General Education

松井 太衛

マツイ タエイ  (Matsui Taei)

基本情報

所属
藤田医科大学 医療科学部 臨床検査学科 自然科学 生物学 教授 (特任教授)
奈良県立医科大学輸血部 非常勤講師
学位
博士(理学)

J-GLOBAL ID
200901001023876480
researchmap会員ID
1000102760

外部リンク

学歴

 2

委員歴

 2

論文

 120
  • Atsushi Ogasawara, Hiroki Doi, Taei Matsui, Etsuko Tokunaga, Masao Amakawa, Hidehiko Akiyama
    Fujita medical journal 9(2) 147-153 2023年5月  
    OBJECTIVES: Agaritine (AGT) is a hydrazine-containing compound derived from the mushroom Agaricus blazei Murill. We previously reported the antitumor effect of AGT on hematological tumor cell lines and suggested that AGT induces apoptosis in U937 cells via caspase activation. However, the antitumor mechanism of AGT has not been fully understood. METHODS: Four hematological tumor cell lines (K562, HL60, THP-1, H929) were used in this study. The cells were incubated in the presence of 50 μM AGT for 24 h and analyzed for cell viability, annexin V positivity, caspase-3/7 activity, mitochondrial membrane depolarization, cell cycle, DNA fragmentation, and the expression of mitochondrial membrane-associated proteins (Bax and cytochrome c). RESULTS: In HL60, K562, and H929 cells, AGT reduced cell viability and increased annexin V- and dead cell-positive rates; however, it did not affect THP-1 cells. In K562 and HL60 cells, caspase-3/7 activity, mitochondrial membrane depolarization, and expression of mitochondrial membrane proteins, Bax and cytochrome c, were all increased by AGT. Cell cycle analysis showed that only K562 exhibited an increase in the proportion of cells in G2/M phase after the addition of AGT. DNA fragmentation was also observed after the addition of AGT. CONCLUSIONS: These results indicate that AGT induces apoptosis in K562 and HL60 cells, like U937 reported previously, but showed no effect on THP-1 cells. It was suggested that AGT-induced apoptosis involves the expression of Bax and cytochrome c via mitochondrial membrane depolarization.
  • 早川 正樹, 大前 和人, 田中 宏明, 谷山 歩, 田邊 雅世, 馬塲 由美, 下村 志帆, 梅木 弥生, 長谷川 真弓, 松井 太衛, 松本 雅則
    日本輸血細胞治療学会誌 68(2) 311-311 2022年4月  
  • Youhei Nashimoto, Fumio Matsushita, Johannes M Dijkstra, Yuta Nakamura, Hidehiko Akiyama, Jiharu Hamako, Takashi Morita, Satohiko Araki, Taei Matsui
    Toxins 14(4) 2022年3月25日  
    Bitiscetin-1 (aka bitiscetin) and bitiscetin-2 are C-type lectin-like proteins purified from the venom of Bitis arietans (puff adder). They bind to von Willebrand factor (VWF) and-at least bitiscetin-1-induce platelet agglutination via enhancement of VWF binding to platelet glycoprotein Ib (GPIb). Bitiscetin-1 and -2 bind the VWF A1 and A3 domains, respectively. The A3 domain includes the major site of VWF for binding collagen, explaining why bitiscetin-2 blocks VWF-to-collagen binding. In the present study, sequences for a novel bitiscetin protein-bitiscetin-3-were identified in cDNA constructed from the B. arietans venom gland. The deduced amino acid sequences of bitiscetin-3 subunits α and β share 79 and 80% identity with those of bitiscetin-1, respectively. Expression vectors for bitiscetin-3α and -3β were co-transfected to 293T cells, producing the heterodimer protein recombinant bitiscetin-3 (rBit-3). Functionally, purified rBit-3 (1) induced platelet agglutination involving VWF and GPIb, (2) did not compete with bitiscetin-1 for binding to VWF, (3) blocked VWF-to-collagen binding, and (4) lost its platelet agglutination inducing ability in the presence of an anti-VWF monoclonal antibody that blocked VWF-to-collagen binding. These combined results suggest that bitiscetin-3 binds to the A3 domain, as does bitiscetin-2. Except for a small N-terminal fragment of a single subunit-which differs from that of both bitiscetin-3 subunits-the sequences of bitiscetin-2 have never been determined. Therefore, by identifying and analyzing bitiscetin-3, the present study is the first to present the full-length α- and β-subunit sequences and recombinant expression of a bitiscetin-family toxin that blocks the binding of VWF to collagen.
  • 日笠 聡, 渥美 達也, 石黒 精, 金子 誠, 高橋 芳右, 野上 恵嗣, 藤井 輝久, 堀内 久徳, 松井 太衛, 毛利 博, 森下 英理子, 松下 正, 朝比奈 俊彦, 天野 景裕, 上田 恭典, 岡本 好司, 小亀 浩市, 佐道 俊幸, 瀧 正志, 長尾 梓, 西尾 健治, 西田 恭治, 西野 正人, 藤村 吉博, 松本 雅則, 宮川 義隆, 八木 秀男, 和田 英夫, von Willebrand病の診療ガイドライン作成委員会
    日本血栓止血学会誌 32(4) 413-481 2021年8月  
  • 日笠 聡, 渥美達也, 石黒 精, 金子 誠, 高橋芳右, 野上恵嗣, 藤井輝久, 堀内久徳, 松井太衛, 毛利博, 森下英理子, 松下 正
    日本血栓止血学会誌 32(4) 413-481 2021年8月  査読有り招待有り

MISC

 53

書籍等出版物

 12

講演・口頭発表等

 68

担当経験のある科目(授業)

 11

共同研究・競争的資金等の研究課題

 20

教育内容・方法の工夫(授業評価等を含む)

 1
  • 件名
    -
    開始年月日
    2010
    概要
    相互研修FD出席

作成した教科書、教材、参考書

 2
  • 件名
    基礎科学実験(生物学)テキスト
  • 件名
    自然科学情報論演習テキスト

教育方法・教育実践に関する発表、講演等

 1
  • 件名
    -
    終了年月日
    2009/08
    概要
    第2回相互研修FDで発表

その他教育活動上特記すべき事項

 2
  • 件名
    -
    開始年月日
    2010/04
    終了年月日
    2012/03
    概要
    医療科学部学生指導委員会副委員長
  • 件名
    -
    開始年月日
    2013/04
    概要
    医療科学部学生指導委員会副委員長