Masahiro Takeo, Isao Kawaji, Akihiro Nakasuji, Takaaki Tone, Yoshiaki Ukita, Dai Ichiro Kato, Seiji Negoro, Shinichi Yusa, Makoto Katayama, Yuichi Utsumi
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 1192-1194 2012年1月1日
We developed an automatic analytical system for the determination of an endocrine disrupting chemical, nonylphenol (NP), which employs a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA) using a novel antigen-bound microfilter. The filter was set in a unique 3-dimensional microreactor, and all the fluidic processes for the reaction and detection were automatically performed according to a PC program in the system. By using this system, we obtained a negative standard curve, of which NP concentration range (0.01-0.40 μg/L) was much lower than the quantitative limit (5 μg/L) of a conventional ELISA kit for NP measurement. The coefficients of the variation were comparable to those of commercial products.