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  2. 山内 大輔

山内 大輔

ヤマウチ ダイスケ  (Daisuke Yamauchi)

基本情報

所属
兵庫県立大学 大学院理学研究科 准教授
学位
博士(理学)(東京都立大学)
理学修士(東京都立大学)
J-GLOBAL ID
200901080373900437
researchmap会員ID
1000053925

研究キーワード

 4

研究分野

 1

経歴

 6
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学歴

 4

委員歴

 3

主要な論文

 38
  • 山内大輔, 福田安希, 唐原一郎, 峰雪芳宣
    Plant Morphology 28 3-7 2016年  査読有り招待有り
  • Keita Sutoh, Kenji Washio, Ryozo Imai, Masamitsu Wada, Tomonori Nakai, Daisuke Yamauchi
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 79(5) 747-759 2015年5月  査読有り
    The expression of the gene for a proteinase (Rep1) is upregulated by gibberellins. The CAACTC regulatory element (CARE) of the Rep1 promoter is involved in the gibberellin response. We isolated a cDNA for a CARE-binding protein containing a Myb domain in its carboxyl-terminal region and designated the gene Carboxyl-terminal Myb1 (CTMyb1). This gene encodes two polypeptides of two distinctive lengths, CTMyb1L and CTMyb1S, which include or exclude 213N-terminal amino acid residues, respectively. CTMyb1S transactivated the Rep1 promoter in the presence of OsGAMyb, but not CTMyb1L. We observed an interaction between CTMyb1S and the rice prolamin box-binding factor (RPBF). A bimolecular fluorescence complex analysis detected the CTMyb1S and RPBF complex in the nucleus, but not the CTMyb1L and RPBF complex. The results suggest that the arrangement of the transfactors is involved in gibberellin-inducible expression of Rep1.
  • Daisuke Yamauchi, Daisuke Tamaoki, Masato Hayami, Miyuki Takeuchi, Ichirou Karahara, Mayuko Sato, Kiminori Toyooka, Hiroshi Nishioka, Yasuko Terada, Kentaro Uesugi, Hidekazu Takano, Yasushi Kagoshima, Yoshinobu Mineyuki
    Journal of Electron Microscopy 62(3) 353-361 2013年6月  査読有り
    The cotyledon of legume seeds is a storage organ that provides nutrients for seed germination and seedling growth. The spatial and temporal control of the degradation processes within cotyledons has not been elucidated. Calcium oxalate (CaOx) crystals, a common calcium deposit in plants, have often been reported to be present in legume seeds. In this study, micro-computed tomography (micro-CT) was employed at the SPring-8 facility to examine the three-dimensional distribution of crystals inside cotyledons during seed maturation and germination of Lotus miyakojimae (previously Lotus japonicus accession Miyakojima MG-20). Using this technique, we could detect the outline of the embryo, void spaces in seeds and the cotyledon venation pattern. We found several sites that strongly inhibited X-ray transmission within the cotyledons. Light and polarizing microscopy confirmed that these areas corresponded to CaOx crystals. Three-dimensional observations of dry seeds indicated that the CaOx crystals in the L. miyakojimae cotyledons were distributed along lateral veins however, their distribution was limited to the abaxial side of the procambium. The CaOx crystals appeared at stage II (seed-filling stage) of seed development, and their number increased in dry seeds. The number of crystals in cotyledons was high during germination, suggesting that CaOx crystals are not degraded for their calcium supply. Evidence for the conservation of CaOx crystals in cotyledons during the L. miyakojimae germination process was also supported by the biochemical measurement of oxalic acid levels. © The Author 2012. Published by Oxford University Press [on behalf of Japanese Society of Microscopy]. All rights reserved.
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MISC

 72
  • Keita Sutoh, Daisuke Yamauchi, Takao Minamikawa, Hideki Kato
    Plant Biotechnology 20(2) 113-119 2003年  
    A gene for a cysteine endopeptidase REP-1, Repl, is expressed in both the embryo and aleurone layers in germinating rice seeds. The expression of Repl was not repressed in the embryo by exogenously applied uniconazole, a gibberellin (GA) biosynthesis inhibitor, even though GA-inducible expression of the gene occurred in the aleurone layers. Serially truncated Repl promoters fused to the β-glucuronidase (GUS) reporter gene were employed to identify the cis-regulatory elements involved in the embryo-specific expression. The deletion of the region from-500 to-432 decreased the GUS activity significantly. Linker-scan mutation analysis was carried out to determine the sequences involved in the expression of Repl in embryos, which showed that the region from-500 to-432 contained at least two positive regulatory elements. © 2003, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.
  • D Yamauchi
    PLANT AND CELL PHYSIOLOGY 42(6) 635-641 2001年6月  
    alpha -Amylase is expressed at high levels in cotyledons of germinated seeds of Vigna mungo. The mRNA for alpha -amylase appeared in cotyledons of the seeds at id after imbibition started (DAI). Two TGACGT motifs at -445 and at -125 in the promoter region of the gene interacted with nuclear proteins from cotyledons of dry seeds and the activities were detected until 3 DAI, A transient assay with particle bombardment showed that the downstream region from -135 in the promoter was required for high level expression in the cotyledons and the activity was reduced by mutation of the TGACGT motif at -125, The activities to bind the TGACGT motifs were detected in the axes of the seeds at 1 DAI but disappeared at 4 DAI, although the mRNA for a-amylase in the axes appeared at 4 DAI and increased in level by 6 DAI, A transient assay experiment showed that a positive regulatory element for the expression in the axes was located in the region from -630 to -453, These results indicated that the TCACGT motif at -125 was required for high level expression of the gene in the cotyledons of the germinated seeds.
  • Daisuke Yamauchi
    Plant Biotechnology 17(3) 263-267 2000年  
    A gene for Brazil nut 2S albumin, a methionine-rich protein, was fused to a promoter region of the canavalin gene. This chimeric gene was introduced into shoot apices of embryonic axes of French bean by particle bombardment and seeds were obtained from plants regenerated from the bombarded axes. Protein in the transgenic seeds was analyzed by immunoblotting with an antiserum against Brazil nut 2S albumin. A 12-kDa immunoreactive polypeptide was detected and its amount in the seed was estimated to comprise less than 1% of the soluble protein.
  • PY Zhong, D Yamauchi, T Okamoto, T Okabe, T Minamikawa
    PLANTA 210(1) 72-79 1999年11月  
    Pod storage protein (PSP) accumulated in developing pods of French bean (Phaseolus vulgaris L.) plants, and increasing the PSP mRNA level by pod removal resulted in the enhancement of PSP accumulation in pods that formed later. Pod storage protein tvas detected in flowers, young leaves and young stem internodes in addition to pods. Accumulation of PSP and its mRNA was induced by sink-removal in an organ-specific manner. In addition, wounding induced PSP accumulation systemically in leaves. Methyl jasmonate did not induce PSP synthesis but enhanced the synthesis that was induced by wounding. In senescing pods, PSP was degraded, and degradation products with molecular masses of 20 and 17 kDa were detected in the pods. The amount of 20-kDa degradation product was greater than that of the 17 kDa product.
  • D Yamauchi, T Minamikawa
    JOURNAL OF PLANT RESEARCH 111(1101) 1-6 1998年3月  
    Legume seeds contain a large amount of proteins and are one of the essential protein sources for humans and animals. However, the protein in legume seeds is usually poor in sulfur-containing amino acids, and its nutritional value is lower than the protein from animal sources. Recently plant breeding has become available by the introduction of molecular biology, and a technique, called molecular breeding, was applied to the production of legume seeds that contain proteins with high nutritional quality. This review describes the expression of legume seed protein genes and the transformation of legume plants. Approaches to improve the legume seed storage protein will be discussed.
  • D Yamauchi
    PLANT SCIENCE 126(2) 163-172 1997年8月  
    To investigate trans-acting elements interacting with the upstream region of the canavalin gene from Canavalia gladiata (Japanese jack bean), gel retardation assays were carried out with the crude nuclear extract from immature C. gladiata embryos. Results of DNaseI footprinting experiments indicated that some proteins interacted with A/T-rich sequences in the upstream region of the gene. Other proteins bound to three sites containing CA-rich sequences in the upstream region, and competition assays suggested that the proteins were similar to each other. Results of the deletion analysis of the upstream region of the gene suggest that one site of them is involved in the negative regulation of its expression. (C) 1997 Elsevier Science Ireland Ltd.
  • PY Zhong, T Tanaka, D Yamauchi, T Minamikawa
    PLANT PHYSIOLOGY 114(3) 724-724 1997年7月  
  • PY Zhong, T Tanaka, D Yamauchi, T Minamikawa
    PLANT PHYSIOLOGY 113(2) 479-485 1997年2月  
    When French bean (Phaseolos vulgaris) plants were depodded in the early stages of fruit development, relative levels of a specific protein with a relative molecular weight of 28,000 were enhanced in the young pods that formed later. The protein, designated pod storage protein (PSP), was purified from extracts of newly formed pods from plants that had been previously depodded four times at intervals of 2 weeks. Two-dimensional polyacrylamide gel electrophoresis showed the presence of three forms (designated A, B, and C) of PSP with identical electrophoretic mobilities but different charges. The molecular mass of native PSP was estimated by gel filtration to be 67 kD; therefore, the protein was most likely present as a dimer. The antisera raised against forms A and C were crossreactive with each other. Form B lacked the N-terminal alanine of forms A and C. An expression library from French bean pods was screened using the antiserum against form A, and a full-length cDNA clone was isolated. The cDNA insert included 765 bp potentially encoding a polypeptide with 255 amino acid residues (and a calculated molecular mass of 28,854 D). The amino acid sequence deduced from the PSP cDNA had 65 to 71% identity with soybean (Glycine max) vegetative storage protein sequences (P.E. Staswick [1988] Plant Physiol 87: 250-254; and Correction [1989] Plant Physiol 89: 717). Genomic Southern blot analysis suggested that PSP is derived from a single-copy gene.
  • D Yamauchi, Y Terasaki, T Okamoto, T Minamikawa
    PLANT MOLECULAR BIOLOGY 30(2) 321-329 1996年1月  
    Cysteine endopeptidases, SH-EP from Vigna mungo and EP-C1 from Phaseolus vulgaris, act to degrade seed storage protein during seed germination. Using transgenic tobacco plants, expression of SH-EP and promoter activity of the EP-C1 gene were analyzed in transgenic tobacco plants. The promoters of the two genes in tobacco seeds showed germination-specific activation, although post-translational processing of SH-EP and regulatory regions of promoter of the gene for EP-C1 were found to differ between leguminous seeds and transgenic tobacco seeds.
  • Y TERASAKI, D YAMAUCHI, H MORIKAWA, T MINAMIKAWA
    PLANT AND CELL PHYSIOLOGY 36(3) 537-541 1995年4月  
    Levels of a cysteine endopeptidase, EP-C1, and its mRNA increased slightly in cotyledons of French bean detached from embryonic axes. A transient expression assay by a particle gun indicated that the downstream region from position -340 of the EP-C1 gene was sufficient for gene expression in cotyledons of germinating seeds.
  • S YAMAMOTO, M NISHIHARA, H MORIKAWA, D YAMAUCHI, T MINAMIKAWA
    PLANT MOLECULAR BIOLOGY 27(4) 729-741 1995年2月  
    A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion mutants and examined the transient expression of beta-glucuronidase reporter gene by particle bombardment and the stable expression by Agrobacterium-mediated transformation of tobacco plants. Positive regulatory elements were located in the -894/-602 and -602/-74 regions of the Con A gene, and in the -428/-376, -281/-155 and -155/-50 regions of the canavalin gene. In addition, the results suggested that the A/T-rich sequences in the 5'-upstream region of the Con A gene play a role in transcriptional activation, but that those of the canavalin gene have little effect on the gene expression. The CATGCAT/A sequence was not sufficient by itself for high levels of expression of both the Con A and canavalin genes. The canavalin polypeptide amounted to about 1% of the total extractable protein in the transgenic tobacco seeds, but the Con A polypeptide was not detected in the extractable protein.
  • 種子のバイオサインエンス,学会出版センター 71-78 1995年  招待有り
  • D YAMAUCHI, H TAKEUCHI, T MINAMIKAWA
    PLANT AND CELL PHYSIOLOGY 35(4) 705-711 1994年6月  
    A single copy of the alpha-amylase gene, composed of three introns and four exons, was found in Vigna mungo. Examination of levels of alpha-amylase and its mRNA in detached cotyledons indicated that attachment of the embryonic axis is not required for expression of the gene in cotyledons of germinating seeds.
  • 山内大輔
    植物細胞工学 5(3) 174-183 1993年5月  招待有り
  • Y OGUSHI, T TANAKA, D YAMAUCHI, T MINAMIKAWA
    PLANT MOLECULAR BIOLOGY 19(4) 705-706 1992年7月  
  • D YAMAUCHI, T MINAMIKAWA
    NUCLEIC ACIDS RESEARCH 18(14) 4250-4250 1990年7月  
  • H AKASOFU, D YAMAUCHI, T MINAMIKAWA
    NUCLEIC ACIDS RESEARCH 18(7) 1892-1892 1990年4月  
  • D YAMAUCHI, T MINAMIKAWA
    FEBS LETTERS 260(1) 127-130 1990年1月  
  • D YAMAUCHI, K NAKAMURA, T ASAHI, T MINAMIKAWA
    PLANT AND CELL PHYSIOLOGY 30(1) 147-150 1989年1月  
  • Daisuke YAMAUCHI, Kenzo NAKAMURA, Tadashi ASAHI, Takao MINAMIKAWA
    European Journal of Biochemistry 170(3) 515-520 1988年  
    By a method of Escherichia coli expression‐vector‐primed cDNA synthesis, a cDNA expression library was constructed from total poly(A)‐rich RNA that was prepared from immature embryos of Canavalia gladiata. Essentially full‐length cDNA clones for two seed proteins, canavalin and concanavalin A, were selected from the library by immunological screening of the colonies and in vitro RNA synthesis and translation. The complete amino acid sequence of canavalin was determined from the nucleotide sequence of the corresponding cDNA and was found to be very homologous to 7S seed proteins of other legumes. The nucleotide sequence of the cDNA predicts a 26‐amino‐acid extension in the precursor at the amino terminus of the mature canavalin. Canavalin mRNA and concanavalin A mRNA levels at successive stages of the seed development were estimated by RNA blot hybridization and results indicated that the two mRNA levels are differently regulated. Copyright © 1988, Wiley Blackwell. All rights reserved
  • D YAMAUCHI, T MINAMIKAWA
    PLANT AND CELL PHYSIOLOGY 28(3) 421-430 1987年4月  
  • D YAMAUCHI, T MINAMIKAWA
    PLANT AND CELL PHYSIOLOGY 27(6) 1033-1041 1986年9月  

書籍等出版物

 1

講演・口頭発表等

 5

担当経験のある科目(授業)

 4

所属学協会

 4

共同研究・競争的資金等の研究課題

 11
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