遊佐 真一

We designed photo-crosslinkable polymer brushes with dimethylmaleimide moieties, in order to demonstrate dynamic stimulation of cell differentiation in mesenchymal stem cells (MSCs). The polymer brushes were synthesized by surface-initiated reversible addition fragmentation chain transfer polymerization using dimethylmaleimide ethyl methacrylate and methyl methacrylate on a chain transfer agent-immobilized glass surface. The polymer brushes were crosslinked by photodimerization of the dimethylmaleimide moieties within polymer chains with stem cells present on the surface. In order to evaluate the effects of in situ photo-induced crosslinking of the polymer brushes on gene expression of stem cells, human bone marrow MSCs were cultured under static and dynamic culture conditions for 7 days. Expression of the osteocalcin (Ocn) gene in MSCs was used as an indicator of osteoblast differentiation under dynamic culture conditions. Structural conversion from non-crosslinked polymer brushes to crosslinked polymer brushes increased the expression of Ocn by 1.4-fold in the presence of adhered cells, compared with non-crosslinked polymer brushes under static culture conditions. These results

気液界面に固体粒子が吸着することにより大気中で安定化した液滴は，リキッドマーブルと呼ばれる。pH，温度，光，磁力等の外部刺激に応答する固体微粒子を安定化剤として利用することで，安定性，構造のコントロールが可能なリキッドマーブルを創出できる。このような外部刺激による内部物質の取り出し，液体のカプセル化が可能なリキッドマーブルは，新しい化学プロセス，機能性材料創出の可能性を拡げるソフトマテリアルになると期待できる。