Legal Medicine

isobe ichiro

  (磯部 一郎)

Profile Information

Affiliation
Professor, School of Medicine Faculty of Medicine, Fujita Health University
Degree
博士(医学)(名古屋市立大学)

J-GLOBAL ID
200901028150391342
researchmap Member ID
5000029856

Misc.

 6
  • 中留真人, 平田ゆかり, 濱島 誠, 磯部一郎
    DNA多型, 21 270-273, 2013  
  • 中留真人, 折井みなみ, 濱島誠, 平田ゆかり, 日比淑江, 布谷美弥, 磯部一郎
    DNA多型, 20 298-302, 2012  
  • Masato Nakatome, Minami Orii, Makoto Hamajima, Yukari Hirata, Misato Uemura, Sayaka Hirayama, Ichiro Isobe
    LEGAL MEDICINE, 13(4) 205-209, Jul, 2011  
    DNA methylation in gene promoter regions influences gene expression. Circadian clock genes play an important role in the formation of a biological clock and aberrant methylation of these genes contributes to several disorders. In this study, we examined forensic autopsy specimens to determine whether DNA methylation status in the promoter regions of nine circadian clock genes (Per1, Per2, Per3, Cry1, Cry2, Bmal1, Clock, Tim, and Ck1e) is related to a change in acquired diathesis and/or causes of death. Methylation-specific PCR and direct sequencing methods revealed that the promoters of Per1, Cry2, Bmal1, Clock, and Ck1e were unmethylated in all the forensic autopsy specimens, while the promoters of Per2, Per3, Cry1, and Tim were partially methylated. Methylation status varied between individuals and between tissues in the same patient. A detailed analysis of methylation patterns in the Cry1 promoter region revealed that the patterns also varied between individuals and the Cry1 promoter had highly methylated patterns in two cases that had been exposed to methamphetamine. These results suggest that the methylation status of clock gene promoters varies between individuals. Methamphetamine use may influence methylation in the Cry1 gene promoter region and disturb circadian rhythmicity. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
  • 中留真人, 折井みなみ, 濱島誠, 平田ゆかり, 植村美里, 平山沙也香, 磯部一郎
    DNA多型, 19 240-244, 2011  
  • Masato Nakatome, Takuma Yamamoto, Ichiro Isobe, Ryoji Matoba
    Legal Medicine, 11(6) 298-301, Nov, 2009  
    Inherited mutations in the human cardiac sodium channel (SCN5A) gene cause arrhythmogenic diseases such as tachyarrhythmia and bradyarrhythmia. Moreover, mutation subsets in the coding region impair SCN5A function, potentially leading to sudden cardiac death (SCD). In the present study, we performed diplotype analysis of the regulatory region of the SCN5A gene in Japanese people who died suddenly because of an unknown cause (sudden death group n = 70) and controls (n = 112). There were no significant differences at six polymorphic loci between the groups. However, 38 diplotypes of 6-nucleotide polymorphism variants were identified. One of these diplotypes-Dip.D (CTG-TC/CCG-TC)-occurred significantly more frequently in the sudden death group than in the controls (p &lt 0.01, OR = 5.18, 95% CI: 1.38-19.45). Dip.D has two variants (T-1062C and T-847G), and while it is unclear whether these directly affect mRNA expression, a common polymorphism in this region modulates SCN5A expression in vitro. Our results thus suggest that the transcription of the SCN5A Dip.D variant may be associated with arrhythmogenic diseases that can induce sudden death. © 2009 Elsevier Ireland Ltd. All rights reserved.

Books and Other Publications

 1

Presentations

 17

Professional Memberships

 1

Research Projects

 4

Other

 1

教育内容・方法の工夫(授業評価等を含む)

 1
  • 件名(英語)
    授業毎にプリントの配布・画像資料の提示を行い、基礎事項の定着を図る。
    開始年月日(英語)
    2009
    終了年月日(英語)
    2013
    概要(英語)
    指定教科書の重要な医用を中心にまとめたプリントを作成し重要な知識の離開を図るとともに、法医学に特徴的な画像情報を精選してスライドで提示し、具体的な所見のイメージを把握させた。

作成した教科書、教材、参考書

 1
  • 件名(英語)
    NEWエッセンシャル法医学第5版
    終了年月日(英語)
    2012
    概要(英語)
    損傷 外傷性ショック・ショックの病態生理、その他の損傷.高取健彦監修,長尾正崇他編.NEWエッセンシャル法医学第5版.東京:医歯薬出版;2012;p143-51. を分担執筆。

その他教育活動上特記すべき事項

 3
  • 件名(英語)
    第23、33、42回医学教育ワークショップ
    開始年月日(英語)
    2010
    終了年月日(英語)
    2012
    概要(英語)
    「CBT試験問題作成」に参加。
  • 件名(英語)
    学生指導委員
    開始年月日(英語)
    2008
    終了年月日(英語)
    2012
    概要(英語)
    M4学年担任
  • 件名(英語)
    学生指導委員長
    開始年月日(英語)
    2013