研究者業績

田中 更沙

タナカ サラサ  (Sarasa Tanaka)

基本情報

所属
兵庫県立大学 環境人間学部 食環境栄養課程 助教
学位
博士(栄養学)(2013年3月)

J-GLOBAL ID
202001018798806567
researchmap会員ID
R000000447

研究キーワード

 1

論文

 23
  • Sarasa Tanaka, Hiromi Kawamura, Yumeno Imoto, Yuri Urata, Sayuka Hontama, Momoko Oda, Motoyoshi Sakaue, Mikiko Ito
    Journal of clinical biochemistry and nutrition 72(2) 126-131 2023年3月  
    Refeeding syndrome is a major clinical problem that leads to fatal complications in patients suffering from malnutrition. Hypophosphatemia inevitably is observed at the onset of refeeding syndrome and therefore is monitored during refeeding; however, the causes of metabolic changes in phosphate concentration during refeeding remain poorly understood. In a previous study, we established a refeeding syndrome model employing total parenteral nutrition with insulin-induced hypophosphatemia, but the symptoms were severe and the metabolic mechanisms in this model may not have been representative of clinical conditions. Therefore, we established a new animal model of mild refeeding syndrome by using a shorter fasting period followed by a single refeeding. These mild refeeding syndrome-model rats exhibited hypophosphatemia without increases in urinary phosphate excretion. Interestingly, administration of the combination of phosphate and insulin during refeeding promoted insulin secretion during refeeding. This model implies that Pi may directly promote insulin secretion in pancreatic cells. These results clarify the interaction between phosphate and glucose metabolism pancreatic cells during refeeding syndrome in a mild refeeding syndrome model.
  • Naoko Suga, Akira Murakami, Hideyuki Arimitsu, Kazuya Shiogama, Sarasa Tanaka, Mikiko Ito, Yoji Kato
    Journal of clinical biochemistry and nutrition 69(1) 61-67 2021年7月  
    Inflammatory bowel diseases, including Crohn's disease and ulcerative colitis, are chronic inflammatory disorders associated with oxidative stress. The intestines produce 5-hydroxytryptamine that may negatively affect disease state under inflammatory conditions when overproduced. 5-Hydroxytryptamine is a substrate for myeloperoxidase and is converted into reactive tryptamine-4,5-dione. Here, an experimental colitis model was established through oral administration of 5% dextran sulfate sodium to ICR mice for 7 days. Furthermore, the formation of tryptamine-4,5-dione in the colorectal mucosa/submucosa and colorectal tissue was analyzed by chemical and immunochemical methodologies. First, free tryptamine-4,5-dione in the homogenate was chemically trapped by o-phenylenediamine and analyzed as the stable phenazine derivative. Tryptamine-4,5-dione localization as adducted proteins in the colorectal tissue was immunohistochemically confirmed, and as demonstrated by both methods, this resulted in the significant increase of tryptamine-4,5-dione in dextran sulfate sodium-challenged mice compared with control mice. Immunohistochemical staining confirmed tryptamine-4,5-dione-positive staining at the myeloperoxidase accumulation site in dextran sulfate sodium-challenged mice colorectal tissue. The tryptamine-4,5-dione locus in the mice was partly matched with that of a specific marker for myeloperoxidase, halogenated tyrosine. Overall, the results possibly indicate that tryptamine-4,5-dione is generated by neutrophil myeloperoxidase in inflammatory tissue and may contribute to the development of inflammatory bowel disease.
  • Mariko Tani, Sarasa Tanaka, Chihiro Oeda, Yuichi Azumi, Hiromi Kawamura, Motoyoshi Sakaue, Mikiko Ito
    Journal of clinical biochemistry and nutrition 68(1) 23-31 2021年1月  
    Vascular calcification is major source of cardiovascular disease in patients with chronic kidney disease. Hyperphosphatemia leads to increased intracellular phosphorus influx, which leads to an increase in osteoblast-like cells in vascular smooth muscle cell. PiT-1 transports phosphate in vascular smooth muscle cell. However, the mechanism of vascular calcification is not completely understood. This study investigated candidate phosphorus-related molecules other than PiT-1. We hypothesized that phosphorus-related molecules belonging to the solute-carrier (SLC) superfamily would be involved in vascular calcification. As a result of DNA microarray analysis, we focused on SLC37A2 and showed that mRNA expression of these cells increased on calcified aotic smooth muscle cells (AoSMC). SLC37A2 has been reported to transport both glucose-6-phosphate/phosphate and phosphate/phosphate exchanges. In vitro analysis showed that SLC37A2 expression was not affected by inflammation on AoSMC. The expression of SLC37A2 mRNA and protein increased in calcified AoSMC. In vivo analysis showed that SLC37A2 mRNA expression in the aorta of chronic kidney disease rats was correlated with osteogenic marker genes. Furthermore, SLC37A2 was expressed at the vascular calcification area in chronic kidney disease rats. As a result, we showed that SLC37A2 is one of the molecules that increase with vascular calcification in vitro and in vivo.
  • Mariko Tani, Sarasa Tanaka, Kana Takamiya, Yoji Kato, Gaku Harata, Fang He, Motoyoshi Sakaue, Mikiko Ito
    Journal of clinical biochemistry and nutrition 67(3) 283-289 2020年11月  査読有り
    Vascular calcification progresses under hyperphosphatemia, and represents a risk factor for cardiovascular disease in chronic kidney disease (CKD) patients. We recently indicated that phosphorus (P) fluctuations also exacerbated vascular calcification in early-stage CKD rats. Dietary fiber intake is reportedly associated with cardiovascular risk. This study investigated the effects of dietary fiber on vascular calcification by repeated P fluctuations in early-stage CKD rats. Unilateral nephrectomy rats were used as an early-stage CKD model. For 36 days, a P fluctuation (LH) group was fed low-P (0.02% P) and high-P (1.2% P) diets alternating every 2 days, and a P fluctuation with dietary fiber intake (LH + F) group was fed low-P and high-P diets containing dietary fiber alternating every 2 days. The effect on vascular calcification was measured calcium content. Effects on uremic toxin were measured levels of indoxyl sulfate (IS) and investigated gut microbiota. The LH + F group showed significantly reduced vessel calcium content compared to the LH group. Further, dietary fiber inhibited increases in blood levels of IS after intake of high-P diet, and decreased uremic toxin-producing intestinal bacteria. Dietary fiber may help suppress progression of vascular calcification due to repeated P fluctuations in early-stage CKD rats by decreasing uremic toxin-producing intestinal bacteria.
  • Keisuke Kawamoto, Masae Sakuma, Sarasa Tanaka, Masashi Masuda, Mari Nakao-Muraoka, Yuki Niida, Yurino Nakamatsu, Mikiko Ito, Yutaka Taketani, Hidekazu Arai
    Nutrition (Burbank, Los Angeles County, Calif.) 72 110694-110694 2020年4月  査読有り
    OBJECTIVE: The ratio of dietary carbohydrate to fat may affect phosphorus metabolism because both calcium and phosphorus are regulated by similar metabolic mechanisms, and a high-fat diet (HFD) induces deleterious effects on the absorption of dietary calcium. We hypothesized that an HFD induces an increase in phosphorus absorption. The aim of this study was to evaluate the effects of differences in the quantity and quality of dietary fat on phosphorus metabolism over the short- and long-term. METHODS: Eighteen 8-wk-old Sprague-Dawley male rats were fed an isocaloric diet containing varied ratios of carbohydrates to fat energy and sources of fat (control diet, HFD, and high- saturated fat diet [HF-SFA]). At 3 d and 7 wk after the allocation and initiation of the test diets, feces and urine were collected and used for phosphorus and calcium measurement. RESULTS: The fecal phosphorous concentration (F-Pi) was lower in the HF-SFA group than in the other two groups; however, the urine phosphorus concentration (U-Pi) was significantly higher in the HF-SFA group than the other two groups when the rats were fed over the short- (P < 0.01) and long -term (P < 0.01 versus control, P < 0.05 versus HFD group). There were no significant differences in type-IIa sodium-phosphate cotransporter (NaPi-2 a) and type-IIc sodium-phosphate cotransporter (NaPi-2 c) mRNA expression, which are renal phosphate transport-related genes; however, the expression of type-IIb sodium-phosphate cotransporter (NaPi-2 b) and type-III sodium-phosphate cotransporter (Pit-1) mRNA in the duodenum was higher in the HFD and HF-SFA groups than in the control group (P < 0.05), although there were no significant differences in these in the jejunum. CONCLUSIONS: The present results indicated that an HFD, particularly HF-SFA, increases intestinal phosphate absorption compared with control.

MISC

 8
  • 山本 浩範, 石黒 真理子, 福田 詩織, 中橋 乙起, 田中 更沙, 増田 真志, 武田 英二, 竹谷 豊
    四国医学雑誌 79(5-6) 209-214 2024年3月  
  • 金子一郎, 田中更沙
    Phosphorus Letter (108) 2023年  
  • 山本 浩範, 石黒 真理子, 田中 更沙, 竹井 悠一郎, 増田 真志, 大西 康太, 竹谷 豊
    ビタミン 94(4) 246-246 2020年4月  
  • 伊藤 美紀子, 田中 更沙
    Clinical Calcium 26(3) 375-383 2016年2月  
    骨粗鬆症をはじめとした骨疾患では、栄養や食習慣と深い関連があり、その予防ならびに改善において栄養を抜きにして考えることはできない。我が国は2007年に超高齢社会に突入しており、さらなる骨粗鬆症患者の増加が懸念されている。骨粗鬆症と栄養、特にカルシウムとの関係は一般の認知度が高いにもかかわらず、栄養摂取の目標達成には全く至っていない。また、過去の病気と思われていた小児のビタミンD欠乏性くる病が、妊産婦のビタミンD欠乏、母乳栄養の推奨、乳児期の日光への曝露不足などから増加傾向にある。骨疾患予防のためには若年期からの生活習慣・食生活の改善が重要である。(著者抄録)
  • 田中 更沙, 伊藤 美紀子
    Clinical Calcium 25(7) 1057-1062 2015年6月  
    腎性骨症は慢性腎臓病(CKD)またはその治療に伴い起こる骨組織障害の総称であり、腎機能の低下に伴うミネラル代謝異常により骨代謝に異常をきたす病態である。ミネラル代謝異常改善のため、P、Caの管理が重要であることが明らかとなっている。CKD患者における特徴的な栄養障害としてprotein-energy wasting(PEW)が知られており、生命予後と強く関連する。このようにCKD患者において食事療法は治療において大きな役割を占めており、腎性骨症においてもP、Caの管理および全身の栄養状態の改善に栄養管理は重要であると考えられる。(著者抄録)

講演・口頭発表等

 35

共同研究・競争的資金等の研究課題

 8