CVClient

野村 信夫

ノムラ ノブオ  (Nomura Nobuo)

基本情報

所属
武蔵野大学 人間科学部 人間科学科 教授
学位
博士(京都大学大学院理学研究科)

研究者番号
20147862
J-GLOBAL ID
201701013411747110
researchmap会員ID
B000269551

論文

 143
  • Takeshi Chujo, Takayuki Ohira, Yuriko Sakaguchi, Naoki Goshima, Nobuo Nomura, Asuteka Nagao, Tsutomu Suzuki
    NUCLEIC ACIDS RESEARCH 40(16) 8033-8047 2012年9月  査読有り
    In human mitochondria, 10 mRNAs species are generated from a long polycistronic precursor that is transcribed from the heavy chain of mitochondrial DNA, in theory yielding equal copy numbers of mRNA molecules. However, the steady-state levels of these mRNAs differ substantially. Through absolute quantification of mRNAs in HeLa cells, we show that the copy numbers of all mitochondrial mRNA species range from 6000 to 51 000 molecules per cell, indicating that mitochondria actively regulate mRNA metabolism. In addition, the copy numbers of mitochondrial mRNAs correlated with their cellular half-life. Previously, mRNAs with longer half-lives were shown to be stabilized by the LRPPRC/SLIRP complex, which we find that cotranscriptionally binds to coding sequences of mRNAs. We observed that the LRPPRC/SLIRP complex suppressed 3' exonucleolytic mRNA degradation mediated by PNPase and SUV3. Moreover, LRPPRC promoted the polyadenylation of mRNAs mediated by mitochondrial poly(A) polymerase (MTPAP) in vitro. These findings provide a framework for understanding the molecular mechanism of mRNA metabolism in human mitochondria.
  • 核酸研究 40(D1) D924-D929 2012年1月  査読有り
    Koji Ichiyama, Sindhoora Bhargavi Gopala Reddy, Li Feng Zhang, Wei Xin Chin
  • Hiroyuki Takeda, Yoshifumi Kawamura, Aya Miura, Masatoshi Mori, Ai Wakamatsu, Jun-ichi Yamamoto, Takao Isogai, Masaki Matsumoto, Keiichi I. Nakayama, Tohru Natsume, Nobuo Nomura, Naoki Goshima
    JOURNAL OF PROTEOME RESEARCH 9(11) 5982-5993 2010年11月  査読有り
    Sic family kinases (SFKs) are the earliest known family of tyrosine kinases and are widely thought to play essential roles in cellular signal transduction. Although numerous functional analyses have been performed, no study has analyzed the specificity of all SFKs on an equal platform. To gain a better understanding of SFK phosphorylation, we designed a high-throughput in vitro kinase assay on the subproteome scale using surface plasmon resonance. We reacted each of the 11 human SFKs with 519 substrate proteins, and significant phosphorylation was detected in 33.6% (1921) of the total 5709 kinase substrate combinations. A large number of novel phosphorylations were included among them. Many substrates were shown to be phosphorylated by multiple SFKs, which might reflect functional complementarity of SFKs. Clustering analysis of phosphorylation results grouped substrates into 10 categories, while the similarity of SFK catalytic specificity exhibited no significant correlation with that of amino acid sequences. In silico predictions of SRC-specific phosphorylation sites were not consistent with experimental results, implying some unknown SRC recognition modes. In an attempt to find biologically meaningful novel substrates, phosphorylation data were integrated with annotation data. The extensive in vitro data obtained in this study would provide valuable clues for further understanding SFK-mediated signal transduction.
  • Yukio Maruyama, Yoshifumi Kawamura, Takao Isogai, Nobuo Nomura, Naoki Goshima
    Nature proceedings 10 2010年10月  

MISC

 7

書籍等出版物

 13

講演・口頭発表等

 38

教育内容・方法の工夫

 1
  • 件名
    重要事項を記載したプリントの配布、重要事項に関する質疑応答等による理解力の向上
    年月日(From)
    2013/04/01
    年月日(To)
    2013/04/01
    概要
    (1) 授業の主な内容をプリントにして配布する。(2) 予習が効率的に行えるように配布プリントの原稿を授業の原則1週間前(遅くとも3日前)に学内のネット上にアップしている。(3)また、補足的な内容の資料については、印刷物は配布しないが、これらの原稿も授業の原則1週間前(遅くとも3日前)に学内のネット上にアップしている。


    配布プリントのアップを授業に先だって行うことにより、学生の予習を可能とした。また、欠席した生徒もプリントを入手可能となる。授業の音声をアップすることにより、復習の助けとするととも

資格・免許

 1
  • 件名
    第1種放射線取扱い主任者