研究者業績

中村 光伸

ナカムラ ミツノブ  (Mitsunobu Nakamura)

基本情報

所属
兵庫県立大学 大学院 工学研究科 准教授
学位
博士(工学)

J-GLOBAL ID
200901063499685190
researchmap会員ID
1000214809

外部リンク

研究キーワード

 1

論文

 91
  • Tadao Takada, Nao Shimogaki, Moe Naruo, Mitsunobu Nakamura, Kazushige Yamana
    ChemPhotoChem 6(8) 2022年8月  
    The molecular arrangement of functional chromophores is essential to construct functional nanomaterials. Synthetic DNA has been used as a structural scaffold to control and construct molecular assembly. We now describe the formation of artificial DNA/porphyrin complexes where porphyrins working as photoactive molecules were placed at specific locations on the DNA through a non-covalent interaction. Spectroscopic analysis by UV/vis, circular dichroism (CD), and melting temperature measurements showed that the binding of water-soluble porphyrin derivatives with methylpyridinium groups (TMPyP and DMPyP) to DNA can be directed by hydrophobic cavities composed of a pair of abasic site analogs (dS). CD measurements showed that the two porphyrins can be accommodated at two specific sites when the DNA molecule had two cavities, as evidenced by the exciton-coupled CD spectra of the porphyrins. Photoelectrochemical experiments showed that the DNA complexes accommodating DMPyP at specific locations can respond to light excitation to generate a photocurrent when immobilized on the electrode surface. Our results demonstrated that the control of the porphyrin binding using the dS/dS pair is a useful approach to construct artificial DNA with porphyrin molecules, leading to the design of photoresponsive materials and photoelectrochemical sensors.
  • Tadao Takada, Koma Nishida, Yurika Honda, Aoi Nakano, Mitsunobu Nakamura, Shuya Fan, Kiyohiko Kawai, Mamoru Fujitsuka, Kazushige Yamana
    ChemBioChem 22(17) 2729-2735 2021年9月2日  
  • Mitsunobu Nakamura, Tadao Takada, Kazushige Yamana
    ChemBioChem 20(23) 2949-2954 2019年  査読有り
    B- to Z-DNA transitions play a crucial role in biological systems and have attracted the interest of researchers for their applications in DNA nanotechnology. DNA and DNA analogues have also been used as templates to construct helical chromophore associations with π interactions. In this work, the B- to Z-DNA transition-induced switching of pyrene in an association manner was evaluated using DNA duplexes with non-nucleosidic pyrene residues in the middle of d(CG) repeat sequences. One of the pyrene-labeled DNAs was shown to exhibit inverted exciton coupled circular dichroism signals upon pyrene association through a B- to Z-DNA transition. This observation indicates that pyrene association switches the DNA conformation from right- to left-handed. Interestingly, the fluorescence of the pyrene-labeled DNA duplex also dynamically changed upon switching of the pyrene in an association-based manner. Taken together, these studies demonstrate that pyrene-labeled DNA shows promise as a chiroptical molecular switch.
  • Mitsunobu Nakamura, Yuki Matsui, Tadao Takada, Kazushige Yamana
    ChemistrySelect 4 1525-1529 2019年  査読有り
    The construction of zipper-like chromophore-arrays in the major groove of duplex DNA remains a challenge because only a few chromophores for this application have been discovered. To address the challenge, dual-chromophore labeled DNAs having a self-complementary sequence were prepared using a solid-phase, post-synthetic, copper-catalyzed, alkyne-azide cycloaddition. The resulting chromophore-arrays on the labeled DNA duplexes were characterized. The dual-tetraphenylethene (TPE) or dual-pyrene (Py) labeled DNA formed self-complementary B-form duplexes and resulted in the construction of chromophore-arrays in the major groove. The TPE-arrays, in which TPEs were arranged in a zipper-like fashion, slightly destabilized the duplex because of their bulkiness and exhibited aggregation-induced-emission. The Py-arrays, in which Pys were not arranged in a zipper-like fashion, had no effect on duplex stability and exhibited weak excimer emission because Py was sufficiently small for free rotation in the major groove.
  • Tadao Takada, Kazue Syunori, Mitsunobu Nakamura, Kazushige Yamana
    Analyst 144(21) 6193-6196 2019年  査読有り
    We here describe a photocurrent generation system exploiting gold nanoparticles (AuNPs) that cover perylenediimide-DNA complexes on electrode surfaces. Enhanced photocurrents were generated by the irradiation of the AuNPs, attributed to the efficient excitation of the perylenediimides by a local electric field on the surface of the AuNPs.

MISC

 10

書籍等出版物

 6

講演・口頭発表等

 6

担当経験のある科目(授業)

 4

共同研究・競争的資金等の研究課題

 12