研究者業績

熊野 恵城

クマノ ケイキ  (KUMANO KEIKI)

基本情報

所属
武蔵野大学 薬学部 教授
学位
博士(医学)(1999年3月 東京大学)

J-GLOBAL ID
201801003635316249
researchmap会員ID
B000327851

学歴

 2

論文

 104
  • Kazuhiko Ishigaki, Keiki Kumano, Kyohei Fujita, Hiroo Ueno
    Scientific Reports 11(1) 2021年12月  
    <title>Abstract</title>Although the physiological function of the omentum remains elusive, it has been proposed that it plays an important role in fat storage, immune regulation, and regeneration of injured tissues and organs. Although the omentum undergoes expansion upon activation, reports on the accurate assessment of increased cell types and the origin of the increased cells remain limited. To investigate this aspect, the omenta of parabiotic mice were subjected to activation using distinct fluorescent markers and single-cell RNA sequencing (scRNA-seq) was performed to identify circulation-derived omental cells. We found that a considerable number of circulating cells contributed to the activation of the omentum. The omental cells derived from circulating cells exhibited morphological features similar to those of fibroblasts. scRNA-seq revealed the existence of a novel cell population that co-expressed macrophage and fibroblast markers in the activated omentum, suggesting that it corresponded to circulating macrophage-derived fibroblast-like cells. Lineage tracing experiments revealed that most fibroblasts in the activated omentum were not derived from WT1-positive mesenchymal progenitors. The cell cluster also expressed various chemokine genes, indicating its role in the activation and recruitment of immune cells. These results provide important information regarding the activation of the omentum.
  • Matsukawa, T, Yamamoto, T, Honda, A, Toya, T, Ishiura, H, Mitsui, J, Tanaka, M, Hao, A, Shinohara, A, Ogura, M, Kataoka, K, Seo, S, Kumano, K, Hosoi, M, Narukawa, N, Yasunaga, A, Maki, H, Ichikawa, M, Nannya, Y, Imai, Y, Takahashi, T, Takahashi, Y, Nagasako, Y, Yasaka, K, Koshi Mano K, Kawabe Matsukawa, M, Miyagawa, T, Hamada, M, Sakuishi, K, Hayashi, T, Iwata, A, Terao, Y, Shimizu, J, Goto, J, Mori, M, Kunimatsu, A, Aoki, S, Hayashi, S, Nakamura, F, Arai, S, Monma, K, Ogata, K, Yoshida, T, Abe, O, Inazawa, J, Toda, T, Kurokawa, M, Tsuji, S
    Brain Communications 2(1) fcz048 2020年1月  査読有り
  • Taoka K, Arai S, Kataoka K, Hosoi M, Miyauchi M, Yamazaki S, Honda A, Aixinjueluo W, Kobayashi T, Kumano K, Yoshimi A, Otsu M, Niwa A, Nakahata T, Nakauchi H, Kurokawa M
    Scientific reports 8(1) 15855-15855 2018年10月26日  査読有り
  • Masashi Miyauchi, Junji Koya, Shunya Arai, Sho Yamazaki, Akira Honda, Keisuke Kataoka, Akihide Yoshimi, Kazuki Taoka, Keiki Kumano, Mineo Kurokawa
    Stem cell reports 10(3) 1115-1130 2018年3月13日  査読有り
    Properties of cancer stem cells involved in drug resistance and relapse have significant effects on clinical outcome. Although tyrosine kinase inhibitors (TKIs) have dramatically improved survival of patients with chronic myeloid leukemia (CML), TKIs have not fully cured CML due to TKI-resistant CML stem cells. Moreover, relapse after discontinuation of TKIs has not been predicted in CML patients with the best TKI response. In our study, a model of CML stem cells derived from CML induced pluripotent stem cells identified ADAM8 as an antigen of TKI-resistant CML cells. The inhibition of expression or metalloproteinase activity of ADAM8 restored TKI sensitivity in primary samples. In addition, residual CML cells in patients with optimal TKI response were concentrated in the ADAM8+ population. Our study demonstrates that ADAM8 is a marker of residual CML cells even in patients with optimal TKI response and would be a predictor of relapse and a therapeutic target of TKI-resistant CML cells.
  • Hirotsugu Yanai, Naho Atsumi, Toshihiro Tanaka, Naohiro Nakamura, Yoshihiro Komai, Taichi Omachi, Kiyomichi Tanaka, Kazuhiko Ishigaki, Kazuho Saiga, Haruyuki Ohsugi, Yoko Tokuyama, Yuki Imahashi, Shuichi Ohe, Hiroko Hisha, Naoko Yoshida, Keiki Kumano, Masanori Kon, Hiroo Ueno
    SCIENTIFIC REPORTS 7(1) 9891 2017年8月  査読有り
    The murine intestine, like that of other mammalians, continues to develop after birth until weaning; however, whether this occurs in response to an intrinsic developmental program or food intake remains unclear. Here, we report a novel system for the allotransplantation of small intestine and colon harvested from Lgr5(EGFP-IRES-CreERT2/+); Ros alpha 26(rbw/+) mice immediately after birth into the subrenal capsule of wild-type mice. By histological and immunohistochemical analysis, the developmental process of transplanted small intestine and colon was shown to be comparable with that of the native tissues: mature intestines equipped with all cell types were formed, indicating that these organs do not require food intake for development. The intestinal stem cells in transplanted tissues were shown to self-renew and produce progeny, resulting in the descendants of the stem cells occupying the crypt-villus unit of the small intestine or the whole crypt of the colon. Collectively, these findings indicate that neonatal intestine development follows an intrinsic program even in the absence of food stimuli.

MISC

 45
  • 並河 健, 菅野 渉平, 齋賀 一歩, 石垣 和彦, 田中 聖道, 鷲尾 隆太, 山内 壮作, 熊野 恵城, 上野 博夫
    診断病理 35(1) 34-40 2018年1月  
    6ヵ月20日の男児。在胎34週5日、体重1,726gで出生した。Down症候群・TAMと診断され、Ara-Cによる加療を受けたが、日齢185日に呼吸状態が悪化し死亡した。末梢血には日齢1〜15日に芽球が出現し、一旦は消失するが、剖検時の骨髄では20%以上の芽球を認めた。それらはMPO(+)かつCD61(-)で、FAB分類ではDown症候群に典型的なM7ではなくM2を示唆するものであった。肺高血圧症と複合要因により呼吸不全に至った症例であった。その経過について考察を交えて報告する。(著者抄録)
  • Takashi Matsukawa, Tomotaka Yamamoto, Takashi Toya, Akihito Shinohara, Yasuhito Nanya, Sachiko Seo, Keiki Kumano, Motoshi Ichikawa, Yuji Takahashi, Hiroyuki Ishiura, Jun Mitsui, Masaki Tanaka, Mineo Kurokawa, Shoji Tsuji
    ANNALS OF NEUROLOGY 80 S201-S201 2016年10月  
  • Shuichi Ohe, Toshihiro Tanaka, Hirotsugu Yanai, Yoshihiro Komai, Taichi Omachi, Shohei Kanno, Kiyomichi Tanaka, Kazuhiko Ishigaki, Kazuho Saiga, Naohiro Nakamura, Haruyuki Ohsugi, Yoko Tokuyama, Naho Atsumi, Hiroko Hisha, Naoko Yoshida, Keiki Kumano, Fumikazu Yamazaki, Hiroyuki Okamoto, Hiroo Ueno
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 472(1) 292-292 2016年3月  
  • 田中 敏宏, 駒井 資弘, 徳山 陽子, 矢内 洋次, 大江 秀一, 大町 太一, 田中 聖道, 石垣 和彦, 金 桂花, 厚海 奈穂, 菅野 渉平, 吉田 真子, 比舎 弘子, 熊野 恵城, 上野 博夫
    Cytometry Research 24(Suppl.) 58-58 2014年6月  
  • 比舎 弘子, 田中 敏宏, 菅野 渉平, 徳山 陽子, 駒井 資弘, 大江 秀一, 矢内 洋次, 大町 太一, 石垣 和彦, 田中 聖道, 厚海 奈穂, 吉田 直子, 熊野 恵城, 上野 博夫
    日本病理学会会誌 103(1) 274-274 2014年3月  

書籍等出版物

 2

主要な講演・口頭発表等

 30

担当経験のある科目(授業)

 20

共同研究・競争的資金等の研究課題

 22