation, the processing temperature and the interval of the hit on the grain size. In the experiment, the workpieces are stainless steel SUS304, carbon steel S25C, mild steel and commercially pure copper. By means of peening with many shots, the global surface layer successfully attains to the fine grains.
  • Fusahito Yoshida, Tatsuo Okada, Misao Itoh, Yasunori Harada, Masanobu Ohmori
    Metals and Materials International 1998年12月1日
    The present paper describes how the cladding of chromium plate with dissimilar metals improves the plastic bendability of the chromium. Three-point bending tests at various temperatures were performed for three types of chromium specimens: a monolithic chromium plate, aluminium- and steel-clad chromium plate. The aluminium-clad chromium plate was bent at 343 K up to a bent angle of 90 degrees without failure, even when the chromium layer was located outside of the plate (tension side), while the monolithic chromium plate could be bent exclusively at temperatures above 403 K. When the chromium layer was located inside of the steel-clad chromium plate (compression side), the plate was successfully bent at 307 K. The FE stress analysis of bending proved that the cladding of chromium plates with proper metals of different kinds is effective to reduce the tensile stress in chromium induced during bending and also the residual stress existing after bending operation.
  • 牧 清二郎
    大会講演概要 1998年11月9日
  • 原田 泰典, 大森 正信, 安藤 博紀, 松井 英則, 沖田 智樹
    日本金屬學會誌 1998年9月20日
  • Yasunori Harada, Hideya Kuroda, Toshinobu Seguchi, Makoto Higashino, Hiroyuki Senoh, Masahiko Tsujimoto
    Acta Urologica Japonica 1998年4月1日
    A 55-year-old male visited our hospital with a complaint of gross hematuria and right lower abdominal pain. Cytological findings of voided urine suggested the presence of malignant cells. Cystoscopic examination revealed bloody urine discharge from the right ureteral orifice and no abnormality in the bladder wall. The retrograde pyelogram showed no tumor masses. However, malignant cells were detected cytologically in the right ureteral catheteral urine twice. Under the preoperative diagnosis of primary urothelial tumor of the right upper urinary tract, right total nephroureterectomy was performed. A histological study revealed transitional cell carcinoma in situ in the lower portion of the ureter. We reviewed 46 cases of primary carcinoma in situ of the upper urinary tract previously reported in Japan.
  • 原田 泰典
    弓削商船高等専門学校紀要 1998年2月27日
    Shot peening, one of the surface treatment method, has widely applied to exert a positive influence on fatigue by the plastic deformation and the compressive residual stress of surface layers. In previous study, it revealed that hot shot peening caused the refinement in size and homogeneity of the crystal grains just under the treated surface. Therefore, shot peening was carried out in hot atmosphere aiming at the improvement of surface properties of metallic materials. Especially, the present shot peening was examined lining of metal plates with foils, since lining was avaible for improvin...
  • 日本塑性加工学会連合講認会講演論文集,第49回 1998年
  • 日本塑性加工学会連合講演会講演論文集,第49回 1998年
  • 日本学術振興会先端材料技術第156委員会講演会,第16回 1998年
  • 日本塑性加工学会春季講演論文集 1998年
  • 日本塑性加工学会春季講演論文集 1998年
  • 日本塑性加工学会春季講演論文集 1998年
  • 日本塑性加工学会春季講演論文集 1998年

担当経験のある科目(授業)

 18

共同研究・競争的資金等の研究課題

 20