CVClient

西野 有里

ニシノ ユリ  (Yuri Nishino)

基本情報

所属
兵庫県立大学 大学院 生命理学研究科 助教
学位
薬学修士(大阪大学大学院薬学研究科)
博士(理学)(兵庫県立大学大学院生命理学研究科)

J-GLOBAL ID
200901096220712031
researchmap会員ID
5000072128

論文

 49
  • Atsushi Ohma, Kazuki Arihara, Tetsuya Mashio, Yoshiko Ito, Yuri Nishino, Atsuo Miyazawa
    International Journal of Electrochemical Science 19(5) 100539-100539 2024年5月  査読有り
  • Masamitsu Wada, Takeshi Higa, Kaoru Katoh, Nobuko Moritoki, Tomonori Nakai, Yuri Nishino, Atsuo Miyazawa, Shinsuke Shibata, Yoshinobu Mineyuki
    Journal of plant research 2024年4月10日  査読有り
    Chloroplast-actin (cp-actin) filaments are crucial for light-induced chloroplast movement, and appear in the front region of moving chloroplasts when visualized using GFP-mouse Talin. They are short and thick, exist between a chloroplast and the plasma membrane, and move actively and rapidly compared to cytoplasmic long actin filaments that run through a cell. The average period during which a cp-actin filament was observed at the same position was less than 0.5 s. The average lengths of the cp-actin filaments calculated from those at the front region of the moving chloroplast and those around the chloroplast periphery after stopping the movement were almost the same, approximately 0.8 µm. Each cp-actin filament is shown as a dotted line consisting of 4-5 dots. The vector sum of cp-actin filaments in a moving chloroplast is parallel to the moving direction of the chloroplast, suggesting that the direction of chloroplast movement is regulated by the vector sum of cp-actin filaments. However, once the chloroplasts stopped moving, the vector sum of the cp-actin filaments around the chloroplast periphery was close to zero, indicating that the direction of movement was undecided. To determine the precise structure of cp-actin filaments under electron microscopy, Arabidopsis leaves and fern Adiantum capillus-veneris gametophytes were frozen using a high-pressure freezer, and observed under electron microscopy. However, no bundled microfilaments were found, suggesting that the cp-actin filaments were unstable even under high-pressure freezing.
  • Koichiro Oishi, Mayu Nagamori, Yasuhiro Kashino, Hiroshi Sekiguchi, Yuji C. Sasaki, Atsuo Miyazawa, Yuri Nishino
    International Journal of Molecular Sciences 24(15) 2023年7月28日  査読有り最終著者責任著者
    Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that play an important role in signal transduction at the neuromuscular junction (NMJ). Movement of the nAChR extracellular domain following agonist binding induces conformational changes in the extracellular domain, which in turn affects the transmembrane domain and opens the ion channel. It is known that the surrounding environment, such as the presence of specific lipids and proteins, affects nAChR function. Diffracted X-ray tracking (DXT) facilitates measurement of the intermolecular motions of receptors on the cell membranes of living cells, including all the components involved in receptor function. In this study, the intramolecular motion of the extracellular domain of native nAChR proteins in living myotube cells was analyzed using DXT for the first time. We revealed that the motion of the extracellular domain in the presence of an agonist (e.g., carbamylcholine, CCh) was restricted by an antagonist (i.e., alpha-bungarotoxin, BGT).
  • 西野有里, 伊藤喜子, 宮澤淳夫
    顕微鏡 57(3) 139-144 2022年12月  招待有り筆頭著者
  • Junichi Shimanuki, Hideto Imai, Yoshiko Ito, Yuri Nishino, Atsuo Miyazawa
    Microscopy (Oxford, England) 2022年11月17日  査読有り
    It is important to understand and control the fine structure of the fuel cell catalyst layer in order to improve the battery characteristics of the fuel cell. A major challenge in observing the microstructure of the catalyst layer by electron microscopy is the visualization of ionomers, which have low contrast and are susceptible to damage by electron beam irradiation. Previous papers have reported transmission electron microscopy (TEM) observations of ionomers neutralized with cesium (Cs) ions. However, this approach involves chemical reactions and an indirect visualization of ionomers. In contrast, we have previously revealed the microstructure of ionomers in frozen catalyst inks by cryogenic scanning electron microscopy (cryo-SEM) and cryo-TEM. In general, ionomers are basically used under high temperature and humid conditions while the fuel cell is operating. Therefore, in this study, ultrathin sections prepared from the fuel cell catalyst layer (membrane electrode assemblies) were incubated in the chamber at high temperature and humid conditions and then rapidly frozen for observation by cryo-TEM. As a result, we succeeded in observing the pore structure of the catalyst layer in the swollen state of the ionomer. The swollen ionomer surrounded and enclosed the Pt/C aggregates and bridged over the pores in the catalyst layer.

MISC

 31

書籍等出版物

 1

講演・口頭発表等

 15

共同研究・競争的資金等の研究課題

 7

産業財産権

 1

学術貢献活動

 2