医療科学部

Hiroyuki Tezuka

  (手塚 裕之)

Profile Information

Affiliation
Research Promotion Headquarters, Fujita Health University
Degree
博士(医学)(東京医科歯科大学)

Researcher number
30375258
J-GLOBAL ID
201601006012155800
researchmap Member ID
B000251084

External link

Major Papers

 11
  • Hiroyuki Tezuka, Yukiko Abe, Jumpei Asano, Taku Sato, Jiajia Liu, Makoto Iwata, Toshiaki Ohteki
    Immunity, 34(2) 247-257, Feb, 2011  Peer-reviewedLead author
    Although both conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) are present in the gut-associated lymphoid tissues (GALT), the roles of pDCs in the gut remain largely unknown. Here we show a critical role for pDCs in T cell-independent (TI) IgA production by B cells in the GALT. When pDCs of the mesenteric lymph nodes (MLNs) and Peyer's patches (PPs) (which are representative GALT) were cultured with naive B cells to induce TI IgA class switch recombination (CSR), IgA production was substantially higher than in cocultures of these cells with cDCs. IgA production was dependent on APRIL and BAFF production by pDCs. Importantly, pDC expression of APRIL and BAFF was dependent on stromal cell-derived type I IFN signaling under steady-state conditions. Our findings provide insight into the molecular basis of pDC conditioning to induce mucosal TI IgA production, which may lead to improvements in vaccination strategies and treatment for mucosal-related disorders.
  • Hiroyuki Tezuka, Yukiko Abe, Makoto Iwata, Hajime Takeuchi, Hiromichi Ishikawa, Masayuki Matsushita, Tetsuo Shiohara, Shizuo Akira, Toshiaki Ohteki
    Nature, 448(7156) 929-933, Aug, 2007  Peer-reviewedLead author
    Immunoglobulin-A has an irreplaceable role in the mucosal defence against infectious microbes(1-6). In human and mouse, IgA-producing plasma cells comprise similar to 20% of total plasma cells of peripheral lymphoid tissues, whereas more than 80% of plasma cells produce IgA in mucosa-associated lymphoid tissues (MALT)(1-6). One of the most biologically important and long-standing questions in immunology is why this 'biased' IgA synthesis takes place in the MALT but not other lymphoid organs. Here we show that IgA class-switch recombination (CSR) is impaired in inducible-nitric-oxide-synthase-deficient (iNOS(-/-); gene also called Nos2) mice. iNOS regulates the T-cell-dependent IgA CSR through expression of transforming growth factor-beta receptor, and the T-cell-independent IgA CSR through production of a proliferation-inducing ligand (APRIL, also called Tnfsf13) and a B-cell-activating factor of the tumour necrosis factor (TNF) family (BAFF, also called Tnfsf13b). Notably, iNOS is preferentially expressed in MALT dendritic cells in response to the recognition of commensal bacteria by toll-like receptor. Furthermore, adoptive transfer of iNOS(+) dendritic cells rescues IgA production in iNOS(-/-) mice. Further analysis revealed that the MALT dendritic cells are a TNF-alpha/iNOS-producing dendritic-cell subset, originally identified in mice infected with Listeria monocytogenes(7,8). The presence of a naturally occurring TNF-alpha/iNOS-producing dendritic-cell subset may explain the predominance of IgA production in the MALT, critical for gut homeostasis.

Misc.

 18

Books and Other Publications

 4

Presentations

 9

Major Teaching Experience

 17

Research Projects

 7

Academic Activities

 2