CVClient

Nomura Nobuo

  (野村 信夫)

Profile Information

Affiliation
Faculty of Human Sciences Department of Human Sciences, Musashino University
Degree
博士(京都大学大学院理学研究科)

Researcher number
20147862
J-GLOBAL ID
201701013411747110
researchmap Member ID
B000269551

Education

 2

Papers

 143
  • Koji Ichiyama, Sindhoora Bhargavi Gopala Reddy, Li Feng Zhang, Wei Xin Chin, Tegshi Muschin, Lars Heinig, Youichi Suzuki, Haraprasad Nanjundappa, Yoshiyuki Yoshinaka, Akihide Ryo, Nobuo Nomura, Eng Eong Ooi, Subhash G Vasudevan, Takashi Yoshida, Naoki Yamamoto
    PLoS neglected tropical diseases, 7(4) e2188, 2013  Peer-reviewed
    Curdlan sulfate (CRDS), a sulfated 1→3-β-D glucan, previously shown to be a potent HIV entry inhibitor, is characterized in this study as a potent inhibitor of the Dengue virus (DENV). CRDS was identified by in silico blind docking studies to exhibit binding potential to the envelope (E) protein of the DENV. CRDS was shown to inhibit the DENV replication very efficiently in different cells in vitro. Minimal effective concentration of CRDS was as low as 0.1 µg/mL in LLC-MK2 cells, and toxicity was observed only at concentrations over 10 mg/mL. CRDS can also inhibit DENV-1, 3, and 4 efficiently. CRDS did not inhibit the replication of DENV subgenomic replicon. Time of addition experiments demonstrated that the compound not only inhibited viral infection at the host cell binding step, but also at an early post-attachment step of entry (membrane fusion). The direct binding of CRDS to DENV was suggested by an evident reduction in the viral titers after interaction of the virus with CRDS following an ultrafiltration device separation, as well as after virus adsorption to an alkyl CRDS-coated membrane filter. The electron microscopic features also showed that CRDS interacted directly with the viral envelope, and caused changes to the viral surface. CRDS also potently inhibited DENV infection in DC-SIGN expressing cells as well as the antibody-dependent enhancement of DENV-2 infection. Based on these data, a probable binding model of CRDS to DENV E protein was constructed by a flexible receptor and ligand docking study. The binding site of CRDS was predicted to be at the interface between domains II and III of E protein dimer, which is unique to this compound, and is apparently different from the β-OG binding site. Since CRDS has already been tested in humans without serious side effects, its clinical application can be considered.
  • Takeshi Chujo, Takayuki Ohira, Yuriko Sakaguchi, Naoki Goshima, Nobuo Nomura, Asuteka Nagao, Tsutomu Suzuki
    NUCLEIC ACIDS RESEARCH, 40(16) 8033-8047, Sep, 2012  Peer-reviewed
    In human mitochondria, 10 mRNAs species are generated from a long polycistronic precursor that is transcribed from the heavy chain of mitochondrial DNA, in theory yielding equal copy numbers of mRNA molecules. However, the steady-state levels of these mRNAs differ substantially. Through absolute quantification of mRNAs in HeLa cells, we show that the copy numbers of all mitochondrial mRNA species range from 6000 to 51 000 molecules per cell, indicating that mitochondria actively regulate mRNA metabolism. In addition, the copy numbers of mitochondrial mRNAs correlated with their cellular half-life. Previously, mRNAs with longer half-lives were shown to be stabilized by the LRPPRC/SLIRP complex, which we find that cotranscriptionally binds to coding sequences of mRNAs. We observed that the LRPPRC/SLIRP complex suppressed 3' exonucleolytic mRNA degradation mediated by PNPase and SUV3. Moreover, LRPPRC promoted the polyadenylation of mRNAs mediated by mitochondrial poly(A) polymerase (MTPAP) in vitro. These findings provide a framework for understanding the molecular mechanism of mRNA metabolism in human mitochondria.
  • Yukio Maruyama, Yoshifumi Kawamura, Tetsuo Nishikawa, Takao Isogai, Nobuo Nomura, Naoki Goshima
    NUCLEIC ACIDS RESEARCH, 40(D1) D924-D929, Jan, 2012  Peer-reviewed
    The Human Gene and Protein Database (HGPD; http://www.HGPD.jp/) is a unique database that stores information on a set of human Gateway entry clones in addition to protein expression and protein synthesis data. The HGPD was launched in November 2008, and 33 275 human Gateway entry clones have been constructed from the open reading frames (ORFs) of full-length cDNA, thus representing the largest collection in the world. Recently, research objectives have focused on the development of new medicines and the establishment of novel diagnostic methods and medical treatments. And, studies using proteins and protein information, which are closely related to gene function, have been undertaken. For this update, we constructed an additional 9974 human Gateway entry clones, giving a total of 43 249. This set of human Gateway entry clones was named the Human Proteome Expression Resource, known as the 'HuPEX'. In addition, we also classified the clones into 10 groups according to protein function. Moreover, in vivo cellular localization data of proteins for 32 651 human Gateway entry clones were included for retrieval from the HGPD. In 'Information Overview', which presents the search results, the ORF region of each cDNA is now displayed allowing the Gateway entry clones to be searched more easily.
  • Hiroyuki Takeda, Yoshifumi Kawamura, Aya Miura, Masatoshi Mori, Ai Wakamatsu, Jun-ichi Yamamoto, Takao Isogai, Masaki Matsumoto, Keiichi I. Nakayama, Tohru Natsume, Nobuo Nomura, Naoki Goshima
    JOURNAL OF PROTEOME RESEARCH, 9(11) 5982-5993, Nov, 2010  Peer-reviewed
    Sic family kinases (SFKs) are the earliest known family of tyrosine kinases and are widely thought to play essential roles in cellular signal transduction. Although numerous functional analyses have been performed, no study has analyzed the specificity of all SFKs on an equal platform. To gain a better understanding of SFK phosphorylation, we designed a high-throughput in vitro kinase assay on the subproteome scale using surface plasmon resonance. We reacted each of the 11 human SFKs with 519 substrate proteins, and significant phosphorylation was detected in 33.6% (1921) of the total 5709 kinase substrate combinations. A large number of novel phosphorylations were included among them. Many substrates were shown to be phosphorylated by multiple SFKs, which might reflect functional complementarity of SFKs. Clustering analysis of phosphorylation results grouped substrates into 10 categories, while the similarity of SFK catalytic specificity exhibited no significant correlation with that of amino acid sequences. In silico predictions of SRC-specific phosphorylation sites were not consistent with experimental results, implying some unknown SRC recognition modes. In an attempt to find biologically meaningful novel substrates, phosphorylation data were integrated with annotation data. The extensive in vitro data obtained in this study would provide valuable clues for further understanding SFK-mediated signal transduction.
  • Yukio Maruyama, Yoshifumi Kawamura, Takao Isogai, Nobuo Nomura, Naoki Goshima
    Nature proceedings, 10, Oct, 2010  

Misc.

 7

Books and Other Publications

 13

Presentations

 40

Professional Memberships

 2

教育内容・方法の工夫

 1
  • Subject
    重要事項を記載したプリントの配布、重要事項に関する質疑応答等による理解力の向上
    Date(From)
    2013/04/01
    Date(To)
    2013/04/01
    Summary
    (1) 授業の主な内容をプリントにして配布する。(2) 予習が効率的に行えるように配布プリントの原稿を授業の原則1週間前(遅くとも3日前)に学内のネット上にアップしている。(3)また、補足的な内容の資料については、印刷物は配布しないが、これらの原稿も授業の原則1週間前(遅くとも3日前)に学内のネット上にアップしている。


    配布プリントのアップを授業に先だって行うことにより、学生の予習を可能とした。また、欠席した生徒もプリントを入手可能となる。授業の音声をアップすることにより、復習の助けとするととも

資格・免許

 1
  • Subject
    第1種放射線取扱い主任者