Makoto Hibiya

軽度低体温体外循環と中等度低体温体外循環におけるサイトカインの血中濃度変化を比較的検討した.IL6は軽度低体温で有意に増加したがIL1β,IL8では差は認めなかった

【要旨】体外循環を用いた開心術症例群と肺切除術症例群における,血中サイトカイン誘導の比較検討を行った。対象は体外循環群10例,肺切除群9例である。IL-1β,IL-6,IL-10およびTGF-β1の術前および術直後の血中濃度を測定した。体外循環例ではIL-1β,IL-6,IL-10およびTGF-β1ともに有意に増加したが,肺切除例ではIL-1βおよびTGF-β1は増加しなかった。IL-6,IL-10の増加は体液性免疫能に関与しており,両群ともに体液性免疫機構の発現が示唆された。しかし,抑制性サイトカインであり,細胞性免疫能に関与するTGF-β1の増加は肺切除例ではみられなかった。これらのことから,体外循環群は体液性免疫機構のみならず,細胞性免疫機構をも活性化することが示唆され,体外循環群の生体におよぼす非生理的侵襲の強さが示された。

We previously reported a HPLC assay method using fluorimetric detection for the simultaneous determination of urinary N-2-(3-aminopropyl)biopterin (oncopterin, a natural pteridine newly found in urine from cancer patients), biopterin and neopterin. We now have observed that an unknown substance, which may be derived from methotrexate, in urine from a patient with stomach cancer interfered with the assay of oncopterin and demonstrated that oncopterin could be completely separated from the unidentified substance by HPLC using a Nucleosil 100-5SA strong cation-exchange column. Furthermore, oncopterin was not detectable by this HPLC-fluorimetric method in urine samples from patients with stomach cancer who were not treated with methotrexate. The content of urinary oncopterin from cancer patients is supposed to be very low, with less than 1 mu mol/mol creatinine. The present results indicate that the peak found with elution from the C-18 column was a methotrexate-derived compound and co-eluted with the analyte oncopterin.

A high-performance liquid chromatographic method is described for the simultaneous determination of N-2-(3-aminopropyl)biopterin (oncopterin, a newly found natural pteridine in urine from cancer patients), biopterin, and neopterin in urine. For the detection and quantification of the compounds, fluorometry was used. Using Develosil ODS K-5 and Develosil ODS HG-5 reversed-phase columns and a Nucleosil 100-5SA strong cation-exchange column, oncopterin, biopterin, and neopterin in urine were completely separated and assayed simultaneously by fluorescence detection. Similar values of oncopterin were obtained using each of the three columns, and the Develosil ODS K-5 reversed-phase column gave the most satisfactory separation. The sensitivity was high enough to measure 1 pmol of each pteridine. The HPLC method was highly reproducible. Our preliminary results indicate that oncopterin could be a most sensitive marker for cancer.

Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the μ-δ intron, two repeating units were contained. One 442-base-long fragment located JH-μ intron ( defined as "sigma μ(σμ)") occupied the position in the μ-σ intron. The other 1166-base-long fragment located somewhere in front of S (class switch) region of Cγ gene was also found in the μ-δ intron. We defined the repeats in the μ-δ intron as "SIGMA (Σ)". The polarities of the longer repeats in the genome were opposite between the μ-δ intron and the upstreams of Cγ genes. These inverted copies (defined as σγ3 and σγ4), located 6 kb upstream of their respective Cγ's, were apparently transcribed in vitro, via RNA polymerase III and transcripts should have contained tRNA-like structures. Small DNA fragments capable of encoding tRNA-like structures were also found in corresponding regions of mouse Ig Cγ cluster. © 1988 IRL Press Limited, Oxford, England.